Q9QYX2 (RNAS1_MUSSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease pancreatic
EC=3.1.27.5

Alternative name(s):
RNase 1
RNase A

Gene names

Name:

Rnase1

Organism

Mus saxicola (Brown spiny mouse) (Rock-loving mouse)

Taxonomic identifier

10094 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Pyromys

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Endonuclease that catalyzes the cleavage of RNA on the 3' side of pyrimidine nucleotides. Acts on single stranded and double stranded RNA By similarity.
Catalytic activity	Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in Cp or Up with 2',3'-cyclic phosphate intermediates.
Subunit structure	Monomer. Interacts with and forms tight 1:1 complexes with RNH1. Dimerization of two such complexes may occur. Interaction with RNH1 inhibits this protein By similarity.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the pancreatic ribonuclease family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro pancreatic ribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 149	124	Ribonuclease pancreatic		PRO_0000030931
Regions
	Region	66 – 70	5	Substrate binding By similarity
Sites
	Active site	37	1	Proton acceptor By similarity
	Active site	144	1	Proton donor By similarity
	Binding site	32	1	Substrate By similarity
	Binding site	35	1	Substrate By similarity
	Binding site	91	1	Substrate By similarity
Amino acid modifications
	Glycosylation	87	1	N-linked (GlcNAc...) Potential
	Disulfide bond	51 ↔ 109		By similarity
	Disulfide bond	65 ↔ 120		By similarity
	Disulfide bond	83 ↔ 135		By similarity
	Disulfide bond	90 ↔ 97		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QYX2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 00EF21B302181CEA	FASTA	149	16,642
	10 20 30 40 50 60 MGLEKSLMLF PLFVLLLGWV QPSLGRESSA QKFQRQHMDP AGSSSNSPTY CNQMMKRRDM 70 80 90 100 110 120 TKGSCKPVNT FVHEPLADVQ AICSQENVTC KNGKSNCYKS SSALHITDCH LKGNSKYPNC 130 140 DYKTSQYQKQ IIVACEGNPY VPVHFDATV

References

[1]	"The phylogenetic position of 'Acomyinae' (Rodentia, Mammalia) as sister group of a Murinae + Gerbillinae clade: evidence from the nuclear ribonuclease gene." Dubois J.-Y.F., Catzeflis F.M., Beintema J.J. Mol. Phylogenet. Evol. 13:181-192(1999) [PubMed: 10508551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ238700 Genomic DNA. Translation: CAB60003.1.
3D structure databases
ProteinModelPortal	Q9QYX2.
SMR	Q9QYX2. Positions 27-149.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG008396.
Family and domain databases
InterPro	IPR001427. RNaseA. IPR023411. RNaseA_AS. IPR023412. RNaseA_domain. [Graphical view]
Gene3D	G3DSA:3.10.130.10. RNaseA. 1 hit.
PANTHER	PTHR11437. RNaseA. 1 hit.
Pfam	PF00074. RnaseA. 1 hit. [Graphical view]
PRINTS	PR00794. RIBONUCLEASE.
ProDom	PD000535. RNaseA. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00092. RNAse_Pc. 1 hit. [Graphical view]
SUPFAM	SSF54076. RNaseA. 1 hit.
PROSITE	PS00127. RNASE_PANCREATIC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNAS1_MUSSA

Accession

Primary (citable) accession number: Q9QYX2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 2000
Last modified:	May 31, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program