Q9QYW3 (PHOCN_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
MOB-like protein phocein

Alternative name(s):
Class II mMOB1
Mob1 homolog 3
Short name=Mob3
Mps one binder kinase activator-like 3
Phocein
Preimplantation protein 3

Gene names

Name:	Mob4
Synonyms:	Mob3, Mobkl1, Phocn, Prei3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in membrane trafficking, specifically in membrane budding reactions. Ref.3
Subunit structure	Binds STRN, STRN3 and STRN4. Part of a ternary complex containing MOB4/PHOCN, STRN and/or STRN3 and PPA2. Interacts with DNM1 and EPS15. Interacts with nucleoside diphosphate kinase. Interacts with CTTNBP2. Interacts with CTTNBP2NL By similarity. Ref.1 Ref.3 Ref.4
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein. Golgi apparatus › Golgi stack membrane; Peripheral membrane protein. Note: Detected in cell bodies and dendrites of neurons, but not in axons. Ref.1 Ref.3
Tissue specificity	Highly expressed in adrenal gland, spinal cord, brain and cerebellum. Detected at lower levels in heart and skeletal muscle, and at very low levels in spleen, liver and intestine. Ref.1
Post-translational modification	Phosphorylated on serine residues By similarity.
Sequence similarities	Belongs to the MOB1/phocein family.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm Golgi apparatus Membrane
Ligand	Metal-binding Zinc
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	membrane budding Traceable author statement Ref.3. Source: UniProtKB
Cellular_component	Golgi apparatus Inferred from direct assay Ref.3. Source: UniProtKB Golgi cisterna membrane Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from direct assay Ref.1. Source: RGD
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	MOB-like protein phocein		PRO_0000193578
Sites
	Metal binding	92	1	Zinc By similarity
	Metal binding	97	1	Zinc By similarity
	Metal binding	169	1	Zinc By similarity
	Metal binding	174	1	Zinc By similarity
Amino acid modifications
	Modified residue	140	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QYW3 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: D0EF1219711458BA	FASTA	225	26,032
	10 20 30 40 50 60 MVMAEGTAVL RRNRPGTKAQ DFYNWPDESF DEMDSTLAVQ QYIQQNIRAD CSNIDKILEP 70 80 90 100 110 120 PEGQDEGVWK YEHLRQFCLE LNGLAVKLQS ECHPDTCTQM TATEQWIFLC AAHKTPKECP 130 140 150 160 170 180 AIDYTRHTLD GAACLLNSNK YFPSRVSIKE SSVAKLGSVC RRIYRIFSHA YFHHRQIFDE 190 200 210 220 YENETFLCHR FTKFVMKYNL MSKDNLIVPI LEEEVQNSVS GESEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of phocein, a protein found from the Golgi complex to dendritic spines." Baillat G., Moqrich A., Castets F., Baude A., Bailly Y., Benmerah A., Monneron A. Mol. Biol. Cell 12:663-673(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH STRN; STRN3 AND STRN4, TISSUE SPECIFICITY, SUBCELLULAR LOCATION. Strain: Wistar. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
[3]	"Interactions of phocein with nucleoside-diphosphate kinase, Eps15, and Dynamin I." Baillat G., Gaillard S., Castets F., Monneron A. J. Biol. Chem. 277:18961-18966(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH DNM1 AND EPS15, SUBUNIT, SUBCELLULAR LOCATION.
[4]	"CTTNBP2, but not CTTNBP2NL, regulates dendritic spinogenesis and synaptic distribution of the striatin-PP2A complex." Chen Y.K., Chen C.Y., Hu H.T., Hsueh Y.P. Mol. Biol. Cell 23:4383-4392(2012) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH CTTNBP2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ132008 mRNA. Translation: CAB57295.1. BC085708 mRNA. Translation: AAH85708.1.
IPI	IPI00214490.
RefSeq	NP_598212.1. NM_133528.1.
UniGene	Rn.12262.
3D structure databases
ProteinModelPortal	Q9QYW3.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-233176.
STRING	10116.ENSRNOP00000020536.
PTM databases
PhosphoSite	Q9QYW3.
Proteomic databases
PaxDb	Q9QYW3.
PRIDE	Q9QYW3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000020536; ENSRNOP00000020536; ENSRNOG00000014980.
GeneID	171050.
KEGG	rno:171050.
UCSC	RGD:620183. rat.
Organism-specific databases
CTD	25843.
RGD	620183. Mob4.
Phylogenomic databases
eggNOG	NOG297181.
GeneTree	ENSGT00390000012143.
HOGENOM	HOG000253925.
HOVERGEN	HBG052490.
InParanoid	Q9QYW3.
OMA	CRWPDEP.
OrthoDB	EOG4Q2DGB.
Gene expression databases
Genevestigator	Q9QYW3.
GermOnline	ENSRNOG00000014980. Rattus norvegicus.
Family and domain databases
Gene3D	1.20.140.30. 1 hit.
InterPro	IPR005301. Mob1_phocein. [Graphical view]
PANTHER	PTHR22599. PTHR22599. 1 hit.
Pfam	PF03637. Mob1_phocein. 1 hit. [Graphical view]
SUPFAM	SSF101152. Mob1_phocein. 1 hit.
ProtoNet	Search...
Other
NextBio	621589.

Entry information

Entry name

PHOCN_RAT

Accession

Primary (citable) accession number: Q9QYW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program