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Q9QYS2 (GRM3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Metabotropic glutamate receptor 3

Short name=mGluR3
Gene names
Name:Grm3
Synonyms:Gprc1c, Mglur3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length879 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

Subunit structure

Interacts with GRASP By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processadenylate cyclase-inhibiting G-protein coupled glutamate receptor signaling pathway

Inferred from direct assay PubMed 7965099. Source: GOC

regulation of sensory perception of pain

Non-traceable author statement PubMed 11099825. Source: UniProtKB

regulation of synaptic transmission, glutamatergic

Inferred from Biological aspect of Ancestor. Source: RefGenome

sensory perception of pain

Traceable author statement PubMed 11099825. Source: UniProtKB

synaptic transmission, glutamatergic

Traceable author statement PubMed 11591452. Source: UniProtKB

   Cellular_componentastrocyte projection

Inferred from electronic annotation. Source: Ensembl

axon

Inferred from direct assay PubMed 11591452. Source: UniProtKB

dendritic spine

Inferred from direct assay PubMed 11591452. Source: UniProtKB

integral component of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

neuron projection

Inferred from direct assay PubMed 12074840. Source: MGI

plasma membrane

Inferred from direct assay PubMed 11591452. Source: UniProtKB

postsynaptic density

Inferred from direct assay PubMed 15579147. Source: MGI

postsynaptic membrane

Inferred from direct assay PubMed 11591452. Source: UniProtKB

presynaptic active zone

Non-traceable author statement PubMed 11591452. Source: UniProtKB

presynaptic membrane

Inferred from direct assay PubMed 11591452. Source: UniProtKB

   Molecular_functioncalcium channel regulator activity

Inferred from direct assay PubMed 7965099. Source: MGI

group II metabotropic glutamate receptor activity

Inferred from direct assay PubMed 7965099. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 879857Metabotropic glutamate receptor 3
PRO_0000012928

Regions

Topological domain23 – 576554Extracellular Potential
Transmembrane577 – 59923Helical; Name=1; Potential
Topological domain600 – 61314Cytoplasmic Potential
Transmembrane614 – 63421Helical; Name=2; Potential
Topological domain635 – 64511Extracellular Potential
Transmembrane646 – 66419Helical; Name=3; Potential
Topological domain665 – 68824Cytoplasmic Potential
Transmembrane689 – 70921Helical; Name=4; Potential
Topological domain710 – 73425Extracellular Potential
Transmembrane735 – 75622Helical; Name=5; Potential
Topological domain757 – 76913Cytoplasmic Potential
Transmembrane770 – 79223Helical; Name=6; Potential
Topological domain793 – 80210Extracellular Potential
Transmembrane803 – 82826Helical; Name=7; Potential
Topological domain829 – 87951Cytoplasmic Potential
Region172 – 1743Glutamate binding By similarity

Sites

Binding site1511Glutamate By similarity
Binding site2221Glutamate By similarity
Binding site3011Glutamate By similarity
Binding site3891Glutamate By similarity

Amino acid modifications

Glycosylation2091N-linked (GlcNAc...) Potential
Glycosylation2921N-linked (GlcNAc...) Potential
Glycosylation4141N-linked (GlcNAc...) Potential
Glycosylation4391N-linked (GlcNAc...) Potential
Disulfide bond57 ↔ 99 By similarity
Disulfide bond240 ↔ 527 By similarity
Disulfide bond361 ↔ 373 By similarity
Disulfide bond412 ↔ 419 By similarity
Disulfide bond509 ↔ 528 By similarity
Disulfide bond513 ↔ 531 By similarity
Disulfide bond534 ↔ 546 By similarity
Disulfide bond549 ↔ 562 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9QYS2 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: F3A8B26CE96679EF

FASTA87999,114
        10         20         30         40         50         60 
MKMLTRLQVL MLALFSKGFL VSLGDHNFMR REIKIEGDLV LGGLFPINEK GTGTEECGRI 

        70         80         90        100        110        120 
NEDRGIQRLE AMLFAIDEIN KDNYLLPGVK LGVHILDTCS RDTYALEQSL EFVRASLTKV 

       130        140        150        160        170        180 
DEAEYMCPDG SYAIQENIPL LIAGVIGGSY SSVSIQVANL LRLFQIPQIS YASTSAKLSD 

       190        200        210        220        230        240 
KSRYDYFART VPPDFYQAKA MAEILRYFNW TYVSTVASEG DYGETGIEAF EQEARLRNIC 

       250        260        270        280        290        300 
IATAEKVGRS NIRKSYDSVI RELLQKPNAR VVVLFMRSDD SRELIAAASR VNASFTWVAS 

       310        320        330        340        350        360 
DGWGAQESIV KGSEHVAYGA ITLELASHPV RQFDRYFQSL NPYNNHRNPW FRDFWEQKFQ 

       370        380        390        400        410        420 
CSLQNKRNHR QICDKHLAID SSNYEQESKI MFVVNAVYAM AHALHKMQRT LCPNTTKLCD 

       430        440        450        460        470        480 
AMKILDGKKL YKDYLLKINF TAPFNPNKGA DSIVKFDTYG DGMGRYNVFN FQHIGGKYSY 

       490        500        510        520        530        540 
LKVGHWAETL YLDVDSIHWS RNSVPTSQCS DPCAPNEMKN MQPGDVCCWI CIPCEPYEYL 

       550        560        570        580        590        600 
VDEFTCMDCG PGQWPTADLS GCYNLPEDYI RWEDAWAIGP VTIACLGFMC TCIVITVFIK 

       610        620        630        640        650        660 
HNNTPLVKAS GRELCYILLF GVSLSYCMTF FFIAKPSPVI CALRRLGLGT SFAICYSALL 

       670        680        690        700        710        720 
TKTNCIARIF DGVKNGAQRP KFISPSSQVF ICLGLILVQI VMVSVWLILE TPGTRRYTLP 

       730        740        750        760        770        780 
EKRETVILKC NVKDSSMLIS LTYDVVLVIL CTVYAFKTRK CPENFNEAKF IGFTMYTTCI 

       790        800        810        820        830        840 
IWLAFLPIFY VTSSDYRVQT TTMCISVSLS GFVVLGCLFA PKVHIVLFQP QKNVVTHRLH 

       850        860        870 
LNRFSVSGTA TTYSQSSAST YVPTVCNGRE VLDSTTSSL 

« Hide

References

« Hide 'large scale' references
[1]"Structural organization of the mouse metabotropic glutamate receptor subtype 3 gene and its regulation by growth factors in cultured cortical astrocytes."
Minoshima T., Nakanishi S.
J. Biochem. 126:889-896(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF170701 expand/collapse EMBL AC list , AF170697, AF170698, AF170699, AF170700 Genomic DNA. Translation: AAF06741.1.
BC111891 mRNA. Translation: AAI11892.1.
BC113170 mRNA. Translation: AAI13171.1.
CCDSCCDS19090.1.
PIRJC7160.
RefSeqNP_862898.1. NM_181850.2.
UniGeneMm.318966.

3D structure databases

ProteinModelPortalQ9QYS2.
SMRQ9QYS2. Positions 28-834.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9QYS2. 3 interactions.
MINTMINT-4101874.

Chemistry

BindingDBQ9QYS2.
GuidetoPHARMACOLOGY291.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9QYS2.

Proteomic databases

PaxDbQ9QYS2.
PRIDEQ9QYS2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000004076; ENSMUSP00000004076; ENSMUSG00000003974.
GeneID108069.
KEGGmmu:108069.
UCSCuc008wll.1. mouse.

Organism-specific databases

CTD2913.
MGIMGI:1351340. Grm3.

Phylogenomic databases

eggNOGNOG295200.
GeneTreeENSGT00750000117490.
HOGENOMHOG000218635.
HOVERGENHBG107965.
InParanoidQ14DJ9.
KOK04605.
OMACTCIVVT.
OrthoDBEOG7Z0JXG.
PhylomeDBQ9QYS2.
TreeFamTF313240.

Gene expression databases

ArrayExpressQ9QYS2.
BgeeQ9QYS2.
CleanExMM_GRM3.
GenevestigatorQ9QYS2.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001234. GPCR_3_mtglu_rcpt_3.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
PR01053. MTABOTROPC3R.
PR00593. MTABOTROPICR.
SUPFAMSSF53822. SSF53822. 1 hit.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio359984.
PROQ9QYS2.
SOURCESearch...

Entry information

Entry nameGRM3_MOUSE
AccessionPrimary (citable) accession number: Q9QYS2
Secondary accession number(s): Q14DJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2003
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries