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Reviewed, UniProtKB/Swiss-Prot Q9QYR7 (ACOT3_MOUSE)

Last modified November 3, 2009. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Acyl-coenzyme A thioesterase 3
      Short name=Acyl-CoA thioesterase 3
    EC=3.1.2.2
Alternative name(s):
    Peroxisomal acyl-coenzyme A thioester hydrolase 2a
    Peroxisomal long-chain acyl-CoA thioesterase 2
    PTE-Ia
Gene names
Name: Acot3
Synonyms: Pte1a, Pte2, Pte2a
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Mainly active on long-chain acyl-CoAs. May have a function in termination of beta-oxydation of fatty acids.

Catalytic activity

Palmitoyl-CoA + H2O = CoA + palmitate.

Subcellular location

Peroxisome.

Tissue specificity

Widely expressed. Highly expressed in the kidney, expressed at low level in the liver. Isoform 2 is expressed in the kidney, but not in the liver. Isoform 1 is expressed in more liver-specific. Ref.1

Induction

In the liver and kidney, by peroxisome proliferator (such as Clofibrate) treatment, via the peroxisome proliferator-activated receptors (PPARs) or fasting for 24 hours.

Sequence similarities

Belongs to the C/M/P thioester hydrolase family.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9QYR7-1)

Also known as: PTE-Ia 5':1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9QYR7-2)

Also known as: PTE-Ia 5':2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-11: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 432432Acyl-coenzyme A thioesterase 3
PRO_0000202148

Regions

Motif430 – 4323Microbody targeting signal Potential

Sites

Active site2431Charge relay system By similarity
Active site3371Charge relay system By similarity
Active site3711Charge relay system By similarity

Natural variations

Alternative sequence1 – 1111Missing in isoform 2.
VSP_010994

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (PTE-Ia 5':1) [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: BF5888C59C015359

FASTA43247,490
        10         20         30         40         50         60 
MHAFTTQNPN RMAPTVILEP AGGCLCDQPV HIAVRGLAPE QPVTLRSVLR DEKGALFRAH 

        70         80         90        100        110        120 
ARYRADSHGE LDLARTPALG GSFSGLEPMG LLWAMEPDRP FWRLIKRDVQ TPFVVELEVL 

       130        140        150        160        170        180 
DGHEPDGGQR LARAVHERHF MAPGVRRVPV REGRVRATLF LPPGTGPFPG IIDLFGIGSG 

       190        200        210        220        230        240 
LLEYRASLLA GKGFAVMALA YNNYEDLPKD MDIIHLEYFE EAVTYLLSHP QVTGSGVGVL 

       250        260        270        280        290        300 
GISKGGELGF AMASFLKNIT AAVIINGSIS NIGGNLQYKD ETVPSVGINT KRVKRTKDGL 

       310        320        330        340        350        360 
KDIVDLLNNP LEGPDQKSLI PVERSDTAFL FLVGQDDHNW KSEFYAREAS KRLQAHGKEK 

       370        380        390        400        410        420 
PQIICYPETG HHIEPPYFPL CKASLNSLVG GPVIWGGEPR AHAMAQVDAW QQLQTFFHNH 

       430 
LDGKKKTIPA KL

« Hide

Isoform 2 (PTE-Ia 5':2).

Checksum: 4146258877CBDB88
Show »

FASTA42146,191

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References

[1]"Peroxisome proliferator-induced long chain acyl-CoA thioesterases comprise a highly conserved novel multi-gene family involved in lipid metabolism."
Hunt M.C., Nousiainen S.E.B., Huttunen M.K., Orii K.E., Svensson L.T., Alexson S.E.H.
J. Biol. Chem. 274:34317-34326(1999) [PubMed: 10567408] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: C57BL/6.
[2]"Molecular cloning and characterization of two mouse peroxisome proliferator-activated receptor alpha (PPARalpha)-regulated peroxisomal acyl-CoA thioesterases."
Westin M.A.K., Alexson S.E.H., Hunt M.C.
J. Biol. Chem. 279:21841-21848(2004) [PubMed: 15007068] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: 129/Sv.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF180804, AF180802, AF180803 Genomic DNA. Translation: AAF13873.1.
AY563098 mRNA. Translation: AAS75456.1.
IPIIPI00136676.
IPI00454167.
RefSeqNP_599007.1.
UniGeneMm.202331

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGQ9QYR7.

PTM databases

PhosphoSiteQ9QYR7.

Proteomic databases

PRIDEQ9QYR7.

Genome annotation databases

EnsemblENSMUST00000021653; ENSMUSP00000021653; ENSMUSG00000021228; Mus musculus. [Genome view]
GeneID171281.
KEGGmmu:171281.
UCSCuc007oef.1. mouse.
uc007oeg.1. mouse.

Organism-specific databases

CTD171281.
MGIMGI:2159619. Acot3.

Phylogenomic databases

HOGENOMQ9QYR7.
HOVERGENQ9QYR7.
OMALEPAGGC.

Enzyme and pathway databases

BRENDA3.1.2.2. 244.

Gene expression databases

ArrayExpressQ9QYR7.
BgeeQ9QYR7.
CleanExMM_ACOT3.
GenevestigatorQ9QYR7.
GermOnlineENSMUSG00000021228. Mus musculus.

Family and domain databases

InterProIPR016662. Acyl-CoA_thioEstase_long-chain.
IPR014940. BAAT_C.
IPR006862. Thio_Ohase/aa_AcTrfase.
[Graphical view]
PfamPF08840. BAAT_C. 1 hit.
PF04775. Bile_Hydr_Trans. 1 hit.
[Graphical view]
PIRSFPIRSF016521. Acyl-CoA_hydro. 1 hit.
ProtoNetSearch...

Other Resources

NextBio370947.
SOURCESearch...

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Entry information

Entry nameACOT3_MOUSE
AccessionPrimary (citable) accession number: Q9QYR7
Secondary accession number(s): Q6Q2Z7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: May 1, 2000
Last modified: November 3, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents