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Protein

5-azacytidine-induced protein 2

Gene

Azi2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.1 Publication

GO - Biological processi

  • cytokine production Source: MGI
  • dendritic cell differentiation Source: MGI
  • dendritic cell proliferation Source: MGI
  • I-kappaB kinase/NF-kappaB signaling Source: MGI
  • interferon-alpha production Source: MGI
  • interferon-gamma production Source: MGI
  • interleukin-6 production Source: MGI
  • mitotic cell cycle Source: MGI
  • T cell activation Source: MGI
  • tumor necrosis factor production Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
5-azacytidine-induced protein 2
Alternative name(s):
NF-kappa-B-activating kinase-associated protein 1
Short name:
Nak-associated protein 1
Gene namesi
Name:Azi2
Synonyms:Az2, Nap1, Tbkp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1351332. Azi2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi236 – 2361Y → A: Abolishes interaction with TBK1 but not with IKBKE. 1 Publication
Mutagenesisi237 – 2371W → A: No effect on interaction with TBK1 and IKBKE. 1 Publication
Mutagenesisi238 – 2381E → A: No effect on interaction with TBK1 and IKBKE. 1 Publication
Mutagenesisi253 – 2531Q → A: Abolishes interaction with TBK1 and IKBKE. 1 Publication
Mutagenesisi257 – 2571L → A: Abolishes interaction with TBK1 and IKBKE. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4054055-azacytidine-induced protein 2PRO_0000280604Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei331 – 3311PhosphoserineBy similarity
Modified residuei366 – 3661PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9QYP6.
MaxQBiQ9QYP6.
PaxDbiQ9QYP6.
PeptideAtlasiQ9QYP6.
PRIDEiQ9QYP6.

PTM databases

iPTMnetiQ9QYP6.
PhosphoSiteiQ9QYP6.

Expressioni

Tissue specificityi

Testis, ovary, heart, lung, kidney and brain. Expressed mainly in the spermatocytes or spermatids in the testis.1 Publication

Inductioni

By 5-azacytidine.1 Publication

Gene expression databases

BgeeiQ9QYP6.
CleanExiMM_AZI2.
ExpressionAtlasiQ9QYP6. baseline and differential.
GenevisibleiQ9QYP6. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with IKBKE, TBK1 and TICAM1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
APBA3O960182EBI-6115874,EBI-6115839From a different organism.
MIB1Q86YT62EBI-6115874,EBI-2129148From a different organism.
MTPAPQ9NVV42EBI-6115874,EBI-2556166From a different organism.
N4BP1O751132EBI-6115874,EBI-5278391From a different organism.
RNF31Q96EP02EBI-6115874,EBI-948111From a different organism.
TRAF3Q131142EBI-6115874,EBI-357631From a different organism.
TXLNAP402222EBI-6115874,EBI-359793From a different organism.

Protein-protein interaction databases

BioGridi205136. 1 interaction.
IntActiQ9QYP6. 11 interactions.
STRINGi10090.ENSMUSP00000044350.

Structurei

3D structure databases

ProteinModelPortaliQ9QYP6.
SMRiQ9QYP6. Positions 45-71.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 198198HomodimerizationAdd
BLAST
Regioni229 – 27042Interaction with TBK1 and IKBKEAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili40 – 197158Sequence analysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IG4Z. Eukaryota.
ENOG4111IT0. LUCA.
GeneTreeiENSGT00390000001279.
HOGENOMiHOG000013059.
HOVERGENiHBG067551.
InParanoidiQ9QYP6.
KOiK12651.
OMAiTATYKRH.
OrthoDBiEOG75XGKS.
PhylomeDBiQ9QYP6.
TreeFamiTF331289.

Family and domain databases

InterProiIPR024581. TBD.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QYP6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDTLVEDDIC ILNHEKAHRR EAVTPLSAYP GDESVASHFA LVTAYEDIKK
60 70 80 90 100
RLKDSEKENS FLKKRIRALE ERLVGARADE ETSSVGREQV NKAYHAYREV
110 120 130 140 150
CIDRDNLKNQ LEKINKDNSE SLKMLNEQLQ SKEVELLQLR TEVETQQVMR
160 170 180 190 200
NLNPPSSSWE VEKLSCDLKI HGLEQELGLL RKECSDLRTE LQKARQTGPP
210 220 230 240 250
QEDILQGRDV IRPSLSREEH VPHQGLHHSD NMQHAYWELK REMSNLHLVT
260 270 280 290 300
QVQAELLRKL KTSAAVKKAC TPVGCVEDLG RDSTKLHLTN FTATYKRHPS
310 320 330 340 350
LSPNGKAPCY APSSPLPGDR KVFSDKAVLQ SWTDNERLVP NDGADFPEHS
360 370 380 390 400
SYGRNSLEDN SWVFPSPPKS SETAFGENKS KILPLSNLPP LHYLDQQNQN

CLYKS
Length:405
Mass (Da):46,091
Last modified:May 1, 2000 - v1
Checksum:i5EBD91369CBCB89F
GO
Isoform 2 (identifier: Q9QYP6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     269-283: ACTPVGCVEDLGRDS → GKSLLAANADSSQTA
     284-405: Missing.

Show »
Length:283
Mass (Da):32,389
Checksum:iB51C5A60133C9236
GO
Isoform 3 (identifier: Q9QYP6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     115-405: Missing.

Note: May be produced at very low levels due to a premature stop codon in the mRNA, leading to nonsense-mediated mRNA decay.
Show »
Length:114
Mass (Da):13,130
Checksum:iB80F9AF6016B8487
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei115 – 405291Missing in isoform 3. 1 PublicationVSP_023822Add
BLAST
Alternative sequencei269 – 28315ACTPV…LGRDS → GKSLLAANADSSQTA in isoform 2. 1 PublicationVSP_023823Add
BLAST
Alternative sequencei284 – 405122Missing in isoform 2. 1 PublicationVSP_023824Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007141 mRNA. Translation: BAA88213.1.
AK004992 mRNA. Translation: BAB23727.1.
AK015942 mRNA. Translation: BAB30044.1.
AK030076 mRNA. Translation: BAC26770.1.
AK036102 mRNA. Translation: BAE43294.1.
AK153477 mRNA. Translation: BAE32027.1.
AK165549 mRNA. Translation: BAE38249.1.
AK167783 mRNA. Translation: BAE39815.1.
BC006851 mRNA. Translation: AAH06851.1.
CCDSiCCDS40799.1. [Q9QYP6-1]
CCDS52959.1. [Q9QYP6-2]
RefSeqiNP_001041611.1. NM_001048146.2. [Q9QYP6-2]
NP_001273436.1. NM_001286507.1.
NP_001273437.1. NM_001286508.1. [Q9QYP6-1]
NP_038755.1. NM_013727.4. [Q9QYP6-1]
UniGeneiMm.92705.

Genome annotation databases

EnsembliENSMUST00000044454; ENSMUSP00000044350; ENSMUSG00000039285. [Q9QYP6-1]
ENSMUST00000133580; ENSMUSP00000118765; ENSMUSG00000039285. [Q9QYP6-1]
ENSMUST00000134433; ENSMUSP00000114980; ENSMUSG00000039285. [Q9QYP6-2]
ENSMUST00000154583; ENSMUSP00000122063; ENSMUSG00000039285. [Q9QYP6-3]
GeneIDi27215.
KEGGimmu:27215.
UCSCiuc009rzi.2. mouse. [Q9QYP6-2]
uc009rzj.2. mouse. [Q9QYP6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB007141 mRNA. Translation: BAA88213.1.
AK004992 mRNA. Translation: BAB23727.1.
AK015942 mRNA. Translation: BAB30044.1.
AK030076 mRNA. Translation: BAC26770.1.
AK036102 mRNA. Translation: BAE43294.1.
AK153477 mRNA. Translation: BAE32027.1.
AK165549 mRNA. Translation: BAE38249.1.
AK167783 mRNA. Translation: BAE39815.1.
BC006851 mRNA. Translation: AAH06851.1.
CCDSiCCDS40799.1. [Q9QYP6-1]
CCDS52959.1. [Q9QYP6-2]
RefSeqiNP_001041611.1. NM_001048146.2. [Q9QYP6-2]
NP_001273436.1. NM_001286507.1.
NP_001273437.1. NM_001286508.1. [Q9QYP6-1]
NP_038755.1. NM_013727.4. [Q9QYP6-1]
UniGeneiMm.92705.

3D structure databases

ProteinModelPortaliQ9QYP6.
SMRiQ9QYP6. Positions 45-71.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205136. 1 interaction.
IntActiQ9QYP6. 11 interactions.
STRINGi10090.ENSMUSP00000044350.

PTM databases

iPTMnetiQ9QYP6.
PhosphoSiteiQ9QYP6.

Proteomic databases

EPDiQ9QYP6.
MaxQBiQ9QYP6.
PaxDbiQ9QYP6.
PeptideAtlasiQ9QYP6.
PRIDEiQ9QYP6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000044454; ENSMUSP00000044350; ENSMUSG00000039285. [Q9QYP6-1]
ENSMUST00000133580; ENSMUSP00000118765; ENSMUSG00000039285. [Q9QYP6-1]
ENSMUST00000134433; ENSMUSP00000114980; ENSMUSG00000039285. [Q9QYP6-2]
ENSMUST00000154583; ENSMUSP00000122063; ENSMUSG00000039285. [Q9QYP6-3]
GeneIDi27215.
KEGGimmu:27215.
UCSCiuc009rzi.2. mouse. [Q9QYP6-2]
uc009rzj.2. mouse. [Q9QYP6-1]

Organism-specific databases

CTDi64343.
MGIiMGI:1351332. Azi2.

Phylogenomic databases

eggNOGiENOG410IG4Z. Eukaryota.
ENOG4111IT0. LUCA.
GeneTreeiENSGT00390000001279.
HOGENOMiHOG000013059.
HOVERGENiHBG067551.
InParanoidiQ9QYP6.
KOiK12651.
OMAiTATYKRH.
OrthoDBiEOG75XGKS.
PhylomeDBiQ9QYP6.
TreeFamiTF331289.

Miscellaneous databases

ChiTaRSiAzi2. mouse.
PROiQ9QYP6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QYP6.
CleanExiMM_AZI2.
ExpressionAtlasiQ9QYP6. baseline and differential.
GenevisibleiQ9QYP6. MM.

Family and domain databases

InterProiIPR024581. TBD.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of the novel cDNA of a gene of which expression is induced by a demethylating stimulus."
    Miyagawa J., Muguruma M., Aoto H., Suetake I., Nakamura M., Tajima S.
    Gene 240:289-295(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), INDUCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Testis.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: C57BL/6J and DBA/2.
    Tissue: Bone marrow, Cerebellum, Liver and Testis.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  4. "SINTBAD, a novel component of innate antiviral immunity, shares a TBK1-binding domain with NAP1 and TANK."
    Ryzhakov G., Randow F.
    EMBO J. 26:3180-3190(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH IKBKE AND TBK1, HOMODIMER, MUTAGENESIS OF TYR-236; TRP-237; GLU-238; GLN-253 AND LEU-257.
  5. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 241-258, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.

Entry informationi

Entry nameiAZI2_MOUSE
AccessioniPrimary (citable) accession number: Q9QYP6
Secondary accession number(s): Q3TIN1, Q3V3R2, Q9CUI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.