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Protein

Histamine H3 receptor

Gene

Hrh3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist).

GO - Molecular functioni

  • drug binding Source: RGD
  • G-protein coupled acetylcholine receptor activity Source: GO_Central
  • histamine receptor activity Source: RGD

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
  • brain development Source: RGD
  • cognition Source: RGD
  • drinking behavior Source: RGD
  • eating behavior Source: RGD
  • learning Source: RGD
  • memory Source: RGD
  • negative regulation of adenylate cyclase activity Source: RGD
  • negative regulation of blood pressure Source: RGD
  • negative regulation of gamma-aminobutyric acid secretion Source: RGD
  • negative regulation of glutamate secretion Source: RGD
  • negative regulation of serotonin secretion Source: RGD
  • phospholipase C-activating G-protein coupled acetylcholine receptor signaling pathway Source: GO_Central
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of epithelial cell proliferation Source: RGD
  • regulation of neurotransmitter levels Source: InterPro
  • regulation of norepinephrine secretion Source: RGD
  • response to organic cyclic compound Source: RGD
  • synaptic transmission, cholinergic Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Histamine H3 receptor
Short name:
H3R
Short name:
HH3R
Gene namesi
Name:Hrh3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620630. Hrh3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 39ExtracellularSequence analysisAdd BLAST39
Transmembranei40 – 60Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini61 – 70CytoplasmicSequence analysis10
Transmembranei71 – 91Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini92 – 108ExtracellularSequence analysisAdd BLAST17
Transmembranei109 – 129Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini130 – 156CytoplasmicSequence analysisAdd BLAST27
Transmembranei157 – 177Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini178 – 196ExtracellularSequence analysisAdd BLAST19
Transmembranei197 – 217Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini218 – 359CytoplasmicSequence analysisAdd BLAST142
Transmembranei360 – 380Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini381 – 396ExtracellularSequence analysisAdd BLAST16
Transmembranei397 – 417Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini418 – 445CytoplasmicSequence analysisAdd BLAST28

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • myelin sheath Source: RGD
  • neuron projection Source: RGD
  • plasma membrane Source: RGD
  • synapse Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4124.
GuidetoPHARMACOLOGYi264.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000696921 – 445Histamine H3 receptorAdd BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi11N-linked (GlcNAc...)Sequence analysis1
Modified residuei439PhosphoserineBy similarity1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ9QYN8.

PTM databases

PhosphoSitePlusiQ9QYN8.

Expressioni

Tissue specificityi

Expressed abundantly in brain, most notably throughout the thalamus, the ventromedial hypothalamus and the caudate nucleus. Isoform 1 is largely predominant in all tissues.1 Publication

Gene expression databases

BgeeiENSRNOG00000008080.

Interactioni

Chemistry databases

BindingDBiQ9QYN8.

Structurei

3D structure databases

ProteinModelPortaliQ9QYN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi20 – 23Poly-Ala4

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000048707.
HOVERGENiHBG102132.
InParanoidiQ9QYN8.
KOiK04151.
PhylomeDBiQ9QYN8.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003980. Histamine_H3_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01471. HISTAMINEH3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q9QYN8-1) [UniParc]FASTAAdd to basket
Also known as: H3L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERAPPDGLM NASGTLAGEA AAAGGARGFS AAWTAVLAAL MALLIVATVL
60 70 80 90 100
GNALVMLAFV ADSSLRTQNN FFLLNLAISD FLVGAFCIPL YVPYVLTGRW
110 120 130 140 150
TFGRGLCKLW LVVDYLLCAS SVFNIVLISY DRFLSVTRAV SYRAQQGDTR
160 170 180 190 200
RAVRKMALVW VLAFLLYGPA ILSWEYLSGG SSIPEGHCYA EFFYNWYFLI
210 220 230 240 250
TASTLEFFTP FLSVTFFNLS IYLNIQRRTR LRLDGGREAG PEPPPDAQPS
260 270 280 290 300
PPPAPPSCWG CWPKGHGEAM PLHRYGVGEA GPGVEAGEAA LGGGSGGGAA
310 320 330 340 350
ASPTSSSGSS SRGTERPRSL KRGSKPSASS ASLEKRMKMV SQSITQRFRL
360 370 380 390 400
SRDKKVAKSL AIIVSIFGLC WAPYTLLMII RAACHGRCIP DYWYETSFWL
410 420 430 440
LWANSAVNPV LYPLCHYSFR RAFTKLLCPQ KLKVQPHGSL EQCWK
Length:445
Mass (Da):48,588
Last modified:May 1, 2000 - v1
Checksum:i63DFEFC21758FE5B
GO
Isoform 2 (identifier: Q9QYN8-2) [UniParc]FASTAAdd to basket
Also known as: H3S

The sequence of this isoform differs from the canonical sequence as follows:
     274-305: Missing.

Show »
Length:413
Mass (Da):45,889
Checksum:i71995CFD1CDBA6C0
GO
Isoform 3 (identifier: Q9QYN8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     274-321: Missing.

Show »
Length:397
Mass (Da):44,215
Checksum:iBC3C2F482E55B840
GO
Isoform 4 (identifier: Q9QYN8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     274-321: Missing.
     393-445: WYETSFWLLW...PHGSLEQCWK → CVERLGKLEA...IRIWGWVVMG

Show »
Length:449
Mass (Da):49,849
Checksum:iDB01D1481F93CECE
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001888274 – 321Missing in isoform 3 and isoform 4. 1 PublicationAdd BLAST48
Alternative sequenceiVSP_001887274 – 305Missing in isoform 2. 2 PublicationsAdd BLAST32
Alternative sequenceiVSP_001889393 – 445WYETS…EQCWK → CVERLGKLEASLLLPLWMFS GRWRRRKHVCELDVPWMFNQ ERQNCRGARGWIGRCGLPRP PPSVLQLPAEPRQLLLPAPP PGLGRWPCPACPVCTIRIWG WVVMG in isoform 4. 1 PublicationAdd BLAST53

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237919 mRNA. Translation: AAF82086.1.
AY009370 mRNA. Translation: AAK02069.1.
AB015646 mRNA. Translation: BAA88765.1.
AB015646 mRNA. Translation: BAA88766.1.
AB015646 mRNA. Translation: BAA88767.1.
AB015646 mRNA. Translation: BAA88768.1.
RefSeqiNP_001257493.1. NM_001270564.1. [Q9QYN8-2]
NP_001257494.1. NM_001270565.1. [Q9QYN8-3]
NP_001257497.1. NM_001270568.1. [Q9QYN8-4]
NP_445958.1. NM_053506.2. [Q9QYN8-1]
UniGeneiRn.7617.

Genome annotation databases

GeneIDi85268.
KEGGirno:85268.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237919 mRNA. Translation: AAF82086.1.
AY009370 mRNA. Translation: AAK02069.1.
AB015646 mRNA. Translation: BAA88765.1.
AB015646 mRNA. Translation: BAA88766.1.
AB015646 mRNA. Translation: BAA88767.1.
AB015646 mRNA. Translation: BAA88768.1.
RefSeqiNP_001257493.1. NM_001270564.1. [Q9QYN8-2]
NP_001257494.1. NM_001270565.1. [Q9QYN8-3]
NP_001257497.1. NM_001270568.1. [Q9QYN8-4]
NP_445958.1. NM_053506.2. [Q9QYN8-1]
UniGeneiRn.7617.

3D structure databases

ProteinModelPortaliQ9QYN8.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiQ9QYN8.
ChEMBLiCHEMBL4124.
GuidetoPHARMACOLOGYi264.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSitePlusiQ9QYN8.

Proteomic databases

PRIDEiQ9QYN8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi85268.
KEGGirno:85268.

Organism-specific databases

CTDi11255.
RGDi620630. Hrh3.

Phylogenomic databases

HOGENOMiHOG000048707.
HOVERGENiHBG102132.
InParanoidiQ9QYN8.
KOiK04151.
PhylomeDBiQ9QYN8.

Miscellaneous databases

PROiQ9QYN8.

Gene expression databases

BgeeiENSRNOG00000008080.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003980. Histamine_H3_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01471. HISTAMINEH3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHRH3_RAT
AccessioniPrimary (citable) accession number: Q9QYN8
Secondary accession number(s): Q9QYN6, Q9QYN7, Q9QYN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Proxyfan acts as a potent neutral antagonist while thioperamide, ciproxifan and FUB465 act as potent inverse agonists.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.