Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Histamine H3 receptor

Gene

Hrh3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

The H3 subclass of histamine receptors could mediate the histamine signals in CNS and peripheral nervous system. Signals through the inhibition of adenylate cyclase and displays high constitutive activity (spontaneous activity in the absence of agonist).

GO - Molecular functioni

  • drug binding Source: RGD
  • histamine receptor activity Source: RGD

GO - Biological processi

  • brain development Source: RGD
  • cognition Source: RGD
  • drinking behavior Source: RGD
  • eating behavior Source: RGD
  • learning Source: RGD
  • memory Source: RGD
  • negative regulation of adenylate cyclase activity Source: RGD
  • negative regulation of blood pressure Source: RGD
  • negative regulation of gamma-aminobutyric acid secretion Source: RGD
  • negative regulation of glutamate secretion Source: RGD
  • negative regulation of serotonin secretion Source: RGD
  • positive regulation of cytosolic calcium ion concentration Source: RGD
  • positive regulation of epithelial cell proliferation Source: RGD
  • regulation of neurotransmitter levels Source: InterPro
  • regulation of norepinephrine secretion Source: RGD
  • response to organic cyclic compound Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Histamine H3 receptor
Short name:
H3R
Short name:
HH3R
Gene namesi
Name:Hrh3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620630. Hrh3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3939ExtracellularSequence analysisAdd
BLAST
Transmembranei40 – 6021Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini61 – 7010CytoplasmicSequence analysis
Transmembranei71 – 9121Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini92 – 10817ExtracellularSequence analysisAdd
BLAST
Transmembranei109 – 12921Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini130 – 15627CytoplasmicSequence analysisAdd
BLAST
Transmembranei157 – 17721Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini178 – 19619ExtracellularSequence analysisAdd
BLAST
Transmembranei197 – 21721Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini218 – 359142CytoplasmicSequence analysisAdd
BLAST
Transmembranei360 – 38021Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini381 – 39616ExtracellularSequence analysisAdd
BLAST
Transmembranei397 – 41721Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini418 – 44528CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • myelin sheath Source: RGD
  • neuron projection Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4124.
GuidetoPHARMACOLOGYi264.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 445445Histamine H3 receptorPRO_0000069692Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi11 – 111N-linked (GlcNAc...)Sequence analysis
Modified residuei439 – 4391PhosphoserineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiQ9QYN8.

PTM databases

PhosphoSiteiQ9QYN8.

Expressioni

Tissue specificityi

Expressed abundantly in brain, most notably throughout the thalamus, the ventromedial hypothalamus and the caudate nucleus. Isoform 1 is largely predominant in all tissues.1 Publication

Interactioni

Chemistry

BindingDBiQ9QYN8.

Structurei

3D structure databases

ProteinModelPortaliQ9QYN8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi20 – 234Poly-Ala

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000048707.
HOVERGENiHBG102132.
InParanoidiQ9QYN8.
KOiK04151.
OMAiCWGCWQK.
OrthoDBiEOG73804R.
PhylomeDBiQ9QYN8.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003980. Histamine_H3_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01471. HISTAMINEH3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9QYN8-1) [UniParc]FASTAAdd to basket

Also known as: H3L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MERAPPDGLM NASGTLAGEA AAAGGARGFS AAWTAVLAAL MALLIVATVL
60 70 80 90 100
GNALVMLAFV ADSSLRTQNN FFLLNLAISD FLVGAFCIPL YVPYVLTGRW
110 120 130 140 150
TFGRGLCKLW LVVDYLLCAS SVFNIVLISY DRFLSVTRAV SYRAQQGDTR
160 170 180 190 200
RAVRKMALVW VLAFLLYGPA ILSWEYLSGG SSIPEGHCYA EFFYNWYFLI
210 220 230 240 250
TASTLEFFTP FLSVTFFNLS IYLNIQRRTR LRLDGGREAG PEPPPDAQPS
260 270 280 290 300
PPPAPPSCWG CWPKGHGEAM PLHRYGVGEA GPGVEAGEAA LGGGSGGGAA
310 320 330 340 350
ASPTSSSGSS SRGTERPRSL KRGSKPSASS ASLEKRMKMV SQSITQRFRL
360 370 380 390 400
SRDKKVAKSL AIIVSIFGLC WAPYTLLMII RAACHGRCIP DYWYETSFWL
410 420 430 440
LWANSAVNPV LYPLCHYSFR RAFTKLLCPQ KLKVQPHGSL EQCWK
Length:445
Mass (Da):48,588
Last modified:May 1, 2000 - v1
Checksum:i63DFEFC21758FE5B
GO
Isoform 2 (identifier: Q9QYN8-2) [UniParc]FASTAAdd to basket

Also known as: H3S

The sequence of this isoform differs from the canonical sequence as follows:
     274-305: Missing.

Show »
Length:413
Mass (Da):45,889
Checksum:i71995CFD1CDBA6C0
GO
Isoform 3 (identifier: Q9QYN8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     274-321: Missing.

Show »
Length:397
Mass (Da):44,215
Checksum:iBC3C2F482E55B840
GO
Isoform 4 (identifier: Q9QYN8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     274-321: Missing.
     393-445: WYETSFWLLW...PHGSLEQCWK → CVERLGKLEA...IRIWGWVVMG

Show »
Length:449
Mass (Da):49,849
Checksum:iDB01D1481F93CECE
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei274 – 32148Missing in isoform 3 and isoform 4. 1 PublicationVSP_001888Add
BLAST
Alternative sequencei274 – 30532Missing in isoform 2. 2 PublicationsVSP_001887Add
BLAST
Alternative sequencei393 – 44553WYETS…EQCWK → CVERLGKLEASLLLPLWMFS GRWRRRKHVCELDVPWMFNQ ERQNCRGARGWIGRCGLPRP PPSVLQLPAEPRQLLLPAPP PGLGRWPCPACPVCTIRIWG WVVMG in isoform 4. 1 PublicationVSP_001889Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237919 mRNA. Translation: AAF82086.1.
AY009370 mRNA. Translation: AAK02069.1.
AB015646 mRNA. Translation: BAA88765.1.
AB015646 mRNA. Translation: BAA88766.1.
AB015646 mRNA. Translation: BAA88767.1.
AB015646 mRNA. Translation: BAA88768.1.
RefSeqiNP_001257493.1. NM_001270564.1. [Q9QYN8-2]
NP_001257494.1. NM_001270565.1. [Q9QYN8-3]
NP_001257497.1. NM_001270568.1. [Q9QYN8-4]
NP_445958.1. NM_053506.2. [Q9QYN8-1]
UniGeneiRn.7617.

Genome annotation databases

GeneIDi85268.
KEGGirno:85268.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF237919 mRNA. Translation: AAF82086.1.
AY009370 mRNA. Translation: AAK02069.1.
AB015646 mRNA. Translation: BAA88765.1.
AB015646 mRNA. Translation: BAA88766.1.
AB015646 mRNA. Translation: BAA88767.1.
AB015646 mRNA. Translation: BAA88768.1.
RefSeqiNP_001257493.1. NM_001270564.1. [Q9QYN8-2]
NP_001257494.1. NM_001270565.1. [Q9QYN8-3]
NP_001257497.1. NM_001270568.1. [Q9QYN8-4]
NP_445958.1. NM_053506.2. [Q9QYN8-1]
UniGeneiRn.7617.

3D structure databases

ProteinModelPortaliQ9QYN8.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiQ9QYN8.
ChEMBLiCHEMBL4124.
GuidetoPHARMACOLOGYi264.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9QYN8.

Proteomic databases

PRIDEiQ9QYN8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi85268.
KEGGirno:85268.

Organism-specific databases

CTDi11255.
RGDi620630. Hrh3.

Phylogenomic databases

HOGENOMiHOG000048707.
HOVERGENiHBG102132.
InParanoidiQ9QYN8.
KOiK04151.
OMAiCWGCWQK.
OrthoDBiEOG73804R.
PhylomeDBiQ9QYN8.

Miscellaneous databases

PROiQ9QYN8.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003980. Histamine_H3_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01471. HISTAMINEH3R.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of rat histamine H3 receptor reveals distinct species pharmacological profiles."
    Lovenberg T.W., Pyati J., Chang H., Wilson S.J., Erlander M.G.
    J. Pharmacol. Exp. Ther. 293:771-778(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Hypothalamus.
  2. "High constitutive activity of native H3 receptors regulates histamine neurons in brain."
    Morisset S., Rouleau A., Ligneau X., Gbahou F., Tardivel-Lacombe J., Stark H., Schunack W., Ganellin C.R., Schwartz J.-C., Arrang J.-M.
    Nature 408:860-864(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Corpus striatum.
  3. "Cloning of a novel G protein-coupled receptor."
    Itadani H., Takimura T., Nakamura T., Ohta M.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4).
  4. "Cloning and functional expression of the human histamine H3 receptor."
    Lovenberg T.W., Roland B.L., Wilson S.J., Jiang X., Pyati J., Huvar A., Jackson M.R., Erlander M.G.
    Mol. Pharmacol. 55:1101-1107(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiHRH3_RAT
AccessioniPrimary (citable) accession number: Q9QYN8
Secondary accession number(s): Q9QYN6, Q9QYN7, Q9QYN9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Proxyfan acts as a potent neutral antagonist while thioperamide, ciproxifan and FUB465 act as potent inverse agonists.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.