Q9QYM9 (TEFF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tomoregulin-2
Short name=TR-2

Alternative name(s):
Transmembrane protein with EGF-like and two follistatin-like domains

Gene names

Name:	Tmeff2
ORF Names:	MNCb-1026

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May be a survival factor for hippocampal and mesencephalic neurons. The shedded form may up-regulate cell proliferation By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Tissue specificity	Widely expressed in the brain. In the olfactory bulb expressed in mitral cell, granule, and glomerular layers. In the hippocampus expressed in hippocampal cornu ammonis, pyramidal layer, dentate gyrus, and substantia nigra pars compacta. Ref.1
Developmental stage	First detected at E11, reaches a maximum at E15, and remains constant through E17. Ref.5
Post-translational modification	N-glycosylated. Contains chondroitin sulfate glycosaminoglycans By similarity. A soluble form (TMEFF2-ECD) is produced by proteolytic shedding. This shedding can be induced by phorbol ester or proinflammatory cytokines such as TNFalpha, and is mediated by a metalloproteinase ADAM By similarity.
Sequence similarities	Belongs to the tomoregulin family. Contains 1 EGF-like domain. Contains 2 Kazal-like domains.
Sequence caution	The sequence BAA95049.1 differs from that shown. Reason: Frameshift at position 209.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	EGF-like domain Repeat Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9QYM9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9QYM9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     147-262: VHEGSGETSQ...KLEESAREHH → EKFSKVMLIL...TVLKGTIPVP

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 40	40	By similarity
	Chain	41 – 374	334	Tomoregulin-2		PRO_0000016588
Regions
	Topological domain	41 – 320	280	Extracellular Potential
	Transmembrane	321 – 341	21	Helical; Potential
	Topological domain	342 – 374	33	Cytoplasmic Potential
	Domain	85 – 137	53	Kazal-like 1
	Domain	176 – 229	54	Kazal-like 2
	Domain	261 – 301	41	EGF-like
	Region	303 – 320	18	Required for shedding By similarity
	Compositional bias	231 – 235	5	Poly-Thr
Amino acid modifications
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	230	1	N-linked (GlcNAc...) Potential
	Disulfide bond	265 ↔ 278		By similarity
	Disulfide bond	273 ↔ 289		By similarity
	Disulfide bond	291 ↔ 300		By similarity
Natural variations
	Alternative sequence	147 – 262	116	VHEGS…AREHH → EKFSKVMLILKPCTASSLEE TNSKEIILCNPSNTHLLKKN ENANLTCWSEPFLQAYGLMR TWNSRQREAAKGITETRFLP PPSAYCLGIFIGANVKINSE ALFRQWTVLKGTIPVP in isoform 2.		VSP_014314

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 2C16FEF2C3A69385	FASTA	374	41,415
	10 20 30 40 50 60 MVLWESPRQC SSWTLCEGFC WLLLLPVTLL IIARPVKLAA FPTSLSDCQT PTGWNCSGYD 70 80 90 100 110 120 DRENDLFLCD TNTCKFDGEC LRIGDTVTCV CQFKCNSDYV PVCGSNGESY QNECYLRQAA 130 140 150 160 170 180 CKQQSEILVV SEGSCATDTG SGSGDGVHEG SGETSQKETS TCDICQFGAE CDEDAEDVWC 190 200 210 220 230 240 VCNIDCSQTN FNPLCASDGK SYDNACQIKE ASCQKQEKIE VMSLGRCQDN TTTTTKSEDG 250 260 270 280 290 300 HYARTDYAEN ANKLEESARE HHIPCPEHYN GFCMHGKCEH SINMQEPSCR CDAGYTGQHC 310 320 330 340 350 360 EKKDYSVLYV VPGPVRFQYV LIAAVIGTIQ IAVICVVVLC ITRKCPRSNR IHRQKQNTGH 370 YSSDNTTRAS TRLI
	Isoform 2 [UniParc]. Checksum: F5F7B4619E1CCFCC	FASTA	374	41,603
	10 20 30 40 50 60 MVLWESPRQC SSWTLCEGFC WLLLLPVTLL IIARPVKLAA FPTSLSDCQT PTGWNCSGYD 70 80 90 100 110 120 DRENDLFLCD TNTCKFDGEC LRIGDTVTCV CQFKCNSDYV PVCGSNGESY QNECYLRQAA 130 140 150 160 170 180 CKQQSEILVV SEGSCATDTG SGSGDGEKFS KVMLILKPCT ASSLEETNSK EIILCNPSNT 190 200 210 220 230 240 HLLKKNENAN LTCWSEPFLQ AYGLMRTWNS RQREAAKGIT ETRFLPPPSA YCLGIFIGAN 250 260 270 280 290 300 VKINSEALFR QWTVLKGTIP VPIPCPEHYN GFCMHGKCEH SINMQEPSCR CDAGYTGQHC 310 320 330 340 350 360 EKKDYSVLYV VPGPVRFQYV LIAAVIGTIQ IAVICVVVLC ITRKCPRSNR IHRQKQNTGH 370 YSSDNTTRAS TRLI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of TMEFF2, a novel survival factor for hippocampal and mesencephalic neurons." Horie M., Mitsumoto Y., Kyushiki H., Kanemoto N., Watanabe A., Taniguchi Y., Nishino N., Okamoto T., Kondo M., Mori T., Noguchi K., Nakamura Y., Takahashi E., Tanigami A. Genomics 67:146-152(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY. Tissue: Brain.
[2]	"Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method." Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Head, Olfactory bulb and Skin.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Mammary gland.
[5]	"A novel epidermal growth factor-like molecule containing two follistatin modules stimulates tyrosine phosphorylation of erbB-4 in MKN28 gastric cancer cells." Uchida T., Wada K., Akamatsu T., Yonezawa M., Noguchi H., Mizoguchi A., Kasuga M., Sakamoto C. Biochem. Biophys. Res. Commun. 266:593-602(1999) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB017270 mRNA. Translation: BAA87898.1. AB041565 mRNA. Translation: BAA95049.1. Frameshift. AK029307 mRNA. Translation: BAC26385.1. AK030053 mRNA. Translation: BAC26760.1. AK076254 mRNA. Translation: BAC36276.1. AK135038 mRNA. Translation: BAE22393.1. BC034850 mRNA. Translation: AAH34850.1.
IPI	IPI00407251. IPI00468627.
RefSeq	NP_062764.1. NM_019790.4.
UniGene	Mm.245154. Mm.445120.
3D structure databases
ProteinModelPortal	Q9QYM9.
SMR	Q9QYM9. Positions 89-228, 272-304.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9QYM9. 1 interaction.
Protein family/group databases
MEROPS	I01.969.
PTM databases
PhosphoSite	Q9QYM9.
Proteomic databases
PaxDb	Q9QYM9.
PRIDE	Q9QYM9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000081851; ENSMUSP00000080533; ENSMUSG00000026109. ENSMUST00000114565; ENSMUSP00000110212; ENSMUSG00000026109.
GeneID	56363.
KEGG	mmu:56363.
UCSC	uc007axj.1. mouse.
Organism-specific databases
CTD	23671.
MGI	MGI:1861735. Tmeff2.
Phylogenomic databases
eggNOG	NOG245393.
GeneTree	ENSGT00530000062996.
HOGENOM	HOG000154541.
HOVERGEN	HBG053816.
InParanoid	Q9QYM9.
OMA	CPRTNRI.
OrthoDB	EOG43BMNZ.
Gene expression databases
Bgee	Q9QYM9.
Genevestigator	Q9QYM9.
GermOnline	ENSMUSG00000026109. Mus musculus.
Family and domain databases
InterPro	IPR000742. EG-like_dom. IPR013032. EGF-like_CS. IPR002350. Kazal_dom. [Graphical view]
Pfam	PF12661. hEGF. 1 hit. PF07648. Kazal_2. 2 hits. [Graphical view]
SMART	SM00181. EGF. 1 hit. SM00280. KAZAL. 2 hits. [Graphical view]
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. 1 hit. PS51465. KAZAL_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	312394.
SOURCE	Search...

Entry information

Entry name

TEFF2_MOUSE

Accession

Primary (citable) accession number: Q9QYM9
Secondary accession number(s): Q3UY20, Q8CDH1, Q9JJE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program