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Protein

DNA repair endonuclease XPF

Gene

ERCC4

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link (By similarity).By similarity

Cofactori

Mg2+By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, Endonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandMagnesium

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair endonuclease XPF (EC:3.1.-.-)
Alternative name(s):
DNA excision repair protein ERCC-4
Gene namesi
Name:ERCC4
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988521 – 913DNA repair endonuclease XPFAdd BLAST913

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei289N6-acetyllysineBy similarity1
Modified residuei518PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Interactioni

Subunit structurei

Heterodimer composed of ERCC1 and XPF/ERCC4. Interacts with SLX4/BTBD12; this interaction is direct and links the ERCC1-XPF/ERCC1 complex to SLX4, which may coordinate the action of the structure-specific endonuclease during DNA repair (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

CORUMiQ9QYM7.

Structurei

3D structure databases

ProteinModelPortaliQ9QYM7.
SMRiQ9QYM7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini680 – 760ERCC4Add BLAST81

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 454Helicase-likeBy similarityAdd BLAST454
Regioni233 – 254Leucine-zipper 1Add BLAST22
Regioni270 – 298Leucine-zipper 2Add BLAST29
Regioni655 – 810NucleaseBy similarityAdd BLAST156
Regioni834 – 902HhH2, dimerization with ERCC1By similarityAdd BLAST69

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi483 – 488Nuclear localization signalSequence analysis6

Sequence similaritiesi

Belongs to the XPF family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG051500.
KOiK10848.

Family and domain databases

InterProiView protein in InterPro
IPR006166. ERCC4_domain.
IPR011335. Restrct_endonuc-II-like.
IPR010994. RuvA_2-like.
IPR006167. XPF.
PfamiView protein in Pfam
PF02732. ERCC4. 1 hit.
SMARTiView protein in SMART
SM00891. ERCC4. 1 hit.
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF52980. SSF52980. 1 hit.
TIGRFAMsiTIGR00596. rad1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9QYM7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRGISAVRK AMAPLLEYER QLVLELLDSD GLVVCARGLG ADRLLYHFLR
60 70 80 90 100
LHCHPACLVL VLNTQPAEEE YFINQLKIEG VEHLPRRVTN EITSNSRYEV
110 120 130 140 150
YTQGGIIFAT SRILVVDFLT DRIPSDLITG ILVYRAHRII ESCQEAFILR
160 170 180 190 200
LFRQKNKRGF IKAFTDNAVA FDTGFCHVER VMRNLFVRKL YLWPRFHVAV
210 220 230 240 250
NSFLEQHKPE VVEIHVSMTP AMLSIQTAIL DILNACLKEL KCHNPSLEVE
260 270 280 290 300
DLSLENALGK PFDKTIRHYL DPLWHQLGAK TKSLVQDLKI LRTLLQYLSQ
310 320 330 340 350
YDCVTFLNLL ESLRATEKVF GQNSGWLFLD ASTSMFVNAR ARVYRVPDVK
360 370 380 390 400
LNKKAKMSES AEGQETKKEL VLESNPKWEA LSEVLKEIEA ENKESEALGG
410 420 430 440 450
PGQVLICASD DRTCCQLRDY LTAGAEAFLL RLYRKTFEKD SKAEEVWVNL
460 470 480 490 500
RKGDGPKRTM KSDKRPKDTK NKERASTKKG APKRKKRELT LTQVMGTAEE
510 520 530 540 550
PPEEGAAEED QQRQATSSPE GCGGEIQHEA FDLNLSSDSA YGILKEPLTI
560 570 580 590 600
IHPLVGCSDP YALTRVLHEV EPRYVVLYDA ELTFVRQLEI YRASRPGKPL
610 620 630 640 650
RVYFLIYGGS TEEQRYLTAL RKEKEAFEKL IREKASMVVP EEREGRDETN
660 670 680 690 700
LDLARGTVST DAPADTRKAG GQEHNGTQPS IVVDMREFRS ELPSLIHRRG
710 720 730 740 750
IDIEPVTLEV GDYILTPELC VERKSVSDLI GSLNSGRLYS QCLAMSRYYR
760 770 780 790 800
RPVLLIEFDA GKPFSLAPRG SFFQEMSSSD VSSKLTLLTL HFPRLRLLWC
810 820 830 840 850
PSPHATAELF EELKQNKPQP DAATAMAITA DSETLPESDK YNPGPQDFVL
860 870 880 890 900
KMPGINAKNC HSLMNHVKNI AELASLSQER LTSILGHAGN AKQLYDFLHT
910
AYADVVSGGR VRK
Length:913
Mass (Da):103,187
Last modified:May 5, 2009 - v3
Checksum:iD1C0DFF990A0F0C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017635 mRNA. Translation: BAA89229.1.
RefSeqiNP_001230961.1. NM_001244032.1.

Genome annotation databases

EnsembliENSCGRT00001004392; ENSCGRP00001003112; ENSCGRG00001003669.
GeneIDi100689044.
KEGGicge:100689044.

Similar proteinsi

Entry informationi

Entry nameiXPF_CRIGR
AccessioniPrimary (citable) accession number: Q9QYM7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 5, 2009
Last modified: October 25, 2017
This is version 85 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families