Reviewed, UniProtKB/Swiss-Prot Q9QYM2 (PARG_RAT)

Names and origin

Protein names

Recommended name:
    Poly(ADP-ribose) glycohydrolase
    EC=3.2.1.143

Gene names

Name:

Parg

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	972 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by poly(ADP-ribose) glycohydrolase. Poly(ADP-ribose) metabolism may be required for maintenance of the normal function of neuronal cells.
Catalytic activity	Hydrolyzes poly(ADP-ribose) at glycosidic (1''-2') linkage of ribose-ribose bond to produce free ADP-ribose.
Subcellular location	Nucleus By similarity.
Tissue specificity	Ubiquitously expressed. Ref.1
Sequence similarities	Belongs to the poly(ADP-ribose) glycohydrolase family.

Ontologies

Keywords
Cellular component	Nucleus
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	chromatin Inferred from direct assay. Source: RGD nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chromatin binding Inferred from direct assay. Source: RGD poly(ADP-ribose) glycohydrolase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 972	972	Poly(ADP-ribose) glycohydrolase		PRO_0000066604
Regions
	Motif	10 – 16	7	Nuclear localization signal By similarity
Amino acid modifications
	Modified residue	135	1	Phosphoserine By similarity
	Modified residue	137	1	Phosphothreonine By similarity
	Modified residue	195	1	Phosphoserine By similarity
	Modified residue	197	1	Phosphothreonine By similarity
	Modified residue	256	1	Phosphoserine By similarity
	Modified residue	259	1	Phosphoserine By similarity
	Modified residue	311	1	Phosphoserine By similarity
	Modified residue	499	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QYM2-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 3DED7885684F59E9	FASTA	972	109,003
	10 20 30 40 50 60 MSAGPGCEPC TKRPRWGAAG TSAPTASDSR SFPGRQKRVL DPKDAPVQFR VPPSSSACVS 70 80 90 100 110 120 GRAGPHRGSV TSFVFKQKPI TTWMDTKGPK TAESESKENN NTRTDPMMSS VQKDNFYPHK 130 140 150 160 170 180 VEKLGNVPQL NLDKSPTEKS TPYLNQQQTA GVCKWHSAGE RAEQLSASEP SAVTQAPKQL 190 200 210 220 230 240 SNANIDQSPP TDGHSDTDHE EDRDNQQFLT PVKLANAKQT VGDGQARSNC KCSASCQCGQ 250 260 270 280 290 300 DCAGCQREEA DVIPESPLSD VGAEDIGTGS KNDNKLTGQE SGLGDSPPFE KESEPESPMD 310 320 330 340 350 360 VDNSKTSCQD SEADEEASPV FDEQDDQDDR SSQTANKLSS RQAREVDGDL RKRYLTKGSE 370 380 390 400 410 420 IRLHFQFEGG SNAGTSDLNA KPSGNSSSLN VDGRSSKQHG KRDSKITDHF VRIPKSEDKR 430 440 450 460 470 480 KEQCEVRHQR AERKIPKYVP PNLPPDKKWL GTPIEEMRKM PRCGVRLPLL RPSASHTVTV 490 500 510 520 530 540 RVDLLRAGEV PKPFPTHYKD LWDNKHVKMP CSEQNLYPVE DENGERTAGS RWELIQTALL 550 560 570 580 590 600 NKFTRPQNLK DAILKYNVAY SKKWDFTALV DFWDKVLEEA EAQHLYQSIL PDMVKIALCL 610 620 630 640 650 660 PNICTQPIPL LKQKMNHSVT MSQEQIASLL ANAFFCTFPR RNAKMKSEYS SYPDINFNRL 670 680 690 700 710 720 FEGRSSRKPE KLKTLFCYFR RVTEKKPTGL VTFTRQSLED FPEWERCDKP LTRLHVTYEG 730 740 750 760 770 780 TIEGNGRGML QVDFANRFVG GGVTGAGLVQ EEIRFLINPE LIVSRLFTEV LDHNECLIIT 790 800 810 820 830 840 GTEQYSEYTG YAETYRWARS HEDGSEKDDW QRCCTEIVAI DALHFRRYLD QFVPEKVRRE 850 860 870 880 890 900 LNKAYCGFLR PGVPPENLSA VATGNWGCGA FGGDARLKAL IQLLAAAAAE RDVVYFTFGD 910 920 930 940 950 960 SELMRDIYSM HTFLTERKLN VGKVYRLLLR YYREECRDCS SPGPDTKLYP FIYHAAESSA 970 ETSDQPGQRT GT

References

[1]

"Isolation and cloning of rat poly(ADP-ribose) glycohydrolase: presence of a potential nuclear export signal conserved in mammalian orthologs." Shimokawa T., Masutani M., Nagasawa S., Nozaki T., Ikota N., Aoki Y., Nakagama H., Sugimura T. J. Biochem. 126:748-755(1999) [PubMed: 10502684] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 543-550; 556-562 AND 948-957, TISSUE SPECIFICITY. Strain: Buffalo. Tissue: Colon.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB019366 mRNA. Translation: BAA87901.1.
IPI	IPI00214269.
RefSeq	NP_112629.1.
UniGene	Rn.13634
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q9QYM2.
Genome annotation databases
Ensembl	ENSRNOT00000027071; ENSRNOP00000027071; ENSRNOG00000019978; Rattus norvegicus. [Genome view]
GeneID	83507.
KEGG	rno:83507.
UCSC	NM_031339. rat.
Organism-specific databases
CTD	83507.
RGD	620387. Parg.
Phylogenomic databases
HOVERGEN	Q9QYM2.
Enzyme and pathway databases
BRENDA	3.2.1.143. 248.
Gene expression databases
ArrayExpress	Q9QYM2.
Genevestigator	Q9QYM2.
GermOnline	ENSRNOG00000019978. Rattus norvegicus.
Family and domain databases
InterPro	IPR007724. Poly_GlycHdrlase. [Graphical view]
PANTHER	PTHR12837. Poly_glchydro. 1 hit.
Pfam	PF05028. PARG_cat. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615942.

Entry information

Entry name

PARG_RAT

Accession

Primary (citable) accession number: Q9QYM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2004
Last sequence update:	May 1, 2000
Last modified:	October 13, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)