Q9QYK9 (KCC1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcium/calmodulin-dependent protein kinase type 1B
EC=2.7.11.17

Alternative name(s):
CaM kinase I beta
Short name=CaM kinase IB
Short name=CaM-KI beta
Short name=CaMKI-beta
Pregnancy up-regulated non-ubiquitously-expressed CaM kinase homolog

Gene names

Name:

Pnck

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Activated by Ca2+/calmodulin By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.1.
Tissue specificity	Expressed at highest levels in adult brain, and expressed in embryo. In the adult brain detected at high levels in the anterior olfactory nuclei, piriform cortex, septal nuclei, bed nuclei of the stria terminalis, hippocampal pyramidal cells, dentate granule cells, amygdala, hypothalamic nuclei, parabrachial nucleus, and nucleus of the solitary tract. Expressed at lower levels in adult ovary and heart and at very low levels in testis, lung and muscle. Ref.1 Ref.2
Developmental stage	During embryogenesis detected at E10 and expression gradually increases thereafter. Expressed mainly in the nervous system, including brain, spinal cord, trigeminal ganglion, and retina. Within the CNS detected in the mantle zone, but not in the ventricular zone. Detected at postnatal day 23 with highest levels in mesencephalon. Also expressed in developing bone and gut. Ref.1 Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAH51996.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Calcium Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	calcium- and calmodulin-dependent protein kinase complex Traceable author statement. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Calcium/calmodulin-dependent protein kinase type 1B		PRO_0000086080
Regions
	Domain	15 – 270	256	Protein kinase
	Nucleotide binding	21 – 29	9	ATP By similarity
	Region	290 – 311	22	Calmodulin-binding By similarity
Sites
	Active site	136	1	Proton acceptor By similarity
	Binding site	44	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9QYK9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 1B4A28D36E7A936E	FASTA	343	38,519
	10 20 30 40 50 60 MLLLKKQTED ISSVYEIREK LGSGAFSEVM LAQERGSAHL VALKCIPKKA LRGKEALVEN 70 80 90 100 110 120 EIAVLRRISH PNIVALEDVH ESPSHLYLAM ELVTGGELFD RIMERGSYTE KDASHLVGQV 130 140 150 160 170 180 LGAVSYLHSL GIVHRDLKPE NLLYATPFED SKIMVSDFGL SKIQAGNMLG TACGTPGYVA 190 200 210 220 230 240 PELLEQKPYG KAVDVWALGV ISYILLCGYP PFYDESDPEL FSQILRASYE FDSPFWDDIS 250 260 270 280 290 300 ESAKDFIRHL LERDPQKRFT CQQALQHLWI SGDAAFDRDI LGSVSEQIQK NFARTHWKRA 310 320 330 340 FNATSFLRHI RKLGQSPEGE EASRQCMTRH SHPGLGTSQS PKW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Distribution and intracellular localization of a mouse homologue of Ca2+/calmodulin-dependent protein kinase Ibeta2 in the nervous system." Ueda T., Sakagami H., Abe K., Oishi I., Maruo A., Kondo H., Terashima T., Ichihashi M., Yamamura H., Minami Y. J. Neurochem. 73:2119-2129(1999) [PubMed: 10537072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[2]	"Cloning, characterization, and chromosomal localization of Pnck, a Ca2+/calmodulin-pependent protein kinase." Gardner H.P., Rajan J.V., Ha S.I., Copeland N.G., Gilbert D.J., Jenkins N.A., Marquis S.T., Chodosh L.A. Genomics 63:279-288(2000) [PubMed: 10673339] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Brain and Colon.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB023027 mRNA. Translation: BAA87926.1. AF181984 mRNA. Translation: AAF29157.1. BC051996 mRNA. Translation: AAH51996.1. Different initiation. BC055891 mRNA. Translation: AAH55891.1.
IPI	IPI00136288.
RefSeq	NP_001186280.1. NM_001199351.1. NP_001186281.1. NM_001199352.1. NP_036170.1. NM_012040.3.
UniGene	Mm.89564.
3D structure databases
ProteinModelPortal	Q9QYK9.
SMR	Q9QYK9. Positions 8-294.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9QYK9.
PTM databases
PhosphoSite	Q9QYK9.
Proteomic databases
PRIDE	Q9QYK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000002087; ENSMUSP00000002087; ENSMUSG00000002012. ENSMUST00000114472; ENSMUSP00000110116; ENSMUSG00000002012. ENSMUST00000114473; ENSMUSP00000110117; ENSMUSG00000002012.
GeneID	93843.
KEGG	mmu:93843.
Organism-specific databases
CTD	139728.
MGI	MGI:1347357. Pnck.
Phylogenomic databases
GeneTree	ENSGT00600000084207.
HOGENOM	HBG755340.
HOVERGEN	HBG108055.
InParanoid	Q9QYK9.
OMA	IYESPTH.
OrthoDB	EOG4T4CVW.
PhylomeDB	Q9QYK9.
Gene expression databases
ArrayExpress	Q9QYK9.
Bgee	Q9QYK9.
Genevestigator	Q9QYK9.
GermOnline	ENSMUSG00000002012. Mus musculus.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. IPR002290. Ser/Thr_kinase_dom. [Graphical view]
KO	K08795.
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	351709.
SOURCE	Search...

Entry information

Entry name

KCC1B_MOUSE

Accession

Primary (citable) accession number: Q9QYK9
Secondary accession number(s): Q80W07

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	May 1, 2000
Last modified:	November 16, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program