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Protein

Calcium/calmodulin-dependent protein kinase type 1B

Gene

Pnck

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by Ca2+/calmodulin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei44 – 441ATPPROSITE-ProRule annotation
Active sitei136 – 1361Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi21 – 299ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium/calmodulin-dependent protein kinase type 1B (EC:2.7.11.17)
Alternative name(s):
CaM kinase I beta
Short name:
CaM kinase IB
Short name:
CaM-KI beta
Short name:
CaMKI-beta
Pregnancy up-regulated non-ubiquitously-expressed CaM kinase homolog
Gene namesi
Name:Pnck
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1347357. Pnck.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 343343Calcium/calmodulin-dependent protein kinase type 1BPRO_0000086080Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei338 – 3381PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9QYK9.
PaxDbiQ9QYK9.
PRIDEiQ9QYK9.

PTM databases

iPTMnetiQ9QYK9.
PhosphoSiteiQ9QYK9.

Expressioni

Tissue specificityi

Expressed at highest levels in adult brain, and expressed in embryo. In the adult brain detected at high levels in the anterior olfactory nuclei, piriform cortex, septal nuclei, bed nuclei of the stria terminalis, hippocampal pyramidal cells, dentate granule cells, amygdala, hypothalamic nuclei, parabrachial nucleus, and nucleus of the solitary tract. Expressed at lower levels in adult ovary and heart and at very low levels in testis, lung and muscle.2 Publications

Developmental stagei

During embryogenesis detected at E10 and expression gradually increases thereafter. Expressed mainly in the nervous system, including brain, spinal cord, trigeminal ganglion, and retina. Within the CNS detected in the mantle zone, but not in the ventricular zone. Detected at postnatal day 23 with highest levels in mesencephalon. Also expressed in developing bone and gut.2 Publications

Gene expression databases

BgeeiQ9QYK9.
ExpressionAtlasiQ9QYK9. baseline and differential.
GenevisibleiQ9QYK9. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002087.

Structurei

3D structure databases

ProteinModelPortaliQ9QYK9.
SMRiQ9QYK9. Positions 6-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 270256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni290 – 31122Calmodulin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiQ9QYK9.
KOiK08795.
OMAiNFARNHW.
OrthoDBiEOG7WHH9K.
PhylomeDBiQ9QYK9.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QYK9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLKKQTED ISSVYEIREK LGSGAFSEVM LAQERGSAHL VALKCIPKKA
60 70 80 90 100
LRGKEALVEN EIAVLRRISH PNIVALEDVH ESPSHLYLAM ELVTGGELFD
110 120 130 140 150
RIMERGSYTE KDASHLVGQV LGAVSYLHSL GIVHRDLKPE NLLYATPFED
160 170 180 190 200
SKIMVSDFGL SKIQAGNMLG TACGTPGYVA PELLEQKPYG KAVDVWALGV
210 220 230 240 250
ISYILLCGYP PFYDESDPEL FSQILRASYE FDSPFWDDIS ESAKDFIRHL
260 270 280 290 300
LERDPQKRFT CQQALQHLWI SGDAAFDRDI LGSVSEQIQK NFARTHWKRA
310 320 330 340
FNATSFLRHI RKLGQSPEGE EASRQCMTRH SHPGLGTSQS PKW
Length:343
Mass (Da):38,519
Last modified:May 1, 2000 - v1
Checksum:i1B4A28D36E7A936E
GO

Sequence cautioni

The sequence AAH51996.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023027 mRNA. Translation: BAA87926.1.
AF181984 mRNA. Translation: AAF29157.1.
BC051996 mRNA. Translation: AAH51996.1. Different initiation.
BC055891 mRNA. Translation: AAH55891.1.
CCDSiCCDS30207.1.
RefSeqiNP_001186280.1. NM_001199351.1.
NP_001186281.1. NM_001199352.1.
NP_036170.1. NM_012040.3.
XP_006528407.1. XM_006528344.2.
XP_011245994.1. XM_011247692.1.
UniGeneiMm.89564.

Genome annotation databases

EnsembliENSMUST00000002087; ENSMUSP00000002087; ENSMUSG00000002012.
ENSMUST00000114472; ENSMUSP00000110116; ENSMUSG00000002012.
ENSMUST00000114473; ENSMUSP00000110117; ENSMUSG00000002012.
GeneIDi93843.
KEGGimmu:93843.
UCSCiuc009tmc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023027 mRNA. Translation: BAA87926.1.
AF181984 mRNA. Translation: AAF29157.1.
BC051996 mRNA. Translation: AAH51996.1. Different initiation.
BC055891 mRNA. Translation: AAH55891.1.
CCDSiCCDS30207.1.
RefSeqiNP_001186280.1. NM_001199351.1.
NP_001186281.1. NM_001199352.1.
NP_036170.1. NM_012040.3.
XP_006528407.1. XM_006528344.2.
XP_011245994.1. XM_011247692.1.
UniGeneiMm.89564.

3D structure databases

ProteinModelPortaliQ9QYK9.
SMRiQ9QYK9. Positions 6-293.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000002087.

PTM databases

iPTMnetiQ9QYK9.
PhosphoSiteiQ9QYK9.

Proteomic databases

MaxQBiQ9QYK9.
PaxDbiQ9QYK9.
PRIDEiQ9QYK9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002087; ENSMUSP00000002087; ENSMUSG00000002012.
ENSMUST00000114472; ENSMUSP00000110116; ENSMUSG00000002012.
ENSMUST00000114473; ENSMUSP00000110117; ENSMUSG00000002012.
GeneIDi93843.
KEGGimmu:93843.
UCSCiuc009tmc.2. mouse.

Organism-specific databases

CTDi139728.
MGIiMGI:1347357. Pnck.

Phylogenomic databases

eggNOGiKOG0032. Eukaryota.
ENOG410XRMJ. LUCA.
GeneTreeiENSGT00760000118944.
HOGENOMiHOG000233016.
HOVERGENiHBG108055.
InParanoidiQ9QYK9.
KOiK08795.
OMAiNFARNHW.
OrthoDBiEOG7WHH9K.
PhylomeDBiQ9QYK9.
TreeFamiTF314166.

Miscellaneous databases

PROiQ9QYK9.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QYK9.
ExpressionAtlasiQ9QYK9. baseline and differential.
GenevisibleiQ9QYK9. MM.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Distribution and intracellular localization of a mouse homologue of Ca2+/calmodulin-dependent protein kinase Ibeta2 in the nervous system."
    Ueda T., Sakagami H., Abe K., Oishi I., Maruo A., Kondo H., Terashima T., Ichihashi M., Yamamura H., Minami Y.
    J. Neurochem. 73:2119-2129(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Cloning, characterization, and chromosomal localization of Pnck, a Ca2+/calmodulin-pependent protein kinase."
    Gardner H.P., Rajan J.V., Ha S.I., Copeland N.G., Gilbert D.J., Jenkins N.A., Marquis S.T., Chodosh L.A.
    Genomics 63:279-288(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Brain.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Brain and Colon.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-338, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiKCC1B_MOUSE
AccessioniPrimary (citable) accession number: Q9QYK9
Secondary accession number(s): Q80W07
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.