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Protein

RING finger protein 11

Gene

Rnf11

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. Promotes the association of TNFAIP3 to RIPK1 after TNF stimulation. TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Recruits STAMBP to the E3 ubiquitin-ligase SMURF2 for ubiquitination, leading to its degradation by the 26S proteasome.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri99 – 14042RING-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
RING finger protein 11
Alternative name(s):
NEDD4 WW domain-binding protein 2
Sid 1669
Gene namesi
Name:Rnf11
Synonyms:N4wbp2, Sid1669
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1352759. Rnf11.

Subcellular locationi

GO - Cellular componenti

  • early endosome Source: UniProtKB-SubCell
  • extracellular exosome Source: MGI
  • nucleus Source: UniProtKB-SubCell
  • recycling endosome Source: UniProtKB-SubCell
  • ubiquitin ligase complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi40 – 401Y → A: Abolishes interaction with NEDD4. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 154153RING finger protein 11PRO_0000056051Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi4 – 41S-palmitoyl cysteineBy similarity
Modified residuei14 – 141PhosphoserineCombined sources
Modified residuei25 – 251PhosphoserineCombined sources
Modified residuei135 – 1351Phosphothreonine; by PKB/AKT1By similarity

Post-translational modificationi

Ubiquitinated in the presence of ITCH, SMURF2 and UBE2D1, as well as WWP1.By similarity
Phosphorylation by PKB/AKT1 may accelerate degradation by the proteasome.By similarity
Acylation at both Gly-2 and Cys-4 is required for proper localization to the endosomes.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9QYK7.
PaxDbiQ9QYK7.
PeptideAtlasiQ9QYK7.
PRIDEiQ9QYK7.

PTM databases

iPTMnetiQ9QYK7.
PhosphoSiteiQ9QYK7.
SwissPalmiQ9QYK7.

Expressioni

Gene expression databases

BgeeiQ9QYK7.
CleanExiMM_RNF11.
ExpressionAtlasiQ9QYK7. baseline and differential.
GenevisibleiQ9QYK7. MM.

Interactioni

Subunit structurei

Interacts (when phosphorylated) with 14-3-3. Interacts with the E3 ubiquitin-ligases NEDD4, ITCH, SMURF2 and WWP1 (By similarity). Also interacts with the E2 ubiquitin-conjugating enzymes UBE2D1 and UBE2N, but neither with CDC34, nor with UBE2L3. Interacts with ZNF350, EPS15 and STAMBP (By similarity). After TNF stimulation, interacts with TAX1BP1, TNFAIP3 and RIPK1; these interaction are transient and they are lost after 1 hour of stimulation with TNF. Interacts with GGA1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Nedd4P469354EBI-4405826,EBI-773516

Protein-protein interaction databases

BioGridi205930. 9 interactions.
IntActiQ9QYK7. 8 interactions.
MINTiMINT-147742.
STRINGi10090.ENSMUSP00000030284.

Structurei

3D structure databases

ProteinModelPortaliQ9QYK7.
SMRiQ9QYK7. Positions 98-139.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 404WW-binding

Domaini

The WW-binding motif mediates interaction with NEDD4.1 Publication

Sequence similaritiesi

Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri99 – 14042RING-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0800. Eukaryota.
ENOG41121N2. LUCA.
GeneTreeiENSGT00730000110988.
HOGENOMiHOG000007448.
HOVERGENiHBG058444.
InParanoidiQ9QYK7.
KOiK11980.
OMAiHLDCIDN.
OrthoDBiEOG7H4DVW.
PhylomeDBiQ9QYK7.
TreeFamiTF318022.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QYK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNCLKSPTS DDISLLHESQ SDRASFGEGT EPDQEPPPPY QEQVPVPIYH
60 70 80 90 100
PTPSQTRLAT QLTEEEQIRI AQRIGLIQHL PKGVYDPGRD GSEKKIRECV
110 120 130 140 150
ICMMDFVYGD PIRFLPCMHI YHLDCIDDWL MRSFTCPSCM EPVDAALLSS

YETN
Length:154
Mass (Da):17,458
Last modified:May 1, 2000 - v1
Checksum:iEF192AB0C2D4BF87
GO

Sequence cautioni

The sequence AAG44245.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024427 mRNA. Translation: BAA84682.1.
AF220206 mRNA. Translation: AAG44245.1. Different initiation.
AL669905 Genomic DNA. Translation: CAM13662.1.
AL669905 Genomic DNA. Translation: CAM13663.1.
CH466527 Genomic DNA. Translation: EDL30703.1.
CH466527 Genomic DNA. Translation: EDL30704.1.
CH466527 Genomic DNA. Translation: EDL30705.1.
BC010299 mRNA. Translation: AAH10299.1.
BC028255 mRNA. Translation: AAH28255.1.
CCDSiCCDS18464.1.
RefSeqiNP_038904.1. NM_013876.3.
XP_011238851.1. XM_011240549.1.
UniGeneiMm.392580.
Mm.456388.

Genome annotation databases

EnsembliENSMUST00000030284; ENSMUSP00000030284; ENSMUSG00000028557.
ENSMUST00000064167; ENSMUSP00000063798; ENSMUSG00000028557.
GeneIDi29864.
KEGGimmu:29864.
UCSCiuc008ucm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB024427 mRNA. Translation: BAA84682.1.
AF220206 mRNA. Translation: AAG44245.1. Different initiation.
AL669905 Genomic DNA. Translation: CAM13662.1.
AL669905 Genomic DNA. Translation: CAM13663.1.
CH466527 Genomic DNA. Translation: EDL30703.1.
CH466527 Genomic DNA. Translation: EDL30704.1.
CH466527 Genomic DNA. Translation: EDL30705.1.
BC010299 mRNA. Translation: AAH10299.1.
BC028255 mRNA. Translation: AAH28255.1.
CCDSiCCDS18464.1.
RefSeqiNP_038904.1. NM_013876.3.
XP_011238851.1. XM_011240549.1.
UniGeneiMm.392580.
Mm.456388.

3D structure databases

ProteinModelPortaliQ9QYK7.
SMRiQ9QYK7. Positions 98-139.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205930. 9 interactions.
IntActiQ9QYK7. 8 interactions.
MINTiMINT-147742.
STRINGi10090.ENSMUSP00000030284.

PTM databases

iPTMnetiQ9QYK7.
PhosphoSiteiQ9QYK7.
SwissPalmiQ9QYK7.

Proteomic databases

MaxQBiQ9QYK7.
PaxDbiQ9QYK7.
PeptideAtlasiQ9QYK7.
PRIDEiQ9QYK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030284; ENSMUSP00000030284; ENSMUSG00000028557.
ENSMUST00000064167; ENSMUSP00000063798; ENSMUSG00000028557.
GeneIDi29864.
KEGGimmu:29864.
UCSCiuc008ucm.1. mouse.

Organism-specific databases

CTDi26994.
MGIiMGI:1352759. Rnf11.

Phylogenomic databases

eggNOGiKOG0800. Eukaryota.
ENOG41121N2. LUCA.
GeneTreeiENSGT00730000110988.
HOGENOMiHOG000007448.
HOVERGENiHBG058444.
InParanoidiQ9QYK7.
KOiK11980.
OMAiHLDCIDN.
OrthoDBiEOG7H4DVW.
PhylomeDBiQ9QYK7.
TreeFamiTF318022.

Miscellaneous databases

PROiQ9QYK7.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QYK7.
CleanExiMM_RNF11.
ExpressionAtlasiQ9QYK7. baseline and differential.
GenevisibleiQ9QYK7. MM.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiIPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF13639. zf-RING_2. 1 hit.
[Graphical view]
SMARTiSM00184. RING. 1 hit.
[Graphical view]
PROSITEiPS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression profile of mouse and human genes, Rnf11/RNF11, encoding a novel RING-H2 finger protein."
    Seki N., Hattori A., Hayashi A., Kozuma S., Sasaki M., Suzuki Y., Sugano S., Muramatsu M., Saito T.
    Biochim. Biophys. Acta 1489:421-427(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4."
    Jolliffe C.N., Harvey K.F., Haines B.P., Parasivam G., Kumar S.
    Biochem. J. 351:557-565(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DOMAIN, INTERACTION WITH NEDD4, MUTAGENESIS OF TYR-40.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  6. "The ubiquitin-editing enzyme A20 requires RNF11 to downregulate NF-kappaB signalling."
    Shembade N., Parvatiyar K., Harhaj N.S., Harhaj E.W.
    EMBO J. 28:513-522(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TAX1BP1; TNFAIP3 AND RIPK1.
  7. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14 AND SER-25, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Kidney, Liver, Spleen and Testis.

Entry informationi

Entry nameiRNF11_MOUSE
AccessioniPrimary (citable) accession number: Q9QYK7
Secondary accession number(s): B1AU36, B1AU37, Q9EQI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.