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Protein

Heparan-sulfate 6-O-sulfotransferase 1

Gene

Hs6st1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Critical for normal neuronal development where it may play a role in neuron branching. May also play a role in limb development. May prefer iduronic acid.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.

Kineticsi

  1. KM=10 µM for CDSNS-heparin1 Publication
  2. KM=100 µM for NS-heparosan1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei140SubstrateBy similarity1
    Binding sitei145SubstrateBy similarity1
    Active sitei150Proton acceptorBy similarity1
    Binding sitei150SubstrateBy similarity1
    Binding sitei185PAPSBy similarity1
    Binding sitei193PAPSBy similarity1
    Binding sitei197SubstrateBy similarity1
    Binding sitei204SubstrateBy similarity1

    GO - Molecular functioni

    • heparan sulfate 6-O-sulfotransferase activity Source: MGI

    GO - Biological processi

    • angiogenesis Source: MGI
    • heparan sulfate proteoglycan biosynthetic process Source: MGI
    • heparan sulfate proteoglycan biosynthetic process, enzymatic modification Source: MGI
    • labyrinthine layer blood vessel development Source: MGI
    • lung alveolus development Source: MGI
    • neuron development Source: UniProtKB

    Keywordsi

    Molecular functionTransferase

    Enzyme and pathway databases

    ReactomeiR-MMU-2022928. HS-GAG biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heparan-sulfate 6-O-sulfotransferase 1 (EC:2.8.2.-)
    Short name:
    HS6ST-1
    Short name:
    mHS6ST-1
    Gene namesi
    Name:Hs6st1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 1

    Organism-specific databases

    MGIiMGI:1354958. Hs6st1.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 19CytoplasmicSequence analysisAdd BLAST19
    Transmembranei20 – 37Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST18
    Topological domaini38 – 411LumenalSequence analysisAdd BLAST374

    GO - Cellular componenti

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    According to PubMed:17405882 most mice die between embryonic day 15.5 and the perinatal stage. Those that survive are considerably smaller than their wild-type littermates and exhibit development abnormalities including a reduction in the number of fetal microvessels in the labyrinthine zone of the placenta. However, according to PubMed:18196599, pups are viable and grossly normal at birth. During early adulthood, however, mice fail to thrive and exhibit growth retardation, bodyweight loss, enlargement of airspaces in the lung and, in some cases, lethality.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001908021 – 411Heparan-sulfate 6-O-sulfotransferase 1Add BLAST411

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi264N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi320N-linked (GlcNAc...) asparagineSequence analysis1

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ9QYK5.
    PaxDbiQ9QYK5.
    PeptideAtlasiQ9QYK5.
    PRIDEiQ9QYK5.

    PTM databases

    PhosphoSitePlusiQ9QYK5.

    Expressioni

    Tissue specificityi

    Expressed in fetal brain and liver.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000045216.
    CleanExiMM_HS6ST1.
    GenevisibleiQ9QYK5. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000085499.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QYK5.
    SMRiQ9QYK5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni93 – 101PAPS bindingBy similarity9
    Regioni123 – 124Substrate bindingBy similarity2
    Regioni317 – 319PAPS bindingBy similarity3
    Regioni323 – 324PAPS bindingBy similarity2

    Coiled coil

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Coiled coili352 – 386Sequence analysisAdd BLAST35

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi2 – 5Poly-Arg4

    Sequence similaritiesi

    Belongs to the sulfotransferase 6 family.Curated

    Keywords - Domaini

    Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3955. Eukaryota.
    ENOG410XSW4. LUCA.
    GeneTreeiENSGT00390000013468.
    HOGENOMiHOG000007772.
    HOVERGENiHBG083012.
    InParanoidiQ9QYK5.
    KOiK02514.
    OMAiQNLRPDQ.
    OrthoDBiEOG091G08UD.
    PhylomeDBiQ9QYK5.
    TreeFamiTF312835.

    Family and domain databases

    InterProiView protein in InterPro
    IPR010635. Heparan_SO4-6-sulfoTrfase.
    IPR027417. P-loop_NTPase.
    IPR005331. Sulfotransferase.
    PANTHERiPTHR12812. PTHR12812. 1 hit.
    PfamiView protein in Pfam
    PF03567. Sulfotransfer_2. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9QYK5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRRRRAGGRT MVERASKFVL VVAGSACFML ILYQYAGPGL SLGAPGGRVP
    60 70 80 90 100
    PDDLDLFPTP DPHYEKKYYF PVRELERSLR FDMKGDDVIV FLHIQKTGGT
    110 120 130 140 150
    TFGRHLVQNV RLEVPCDCRP GQKKCTCYRP NRRETWLFSR FSTGWSCGLH
    160 170 180 190 200
    ADWTELTNCV PGVLDRRDPA GLRSPRKFYY ITLLRDPVSR YLSEWRHVQR
    210 220 230 240 250
    GATWKTSLHM CDGRTPTPEE LPPCYEGTDW SGCTLQEFMD CPYNLANNRQ
    260 270 280 290 300
    VRMLADLSLV GCYNLSFIPE SKRAQLLLES AKKNLRGMAF FGLTEFQRKT
    310 320 330 340 350
    QYLFERTFNL KFIRPFMQYN STRAGGVEVD EDTIRHIEEL NDLDMQLYDY
    360 370 380 390 400
    AKDLFQQRYQ YKRQLERREQ RLRNREERLL HRSKEALPRE DPEEPGRVPT
    410
    EDYMSHIIEK W
    Length:411
    Mass (Da):48,302
    Last modified:May 5, 2009 - v4
    Checksum:i8A618806A8A1D6AE
    GO

    Sequence cautioni

    The sequence AAH52316 differs from that shown. Reason: Erroneous initiation.Curated
    The sequence BAA89248 differs from that shown. Reason: Erroneous initiation.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti125C → S in BAE38155 (PubMed:16141072).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB024566 mRNA. Translation: BAA89248.1. Different initiation.
    AK157556 mRNA. Translation: BAE34122.1.
    AK165385 mRNA. Translation: BAE38155.1.
    AK166962 mRNA. Translation: BAE39145.1.
    AK167151 mRNA. Translation: BAE39293.1.
    BC052316 mRNA. Translation: AAH52316.1. Different initiation.
    CCDSiCCDS48237.1.
    RefSeqiNP_056633.2. NM_015818.2.
    UniGeneiMm.213566.

    Genome annotation databases

    EnsembliENSMUST00000088174; ENSMUSP00000085499; ENSMUSG00000045216.
    GeneIDi50785.
    KEGGimmu:50785.
    UCSCiuc007apn.2. mouse.

    Similar proteinsi

    Entry informationi

    Entry nameiH6ST1_MOUSE
    AccessioniPrimary (citable) accession number: Q9QYK5
    Secondary accession number(s): Q3TK52
    , Q3TKK0, Q3TND4, Q3TZT5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
    Last sequence update: May 5, 2009
    Last modified: August 30, 2017
    This is version 107 of the entry and version 4 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families