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Protein

Heparan-sulfate 6-O-sulfotransferase 3

Gene

Hs6st3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.

Kineticsi

  1. KM=20 µM for CDSNS-heparin1 Publication
  2. KM=20 µM for NS-heparosan1 Publication

    GO - Molecular functioni

    • heparan sulfate 6-O-sulfotransferase activity Source: MGI

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heparan-sulfate 6-O-sulfotransferase 3 (EC:2.8.2.-)
    Short name:
    HS6ST-3
    Short name:
    mHS6ST-3
    Gene namesi
    Name:Hs6st3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 14

    Organism-specific databases

    MGIiMGI:1354960. Hs6st3.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 44CytoplasmicSequence analysis
    Transmembranei5 – 2723Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
    BLAST
    Topological domaini28 – 470443LumenalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 470470Heparan-sulfate 6-O-sulfotransferase 3PRO_0000190810Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi116 – 1161N-linked (GlcNAc...)Sequence analysis
    Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence analysis
    Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence analysis
    Glycosylationi323 – 3231N-linked (GlcNAc...)Sequence analysis
    Glycosylationi328 – 3281N-linked (GlcNAc...)Sequence analysis
    Glycosylationi379 – 3791N-linked (GlcNAc...)Sequence analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9QYK4.
    PRIDEiQ9QYK4.

    PTM databases

    iPTMnetiQ9QYK4.
    PhosphoSiteiQ9QYK4.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Gene expression databases

    BgeeiQ9QYK4.
    CleanExiMM_HS6ST3.
    GenevisibleiQ9QYK4. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000070394.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QYK4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni154 – 16075'-phosphosulfate-bindingSequence analysis
    Regioni244 – 25293'-phosphate bindingSequence analysis

    Sequence similaritiesi

    Belongs to the sulfotransferase 6 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3955. Eukaryota.
    ENOG410XSW4. LUCA.
    GeneTreeiENSGT00390000013468.
    HOGENOMiHOG000007772.
    HOVERGENiHBG083012.
    InParanoidiQ9QYK4.
    KOiK08103.
    OMAiSSMNESQ.
    OrthoDBiEOG75MVW3.
    TreeFamiTF312835.

    Family and domain databases

    InterProiIPR010635. Heparan_SO4-6-sulfoTrfase.
    IPR027417. P-loop_NTPase.
    IPR005331. Sulfotransferase.
    [Graphical view]
    PANTHERiPTHR12812. PTHR12812. 2 hits.
    PfamiPF03567. Sulfotransfer_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9QYK4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDERFNKWLL TPVLTLLFVV IMYQYVSPSC TSSCTNFGEQ LRSGEARPPA
    60 70 80 90 100
    VPSPARRAQA PLDEWERRPQ LPPPPRGPPE GSRGVAAPED EDEDPGDPEE
    110 120 130 140 150
    EEEEEEEEPD PEAPENGSLP RFVPRFNFTL KDLTRFVDFN IKGRDVIVFL
    160 170 180 190 200
    HIQKTGGTTF GRHLVKNIRL EQPCSCKAGQ KKCTCHRPGK KETWLFSRFS
    210 220 230 240 250
    TGWSCGLHAD WTELTNCVPA IMEKKDCPRN HSHTRNFYYI TMLRDPVSRY
    260 270 280 290 300
    LSEWKHVQRG ATWKTSLHMC DGRSPTPDEL PTCYPGDDWS GVSLREFMDC
    310 320 330 340 350
    SYNLANNRQV RMLADLSLVG CYNLTFMNES ERNTILLQSA KNNLKNMAFF
    360 370 380 390 400
    GLTEFQRKTQ FLFERTFNLK FISPFTQFNI TRASNVDIND GARQHIEELN
    410 420 430 440 450
    FLDMQLYEYA KDLFQQRYHH TKQLEHQRDR QKRREERRLQ REHRAHRWPK
    460 470
    EDRAMEGTVT EDYNSQVVRW
    Length:470
    Mass (Da):55,037
    Last modified:July 27, 2011 - v2
    Checksum:i195B376684A4328D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161L → F in BAA89249 (PubMed:10644753).Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB024567 mRNA. Translation: BAA89249.1.
    BC118035 mRNA. Translation: AAI18036.1.
    CCDSiCCDS27339.1.
    RefSeqiNP_056635.2. NM_015820.3.
    UniGeneiMm.445777.

    Genome annotation databases

    EnsembliENSMUST00000065904; ENSMUSP00000070394; ENSMUSG00000053465.
    GeneIDi50787.
    KEGGimmu:50787.
    UCSCiuc007uzn.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB024567 mRNA. Translation: BAA89249.1.
    BC118035 mRNA. Translation: AAI18036.1.
    CCDSiCCDS27339.1.
    RefSeqiNP_056635.2. NM_015820.3.
    UniGeneiMm.445777.

    3D structure databases

    ProteinModelPortaliQ9QYK4.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000070394.

    PTM databases

    iPTMnetiQ9QYK4.
    PhosphoSiteiQ9QYK4.

    Proteomic databases

    PaxDbiQ9QYK4.
    PRIDEiQ9QYK4.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000065904; ENSMUSP00000070394; ENSMUSG00000053465.
    GeneIDi50787.
    KEGGimmu:50787.
    UCSCiuc007uzn.2. mouse.

    Organism-specific databases

    CTDi266722.
    MGIiMGI:1354960. Hs6st3.

    Phylogenomic databases

    eggNOGiKOG3955. Eukaryota.
    ENOG410XSW4. LUCA.
    GeneTreeiENSGT00390000013468.
    HOGENOMiHOG000007772.
    HOVERGENiHBG083012.
    InParanoidiQ9QYK4.
    KOiK08103.
    OMAiSSMNESQ.
    OrthoDBiEOG75MVW3.
    TreeFamiTF312835.

    Miscellaneous databases

    PROiQ9QYK4.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ9QYK4.
    CleanExiMM_HS6ST3.
    GenevisibleiQ9QYK4. MM.

    Family and domain databases

    InterProiIPR010635. Heparan_SO4-6-sulfoTrfase.
    IPR027417. P-loop_NTPase.
    IPR005331. Sulfotransferase.
    [Graphical view]
    PANTHERiPTHR12812. PTHR12812. 2 hits.
    PfamiPF03567. Sulfotransfer_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "The occurrence of three isoforms of heparan sulfate 6-O-sulfotransferase having different specificities for hexuronic acid adjacent to the targeted N-sulfoglucosamine."
      Habuchi H., Tanaka M., Habuchi O., Yoshida K., Suzuki H., Ban K., Kimata K.
      J. Biol. Chem. 275:2859-2868(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiH6ST3_MOUSE
    AccessioniPrimary (citable) accession number: Q9QYK4
    Secondary accession number(s): Q148Q1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2005
    Last sequence update: July 27, 2011
    Last modified: June 8, 2016
    This is version 84 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.