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Protein

Heparan-sulfate 6-O-sulfotransferase 3

Gene

Hs6st3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate.

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 6-sulfate.

Kineticsi

  1. KM=20 µM for CDSNS-heparin1 Publication
  2. KM=20 µM for NS-heparosan1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei198SubstrateBy similarity1
    Binding sitei203SubstrateBy similarity1
    Active sitei208Proton acceptorBy similarity1
    Binding sitei208SubstrateBy similarity1
    Binding sitei244PAPSBy similarity1
    Binding sitei252PAPSBy similarity1
    Binding sitei256SubstrateBy similarity1
    Binding sitei263SubstrateBy similarity1

    GO - Molecular functioni

    • heparan sulfate 6-O-sulfotransferase activity Source: MGI

    GO - Biological processi

    Keywordsi

    Molecular functionTransferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Heparan-sulfate 6-O-sulfotransferase 3 (EC:2.8.2.-)
    Short name:
    HS6ST-3
    Short name:
    mHS6ST-3
    Gene namesi
    Name:Hs6st3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 14

    Organism-specific databases

    MGIiMGI:1354960. Hs6st3.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 4CytoplasmicSequence analysis4
    Transmembranei5 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST23
    Topological domaini28 – 470LumenalSequence analysisAdd BLAST443

    GO - Cellular componenti

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001908101 – 470Heparan-sulfate 6-O-sulfotransferase 3Add BLAST470

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi127N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi230N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi323N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi328N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi379N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiQ9QYK4.
    PRIDEiQ9QYK4.

    PTM databases

    iPTMnetiQ9QYK4.
    PhosphoSitePlusiQ9QYK4.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed.1 Publication

    Gene expression databases

    BgeeiENSMUSG00000053465.
    CleanExiMM_HS6ST3.
    GenevisibleiQ9QYK4. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000070394.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QYK4.
    SMRiQ9QYK4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni151 – 159PAPS bindingBy similarity9
    Regioni181 – 182Substrate bindingBy similarity2
    Regioni376 – 378PAPS bindingBy similarity3
    Regioni382 – 383PAPS bindingBy similarity2

    Sequence similaritiesi

    Belongs to the sulfotransferase 6 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3955. Eukaryota.
    ENOG410XSW4. LUCA.
    GeneTreeiENSGT00390000013468.
    HOGENOMiHOG000007772.
    HOVERGENiHBG083012.
    InParanoidiQ9QYK4.
    KOiK08103.
    OMAiTVTEDYN.
    OrthoDBiEOG091G08UD.
    TreeFamiTF312835.

    Family and domain databases

    InterProiView protein in InterPro
    IPR010635. Heparan_SO4-6-sulfoTrfase.
    IPR027417. P-loop_NTPase.
    IPR005331. Sulfotransferase.
    PANTHERiPTHR12812. PTHR12812. 1 hit.
    PfamiView protein in Pfam
    PF03567. Sulfotransfer_2. 1 hit.
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9QYK4-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MDERFNKWLL TPVLTLLFVV IMYQYVSPSC TSSCTNFGEQ LRSGEARPPA
    60 70 80 90 100
    VPSPARRAQA PLDEWERRPQ LPPPPRGPPE GSRGVAAPED EDEDPGDPEE
    110 120 130 140 150
    EEEEEEEEPD PEAPENGSLP RFVPRFNFTL KDLTRFVDFN IKGRDVIVFL
    160 170 180 190 200
    HIQKTGGTTF GRHLVKNIRL EQPCSCKAGQ KKCTCHRPGK KETWLFSRFS
    210 220 230 240 250
    TGWSCGLHAD WTELTNCVPA IMEKKDCPRN HSHTRNFYYI TMLRDPVSRY
    260 270 280 290 300
    LSEWKHVQRG ATWKTSLHMC DGRSPTPDEL PTCYPGDDWS GVSLREFMDC
    310 320 330 340 350
    SYNLANNRQV RMLADLSLVG CYNLTFMNES ERNTILLQSA KNNLKNMAFF
    360 370 380 390 400
    GLTEFQRKTQ FLFERTFNLK FISPFTQFNI TRASNVDIND GARQHIEELN
    410 420 430 440 450
    FLDMQLYEYA KDLFQQRYHH TKQLEHQRDR QKRREERRLQ REHRAHRWPK
    460 470
    EDRAMEGTVT EDYNSQVVRW
    Length:470
    Mass (Da):55,037
    Last modified:July 27, 2011 - v2
    Checksum:i195B376684A4328D
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti16L → F in BAA89249 (PubMed:10644753).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB024567 mRNA. Translation: BAA89249.1.
    BC118035 mRNA. Translation: AAI18036.1.
    CCDSiCCDS27339.1.
    RefSeqiNP_056635.2. NM_015820.3.
    UniGeneiMm.445777.

    Genome annotation databases

    EnsembliENSMUST00000065904; ENSMUSP00000070394; ENSMUSG00000053465.
    GeneIDi50787.
    KEGGimmu:50787.
    UCSCiuc007uzn.2. mouse.

    Similar proteinsi

    Entry informationi

    Entry nameiH6ST3_MOUSE
    AccessioniPrimary (citable) accession number: Q9QYK4
    Secondary accession number(s): Q148Q1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2005
    Last sequence update: July 27, 2011
    Last modified: August 30, 2017
    This is version 93 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families