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Protein

ATP-binding cassette sub-family B member 9

Gene

Abcb9

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent low-affinity peptide transporter which translocates a broad spectrum of peptides from the cytosol to the lysosomal lumen. Displays a broad peptide length specificity from 6-mer up to at least 59-mer peptides with an optimum of 23-mers. Favors positively charged, aromatic or hydrophobic residues in the N- and C-terminal positions whereas negatively charged residues as well as asparagine and methionine are not favored (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi535 – 5428ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Peptide transport, Protein transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette sub-family B member 9
Alternative name(s):
ATP-binding cassette transporter 9
Short name:
ABC transporter 9 protein
TAP-like protein
Short name:
TAPL
Gene namesi
Name:Abcb9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620254. Abcb9.

Subcellular locationi

  • Lysosome membrane By similarity; Multi-pass membrane protein PROSITE-ProRule annotation

  • Note: May be located in membrane rafts.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei47 – 6721HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei84 – 10421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei116 – 13621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei181 – 20121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei221 – 24121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei315 – 33521HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei412 – 43221HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  • ATP-binding cassette (ABC) transporter complex Source: RGD
  • lysosomal membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 762762ATP-binding cassette sub-family B member 9PRO_0000000254Add
BLAST

Proteomic databases

PaxDbiQ9QYJ4.
PRIDEiQ9QYJ4.

PTM databases

PhosphoSiteiQ9QYJ4.

Expressioni

Tissue specificityi

Found in testis, particularly in the Sertoli cells of the seminiferous tubules. Also expressed in kidney, brain, heart, lung, spleen, thymus, intestine and testis. Higher expression detected in brain and testis than in thymus and intestine.2 Publications

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051058.

Structurei

3D structure databases

ProteinModelPortaliQ9QYJ4.
SMRiQ9QYJ4. Positions 489-735.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini184 – 467284ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST
Domaini500 – 736237ABC transporterPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi380 – 3856Poly-Glu

Domaini

The N-terminal region comprising the first four transmembrane domains is required for lysosomal localization but not for homodimerization or peptide transport.By similarity

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation
Contains 1 ABC transporter domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0058. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG008358.
InParanoidiQ9QYJ4.
KOiK05656.
OrthoDBiEOG7Z3F4H.
PhylomeDBiQ9QYJ4.
TreeFamiTF105197.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030254. ABCB9.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24221:SF242. PTHR24221:SF242. 1 hit.
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QYJ4-1) [UniParc]FASTAAdd to basket

Also known as: TAPLa, C-I

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLWKAVVVT LAFVSMDVGV TTAIYAFSHL DRSLLEDIRH FNIFDSVLDL
60 70 80 90 100
WAACLYRSCL LLGATIGVAK NSALGPRRLR ASWLVITLVC LFVGIYAMAK
110 120 130 140 150
LLLFSEVRRP IRDPWFWALF VWTYISLAAS FLLWGLLATV RPDAEALEPG
160 170 180 190 200
NEGFHGEGGA PAEQASGATL QKLLSYTKPD VAFLVAASFF LIVAALGETF
210 220 230 240 250
LPYYTGRAID SIVIQKSMDQ FTTAVVVVCL LAIGSSLAAG IRGGIFTLVF
260 270 280 290 300
ARLNIRLRNC LFRSLVSQET SFFDENRTGD LISRLTSDTT MVSDLVSQNI
310 320 330 340 350
NIFLRNTVKV TGVVVFMFSL SWQLSLVTFM GFPIIMMVSN IYGKYYKRLS
360 370 380 390 400
KEVQSALARA STTAEETISA MKTVRSFANE EEEAEVFLRK LQQVYKLNRK
410 420 430 440 450
EAAAYMSYVW GSGLTLLVVQ VSILYYGGHL VISGQMSSGN LIAFIIYEFV
460 470 480 490 500
LGDCMESVGS VYSGLMQGVG AAEKVFEFID RQPTMVHDGR LAPDHLEGRV
510 520 530 540 550
DFENVTFTYR TRPHTQVLQN VSFSLSPGKV TALVGPSGSG KSSCVNILEN
560 570 580 590 600
FYPLQGGRVL LDGEPIGAYD HKYLHRVISL VSQEPVLFAR SITDNISYGL
610 620 630 640 650
PTVPFEMVVE AAQKANAHGF IMELQDGYST ETGEKGAQLS GGQKQRVAMA
660 670 680 690 700
RALVRNPPVL ILDEATSALD AESEYLIQQA IHGNLQRHTV LIIAHRLSTV
710 720 730 740 750
ERAHLIVVLD KGRVVQQGTH QQLLAQGGLY AKLVQRQMLG LEHPLDYTAG
760
HKEPPSNTEH KA
Length:762
Mass (Da):84,033
Last modified:May 1, 2000 - v1
Checksum:iE39D3B42CFBD88EF
GO
Isoform 2 (identifier: Q9QYJ4-2) [UniParc]FASTAAdd to basket

Also known as: TAPLb, C-II

The sequence of this isoform differs from the canonical sequence as follows:
     721-762: QQLLAQGGLY...EPPSNTEHKA → LPLLSTPATL...WFKILGELGQ

Show »
Length:766
Mass (Da):84,281
Checksum:i9EFF44B28F15AF0E
GO
Isoform 3 (identifier: Q9QYJ4-3) [UniParc]FASTAAdd to basket

Also known as: C-III

The sequence of this isoform differs from the canonical sequence as follows:
     677-762: IQQAIHGNLQ...EPPSNTEHKA → VALADLKYREIHPSPQD

Show »
Length:693
Mass (Da):76,377
Checksum:iEEDEDCB2071256CB
GO
Isoform 4 (identifier: Q9QYJ4-4) [UniParc]FASTAAdd to basket

Also known as: C-IV

The sequence of this isoform differs from the canonical sequence as follows:
     677-762: IQQAIHGNLQ...EPPSNTEHKA → SEAQTPGLEHTPHPATTAWLEATLSPKPGCQG

Show »
Length:708
Mass (Da):77,739
Checksum:iE1B48C06C6215E14
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei677 – 76286IQQAI…TEHKA → VALADLKYREIHPSPQD in isoform 3. 1 PublicationVSP_041888Add
BLAST
Alternative sequencei677 – 76286IQQAI…TEHKA → SEAQTPGLEHTPHPATTAWL EATLSPKPGCQG in isoform 4. 1 PublicationVSP_041887Add
BLAST
Alternative sequencei721 – 76242QQLLA…TEHKA → LPLLSTPATLQRNLVCKMYS QIGGGDRGNAQGLALSWFKI LGELGQ in isoform 2. 1 PublicationVSP_000034Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027520 mRNA. Translation: BAA85306.1.
AB116264 mRNA. Translation: BAD10852.1.
AB116265 mRNA. Translation: BAD10853.1.
RefSeqiNP_071574.1. NM_022238.1. [Q9QYJ4-1]
UniGeneiRn.44870.

Genome annotation databases

GeneIDi63886.
KEGGirno:63886.
UCSCiRGD:620254. rat. [Q9QYJ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB027520 mRNA. Translation: BAA85306.1.
AB116264 mRNA. Translation: BAD10852.1.
AB116265 mRNA. Translation: BAD10853.1.
RefSeqiNP_071574.1. NM_022238.1. [Q9QYJ4-1]
UniGeneiRn.44870.

3D structure databases

ProteinModelPortaliQ9QYJ4.
SMRiQ9QYJ4. Positions 489-735.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000051058.

PTM databases

PhosphoSiteiQ9QYJ4.

Proteomic databases

PaxDbiQ9QYJ4.
PRIDEiQ9QYJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi63886.
KEGGirno:63886.
UCSCiRGD:620254. rat. [Q9QYJ4-1]

Organism-specific databases

CTDi23457.
RGDi620254. Abcb9.

Phylogenomic databases

eggNOGiKOG0058. Eukaryota.
COG1132. LUCA.
HOVERGENiHBG008358.
InParanoidiQ9QYJ4.
KOiK05656.
OrthoDBiEOG7Z3F4H.
PhylomeDBiQ9QYJ4.
TreeFamiTF105197.

Miscellaneous databases

PROiQ9QYJ4.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR030254. ABCB9.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24221:SF242. PTHR24221:SF242. 1 hit.
PfamiPF00664. ABC_membrane. 1 hit.
PF00005. ABC_tran. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF90123. SSF90123. 1 hit.
PROSITEiPS50929. ABC_TM1F. 1 hit.
PS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "An ABC transporter homologous to TAP proteins."
    Yamaguchi Y., Kasano M., Terada T., Sato R., Maeda M.
    FEBS Lett. 457:231-236(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), TISSUE SPECIFICITY.
    Tissue: Intestine and Kidney.
  2. "The carboxyl terminal sequence of rat transporter associated with antigen processing (TAP)-like (ABCB9) is heterogeneous due to splicing of its mRNA."
    Yamaguchi Y., Iseoka H., Kobayashi A., Maeda M.
    Biol. Pharm. Bull. 27:100-104(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 4), TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
  3. "Biochemical characterization of transporter associated with antigen processing (TAP)-like (ABCB9) expressed in insect cells."
    Ohara T., Ohashi-Kobayashi A., Maeda M.
    Biol. Pharm. Bull. 31:1-5(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.

Entry informationi

Entry nameiABCB9_RAT
AccessioniPrimary (citable) accession number: Q9QYJ4
Secondary accession number(s): Q764Q5, Q764Q6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 2, 2002
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.