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Protein

DnaJ homolog subfamily B member 2

Gene

Dnajb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family. In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins. Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes. Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 2Curated
Alternative name(s):
DnaJ homolog subfamily B member 101 Publication
mDj8Imported
Gene namesi
Name:Dnajb2Imported
Synonyms:Dnajb101 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1928739. Dnajb2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00004386882 – 321DnaJ homolog subfamily B member 2Add BLAST320
PropeptideiPRO_0000438689322 – 324Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei311PhosphoserineCombined sources1
Modified residuei321Cysteine methyl esterBy similarity1
Lipidationi321S-geranylgeranyl cysteineBy similarity1

Post-translational modificationi

Ubiquitinated by STUB1; does not lead to proteasomal degradation.By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation, Ubl conjugation

Proteomic databases

MaxQBiQ9QYI5.
PaxDbiQ9QYI5.
PeptideAtlasiQ9QYI5.
PRIDEiQ9QYI5.

PTM databases

iPTMnetiQ9QYI5.
PhosphoSitePlusiQ9QYI5.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026203.
ExpressionAtlasiQ9QYI5. baseline and differential.
GenevisibleiQ9QYI5. MM.

Interactioni

Subunit structurei

Interacts with HSP70 (HSPA1A or HSPA1B). Interacts with HSPA8/Hsc70. Interacts with PSMA3 and most probably with the whole proteasomal complex.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000080796.

Structurei

3D structure databases

ProteinModelPortaliQ9QYI5.
SMRiQ9QYI5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 69JPROSITE-ProRule annotationAdd BLAST67
Domaini207 – 226UIM 1PROSITE-ProRule annotationAdd BLAST20
Domaini250 – 269UIM 2PROSITE-ProRule annotationAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi321 – 324CAAX motifBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi141 – 170Ser-richPROSITE-ProRule annotationAdd BLAST30

Domaini

The J domain is sufficient to interact with HSP70 (HSPA1A or HSPA1B) and activate its ATPase activity. The J domain is also required for the HSP70-mediated and ubiquitin-dependent proteasomal degradation of proteins like ATXN3. The J domain is required to reduce PRKN cytoplasmic aggregation.By similarity
The UIM domains mediate interaction with ubiquitinated chaperone clients and with the proteasome. The UIM domains may have an opposite activity to the J domain, binding ubiquitinated proteins and protecting them from HSP70-mediated proteasomal degradation. The UIM domains are not required to reduce PRKN cytoplasmic aggregation.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0714. Eukaryota.
COG0484. LUCA.
GeneTreeiENSGT00760000118947.
HOGENOMiHOG000111538.
HOVERGENiHBG066998.
InParanoidiQ9QYI5.
KOiK09508.
OMAiTFTFRNP.
OrthoDBiEOG091G152F.
TreeFamiTF105142.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiView protein in InterPro
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR003903. UIM_dom.
PfamiView protein in Pfam
PF00226. DnaJ. 1 hit.
PF02809. UIM. 2 hits.
PRINTSiPR00625. JDOMAIN.
SMARTiView protein in SMART
SM00271. DnaJ. 1 hit.
SM00726. UIM. 2 hits.
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiView protein in PROSITE
PS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
PS50330. UIM. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QYI5-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASYYEILDV PRSASPDDIK KAYRKKALQW HPDKNPDNKE FAEKKFKEVA
60 70 80 90 100
EAYEVLSDKH KREIYDRYGR EGLTGAGSGP SRSETGGAGP GFTFTFRSPE
110 120 130 140 150
EVFREFFGSG DPFSELFDDL GVFSELQNQG PRLTGPFFTF SSSFPANSDF
160 170 180 190 200
SSSSFSFSPG AGAFRSVSTS TTFVQGRRIT TRRIMENGQE RVEVEEDGQL
210 220 230 240 250
KSVSINGVPD DLALGLELSR REQQPSVAPG LGVMQVRPTS LSRPPDHDLS
260 270 280 290 300
EDEDLQLAMA YSLSEMEAAG QKPAGGRGAQ QRQHGQPKAQ HRDLDVGGTH
310 320
KSVRGEAAKL SPSSEEKASR CHIL
Length:324
Mass (Da):35,593
Last modified:January 18, 2017 - v3
Checksum:iC00006AAC12574FA
GO
Isoform 2 (identifier: Q9QYI5-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     275-277: GGR → DVF
     278-324: Missing.

Show »
Length:277
Mass (Da):30,615
Checksum:i989193A574FA1AD2
GO
Isoform 3 (identifier: Q9QYI5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     59-77: KHKREIYDRYGREGLTGAG → R
     275-277: GGR → DVF
     278-324: Missing.

Show »
Length:259
Mass (Da):28,583
Checksum:i0DD1670B36FD6108
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti15S → F in AAH11090 (PubMed:15489334).Curated1
Sequence conflicti102V → F in BAA88306 (PubMed:11147971).Curated1
Sequence conflicti111D → N in BAA88306 (PubMed:11147971).Curated1
Sequence conflicti183R → K in BAA88306 (PubMed:11147971).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05870859 – 77KHKRE…LTGAG → R in isoform 3. Add BLAST19
Alternative sequenceiVSP_058709275 – 277GGR → DVF in isoform 3 and isoform 2. 3
Alternative sequenceiVSP_058710278 – 324Missing in isoform 3 and isoform 2. Add BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028858 mRNA. Translation: BAA88306.1.
AK076061 mRNA. Translation: BAC36155.1.
AK156083 mRNA. Translation: BAE33579.1.
AK171494 mRNA. Translation: BAE42490.1.
AC166150 Genomic DNA. No translation available.
BC011090 mRNA. Translation: AAH11090.1.
CCDSiCCDS15068.1. [Q9QYI5-2]
CCDS35624.1. [Q9QYI5-1]
CCDS78622.1. [Q9QYI5-3]
RefSeqiNP_001153355.1. NM_001159883.1. [Q9QYI5-3]
NP_001153356.1. NM_001159884.1. [Q9QYI5-1]
NP_001153357.1. NM_001159885.1. [Q9QYI5-1]
NP_064662.2. NM_020266.2. [Q9QYI5-2]
NP_835156.1. NM_178055.4. [Q9QYI5-1]
UniGeneiMm.489660.

Genome annotation databases

EnsembliENSMUST00000055223; ENSMUSP00000052520; ENSMUSG00000026203. [Q9QYI5-2]
ENSMUST00000082158; ENSMUSP00000080796; ENSMUSG00000026203. [Q9QYI5-1]
ENSMUST00000187058; ENSMUSP00000140637; ENSMUSG00000026203. [Q9QYI5-2]
ENSMUST00000188290; ENSMUSP00000140634; ENSMUSG00000026203. [Q9QYI5-1]
ENSMUST00000188346; ENSMUSP00000140588; ENSMUSG00000026203. [Q9QYI5-1]
ENSMUST00000188931; ENSMUSP00000140566; ENSMUSG00000026203. [Q9QYI5-3]
GeneIDi56812.
KEGGimmu:56812.
UCSCiuc007bom.2. mouse. [Q9QYI5-3]
uc007bon.2. mouse.
uc007bop.2. mouse. [Q9QYI5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDNJB2_MOUSE
AccessioniPrimary (citable) accession number: Q9QYI5
Secondary accession number(s): Q3TB24, Q8BPF6, Q921S2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: January 18, 2017
Last modified: September 27, 2017
This is version 127 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot