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Protein

DnaJ homolog subfamily B member 12

Gene

Dnajb12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a co-chaperone with HSPA8/Hsc70; required to promote protein folding and trafficking, prevent aggregation of client proteins, and promote unfolded proteins to endoplasmic reticulum-associated degradation (ERAD) pathway. Acts by determining HSPA8/Hsc70's ATPase and polypeptide-binding activities. Can also act independently of HSPA8/Hsc70: together with DNAJB14, acts as a chaperone that promotes maturation of potassium channels KCND2 and KCNH2 by stabilizing nascent channel subunits and assembling them into tetramers. While stabilization of nascent channel proteins is dependent on HSPA8/Hsc70, the process of oligomerization of channel subunits is independent of HSPA8/Hsc70. When overexpressed, forms membranous structures together with DNAJB14 and HSPA8/Hsc70 within the nucleus; the role of these structures, named DJANGOs, is still unclear.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
DnaJ homolog subfamily B member 12Imported
Alternative name(s):
mDj101 Publication
Gene namesi
Name:Dnajb12Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1931881 Dnajb12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000710381 – 376DnaJ homolog subfamily B member 12Add BLAST376

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9QYI4
MaxQBiQ9QYI4
PaxDbiQ9QYI4
PeptideAtlasiQ9QYI4
PRIDEiQ9QYI4

PTM databases

iPTMnetiQ9QYI4
PhosphoSitePlusiQ9QYI4

Expressioni

Gene expression databases

BgeeiENSMUSG00000020109
CleanExiMM_DNAJB12
ExpressionAtlasiQ9QYI4 baseline and differential
GenevisibleiQ9QYI4 MM

Interactioni

Subunit structurei

Homodimer and homotetramer. Interacts (via J domain) with HSPA8/Hsc70. Forms a multiprotein complex, at least composed of DNAJB12, DNAJB14, HSPA8/Hsc70 and SGTA; interaction with DNAJB14 and HSPA8/Hsc70 is direct.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi208137, 1 interactor
IntActiQ9QYI4, 1 interactor
STRINGi10090.ENSMUSP00000020309

Structurei

3D structure databases

ProteinModelPortaliQ9QYI4
SMRiQ9QYI4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini111 – 175JPROSITE-ProRule annotationAdd BLAST65

Sequence similaritiesi

Belongs to the DnaJ family. DNAJB12/DNAJB14 subfamily.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0714 Eukaryota
COG0484 LUCA
GeneTreeiENSGT00840000129767
HOGENOMiHOG000233608
HOVERGENiHBG051373
InParanoidiQ9QYI4
KOiK09518
PhylomeDBiQ9QYI4
TreeFamiTF105145

Family and domain databases

CDDicd06257 DnaJ, 1 hit
Gene3Di1.10.287.110, 1 hit
InterProiView protein in InterPro
IPR001623 DnaJ_domain
IPR018253 DnaJ_domain_CS
IPR015399 DUF1977_DnaJ-like
IPR036869 J_dom_sf
PfamiView protein in Pfam
PF00226 DnaJ, 1 hit
PF09320 DUF1977, 1 hit
PRINTSiPR00625 JDOMAIN
SMARTiView protein in SMART
SM00271 DnaJ, 1 hit
SUPFAMiSSF46565 SSF46565, 1 hit
PROSITEiView protein in PROSITE
PS00636 DNAJ_1, 1 hit
PS50076 DNAJ_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QYI4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESNKDEAER CISIALKAIQ SNQPERALRF LEKAQRLYPT PRVSALIESL
60 70 80 90 100
NQKPQSTGDH PQPTDTTHTT TKKAGGTETP SANGEAGGGE SAKGYTSEQV
110 120 130 140 150
AAVKRVKQCK DYYEILGVSR SASDEDLKKA YRKLALKFHP DKNHAPGATE
160 170 180 190 200
AFKAIGTAYA VLSNPEKRKQ YDQFGDDKSQ AARHGHSHGD FHRGFEADIS
210 220 230 240 250
PEDLFNMFFG GGFPSSNVHV YSNGRMRYTY QQRQDRRDNQ GDGGLGVFVQ
260 270 280 290 300
LMPILILILV SALSQLMVSS PPYSLSPRPS VGHIHKRVTD HLNVAYYVAD
310 320 330 340 350
TFSEEYTGSS LKTVERNVED DYIANLRNNC WKEKQQKEGL LYRARYFGDT
360 370
DMYHRAQKMG TPSCNRLSEV QASLHG
Length:376
Mass (Da):41,988
Last modified:July 27, 2011 - v2
Checksum:i33560A17E4B14235
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti321D → E in BAA88308 (PubMed:11147971).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028860 mRNA Translation: BAA88308.1
AK138495 mRNA Translation: BAE23683.1
AK166224 mRNA Translation: BAE38642.1
AK172274 mRNA Translation: BAE42919.1
CH466553 Genomic DNA Translation: EDL32192.1
CH466553 Genomic DNA Translation: EDL32193.1
CH466553 Genomic DNA Translation: EDL32195.1
BC034162 mRNA Translation: AAH34162.1
CCDSiCCDS23867.1
RefSeqiNP_064349.2, NM_019965.2
UniGeneiMm.103610
Mm.443586

Genome annotation databases

EnsembliENSMUST00000020309; ENSMUSP00000020309; ENSMUSG00000020109
ENSMUST00000142819; ENSMUSP00000118088; ENSMUSG00000020109
ENSMUST00000146590; ENSMUSP00000122056; ENSMUSG00000020109
GeneIDi56709
KEGGimmu:56709
UCSCiuc007fdz.1 mouse

Similar proteinsi

Entry informationi

Entry nameiDJB12_MOUSE
AccessioniPrimary (citable) accession number: Q9QYI4
Secondary accession number(s): Q8K037
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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