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Protein

Melanoma-associated antigen D1

Gene

Maged1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Plays a role in the circadian rhythm regulation. May act as RORA coregulator, modulating the expression of core clock genes such as ARNTL/BMAL1 and NFIL3, induced, or NR1D1, repressed.1 Publication

GO - Molecular functioni

  • transcription coactivator activity Source: MGI

GO - Biological processi

  • circadian regulation of gene expression Source: UniProtKB
  • extrinsic apoptotic signaling pathway in absence of ligand Source: Reactome
  • negative regulation of epithelial cell proliferation Source: MGI
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of apoptotic process Source: Reactome
  • positive regulation of apoptotic signaling pathway Source: MGI
  • positive regulation of branching involved in ureteric bud morphogenesis Source: UniProtKB
  • positive regulation of MAP kinase activity Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • regulation of circadian rhythm Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Tumor antigen

Keywords - Biological processi

Biological rhythms, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-418889. Ligand-independent caspase activation via DCC.

Names & Taxonomyi

Protein namesi
Recommended name:
Melanoma-associated antigen D1
Alternative name(s):
Dlxin-1
MAGE-D1 antigen
Neurotrophin receptor-interacting MAGE homolog
Gene namesi
Name:Maged1
Synonyms:Nrage
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1930187. Maged1.

Subcellular locationi

GO - Cellular componenti

  • chromatin Source: UniProtKB
  • cytosol Source: Reactome
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB-SubCell
  • protein complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Animals show shortened circadian period and reduced total activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001567241 – 775Melanoma-associated antigen D1Add BLAST775

Proteomic databases

MaxQBiQ9QYH6.
PaxDbiQ9QYH6.
PRIDEiQ9QYH6.

PTM databases

iPTMnetiQ9QYH6.
PhosphoSitePlusiQ9QYH6.

Expressioni

Tissue specificityi

Ubiquitously expressed in many adult tissues, except for the spleen. Expressed in osteoblastic and chondrogenic cell lines and also during embryonic development.

Gene expression databases

BgeeiENSMUSG00000025151.
CleanExiMM_MAGED1.
GenevisibleiQ9QYH6. MM.

Interactioni

Subunit structurei

Interacts with DLX5, DLX7 and MSX2 and forms homomultimers. Interacts with UNC5A. Interacts with TRIM28 and PJA1. Interacts with NGFR/p75NTR and RORA.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Dlx5P703962EBI-1801274,EBI-1801294
Msx2Q033585EBI-1801274,EBI-1801354
NdnP252337EBI-1801274,EBI-1801080
Pja1O55176-22EBI-1801274,EBI-1801670
RoraP514485EBI-1801274,EBI-1169722

Protein-protein interaction databases

BioGridi220497. 7 interactors.
IntActiQ9QYH6. 10 interactors.
STRINGi10090.ENSMUSP00000026142.

Structurei

3D structure databases

ProteinModelPortaliQ9QYH6.
SMRiQ9QYH6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati292 – 29716
Repeati298 – 30326
Repeati304 – 30936
Repeati329 – 33446
Repeati335 – 34056
Repeati341 – 34666
Repeati347 – 35276
Repeati353 – 35886
Repeati359 – 36496
Repeati365 – 370106
Repeati371 – 376116
Repeati377 – 382126
Repeati383 – 388136
Repeati389 – 394146
Repeati395 – 400156
Repeati401 – 406166
Repeati407 – 412176
Repeati413 – 418186
Repeati419 – 424196
Repeati425 – 42920; approximate5
Repeati430 – 435216
Repeati436 – 441226
Domaini468 – 666MAGEPROSITE-ProRule annotationAdd BLAST199

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni292 – 44122 X 6 AA tandem repeats of W-[PQ]-X-P-X-XAdd BLAST150

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi276 – 462Pro-richAdd BLAST187

Sequence similaritiesi

Contains 1 MAGE domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000049038.
HOVERGENiHBG003714.
InParanoidiQ9QYH6.
KOiK12464.
OMAiWQNPVIW.
OrthoDBiEOG091G09D3.
PhylomeDBiQ9QYH6.
TreeFamiTF352132.

Family and domain databases

InterProiIPR030083. MAGED1.
IPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736:SF28. PTHR11736:SF28. 1 hit.
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QYH6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQKPDGGAG LRGFQAEASV EDSALLVQTL MEAIQISEAP PTSQATAAAS
60 70 80 90 100
GPNASPQSSQ PPTANEKADT EVSAAAARPK TGFKAQNATT KGPNDYSQAR
110 120 130 140 150
NAKEMPKNQS KAAFKSQNGT PKGPHAASDF SQAAPTGKSA KKSEMAFKGQ
160 170 180 190 200
NSTKAGPGTT YNFPQSPSAN EMTNNQPKTA KAWNDTTKVP GADAQTQNVN
210 220 230 240 250
QAKMADVGTS AGISEADGAA AQTSADGSQT QNVESRTIIR GKRTRKVNNL
260 270 280 290 300
NVEENNSGDQ RRASLASGNW RSAPVPVTTQ QNPPGAPPNV VWQTPLAWQN
310 320 330 340 350
PSGWQNQTAR QTPPAARQSP PARQTPSAWQ NPVAWQNPVI WPNPVIWQNP
360 370 380 390 400
VIWPNPIVWP GPIVWPNPMA WQSTPGWQSP PSWQAPPSWQ SPQDWQGPPD
410 420 430 440 450
WQVPPDWSMP PDWSFPSDWP FPPDWIPADW PIPPDWQNLR PSPNLRSSSN
460 470 480 490 500
SRASQNQGPP QPRDVALLQE RANKLVKYLM LKDYTKVPIK RSEMLRDIIR
510 520 530 540 550
EYTDVYPEII ERACFVLEKK FGIQLKEIDK EEHLYILIST PESLAGILGT
560 570 580 590 600
TKDTPKLGLL LVILGIIFMN GNRATEAVLW EALRKMGLRP GVRHPLLGDL
610 620 630 640 650
RKLLTYEFVK QKYLDYRRVP NSNPPEYEFL WGLRSYHETS KMKVLRFIAE
660 670 680 690 700
VQKRDPRDWT AQFMEAADEA LDALDAAAAE AEARAEARNR MGIGDEAVSG
710 720 730 740 750
PWSWDDIEFE LLTWDEEGDF GDPWSRIPFT FWARYHQNAR SRFPQAFTGP
760 770
IIGPSGTATA NFAANFGAIG FFWVE
Length:775
Mass (Da):85,670
Last modified:May 1, 2000 - v1
Checksum:i224B82470816835A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti357 – 362Missing in AAK01203 (Ref. 2) Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029448 mRNA. Translation: BAA87959.1.
AF319975 mRNA. Translation: AAK01203.1.
AK013231 mRNA. No translation available.
AK017275 mRNA. Translation: BAB30666.1.
AK049660 mRNA. Translation: BAC33863.1.
AK132248 mRNA. Translation: BAE21057.1.
AK145872 mRNA. Translation: BAE26714.1.
AK146908 mRNA. Translation: BAE27521.1.
CCDSiCCDS30280.1.
RefSeqiNP_062765.1. NM_019791.2.
UniGeneiMm.27578.

Genome annotation databases

EnsembliENSMUST00000026142; ENSMUSP00000026142; ENSMUSG00000025151.
GeneIDi94275.
KEGGimmu:94275.
UCSCiuc009ttn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB029448 mRNA. Translation: BAA87959.1.
AF319975 mRNA. Translation: AAK01203.1.
AK013231 mRNA. No translation available.
AK017275 mRNA. Translation: BAB30666.1.
AK049660 mRNA. Translation: BAC33863.1.
AK132248 mRNA. Translation: BAE21057.1.
AK145872 mRNA. Translation: BAE26714.1.
AK146908 mRNA. Translation: BAE27521.1.
CCDSiCCDS30280.1.
RefSeqiNP_062765.1. NM_019791.2.
UniGeneiMm.27578.

3D structure databases

ProteinModelPortaliQ9QYH6.
SMRiQ9QYH6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220497. 7 interactors.
IntActiQ9QYH6. 10 interactors.
STRINGi10090.ENSMUSP00000026142.

PTM databases

iPTMnetiQ9QYH6.
PhosphoSitePlusiQ9QYH6.

Proteomic databases

MaxQBiQ9QYH6.
PaxDbiQ9QYH6.
PRIDEiQ9QYH6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026142; ENSMUSP00000026142; ENSMUSG00000025151.
GeneIDi94275.
KEGGimmu:94275.
UCSCiuc009ttn.1. mouse.

Organism-specific databases

CTDi9500.
MGIiMGI:1930187. Maged1.

Phylogenomic databases

eggNOGiKOG4562. Eukaryota.
ENOG4111S70. LUCA.
GeneTreeiENSGT00760000118824.
HOGENOMiHOG000049038.
HOVERGENiHBG003714.
InParanoidiQ9QYH6.
KOiK12464.
OMAiWQNPVIW.
OrthoDBiEOG091G09D3.
PhylomeDBiQ9QYH6.
TreeFamiTF352132.

Enzyme and pathway databases

ReactomeiR-MMU-193648. NRAGE signals death through JNK.
R-MMU-418889. Ligand-independent caspase activation via DCC.

Miscellaneous databases

ChiTaRSiMaged1. mouse.
PROiQ9QYH6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025151.
CleanExiMM_MAGED1.
GenevisibleiQ9QYH6. MM.

Family and domain databases

InterProiIPR030083. MAGED1.
IPR002190. MHD_dom.
[Graphical view]
PANTHERiPTHR11736:SF28. PTHR11736:SF28. 1 hit.
PfamiPF01454. MAGE. 1 hit.
[Graphical view]
SMARTiSM01373. MAGE. 1 hit.
[Graphical view]
PROSITEiPS50838. MAGE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAGD1_MOUSE
AccessioniPrimary (citable) accession number: Q9QYH6
Secondary accession number(s): Q543L6, Q99PB5, Q9CYX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.