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Protein

Uridine-cytidine kinase 2

Gene

Uck2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine (By similarity).By similarity

Catalytic activityi

ATP + uridine = ADP + UMP.By similarity
ATP + cytidine = ADP + CMP.By similarity

Pathwayi: CTP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes CTP from cytidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Uridine-cytidine kinase 2 (Uck2), Uridine kinase (Uck2), Uridine-cytidine kinase (Uckl1), Uridine kinase (Uck1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway CTP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from cytidine, the pathway CTP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uridine.
Proteins known to be involved in this subpathway in this organism are:
  1. Uridine-cytidine kinase 2 (Uck2), Uridine kinase (Uck2), Uridine-cytidine kinase (Uckl1), Uridine kinase (Uck1)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uridine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei84 – 841SubstrateBy similarity
Binding sitei112 – 1121SubstrateBy similarity
Binding sitei117 – 1171SubstrateBy similarity
Binding sitei166 – 1661SubstrateBy similarity
Binding sitei176 – 1761SubstrateBy similarity
Binding sitei184 – 1841SubstrateBy similarity
Binding sitei213 – 2131ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 359ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • cellular response to oxygen levels Source: RGD
  • CMP salvage Source: InterPro
  • CTP salvage Source: UniProtKB-UniPathway
  • feeding behavior Source: RGD
  • protein deubiquitination Source: RGD
  • response to axon injury Source: RGD
  • UMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridine-cytidine kinase 2 (EC:2.7.1.48)
Short name:
UCK 2
Alternative name(s):
Cytidine monophosphokinase 2
Uridine monophosphokinase 2
Gene namesi
Name:Uck2Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620742. Uck2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: RGD
  • intracellular membrane-bounded organelle Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 261260Uridine-cytidine kinase 2PRO_0000164457Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei254 – 2541PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9QYG8.

Expressioni

Inductioni

Following crush injury to sciatic nerves.1 Publication

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005213.

Structurei

3D structure databases

ProteinModelPortaliQ9QYG8.
SMRiQ9QYG8. Positions 19-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the uridine kinase family.Sequence analysis

Phylogenomic databases

eggNOGiENOG410IMEH. Eukaryota.
COG0572. LUCA.
HOGENOMiHOG000262756.
HOVERGENiHBG023339.
InParanoidiQ9QYG8.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR029925. UCK-2.
IPR000764. Uridine_kinase-like.
[Graphical view]
PANTHERiPTHR10285:SF28. PTHR10285:SF28. 1 hit.
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QYG8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGDSEQTLQ NHQQPNGGEP FLIGVSGGTA SGKSSVCAKI VQLLGQNEVD
60 70 80 90 100
YHQKQVVILS QDSFYRVLTS EQKAKALKGQ FNFDHPDAFD NELIFKTLKE
110 120 130 140 150
ITEGKTVQIP VYDFVSHSRK EETVTIYPAD VVLFEGILAF YSQEVRDLFQ
160 170 180 190 200
MKLFVDTDAD TRLSRRVLRD ISERGRDLEQ ILSQYITFVK PAFEEFCLPT
210 220 230 240 250
KKYADVIIPR GADNLVAINL IVQHIQDILN GGLSKRQTNG YLNGYTPSRK
260
RQASESSSRP H
Length:261
Mass (Da):29,404
Last modified:September 2, 2008 - v2
Checksum:i780AA3CFF5CA8153
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473958 Genomic DNA. Translation: EDM09286.1.
BC167060 mRNA. Translation: AAI67060.1.
AB030700 mRNA. Translation: BAA83085.1.
UniGeneiRn.24811.

Genome annotation databases

UCSCiRGD:620742. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH473958 Genomic DNA. Translation: EDM09286.1.
BC167060 mRNA. Translation: AAI67060.1.
AB030700 mRNA. Translation: BAA83085.1.
UniGeneiRn.24811.

3D structure databases

ProteinModelPortaliQ9QYG8.
SMRiQ9QYG8. Positions 19-231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005213.

Proteomic databases

PaxDbiQ9QYG8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620742. rat.

Organism-specific databases

RGDi620742. Uck2.

Phylogenomic databases

eggNOGiENOG410IMEH. Eukaryota.
COG0572. LUCA.
HOGENOMiHOG000262756.
HOVERGENiHBG023339.
InParanoidiQ9QYG8.

Enzyme and pathway databases

UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.

Miscellaneous databases

PROiQ9QYG8.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR029925. UCK-2.
IPR000764. Uridine_kinase-like.
[Graphical view]
PANTHERiPTHR10285:SF28. PTHR10285:SF28. 1 hit.
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Up-regulated uridine kinase gene identified by RLCS in the ventral horn after crush injury to rat sciatic nerves."
    Yuh I., Yaoi T., Watanabe S., Okajima S., Hirasawa Y., Fushiki S.
    Biochem. Biophys. Res. Commun. 266:104-109(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 152-256, INDUCTION.
    Strain: Sprague-DawleyCurated.
    Tissue: Spinal cordImported.

Entry informationi

Entry nameiUCK2_RAT
AccessioniPrimary (citable) accession number: Q9QYG8
Secondary accession number(s): B2RZ83
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: September 2, 2008
Last modified: June 8, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.