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Q9QYG0 (NDRG2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein NDRG2
Alternative name(s):
N-myc downstream-regulated gene 2 protein
Protein Ndr2
Gene names
Name:Ndrg2
Synonyms:Kiaa1248, Ndr2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length371 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Contributes to the regulation of the Wnt signaling pathway. Down-regulates CTNNB1-mediated transcriptional activation of target genes, such as CCND1, and may thereby act as tumor suppressor. May be involved in dendritic cell and neuron differentiation By similarity.

Subunit structure

Interacts with CTNNB1 By similarity.

Subcellular location

Cytoplasm. Cytoplasmperinuclear region By similarity. Cell projectiongrowth cone By similarity. Note: In neurons, seems to concentrate at axonal growth cone. Perinuclear in neurons By similarity. Ref.7 Ref.9

Tissue specificity

Expressed at highest levels in brain, heart and liver, and at lower levels in kidney, colon, skeletal muscle, adrenal gland, ovary and uterus (at protein level). Ref.6 Ref.9 Ref.12

Developmental stage

Expression is restricted to the developing heart at the stage of 9.5 dpc, and increases after 11.5 dpc as development of tissues and organs proceeds. Present in many developing tissues including heart, brain, lung, liver, gut, kidney, skeletal muscle, cartilage and epidermis (at protein level). Ref.8 Ref.9

Sequence similarities

Belongs to the NDRG family.

Ontologies

Keywords
   Biological processDifferentiation
Neurogenesis
Wnt signaling pathway
   Cellular componentCell projection
Cytoplasm
   Coding sequence diversityAlternative splicing
   DiseaseTumor suppressor
   Molecular functionDevelopmental protein
   PTMAcetylation
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

cell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of ERK1 and ERK2 cascade

Inferred from mutant phenotype PubMed 19815093. Source: MGI

negative regulation of cytokine production

Inferred from mutant phenotype PubMed 19815093. Source: MGI

negative regulation of smooth muscle cell proliferation

Inferred from direct assay Ref.9. Source: MGI

nervous system development

Inferred from electronic annotation. Source: UniProtKB-KW

regulation of platelet-derived growth factor production

Inferred from mutant phenotype PubMed 19815093. Source: MGI

regulation of vascular endothelial growth factor production

Inferred from mutant phenotype PubMed 19815093. Source: MGI

   Cellular_componentGolgi apparatus

Inferred from electronic annotation. Source: Ensembl

centrosome

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from direct assay Ref.9. Source: MGI

growth cone

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay Ref.9. Source: MGI

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9QYG0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9QYG0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     26-39: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 371370Protein NDRG2
PRO_0000159576

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue3261Phosphoserine Ref.11
Modified residue3281Phosphoserine Ref.10 Ref.11
Modified residue3301Phosphothreonine; by SGK1 Ref.6 Ref.10 Ref.11
Modified residue3321Phosphoserine; by PKC/PRKCQ or SGK1 Ref.6 Ref.7 Ref.10 Ref.11
Modified residue3341Phosphothreonine Ref.10
Modified residue3351Phosphoserine Ref.10
Modified residue3381Phosphoserine Ref.10
Modified residue3481Phosphothreonine; by PKB/AKT1 or SGK1 Ref.6 Ref.7
Modified residue3501Phosphoserine Ref.6
Modified residue3571Phosphothreonine Ref.10

Natural variations

Alternative sequence26 – 3914Missing in isoform 2.
VSP_019009

Experimental info

Sequence conflict2531L → P in BAE39998. Ref.2
Sequence conflict3471R → S in BAE39998. Ref.2

Secondary structure

................................................. 371
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: A5237C04278197B2

FASTA37140,789
        10         20         30         40         50         60 
MAELQEVQIT EEKPLLPGQT PETAKEAELA ARILLDQGQT HSVETPYGSV TFTVYGTPKP 

        70         80         90        100        110        120 
KRPAIFTYHD VGLNYKSCFQ PLFRFGDMQE IIQNFVRVHV DAPGMEEGAP VFPLGYQYPS 

       130        140        150        160        170        180 
LDQLADMIPC ILQYLNFSTI IGVGVGAGAY ILSRYALNHP DTVEGLVLIN IDPNAKGWMD 

       190        200        210        220        230        240 
WAAHKLTGLT SSIPDMILGH LFSQEELSGN SELIQKYRGI IQHAPNLENI ELYWNSYNNR 

       250        260        270        280        290        300 
RDLNFERGGE TTLKCPVMLV VGDQAPHEDA VVECNSKLDP TQTSFLKMAD SGGQPQLTQP 

       310        320        330        340        350        360 
GKLTEAFKYF LQGMGYMASS CMTRLSRSRT ASLTSAASID GSRSRSRTLS QSSESGTLPS 

       370 
GPPGHTMEVS C 

« Hide

Isoform 2 [UniParc].

Checksum: E6A9E286AD92E7AC
Show »

FASTA35739,281

References

« Hide 'large scale' references
[1]"Identification of new genes ndr2 and ndr3 which are related to Ndr1/RTP/Drg1 but show distinct tissue specificity and response to N-myc."
Okuda T., Kondoh H.
Biochem. Biophys. Res. Commun. 266:208-215(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Embryo.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J, DBA/2 and NOD.
Tissue: Head and Visual cortex.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Liver.
[4]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 62-84; 155-176 AND 278-287, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[5]"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 64-371.
Tissue: Thymus.
[6]"Exploitation of KESTREL to identify NDRG family members as physiological substrates for SGK1 and GSK3."
Murray J.T., Campbell D.G., Morrice N., Auld G.C., Shpiro N., Marquez R., Peggie M., Bain J., Bloomberg G.B., Grahammer F., Lang F., Wulff P., Kuhl D., Cohen P.
Biochem. J. 384:477-488(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT THR-330; SER-332; THR-348 AND SER-350, TISSUE SPECIFICITY.
[7]"Akt mediates insulin-stimulated phosphorylation of Ndrg2: evidence for cross-talk with protein kinase C theta."
Burchfield J.G., Lennard A.J., Narasimhan S., Hughes W.E., Wasinger V.C., Corthals G.L., Okuda T., Kondoh H., Biden T.J., Schmitz-Peiffer C.
J. Biol. Chem. 279:18623-18632(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-332 AND THR-348, SUBCELLULAR LOCATION.
[8]"NDRG2: a novel Alzheimer's disease associated protein."
Mitchelmore C., Buechmann-Moller S., Rask L., West M.J., Troncoso J.C., Jensen N.A.
Neurobiol. Dis. 16:48-58(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[9]"Expression analysis of the NDRG2 gene in mouse embryonic and adult tissues."
Hu X.-L., Liu X.-P., Deng Y.-C., Lin S.-X., Wu L., Zhang J., Wang L.-F., Wang X.-B., Li X., Shen L., Zhang Y.-Q., Yao L.-B.
Cell Tissue Res. 325:67-76(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
[10]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-328; THR-330; SER-332; THR-334; SER-335; SER-338 AND THR-357, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[11]"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326; SER-328; THR-330 AND SER-332, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[12]"NDRG4 protein-deficient mice exhibit spatial learning deficits and vulnerabilities to cerebral ischemia."
Yamamoto H., Kokame K., Okuda T., Nakajo Y., Yanamoto H., Miyata T.
J. Biol. Chem. 286:26158-26165(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[13]"Crystal structure of the human N-Myc downstream-regulated gene 2 protein provides insight into its role as a tumor suppressor."
Hwang J., Kim Y., Kang H.B., Jaroszewski L., Deacon A.M., Lee H., Choi W.C., Kim K.J., Kim C.H., Kang B.S., Lee J.O., Oh T.K., Kim J.W., Wilson I.A., Kim M.H.
J. Biol. Chem. 286:12450-12460(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 40-313.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB033921 mRNA. Translation: BAA85882.1.
AK076514 mRNA. Translation: BAC36373.1.
AK154597 mRNA. Translation: BAE32700.1.
AK158900 mRNA. Translation: BAE34721.1.
AK168011 mRNA. Translation: BAE39998.1.
BC012963 mRNA. Translation: AAH12963.1.
AK173136 mRNA. Translation: BAD32414.1.
RefSeqNP_001139431.1. NM_001145959.1.
NP_038892.1. NM_013864.2.
XP_006519164.1. XM_006519101.1.
XP_006519165.1. XM_006519102.1.
XP_006519166.1. XM_006519103.1.
XP_006519167.1. XM_006519104.1.
XP_006519168.1. XM_006519105.1.
XP_006534714.1. XM_006534651.1.
XP_006534715.1. XM_006534652.1.
XP_006534716.1. XM_006534653.1.
XP_006534717.1. XM_006534654.1.
XP_006534719.1. XM_006534656.1.
UniGeneMm.26722.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2QMQX-ray1.70A40-313[»]
ProteinModelPortalQ9QYG0.
SMRQ9QYG0. Positions 39-313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9QYG0. 3 interactions.
MINTMINT-1854707.

PTM databases

PhosphoSiteQ9QYG0.

Proteomic databases

PaxDbQ9QYG0.
PRIDEQ9QYG0.

Protocols and materials databases

DNASU29811.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000004673; ENSMUSP00000004673; ENSMUSG00000004558. [Q9QYG0-1]
ENSMUST00000111632; ENSMUSP00000107259; ENSMUSG00000004558. [Q9QYG0-2]
GeneID29811.
KEGGmmu:29811.
UCSCuc007tnk.2. mouse. [Q9QYG0-1]
uc007tnl.2. mouse. [Q9QYG0-2]

Organism-specific databases

CTD57447.
MGIMGI:1352498. Ndrg2.
RougeSearch...

Phylogenomic databases

eggNOGNOG310435.
GeneTreeENSGT00390000001874.
HOGENOMHOG000230891.
HOVERGENHBG052591.
InParanoidQ9QYG0.
OMAQFGDMQE.
OrthoDBEOG7KH9JS.
PhylomeDBQ9QYG0.
TreeFamTF313168.

Gene expression databases

ArrayExpressQ9QYG0.
BgeeQ9QYG0.
CleanExMM_NDRG2.
GenevestigatorQ9QYG0.

Family and domain databases

InterProIPR004142. Ndr.
[Graphical view]
PANTHERPTHR11034. PTHR11034. 1 hit.
PfamPF03096. Ndr. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNDRG2. mouse.
EvolutionaryTraceQ9QYG0.
NextBio306962.
PROQ9QYG0.
SOURCESearch...

Entry information

Entry nameNDRG2_MOUSE
AccessionPrimary (citable) accession number: Q9QYG0
Secondary accession number(s): Q3TI48 expand/collapse secondary AC list , Q3TY42, Q3U3T5, Q69ZN2, Q8C661
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot