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Protein

Pleckstrin homology domain-containing family B member 1

Gene

Plekhb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for proper localization of retinogeniculate projections but not for eye-specific segregation.1 Publication

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Pleckstrin homology domain-containing family B member 1
Short name:
PH domain-containing family B member 1
Alternative name(s):
Evectin-1
PH domain-containing protein in retina 1
Short name:
PHRET1
Pleckstrin homology domain retinal protein 1
Gene namesi
Name:Plekhb1
Synonyms:Evt1, Phr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1351469. Plekhb1.

Subcellular locationi

  • Membrane 1 Publication
  • Cytoplasm 1 Publication

  • Note: Membrane-associated. Highly expressed in the outer segments of photoreceptor cells, both in rods and cones. Localizes to the apical juxta-nuclear Golgi region of the cytoplasm.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB
  • photoreceptor outer segment Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice appear normal at birth with no obvious behavioral or growth abnormalities nor overt sensory deficits. At 6 months and 1 year of age, mice display normal retinal histology and normal response in electroretinograms. Conditional knockout mice, with expression abrogated in the retina, exhibit no gross retinal developmental defects; mutants retain normal retinal lamination, monocular segregation and spontaneous retinal wave activity, but mutant retinal ganglion cells exhibit ipsilateral projection to an improper region of the dorsal lateral geniculate nucleus (dLGN).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243Pleckstrin homology domain-containing family B member 1PRO_0000053887Add
BLAST

Proteomic databases

MaxQBiQ9QYE9.
PaxDbiQ9QYE9.
PRIDEiQ9QYE9.

PTM databases

iPTMnetiQ9QYE9.
PhosphoSiteiQ9QYE9.

Expressioni

Tissue specificityi

Highly expressed in retina and brain. In retina, abundantly expressed in photoreceptors. Isoform 4 is the predominant isoform expressed in mature olfactory receptor neurons and vestibular and cochlear hair cells. Also expressed in cells with possible sensory function, including peripheral retinal ganglion cells, cochlear interdental cells, and neurons of the circumventricular organ (at protein level).3 Publications

Gene expression databases

BgeeiENSMUSG00000030701.
ExpressionAtlasiQ9QYE9. baseline and differential.
GenevisibleiQ9QYE9. MM.

Interactioni

Subunit structurei

Binds transducins (By similarity). Homodimer. Interacts (via PH domain) with MYO1C. Interacts (via PH domain) with MYO7A.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Myo1cQ9WTI74EBI-1127141,EBI-777558

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi205156. 6 interactions.
IntActiQ9QYE9. 2 interactions.
MINTiMINT-1341441.
STRINGi10090.ENSMUSP00000078175.

Structurei

Secondary structure

1
243
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi22 – 3110Combined sources
Beta strandi33 – 353Combined sources
Beta strandi38 – 469Combined sources
Turni47 – 493Combined sources
Beta strandi50 – 589Combined sources
Beta strandi60 – 678Combined sources
Turni69 – 713Combined sources
Beta strandi72 – 776Combined sources
Helixi78 – 803Combined sources
Turni92 – 943Combined sources
Beta strandi95 – 1006Combined sources
Beta strandi105 – 1095Combined sources
Helixi113 – 12715Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D9VNMR-A22-138[»]
ProteinModelPortaliQ9QYE9.
SMRiQ9QYE9. Positions 21-138.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QYE9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 128108PHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IXBE. Eukaryota.
ENOG410Y287. LUCA.
GeneTreeiENSGT00390000013989.
HOGENOMiHOG000253945.
HOVERGENiHBG060487.
InParanoidiQ9QYE9.
OMAiCYSAGAP.
OrthoDBiEOG091G14FB.
PhylomeDBiQ9QYE9.
TreeFamiTF331787.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QYE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSPATPVPPD SILESPFEEM ALVRGGWLWR QSSILRRWKR NWFALWLDGT
60 70 80 90 100
LGYYHDETAQ DEEDRVVIHF NVRDIKVGQE CQDVQPPEGR SRDGLLTVNL
110 120 130 140 150
REGSRLHLCA ETRDDAIAWK TALMEANSTP APAGATVPPR SRRVCPKVRC
160 170 180 190 200
TTLSWNPCKV ERRIWVRVYS PYQDYYEVVP PNAHEATYVR SYYGPPYAGP
210 220 230 240
GVTHVIVRED PCYSSGAPLA MGMLAGAATG AALGSLMWSP CWF
Length:243
Mass (Da):27,340
Last modified:May 1, 2000 - v1
Checksum:i11325285841AF616
GO
Isoform 2 (identifier: Q9QYE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     131-165: Missing.

Show »
Length:208
Mass (Da):23,365
Checksum:i0ABF70948B80769D
GO
Isoform 3 (identifier: Q9QYE9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.

Show »
Length:224
Mass (Da):25,315
Checksum:iD7DEDA160EAF7EB9
GO
Isoform 4 (identifier: Q9QYE9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: Missing.
     131-165: Missing.

Show »
Length:189
Mass (Da):21,340
Checksum:i700BCF1B92FF7072
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1919Missing in isoform 3 and isoform 4. 2 PublicationsVSP_009781Add
BLAST
Alternative sequencei131 – 16535Missing in isoform 2 and isoform 4. 2 PublicationsVSP_009782Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071000 mRNA. Translation: AAD32952.1.
AF000272 mRNA. Translation: AAF16676.1.
AF071001 Genomic DNA. Translation: AAF16683.1. Different termination.
AF101053 mRNA. Translation: AAF18571.1.
AF100613 mRNA. Translation: AAF18933.1.
AK005102 mRNA. Translation: BAB23819.1.
BC024756 mRNA. Translation: AAH24756.1.
CCDSiCCDS21504.1. [Q9QYE9-1]
CCDS52324.1. [Q9QYE9-4]
CCDS52325.1. [Q9QYE9-3]
CCDS52326.1. [Q9QYE9-2]
RefSeqiNP_001156654.1. NM_001163182.1. [Q9QYE9-2]
NP_001156655.1. NM_001163183.1. [Q9QYE9-3]
NP_001156656.1. NM_001163184.1. [Q9QYE9-4]
NP_001156657.1. NM_001163185.1. [Q9QYE9-4]
NP_001156658.1. NM_001163186.1. [Q9QYE9-3]
NP_001156659.1. NM_001163187.1. [Q9QYE9-4]
NP_001278265.1. NM_001291336.1.
NP_038774.1. NM_013746.3. [Q9QYE9-1]
XP_006507969.1. XM_006507906.1. [Q9QYE9-3]
XP_011240128.1. XM_011241826.1. [Q9QYE9-1]
UniGeneiMm.491216.

Genome annotation databases

EnsembliENSMUST00000079176; ENSMUSP00000078175; ENSMUSG00000030701. [Q9QYE9-1]
ENSMUST00000107044; ENSMUSP00000102659; ENSMUSG00000030701. [Q9QYE9-4]
ENSMUST00000107045; ENSMUSP00000102660; ENSMUSG00000030701. [Q9QYE9-3]
ENSMUST00000107046; ENSMUSP00000102661; ENSMUSG00000030701. [Q9QYE9-4]
ENSMUST00000107047; ENSMUSP00000102662; ENSMUSG00000030701. [Q9QYE9-2]
ENSMUST00000116287; ENSMUSP00000111991; ENSMUSG00000030701. [Q9QYE9-3]
GeneIDi27276.
KEGGimmu:27276.
UCSCiuc009ink.2. mouse. [Q9QYE9-1]
uc009ino.2. mouse. [Q9QYE9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF071000 mRNA. Translation: AAD32952.1.
AF000272 mRNA. Translation: AAF16676.1.
AF071001 Genomic DNA. Translation: AAF16683.1. Different termination.
AF101053 mRNA. Translation: AAF18571.1.
AF100613 mRNA. Translation: AAF18933.1.
AK005102 mRNA. Translation: BAB23819.1.
BC024756 mRNA. Translation: AAH24756.1.
CCDSiCCDS21504.1. [Q9QYE9-1]
CCDS52324.1. [Q9QYE9-4]
CCDS52325.1. [Q9QYE9-3]
CCDS52326.1. [Q9QYE9-2]
RefSeqiNP_001156654.1. NM_001163182.1. [Q9QYE9-2]
NP_001156655.1. NM_001163183.1. [Q9QYE9-3]
NP_001156656.1. NM_001163184.1. [Q9QYE9-4]
NP_001156657.1. NM_001163185.1. [Q9QYE9-4]
NP_001156658.1. NM_001163186.1. [Q9QYE9-3]
NP_001156659.1. NM_001163187.1. [Q9QYE9-4]
NP_001278265.1. NM_001291336.1.
NP_038774.1. NM_013746.3. [Q9QYE9-1]
XP_006507969.1. XM_006507906.1. [Q9QYE9-3]
XP_011240128.1. XM_011241826.1. [Q9QYE9-1]
UniGeneiMm.491216.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2D9VNMR-A22-138[»]
ProteinModelPortaliQ9QYE9.
SMRiQ9QYE9. Positions 21-138.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205156. 6 interactions.
IntActiQ9QYE9. 2 interactions.
MINTiMINT-1341441.
STRINGi10090.ENSMUSP00000078175.

PTM databases

iPTMnetiQ9QYE9.
PhosphoSiteiQ9QYE9.

Proteomic databases

MaxQBiQ9QYE9.
PaxDbiQ9QYE9.
PRIDEiQ9QYE9.

Protocols and materials databases

DNASUi27276.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079176; ENSMUSP00000078175; ENSMUSG00000030701. [Q9QYE9-1]
ENSMUST00000107044; ENSMUSP00000102659; ENSMUSG00000030701. [Q9QYE9-4]
ENSMUST00000107045; ENSMUSP00000102660; ENSMUSG00000030701. [Q9QYE9-3]
ENSMUST00000107046; ENSMUSP00000102661; ENSMUSG00000030701. [Q9QYE9-4]
ENSMUST00000107047; ENSMUSP00000102662; ENSMUSG00000030701. [Q9QYE9-2]
ENSMUST00000116287; ENSMUSP00000111991; ENSMUSG00000030701. [Q9QYE9-3]
GeneIDi27276.
KEGGimmu:27276.
UCSCiuc009ink.2. mouse. [Q9QYE9-1]
uc009ino.2. mouse. [Q9QYE9-2]

Organism-specific databases

CTDi58473.
MGIiMGI:1351469. Plekhb1.

Phylogenomic databases

eggNOGiENOG410IXBE. Eukaryota.
ENOG410Y287. LUCA.
GeneTreeiENSGT00390000013989.
HOGENOMiHOG000253945.
HOVERGENiHBG060487.
InParanoidiQ9QYE9.
OMAiCYSAGAP.
OrthoDBiEOG091G14FB.
PhylomeDBiQ9QYE9.
TreeFamiTF331787.

Miscellaneous databases

ChiTaRSiPlekhb1. mouse.
EvolutionaryTraceiQ9QYE9.
PROiQ9QYE9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030701.
ExpressionAtlasiQ9QYE9. baseline and differential.
GenevisibleiQ9QYE9. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKHB1_MOUSE
AccessioniPrimary (citable) accession number: Q9QYE9
Secondary accession number(s): Q9QYB3
, Q9QYB4, Q9QYD2, Q9QYD3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2004
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.