Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Class A basic helix-loop-helix protein 15

Gene

Bhlha15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in controlling the transcriptional activity of MyoD, ensuring that expanding myoblast populations remain undifferentiated (PubMed:17612490). Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'.4 Publications

GO - Molecular functioni

  • DNA binding Source: MGI
  • protein homodimerization activity Source: MGI
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding Source: NTNU_SB

GO - Biological processi

  • calcium-mediated signaling Source: MGI
  • cell-cell signaling Source: MGI
  • cell maturation Source: MGI
  • cellular response to glucose starvation Source: UniProtKB
  • endoplasmic reticulum unfolded protein response Source: UniProtKB
  • glucose homeostasis Source: MGI
  • Golgi organization Source: MGI
  • G-protein coupled receptor signaling pathway Source: MGI
  • intracellular distribution of mitochondria Source: MGI
  • mitochondrial calcium ion transport Source: MGI
  • negative regulation of myotube differentiation Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of transcription, DNA-templated Source: MGI
  • type B pancreatic cell maturation Source: GOC-OWL
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Class A basic helix-loop-helix protein 15
Short name:
bHLHa15
Alternative name(s):
Class B basic helix-loop-helix protein 8
Short name:
bHLHb8
Muscle, intestine and stomach expression 1
Short name:
MIST-1
Gene namesi
Name:Bhlha15
Synonyms:Bhlhb8, Mist1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:891976. Bhlha15.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display incorrect granule organization in pancreatic acinar cells and other serous exocrine cells such as parotid acini and gastric chief cells. They also display mislocalization of mitochondria and Golgi apparatus and reduced Ca2+ uptake by mitochondria.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001271511 – 197Class A basic helix-loop-helix protein 15Add BLAST197

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei12PhosphothreonineCombined sources1
Modified residuei25PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QYC3.
PRIDEiQ9QYC3.

PTM databases

iPTMnetiQ9QYC3.
PhosphoSitePlusiQ9QYC3.

Expressioni

Tissue specificityi

Expressed in pancreatic tissue only in acinar cells. There is a complete absence of expression in intra- or interlobular pancreatic ducts and in all islet cells.1 Publication

Developmental stagei

First observed at E10.5 in the primitive gut and in the developing lung bud. Expression in the gut persists through E16.5 and remains restricted primarily to the epithelial lining of the esophagus, stomach and intestine. Expression in the lung is detected in the bronchial epithelium at E14.5 and at E15.5. Expressed specifically in acinar cells during pancreatic development. Detected in skeletal muscle tissues beginning at E12.5, persisting throughout all embryonic stages examined although, in older embryos expression becomes severely reduced.1 Publication

Inductioni

Up-regulated by XBP1. Induced by chemical activators of the unfolded protein response (UPR) such as tunicamycin and thapsigargin, and also by glucose starvation (PubMed:17612490).

Gene expression databases

BgeeiENSMUSG00000052271.
GenevisibleiQ9QYC3. MM.

Interactioni

Subunit structurei

Forms homodimers or heterodimers with TCF3 gene products E12 and E47. These dimers bind to the E-box site, however, heterodimer with MYOD1 does not bind target DNA.

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

BioGridi201426. 1 interactor.
STRINGi10090.ENSMUSP00000055493.

Structurei

3D structure databases

ProteinModelPortaliQ9QYC3.
SMRiQ9QYC3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini72 – 124bHLHPROSITE-ProRule annotationAdd BLAST53

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi152 – 189Gln-richAdd BLAST38

Domaini

Lacks a classic transcription activation domain and instead possesses an N-terminal region capable of inhibiting heterologous activators.

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410KD2P. Eukaryota.
ENOG4111ZMK. LUCA.
GeneTreeiENSGT00680000099860.
HOGENOMiHOG000095227.
InParanoidiQ9QYC3.
KOiK08040.
OMAiPEPAKGL.
OrthoDBiEOG091G15AI.
PhylomeDBiQ9QYC3.
TreeFamiTF315153.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QYC3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTKNRPPRR RTPMQDTEAT PGEQTPDRPQ SGSGGSELTK GLRSRTARAS
60 70 80 90 100
GGRGEVSRRR QGSGGRRENS VQRRLESNER ERQRMHKLNN AFQALREVIP
110 120 130 140 150
HVRADKKLSK IETLTLAKNY IKSLTATILT MSSSRLPGLE APGPAPGPKL
160 170 180 190
YQHYHHQQQQ QQQQQQVAGA MLGVTEDQPQ GHLQRYSTQI HSFREGS
Length:197
Mass (Da):22,150
Last modified:May 1, 2000 - v1
Checksum:iBB34F1EE76FEE787
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti107Missing in AAF17706 (Ref. 2) Curated1
Sequence conflicti166Q → QV in AAF17706 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091858 Genomic DNA. Translation: AAD51766.1.
AF049660 mRNA. Translation: AAF17706.1.
AK020643 mRNA. Translation: BAB32160.1.
BC011486 mRNA. Translation: AAH11486.1.
CCDSiCCDS19847.1.
RefSeqiNP_034930.1. NM_010800.4.
UniGeneiMm.386767.

Genome annotation databases

EnsembliENSMUST00000060747; ENSMUSP00000055493; ENSMUSG00000052271.
GeneIDi17341.
KEGGimmu:17341.
UCSCiuc009alh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091858 Genomic DNA. Translation: AAD51766.1.
AF049660 mRNA. Translation: AAF17706.1.
AK020643 mRNA. Translation: BAB32160.1.
BC011486 mRNA. Translation: AAH11486.1.
CCDSiCCDS19847.1.
RefSeqiNP_034930.1. NM_010800.4.
UniGeneiMm.386767.

3D structure databases

ProteinModelPortaliQ9QYC3.
SMRiQ9QYC3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201426. 1 interactor.
STRINGi10090.ENSMUSP00000055493.

PTM databases

iPTMnetiQ9QYC3.
PhosphoSitePlusiQ9QYC3.

Proteomic databases

PaxDbiQ9QYC3.
PRIDEiQ9QYC3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060747; ENSMUSP00000055493; ENSMUSG00000052271.
GeneIDi17341.
KEGGimmu:17341.
UCSCiuc009alh.1. mouse.

Organism-specific databases

CTDi168620.
MGIiMGI:891976. Bhlha15.

Phylogenomic databases

eggNOGiENOG410KD2P. Eukaryota.
ENOG4111ZMK. LUCA.
GeneTreeiENSGT00680000099860.
HOGENOMiHOG000095227.
InParanoidiQ9QYC3.
KOiK08040.
OMAiPEPAKGL.
OrthoDBiEOG091G15AI.
PhylomeDBiQ9QYC3.
TreeFamiTF315153.

Miscellaneous databases

PROiQ9QYC3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000052271.
GenevisibleiQ9QYC3. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBHA15_MOUSE
AccessioniPrimary (citable) accession number: Q9QYC3
Secondary accession number(s): Q9QYE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.