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Protein

Alpha-adducin

Gene

Add1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

GO - Molecular functioni

GO - Biological processi

  • actin filament bundle assembly Source: MGI
  • barbed-end actin filament capping Source: MGI
  • cell morphogenesis Source: MGI
  • cell volume homeostasis Source: MGI
  • erythrocyte differentiation Source: MGI
  • hemoglobin metabolic process Source: MGI
  • homeostasis of number of cells within a tissue Source: MGI
  • in utero embryonic development Source: MGI
  • multicellular organism growth Source: MGI
  • positive regulation of protein binding Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-264870. Caspase-mediated cleavage of cytoskeletal proteins.
R-MMU-5223345. Miscellaneous transport and binding events.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-adducin
Alternative name(s):
Erythrocyte adducin subunit alpha
Gene namesi
Name:Add1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:87918. Add1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: MGI
  • F-actin capping protein complex Source: MGI
  • focal adhesion Source: MGI
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 735735Alpha-adducinPRO_0000218531Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei12 – 121PhosphoserineBy similarity
Modified residuei59 – 591PhosphoserineCombined sources
Modified residuei64 – 641PhosphoserineBy similarity
Modified residuei331 – 3311PhosphothreonineBy similarity
Modified residuei334 – 3341PhosphoserineBy similarity
Modified residuei355 – 3551PhosphoserineCombined sources
Modified residuei358 – 3581PhosphothreonineCombined sources
Modified residuei364 – 3641PhosphoserineCombined sources
Modified residuei366 – 3661PhosphoserineCombined sources
Modified residuei408 – 4081PhosphoserineCombined sources
Modified residuei427 – 4271PhosphoserineCombined sources
Modified residuei429 – 4291PhosphothreonineCombined sources
Modified residuei431 – 4311PhosphoserineCombined sources
Modified residuei436 – 4361PhosphoserineCombined sources
Modified residuei445 – 4451Phosphothreonine; by ROCK2By similarity
Modified residuei464 – 4641PhosphoserineCombined sources
Modified residuei465 – 4651PhosphoserineCombined sources
Modified residuei480 – 4801Phosphothreonine; by ROCK2By similarity
Modified residuei481 – 4811Phosphoserine; by PKABy similarity
Modified residuei586 – 5861PhosphoserineCombined sources
Modified residuei600 – 6001PhosphoserineCombined sources
Modified residuei605 – 6051PhosphoserineCombined sources
Modified residuei610 – 6101PhosphothreonineCombined sources
Modified residuei613 – 6131PhosphoserineCombined sources
Modified residuei614 – 6141PhosphothreonineCombined sources
Modified residuei705 – 7051PhosphoserineBy similarity
Modified residuei708 – 7081PhosphoserineBy similarity
Modified residuei712 – 7121PhosphoserineBy similarity
Modified residuei714 – 7141Phosphoserine; by PKCBy similarity
Modified residuei724 – 7241Phosphoserine; by PKA and PKCBy similarity
Isoform 2 (identifier: Q9QYC0-2)
Modified residuei600 – 6001PhosphoserineCombined sources
Modified residuei605 – 6051PhosphoserineCombined sources
Modified residuei610 – 6101PhosphothreonineCombined sources
Modified residuei613 – 6131PhosphoserineCombined sources
Modified residuei614 – 6141PhosphothreonineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9QYC0.
MaxQBiQ9QYC0.
PaxDbiQ9QYC0.
PRIDEiQ9QYC0.

PTM databases

iPTMnetiQ9QYC0.
PhosphoSiteiQ9QYC0.
SwissPalmiQ9QYC0.

Expressioni

Gene expression databases

BgeeiQ9QYC0.
CleanExiMM_ADD1.
ExpressionAtlasiQ9QYC0. baseline and differential.
GenevisibleiQ9QYC0. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi197981. 6 interactions.
IntActiQ9QYC0. 4 interactions.
MINTiMINT-4998369.
STRINGi10090.ENSMUSP00000109979.

Structurei

3D structure databases

ProteinModelPortaliQ9QYC0.
SMRiQ9QYC0. Positions 161-396.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni715 – 73218Interaction with calmodulinSequence analysisAdd
BLAST

Domaini

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
GeneTreeiENSGT00390000016462.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ9QYC0.
KOiK18622.
OMAiTKWTKED.
OrthoDBiEOG7HF1HR.
PhylomeDBiQ9QYC0.
TreeFamiTF313003.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027766. ADD1.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF4. PTHR10672:SF4. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 1 (identifier: Q9QYC0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNGDTRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN
60 70 80 90 100
MMEQKKRVSM ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTA
110 120 130 140 150
SVPNVYPAAP QGGMAALNMS LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA
160 170 180 190 200
AFYRLADLFG WSQLIYNHIT TRVNSEQEHF LIVPFGLLYS EVTASSLVKV
210 220 230 240 250
NLQGDIVDRG STNLGVNQAG FTLHSAVYAA RPDAKCIVHI HTPAGAAVSA
260 270 280 290 300
MKCGLLPISP EALSLGDVAY HDYHGILVDE EEKILIQKNL GPKSKVLILR
310 320 330 340 350
NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLDPGKYK
360 370 380 390 400
AKSRSPGTPA GEGSGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL
410 420 430 440 450
RERSKKYSDV EVPASVTGHS FASDGDSGTC SPLRHSFQKQ QREKTRWLHS
460 470 480 490 500
GRGDDASEEG QNGSSPKSKT KWTKEDGHRT STSAVPNLFV PLNTNPKEVQ
510 520 530 540 550
EMRNKIREQN LQDIKTAGPQ SQVLCGVMMD RSLVQGELVT ASKAIIEKEY
560 570 580 590 600
QPHVIVSTTG PNPFNTLTDR ELEEYRREVE RKQKGSEENL DETREQKEKS
610 620 630 640 650
PPDQSAVPNT PPSTPVKLEE DLPQEPTSRD DSDATTFKPT PPDLSPDEPS
660 670 680 690 700
EALAFPAVEE EAHASPDPTQ PPAEADPEPA SAPTPGAEEV ASPATEEGSP
710 720 730
MDPGSDGSPG KSPSKKKKKF RTPSFLKKSK KKSDS
Length:735
Mass (Da):80,647
Last modified:December 1, 2000 - v2
Checksum:iE6AAA7F6273A33DD
GO
Isoform 2 (identifier: Q9QYC0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     621-632: DLPQEPTSRDDS → AGDGCAKEYLLP
     633-735: Missing.

Show »
Length:632
Mass (Da):69,887
Checksum:iEA5ED051D13C6B38
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti234 – 2341A → T in AAF29504 (PubMed:10845937).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei621 – 63212DLPQE…SRDDS → AGDGCAKEYLLP in isoform 2. CuratedVSP_000177Add
BLAST
Alternative sequencei633 – 735103Missing in isoform 2. CuratedVSP_000178Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096839 mRNA. Translation: AAF24971.1.
AF189771 mRNA. Translation: AAF29504.1.
CCDSiCCDS19216.1. [Q9QYC0-1]
CCDS39069.1. [Q9QYC0-2]
RefSeqiNP_001019629.2. NM_001024458.3. [Q9QYC0-1]
NP_001095914.1. NM_001102444.1.
NP_038485.1. NM_013457.3. [Q9QYC0-2]
UniGeneiMm.289106.

Genome annotation databases

EnsembliENSMUST00000114338; ENSMUSP00000109977; ENSMUSG00000029106. [Q9QYC0-2]
ENSMUST00000114340; ENSMUSP00000109979; ENSMUSG00000029106. [Q9QYC0-1]
GeneIDi11518.
KEGGimmu:11518.
UCSCiuc008xcp.1. mouse. [Q9QYC0-2]
uc008xcr.1. mouse. [Q9QYC0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096839 mRNA. Translation: AAF24971.1.
AF189771 mRNA. Translation: AAF29504.1.
CCDSiCCDS19216.1. [Q9QYC0-1]
CCDS39069.1. [Q9QYC0-2]
RefSeqiNP_001019629.2. NM_001024458.3. [Q9QYC0-1]
NP_001095914.1. NM_001102444.1.
NP_038485.1. NM_013457.3. [Q9QYC0-2]
UniGeneiMm.289106.

3D structure databases

ProteinModelPortaliQ9QYC0.
SMRiQ9QYC0. Positions 161-396.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197981. 6 interactions.
IntActiQ9QYC0. 4 interactions.
MINTiMINT-4998369.
STRINGi10090.ENSMUSP00000109979.

PTM databases

iPTMnetiQ9QYC0.
PhosphoSiteiQ9QYC0.
SwissPalmiQ9QYC0.

Proteomic databases

EPDiQ9QYC0.
MaxQBiQ9QYC0.
PaxDbiQ9QYC0.
PRIDEiQ9QYC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114338; ENSMUSP00000109977; ENSMUSG00000029106. [Q9QYC0-2]
ENSMUST00000114340; ENSMUSP00000109979; ENSMUSG00000029106. [Q9QYC0-1]
GeneIDi11518.
KEGGimmu:11518.
UCSCiuc008xcp.1. mouse. [Q9QYC0-2]
uc008xcr.1. mouse. [Q9QYC0-1]

Organism-specific databases

CTDi118.
MGIiMGI:87918. Add1.

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
GeneTreeiENSGT00390000016462.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ9QYC0.
KOiK18622.
OMAiTKWTKED.
OrthoDBiEOG7HF1HR.
PhylomeDBiQ9QYC0.
TreeFamiTF313003.

Enzyme and pathway databases

ReactomeiR-MMU-264870. Caspase-mediated cleavage of cytoskeletal proteins.
R-MMU-5223345. Miscellaneous transport and binding events.

Miscellaneous databases

ChiTaRSiAdd1. mouse.
NextBioi278944.
PROiQ9QYC0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QYC0.
CleanExiMM_ADD1.
ExpressionAtlasiQ9QYC0. baseline and differential.
GenevisibleiQ9QYC0. MM.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027766. ADD1.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF4. PTHR10672:SF4. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse adducin gene family: alternative splicing and chromosomal localization."
    Suriyapperuma S.P., Lozovatsky L., Ciciotte S.L., Peters L.L., Gilligan D.M.
    Mamm. Genome 11:16-23(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
  2. "Mild spherocytic hereditary elliptocytosis and altered levels of alpha- and gamma-adducins in beta-adducin-deficient mice."
    Muro A.F., Marro M.L., Gajovic S., Porro F., Luzzatto L., Baralle F.E.
    Blood 95:3978-3985(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-610, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-586, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-59; SER-355; THR-358; SER-364; SER-366; SER-408; SER-427; THR-429; SER-431; SER-436; SER-464; SER-465; SER-586; SER-600; SER-605; THR-610; SER-613 AND THR-614, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-600; SER-605; THR-610; SER-613 AND THR-614 (ISOFORM 2), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiADDA_MOUSE
AccessioniPrimary (citable) accession number: Q9QYC0
Secondary accession number(s): Q9JLE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 11, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.