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Q9QYC0

- ADDA_MOUSE

UniProt

Q9QYC0 - ADDA_MOUSE

Protein

Alpha-adducin

Gene

Add1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

    GO - Molecular functioni

    1. structural molecule activity Source: MGI

    GO - Biological processi

    1. actin filament bundle assembly Source: Ensembl
    2. barbed-end actin filament capping Source: Ensembl
    3. cell morphogenesis Source: MGI
    4. cellular response to retinoic acid Source: Ensembl
    5. cell volume homeostasis Source: MGI
    6. erythrocyte differentiation Source: MGI
    7. hemoglobin metabolic process Source: MGI
    8. homeostasis of number of cells within a tissue Source: MGI
    9. in utero embryonic development Source: MGI
    10. multicellular organism growth Source: MGI
    11. positive regulation of angiogenesis Source: Ensembl
    12. positive regulation of endocytosis Source: Ensembl
    13. positive regulation of protein binding Source: Ensembl

    Keywords - Ligandi

    Actin-binding, Calmodulin-binding

    Enzyme and pathway databases

    ReactomeiREACT_106572. XBP1(S) activates chaperone genes.
    REACT_210562. Caspase-mediated cleavage of cytoskeletal proteins.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-adducin
    Alternative name(s):
    Erythrocyte adducin subunit alpha
    Gene namesi
    Name:Add1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:87918. Add1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoskeleton Source: MGI
    2. dendritic spine Source: Ensembl
    3. F-actin capping protein complex Source: Ensembl
    4. membrane Source: MGI
    5. nucleus Source: Ensembl
    6. perinuclear region of cytoplasm Source: Ensembl
    7. plasma membrane Source: UniProtKB-SubCell
    8. postsynaptic density Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Cytoskeleton, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 735735Alpha-adducinPRO_0000218531Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei12 – 121PhosphoserineBy similarity
    Modified residuei59 – 591Phosphoserine; by PKABy similarity
    Modified residuei331 – 3311PhosphothreonineBy similarity
    Modified residuei334 – 3341PhosphoserineBy similarity
    Modified residuei408 – 4081Phosphoserine; by PKABy similarity
    Modified residuei431 – 4311PhosphoserineBy similarity
    Modified residuei436 – 4361Phosphoserine; by PKABy similarity
    Modified residuei445 – 4451Phosphothreonine; by ROCK2By similarity
    Modified residuei465 – 4651PhosphoserineBy similarity
    Modified residuei480 – 4801Phosphothreonine; by ROCK2By similarity
    Modified residuei481 – 4811Phosphoserine; by PKABy similarity
    Modified residuei586 – 5861Phosphoserine1 Publication
    Modified residuei610 – 6101Phosphothreonine1 Publication
    Modified residuei705 – 7051PhosphoserineBy similarity
    Modified residuei708 – 7081PhosphoserineBy similarity
    Modified residuei712 – 7121PhosphoserineBy similarity
    Modified residuei714 – 7141Phosphoserine; by PKCBy similarity
    Modified residuei724 – 7241Phosphoserine; by PKA and PKCBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ9QYC0.
    PaxDbiQ9QYC0.
    PRIDEiQ9QYC0.

    PTM databases

    PhosphoSiteiQ9QYC0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9QYC0.
    BgeeiQ9QYC0.
    CleanExiMM_ADD1.
    GenevestigatoriQ9QYC0.

    Interactioni

    Subunit structurei

    Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.

    Protein-protein interaction databases

    BioGridi197981. 6 interactions.
    IntActiQ9QYC0. 4 interactions.
    MINTiMINT-4998369.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QYC0.
    SMRiQ9QYC0. Positions 161-396.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni715 – 73218Interaction with calmodulinSequence AnalysisAdd
    BLAST

    Domaini

    Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0235.
    GeneTreeiENSGT00390000016462.
    HOGENOMiHOG000116349.
    HOVERGENiHBG004180.
    InParanoidiQ9QYC0.
    OMAiWTKEDGH.
    OrthoDBiEOG7HF1HR.
    PhylomeDBiQ9QYC0.
    TreeFamiTF313003.

    Family and domain databases

    Gene3Di3.40.225.10. 1 hit.
    InterProiIPR027766. ADD1.
    IPR001303. Aldolase_II/adducin_N.
    [Graphical view]
    PANTHERiPTHR10672:SF4. PTHR10672:SF4. 1 hit.
    PfamiPF00596. Aldolase_II. 1 hit.
    [Graphical view]
    SMARTiSM01007. Aldolase_II. 1 hit.
    [Graphical view]
    SUPFAMiSSF53639. SSF53639. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform 1 (identifier: Q9QYC0-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNGDTRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN    50
    MMEQKKRVSM ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTA 100
    SVPNVYPAAP QGGMAALNMS LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA 150
    AFYRLADLFG WSQLIYNHIT TRVNSEQEHF LIVPFGLLYS EVTASSLVKV 200
    NLQGDIVDRG STNLGVNQAG FTLHSAVYAA RPDAKCIVHI HTPAGAAVSA 250
    MKCGLLPISP EALSLGDVAY HDYHGILVDE EEKILIQKNL GPKSKVLILR 300
    NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLDPGKYK 350
    AKSRSPGTPA GEGSGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL 400
    RERSKKYSDV EVPASVTGHS FASDGDSGTC SPLRHSFQKQ QREKTRWLHS 450
    GRGDDASEEG QNGSSPKSKT KWTKEDGHRT STSAVPNLFV PLNTNPKEVQ 500
    EMRNKIREQN LQDIKTAGPQ SQVLCGVMMD RSLVQGELVT ASKAIIEKEY 550
    QPHVIVSTTG PNPFNTLTDR ELEEYRREVE RKQKGSEENL DETREQKEKS 600
    PPDQSAVPNT PPSTPVKLEE DLPQEPTSRD DSDATTFKPT PPDLSPDEPS 650
    EALAFPAVEE EAHASPDPTQ PPAEADPEPA SAPTPGAEEV ASPATEEGSP 700
    MDPGSDGSPG KSPSKKKKKF RTPSFLKKSK KKSDS 735
    Length:735
    Mass (Da):80,647
    Last modified:December 1, 2000 - v2
    Checksum:iE6AAA7F6273A33DD
    GO
    Isoform 2 (identifier: Q9QYC0-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         621-632: DLPQEPTSRDDS → AGDGCAKEYLLP
         633-735: Missing.

    Show »
    Length:632
    Mass (Da):69,887
    Checksum:iEA5ED051D13C6B38
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti234 – 2341A → T in AAF29504. (PubMed:10845937)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei621 – 63212DLPQE…SRDDS → AGDGCAKEYLLP in isoform 2. CuratedVSP_000177Add
    BLAST
    Alternative sequencei633 – 735103Missing in isoform 2. CuratedVSP_000178Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF096839 mRNA. Translation: AAF24971.1.
    AF189771 mRNA. Translation: AAF29504.1.
    CCDSiCCDS19216.1. [Q9QYC0-1]
    CCDS39069.1. [Q9QYC0-2]
    RefSeqiNP_001019629.2. NM_001024458.3. [Q9QYC0-1]
    NP_001095914.1. NM_001102444.1.
    NP_038485.1. NM_013457.3. [Q9QYC0-2]
    UniGeneiMm.289106.

    Genome annotation databases

    EnsembliENSMUST00000114338; ENSMUSP00000109977; ENSMUSG00000029106. [Q9QYC0-2]
    ENSMUST00000114340; ENSMUSP00000109979; ENSMUSG00000029106. [Q9QYC0-1]
    GeneIDi11518.
    KEGGimmu:11518.
    UCSCiuc008xcp.1. mouse. [Q9QYC0-2]
    uc008xcr.1. mouse. [Q9QYC0-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF096839 mRNA. Translation: AAF24971.1 .
    AF189771 mRNA. Translation: AAF29504.1 .
    CCDSi CCDS19216.1. [Q9QYC0-1 ]
    CCDS39069.1. [Q9QYC0-2 ]
    RefSeqi NP_001019629.2. NM_001024458.3. [Q9QYC0-1 ]
    NP_001095914.1. NM_001102444.1.
    NP_038485.1. NM_013457.3. [Q9QYC0-2 ]
    UniGenei Mm.289106.

    3D structure databases

    ProteinModelPortali Q9QYC0.
    SMRi Q9QYC0. Positions 161-396.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 197981. 6 interactions.
    IntActi Q9QYC0. 4 interactions.
    MINTi MINT-4998369.

    PTM databases

    PhosphoSitei Q9QYC0.

    Proteomic databases

    MaxQBi Q9QYC0.
    PaxDbi Q9QYC0.
    PRIDEi Q9QYC0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000114338 ; ENSMUSP00000109977 ; ENSMUSG00000029106 . [Q9QYC0-2 ]
    ENSMUST00000114340 ; ENSMUSP00000109979 ; ENSMUSG00000029106 . [Q9QYC0-1 ]
    GeneIDi 11518.
    KEGGi mmu:11518.
    UCSCi uc008xcp.1. mouse. [Q9QYC0-2 ]
    uc008xcr.1. mouse. [Q9QYC0-1 ]

    Organism-specific databases

    CTDi 118.
    MGIi MGI:87918. Add1.

    Phylogenomic databases

    eggNOGi COG0235.
    GeneTreei ENSGT00390000016462.
    HOGENOMi HOG000116349.
    HOVERGENi HBG004180.
    InParanoidi Q9QYC0.
    OMAi WTKEDGH.
    OrthoDBi EOG7HF1HR.
    PhylomeDBi Q9QYC0.
    TreeFami TF313003.

    Enzyme and pathway databases

    Reactomei REACT_106572. XBP1(S) activates chaperone genes.
    REACT_210562. Caspase-mediated cleavage of cytoskeletal proteins.

    Miscellaneous databases

    ChiTaRSi ADD1. mouse.
    NextBioi 278944.
    PROi Q9QYC0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9QYC0.
    Bgeei Q9QYC0.
    CleanExi MM_ADD1.
    Genevestigatori Q9QYC0.

    Family and domain databases

    Gene3Di 3.40.225.10. 1 hit.
    InterProi IPR027766. ADD1.
    IPR001303. Aldolase_II/adducin_N.
    [Graphical view ]
    PANTHERi PTHR10672:SF4. PTHR10672:SF4. 1 hit.
    Pfami PF00596. Aldolase_II. 1 hit.
    [Graphical view ]
    SMARTi SM01007. Aldolase_II. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53639. SSF53639. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The mouse adducin gene family: alternative splicing and chromosomal localization."
      Suriyapperuma S.P., Lozovatsky L., Ciciotte S.L., Peters L.L., Gilligan D.M.
      Mamm. Genome 11:16-23(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    2. "Mild spherocytic hereditary elliptocytosis and altered levels of alpha- and gamma-adducins in beta-adducin-deficient mice."
      Muro A.F., Marro M.L., Gajovic S., Porro F., Luzzatto L., Baralle F.E.
      Blood 95:3978-3985(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-610, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic brain.
    4. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
      Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
      Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-586, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiADDA_MOUSE
    AccessioniPrimary (citable) accession number: Q9QYC0
    Secondary accession number(s): Q9JLE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3