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Protein

Alpha-adducin

Gene

Add1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

GO - Molecular functioni

GO - Biological processi

  • actin filament bundle assembly Source: MGI
  • barbed-end actin filament capping Source: MGI
  • cell morphogenesis Source: MGI
  • cell volume homeostasis Source: MGI
  • erythrocyte differentiation Source: MGI
  • hemoglobin metabolic process Source: MGI
  • homeostasis of number of cells within a tissue Source: MGI
  • in utero embryonic development Source: MGI
  • multicellular organism growth Source: MGI
  • positive regulation of protein binding Source: MGI
Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-264870. Caspase-mediated cleavage of cytoskeletal proteins.
R-MMU-5223345. Miscellaneous transport and binding events.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-adducin
Alternative name(s):
Erythrocyte adducin subunit alpha
Gene namesi
Name:Add1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:87918. Add1.

Subcellular locationi

GO - Cellular componenti

  • cell-cell adherens junction Source: MGI
  • cytoplasm Source: UniProtKB-KW
  • cytoskeleton Source: MGI
  • F-actin capping protein complex Source: MGI
  • focal adhesion Source: MGI
  • membrane Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002185311 – 735Alpha-adducinAdd BLAST735

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei12PhosphoserineBy similarity1
Modified residuei59PhosphoserineCombined sources1
Modified residuei64PhosphoserineBy similarity1
Modified residuei331PhosphothreonineBy similarity1
Modified residuei334PhosphoserineBy similarity1
Modified residuei353PhosphoserineBy similarity1
Modified residuei355PhosphoserineCombined sources1
Modified residuei358PhosphothreonineCombined sources1
Modified residuei364PhosphoserineCombined sources1
Modified residuei366PhosphoserineCombined sources1
Modified residuei408PhosphoserineCombined sources1
Modified residuei427PhosphoserineCombined sources1
Modified residuei429PhosphothreonineCombined sources1
Modified residuei431PhosphoserineCombined sources1
Modified residuei436PhosphoserineCombined sources1
Modified residuei445Phosphothreonine; by ROCK2By similarity1
Modified residuei464PhosphoserineCombined sources1
Modified residuei465PhosphoserineCombined sources1
Modified residuei480Phosphothreonine; by ROCK2By similarity1
Modified residuei481Phosphoserine; by PKABy similarity1
Modified residuei586PhosphoserineCombined sources1
Modified residuei600PhosphoserineCombined sources1
Modified residuei605PhosphoserineCombined sources1
Modified residuei610PhosphothreonineCombined sources1
Modified residuei613PhosphoserineCombined sources1
Modified residuei614PhosphothreonineCombined sources1
Modified residuei705PhosphoserineBy similarity1
Modified residuei708PhosphoserineBy similarity1
Modified residuei712PhosphoserineBy similarity1
Modified residuei714Phosphoserine; by PKCBy similarity1
Modified residuei724Phosphoserine; by PKA and PKCBy similarity1
Isoform 2 (identifier: Q9QYC0-2)
Modified residuei600PhosphoserineCombined sources1
Modified residuei605PhosphoserineCombined sources1
Modified residuei610PhosphothreonineCombined sources1
Modified residuei613PhosphoserineCombined sources1
Modified residuei614PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9QYC0.
PaxDbiQ9QYC0.
PeptideAtlasiQ9QYC0.
PRIDEiQ9QYC0.

PTM databases

iPTMnetiQ9QYC0.
PhosphoSitePlusiQ9QYC0.
SwissPalmiQ9QYC0.

Expressioni

Gene expression databases

BgeeiENSMUSG00000029106.
CleanExiMM_ADD1.
ExpressionAtlasiQ9QYC0. baseline and differential.
GenevisibleiQ9QYC0. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi197981. 6 interactors.
IntActiQ9QYC0. 4 interactors.
MINTiMINT-4998369.
STRINGi10090.ENSMUSP00000109979.

Structurei

3D structure databases

ProteinModelPortaliQ9QYC0.
SMRiQ9QYC0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni715 – 732Interaction with calmodulinSequence analysisAdd BLAST18

Domaini

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
GeneTreeiENSGT00390000016462.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ9QYC0.
KOiK18622.
OMAiVNSEQEH.
OrthoDBiEOG091G0324.
PhylomeDBiQ9QYC0.
TreeFamiTF313003.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027766. ADD1.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF4. PTHR10672:SF4. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q9QYC0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNGDTRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN
60 70 80 90 100
MMEQKKRVSM ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTA
110 120 130 140 150
SVPNVYPAAP QGGMAALNMS LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA
160 170 180 190 200
AFYRLADLFG WSQLIYNHIT TRVNSEQEHF LIVPFGLLYS EVTASSLVKV
210 220 230 240 250
NLQGDIVDRG STNLGVNQAG FTLHSAVYAA RPDAKCIVHI HTPAGAAVSA
260 270 280 290 300
MKCGLLPISP EALSLGDVAY HDYHGILVDE EEKILIQKNL GPKSKVLILR
310 320 330 340 350
NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLDPGKYK
360 370 380 390 400
AKSRSPGTPA GEGSGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL
410 420 430 440 450
RERSKKYSDV EVPASVTGHS FASDGDSGTC SPLRHSFQKQ QREKTRWLHS
460 470 480 490 500
GRGDDASEEG QNGSSPKSKT KWTKEDGHRT STSAVPNLFV PLNTNPKEVQ
510 520 530 540 550
EMRNKIREQN LQDIKTAGPQ SQVLCGVMMD RSLVQGELVT ASKAIIEKEY
560 570 580 590 600
QPHVIVSTTG PNPFNTLTDR ELEEYRREVE RKQKGSEENL DETREQKEKS
610 620 630 640 650
PPDQSAVPNT PPSTPVKLEE DLPQEPTSRD DSDATTFKPT PPDLSPDEPS
660 670 680 690 700
EALAFPAVEE EAHASPDPTQ PPAEADPEPA SAPTPGAEEV ASPATEEGSP
710 720 730
MDPGSDGSPG KSPSKKKKKF RTPSFLKKSK KKSDS
Length:735
Mass (Da):80,647
Last modified:December 1, 2000 - v2
Checksum:iE6AAA7F6273A33DD
GO
Isoform 2 (identifier: Q9QYC0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     621-632: DLPQEPTSRDDS → AGDGCAKEYLLP
     633-735: Missing.

Show »
Length:632
Mass (Da):69,887
Checksum:iEA5ED051D13C6B38
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti234A → T in AAF29504 (PubMed:10845937).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_000177621 – 632DLPQE…SRDDS → AGDGCAKEYLLP in isoform 2. CuratedAdd BLAST12
Alternative sequenceiVSP_000178633 – 735Missing in isoform 2. CuratedAdd BLAST103

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096839 mRNA. Translation: AAF24971.1.
AF189771 mRNA. Translation: AAF29504.1.
CCDSiCCDS19216.1. [Q9QYC0-1]
CCDS39069.1. [Q9QYC0-2]
RefSeqiNP_001019629.2. NM_001024458.4. [Q9QYC0-1]
NP_038485.1. NM_013457.4. [Q9QYC0-2]
XP_017176104.1. XM_017320615.1. [Q9QYC0-2]
UniGeneiMm.289106.

Genome annotation databases

EnsembliENSMUST00000114338; ENSMUSP00000109977; ENSMUSG00000029106. [Q9QYC0-2]
ENSMUST00000114340; ENSMUSP00000109979; ENSMUSG00000029106. [Q9QYC0-1]
GeneIDi11518.
KEGGimmu:11518.
UCSCiuc008xcp.1. mouse. [Q9QYC0-2]
uc008xcr.1. mouse. [Q9QYC0-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF096839 mRNA. Translation: AAF24971.1.
AF189771 mRNA. Translation: AAF29504.1.
CCDSiCCDS19216.1. [Q9QYC0-1]
CCDS39069.1. [Q9QYC0-2]
RefSeqiNP_001019629.2. NM_001024458.4. [Q9QYC0-1]
NP_038485.1. NM_013457.4. [Q9QYC0-2]
XP_017176104.1. XM_017320615.1. [Q9QYC0-2]
UniGeneiMm.289106.

3D structure databases

ProteinModelPortaliQ9QYC0.
SMRiQ9QYC0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197981. 6 interactors.
IntActiQ9QYC0. 4 interactors.
MINTiMINT-4998369.
STRINGi10090.ENSMUSP00000109979.

PTM databases

iPTMnetiQ9QYC0.
PhosphoSitePlusiQ9QYC0.
SwissPalmiQ9QYC0.

Proteomic databases

EPDiQ9QYC0.
PaxDbiQ9QYC0.
PeptideAtlasiQ9QYC0.
PRIDEiQ9QYC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114338; ENSMUSP00000109977; ENSMUSG00000029106. [Q9QYC0-2]
ENSMUST00000114340; ENSMUSP00000109979; ENSMUSG00000029106. [Q9QYC0-1]
GeneIDi11518.
KEGGimmu:11518.
UCSCiuc008xcp.1. mouse. [Q9QYC0-2]
uc008xcr.1. mouse. [Q9QYC0-1]

Organism-specific databases

CTDi118.
MGIiMGI:87918. Add1.

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
GeneTreeiENSGT00390000016462.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ9QYC0.
KOiK18622.
OMAiVNSEQEH.
OrthoDBiEOG091G0324.
PhylomeDBiQ9QYC0.
TreeFamiTF313003.

Enzyme and pathway databases

ReactomeiR-MMU-264870. Caspase-mediated cleavage of cytoskeletal proteins.
R-MMU-5223345. Miscellaneous transport and binding events.

Miscellaneous databases

ChiTaRSiAdd1. mouse.
PROiQ9QYC0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029106.
CleanExiMM_ADD1.
ExpressionAtlasiQ9QYC0. baseline and differential.
GenevisibleiQ9QYC0. MM.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027766. ADD1.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF4. PTHR10672:SF4. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADDA_MOUSE
AccessioniPrimary (citable) accession number: Q9QYC0
Secondary accession number(s): Q9JLE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.