Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9QYC0 (ADDA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-adducin
Alternative name(s):
Erythrocyte adducin subunit alpha
Gene names
Name:Add1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length735 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Subunit structure

Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.

Subcellular location

Cytoplasmcytoskeleton. Cell membrane; Peripheral membrane protein; Cytoplasmic side.

Domain

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Sequence similarities

Belongs to the aldolase class II family. Adducin subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Cytoplasm
Cytoskeleton
Membrane
   Coding sequence diversityAlternative splicing
   LigandActin-binding
Calmodulin-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin filament bundle assembly

Inferred from electronic annotation. Source: Ensembl

barbed-end actin filament capping

Inferred from electronic annotation. Source: Ensembl

cell morphogenesis

Inferred from mutant phenotype PubMed 18723693. Source: MGI

cell volume homeostasis

Inferred from mutant phenotype PubMed 18723693. Source: MGI

cellular response to retinoic acid

Inferred from electronic annotation. Source: Ensembl

erythrocyte differentiation

Inferred from mutant phenotype PubMed 18723693. Source: MGI

hemoglobin metabolic process

Inferred from mutant phenotype PubMed 18723693. Source: MGI

homeostasis of number of cells within a tissue

Inferred from mutant phenotype PubMed 18723693. Source: MGI

in utero embryonic development

Inferred from mutant phenotype PubMed 18723693. Source: MGI

multicellular organism growth

Inferred from mutant phenotype PubMed 18723693. Source: MGI

positive regulation of angiogenesis

Inferred from electronic annotation. Source: Ensembl

positive regulation of endocytosis

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein binding

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentF-actin capping protein complex

Inferred from electronic annotation. Source: Ensembl

cytoskeleton

Inferred from direct assay PubMed 10485892. Source: MGI

dendritic spine

Inferred from electronic annotation. Source: Ensembl

membrane

Inferred from direct assay PubMed 10485892. Source: MGI

nucleus

Inferred from electronic annotation. Source: Ensembl

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

postsynaptic density

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionstructural molecule activity

Inferred from direct assay PubMed 10485892. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform 1 (identifier: Q9QYC0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9QYC0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     621-632: DLPQEPTSRDDS → AGDGCAKEYLLP
     633-735: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 735735Alpha-adducin
PRO_0000218531

Regions

Region715 – 73218Interaction with calmodulin Potential

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue121Phosphoserine By similarity
Modified residue591Phosphoserine; by PKA By similarity
Modified residue3311Phosphothreonine By similarity
Modified residue3341Phosphoserine By similarity
Modified residue4081Phosphoserine; by PKA By similarity
Modified residue4311Phosphoserine By similarity
Modified residue4361Phosphoserine; by PKA By similarity
Modified residue4451Phosphothreonine; by ROCK2 By similarity
Modified residue4651Phosphoserine By similarity
Modified residue4801Phosphothreonine; by ROCK2 By similarity
Modified residue4811Phosphoserine; by PKA By similarity
Modified residue5861Phosphoserine Ref.4
Modified residue6101Phosphothreonine Ref.3
Modified residue7051Phosphoserine By similarity
Modified residue7081Phosphoserine By similarity
Modified residue7121Phosphoserine By similarity
Modified residue7141Phosphoserine; by PKC By similarity
Modified residue7241Phosphoserine; by PKA and PKC By similarity

Natural variations

Alternative sequence621 – 63212DLPQE…SRDDS → AGDGCAKEYLLP in isoform 2.
VSP_000177
Alternative sequence633 – 735103Missing in isoform 2.
VSP_000178

Experimental info

Sequence conflict2341A → T in AAF29504. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: E6AAA7F6273A33DD

FASTA73580,647
        10         20         30         40         50         60 
MNGDTRAAVV TSPPPTTAPH KERYFDRVDE NNPEYLRERN MAPDLRQDFN MMEQKKRVSM 

        70         80         90        100        110        120 
ILQSPAFCEE LESMIQEQFK KGKNPTGLLA LQQIADFMTA SVPNVYPAAP QGGMAALNMS 

       130        140        150        160        170        180 
LGMVTPVNDL RGSDSIAYDK GEKLLRCKLA AFYRLADLFG WSQLIYNHIT TRVNSEQEHF 

       190        200        210        220        230        240 
LIVPFGLLYS EVTASSLVKV NLQGDIVDRG STNLGVNQAG FTLHSAVYAA RPDAKCIVHI 

       250        260        270        280        290        300 
HTPAGAAVSA MKCGLLPISP EALSLGDVAY HDYHGILVDE EEKILIQKNL GPKSKVLILR 

       310        320        330        340        350        360 
NHGLVSVGES VEEAFYYIHN LVVACEIQVR TLASAGGPDN LVLLDPGKYK AKSRSPGTPA 

       370        380        390        400        410        420 
GEGSGSPPKW QIGEQEFEAL MRMLDNLGYR TGYPYRYPAL RERSKKYSDV EVPASVTGHS 

       430        440        450        460        470        480 
FASDGDSGTC SPLRHSFQKQ QREKTRWLHS GRGDDASEEG QNGSSPKSKT KWTKEDGHRT 

       490        500        510        520        530        540 
STSAVPNLFV PLNTNPKEVQ EMRNKIREQN LQDIKTAGPQ SQVLCGVMMD RSLVQGELVT 

       550        560        570        580        590        600 
ASKAIIEKEY QPHVIVSTTG PNPFNTLTDR ELEEYRREVE RKQKGSEENL DETREQKEKS 

       610        620        630        640        650        660 
PPDQSAVPNT PPSTPVKLEE DLPQEPTSRD DSDATTFKPT PPDLSPDEPS EALAFPAVEE 

       670        680        690        700        710        720 
EAHASPDPTQ PPAEADPEPA SAPTPGAEEV ASPATEEGSP MDPGSDGSPG KSPSKKKKKF 

       730 
RTPSFLKKSK KKSDS 

« Hide

Isoform 2 [UniParc].

Checksum: EA5ED051D13C6B38
Show »

FASTA63269,887

References

« Hide 'large scale' references
[1]"The mouse adducin gene family: alternative splicing and chromosomal localization."
Suriyapperuma S.P., Lozovatsky L., Ciciotte S.L., Peters L.L., Gilligan D.M.
Mamm. Genome 11:16-23(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[2]"Mild spherocytic hereditary elliptocytosis and altered levels of alpha- and gamma-adducins in beta-adducin-deficient mice."
Muro A.F., Marro M.L., Gajovic S., Porro F., Luzzatto L., Baralle F.E.
Blood 95:3978-3985(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[3]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-610, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic brain.
[4]"Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-586, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Brain.
[5]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF096839 mRNA. Translation: AAF24971.1.
AF189771 mRNA. Translation: AAF29504.1.
CCDSCCDS19216.1. [Q9QYC0-1]
CCDS39069.1. [Q9QYC0-2]
RefSeqNP_001019629.2. NM_001024458.3. [Q9QYC0-1]
NP_001095914.1. NM_001102444.1.
NP_038485.1. NM_013457.3. [Q9QYC0-2]
UniGeneMm.289106.

3D structure databases

ProteinModelPortalQ9QYC0.
SMRQ9QYC0. Positions 161-396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid197981. 6 interactions.
IntActQ9QYC0. 4 interactions.
MINTMINT-4998369.

PTM databases

PhosphoSiteQ9QYC0.

Proteomic databases

MaxQBQ9QYC0.
PaxDbQ9QYC0.
PRIDEQ9QYC0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000114338; ENSMUSP00000109977; ENSMUSG00000029106. [Q9QYC0-2]
ENSMUST00000114340; ENSMUSP00000109979; ENSMUSG00000029106. [Q9QYC0-1]
GeneID11518.
KEGGmmu:11518.
UCSCuc008xcp.1. mouse. [Q9QYC0-2]
uc008xcr.1. mouse. [Q9QYC0-1]

Organism-specific databases

CTD118.
MGIMGI:87918. Add1.

Phylogenomic databases

eggNOGCOG0235.
GeneTreeENSGT00390000016462.
HOGENOMHOG000116349.
HOVERGENHBG004180.
InParanoidQ9QYC0.
OMAWTKEDGH.
OrthoDBEOG7HF1HR.
PhylomeDBQ9QYC0.
TreeFamTF313003.

Gene expression databases

ArrayExpressQ9QYC0.
BgeeQ9QYC0.
CleanExMM_ADD1.
GenevestigatorQ9QYC0.

Family and domain databases

Gene3D3.40.225.10. 1 hit.
InterProIPR027766. ADD1.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERPTHR10672:SF4. PTHR10672:SF4. 1 hit.
PfamPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMSSF53639. SSF53639. 1 hit.
ProtoNetSearch...

Other

ChiTaRSADD1. mouse.
NextBio278944.
PROQ9QYC0.
SOURCESearch...

Entry information

Entry nameADDA_MOUSE
AccessionPrimary (citable) accession number: Q9QYC0
Secondary accession number(s): Q9JLE3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot