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Protein

Gamma-adducin

Gene

Add3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

GO - Molecular functioni

  • structural molecule activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Actin-binding, Calmodulin-binding

Enzyme and pathway databases

ReactomeiR-MMU-5223345. Miscellaneous transport and binding events.

Names & Taxonomyi

Protein namesi
Recommended name:
Gamma-adducin
Alternative name(s):
Adducin-like protein 70
Gene namesi
Name:Add3
Synonyms:Addl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1351615. Add3.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: UniProtKB
  • cell-cell junction Source: MGI
  • cell cortex Source: MGI
  • condensed nuclear chromosome Source: MGI
  • cytoplasm Source: MGI
  • cytoskeleton Source: MGI
  • membrane Source: MGI
  • neuronal postsynaptic density Source: MGI
  • nucleoplasm Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 706705Gamma-adducinPRO_0000218537Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity
Modified residuei31 – 311PhosphoserineCombined sources
Modified residuei42 – 421PhosphoserineCombined sources
Modified residuei64 – 641PhosphoserineCombined sources
Modified residuei402 – 4021PhosphoserineCombined sources
Modified residuei414 – 4141PhosphoserineCombined sources
Modified residuei423 – 4231PhosphoserineCombined sources
Modified residuei442 – 4421PhosphoserineBy similarity
Modified residuei583 – 5831PhosphoserineCombined sources
Modified residuei585 – 5851PhosphoserineCombined sources
Modified residuei590 – 5901PhosphoserineCombined sources
Modified residuei673 – 6731PhosphoserineCombined sources
Modified residuei677 – 6771PhosphoserineCombined sources
Modified residuei679 – 6791PhosphoserineCombined sources
Modified residuei681 – 6811PhosphoserineCombined sources
Modified residuei683 – 6831PhosphoserineCombined sources

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9QYB5.
MaxQBiQ9QYB5.
PaxDbiQ9QYB5.
PRIDEiQ9QYB5.

PTM databases

iPTMnetiQ9QYB5.
PhosphoSiteiQ9QYB5.

Expressioni

Gene expression databases

BgeeiQ9QYB5.
CleanExiMM_ADD3.
ExpressionAtlasiQ9QYB5. baseline and differential.
GenevisibleiQ9QYB5. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a gamma subunit.

Protein-protein interaction databases

BioGridi205169. 1 interaction.
IntActiQ9QYB5. 2 interactions.
MINTiMINT-4087243.
STRINGi10090.ENSMUSP00000025999.

Structurei

3D structure databases

ProteinModelPortaliQ9QYB5.
SMRiQ9QYB5. Positions 138-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni684 – 70118Interaction with calmodulinSequence analysisAdd
BLAST

Domaini

Comprised of three regions: a N-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
GeneTreeiENSGT00390000016462.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ9QYB5.
KOiK18622.
OMAiRTKITWM.
OrthoDBiEOG7HF1HR.
TreeFamiTF313003.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027772. ADD3.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF5. PTHR10672:SF5. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform 2 (identifier: Q9QYB5-1) [UniParc]FASTAAdd to basket

Also known as: Long

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSDTSPAVV TTPPPPSMPH KERYFDRINE SDPEYLRERN MSPDLRQDFN
60 70 80 90 100
MMEQRKRVTQ ILQSPAFRED LECLIQEQMK KGHNPSGLLA LQQIADYIVT
110 120 130 140 150
SSFSGFSSPS LSLGMVTPIN DLPGADTSSY VKGEKLTRCK LASLYRLADL
160 170 180 190 200
FGWAHLANTY ISVRISKEQD HIIIIPRGLS FSEATASTLV KVNIIGEVVD
210 220 230 240 250
QGSTDLKIDH TGFSPHAAIY STRPDVKCVI HIHTLATAAV SSMKCGILPI
260 270 280 290 300
SQESLILGDV AYYDYQGSLD EEEERIELQK VLGPSCKVLV LRNHGMVALG
310 320 330 340 350
ETLEEAFHYI FNVQMACEIQ VQAVAGAGGV DNLLVLDLQK YKAFTHGVAM
360 370 380 390 400
SGGGGVNMAS HQKWKVGEIE FEGLMRTLDN LGYRTGYAYR HPLVREKPRH
410 420 430 440 450
KSDVEIPATV TAFSFEDDSA PLSPLKFMAQ RQQREKTRWL NSPNTYMKVN
460 470 480 490 500
VPEESRNGET SPRTKITWMK AEDSSKVSSG TPIKIEDPNQ FVPLNTNPTE
510 520 530 540 550
VLEKRNKIRE QNRYDLKTAG PQSQLLAGIV VDKPPSTMQF DDDDQGPPAP
560 570 580 590 600
PNPFSHLLEG ELEEYTKTIE RKQQGLDDAE QGSLSDDAAS VSQIQSQTQS
610 620 630 640 650
PQSVPERLEE NHELFSKSFT SMDAPVMIMN GKDEMHDVED ELAQRVSRLT
660 670 680 690 700
TSTTIENIEI TIKSPERTEE VLSPDGSPSK SPSKKKKKFR TPSFLKKNKK

KEKVEA
Length:706
Mass (Da):78,777
Last modified:June 28, 2011 - v2
Checksum:i3866D14F33D262E4
GO
Isoform 1 (identifier: Q9QYB5-2) [UniParc]FASTAAdd to basket

Also known as: Short

The sequence of this isoform differs from the canonical sequence as follows:
     578-609: Missing.

Show »
Length:674
Mass (Da):75,407
Checksum:iDE6D2EE55F7A1E8D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371R → G in BAB31757 (PubMed:16141072).Curated
Sequence conflicti377 – 3771T → S in AAF24974 (PubMed:10602987).Curated
Sequence conflicti377 – 3771T → S in AAF24975 (PubMed:10602987).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei578 – 60932Missing in isoform 1. CuratedVSP_000189Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100424 mRNA. Translation: AAF24974.1.
AF100425 mRNA. Translation: AAF24975.1.
DQ479918 Genomic DNA. Translation: ABF48499.1.
AK019491 mRNA. Translation: BAB31757.1.
AK028481 mRNA. Translation: BAC25974.1.
CH466585 Genomic DNA. Translation: EDL01690.1.
BC037116 mRNA. Translation: AAH37116.1.
CCDSiCCDS29900.1. [Q9QYB5-1]
CCDS50467.1. [Q9QYB5-2]
RefSeqiNP_001157571.1. NM_001164099.2. [Q9QYB5-1]
NP_001157572.1. NM_001164100.2. [Q9QYB5-2]
NP_001264029.1. NM_001277100.1. [Q9QYB5-1]
NP_038786.2. NM_013758.4. [Q9QYB5-1]
UniGeneiMm.426080.
Mm.491114.

Genome annotation databases

EnsembliENSMUST00000025999; ENSMUSP00000025999; ENSMUSG00000025026. [Q9QYB5-1]
ENSMUST00000050096; ENSMUSP00000052245; ENSMUSG00000025026. [Q9QYB5-2]
ENSMUST00000111741; ENSMUSP00000107370; ENSMUSG00000025026. [Q9QYB5-1]
GeneIDi27360.
KEGGimmu:27360.
UCSCiuc008hwi.3. mouse. [Q9QYB5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF100424 mRNA. Translation: AAF24974.1.
AF100425 mRNA. Translation: AAF24975.1.
DQ479918 Genomic DNA. Translation: ABF48499.1.
AK019491 mRNA. Translation: BAB31757.1.
AK028481 mRNA. Translation: BAC25974.1.
CH466585 Genomic DNA. Translation: EDL01690.1.
BC037116 mRNA. Translation: AAH37116.1.
CCDSiCCDS29900.1. [Q9QYB5-1]
CCDS50467.1. [Q9QYB5-2]
RefSeqiNP_001157571.1. NM_001164099.2. [Q9QYB5-1]
NP_001157572.1. NM_001164100.2. [Q9QYB5-2]
NP_001264029.1. NM_001277100.1. [Q9QYB5-1]
NP_038786.2. NM_013758.4. [Q9QYB5-1]
UniGeneiMm.426080.
Mm.491114.

3D structure databases

ProteinModelPortaliQ9QYB5.
SMRiQ9QYB5. Positions 138-373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205169. 1 interaction.
IntActiQ9QYB5. 2 interactions.
MINTiMINT-4087243.
STRINGi10090.ENSMUSP00000025999.

PTM databases

iPTMnetiQ9QYB5.
PhosphoSiteiQ9QYB5.

Proteomic databases

EPDiQ9QYB5.
MaxQBiQ9QYB5.
PaxDbiQ9QYB5.
PRIDEiQ9QYB5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025999; ENSMUSP00000025999; ENSMUSG00000025026. [Q9QYB5-1]
ENSMUST00000050096; ENSMUSP00000052245; ENSMUSG00000025026. [Q9QYB5-2]
ENSMUST00000111741; ENSMUSP00000107370; ENSMUSG00000025026. [Q9QYB5-1]
GeneIDi27360.
KEGGimmu:27360.
UCSCiuc008hwi.3. mouse. [Q9QYB5-1]

Organism-specific databases

CTDi120.
MGIiMGI:1351615. Add3.

Phylogenomic databases

eggNOGiKOG3699. Eukaryota.
COG0235. LUCA.
GeneTreeiENSGT00390000016462.
HOGENOMiHOG000116349.
HOVERGENiHBG004180.
InParanoidiQ9QYB5.
KOiK18622.
OMAiRTKITWM.
OrthoDBiEOG7HF1HR.
TreeFamiTF313003.

Enzyme and pathway databases

ReactomeiR-MMU-5223345. Miscellaneous transport and binding events.

Miscellaneous databases

ChiTaRSiAdd3. mouse.
PROiQ9QYB5.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QYB5.
CleanExiMM_ADD3.
ExpressionAtlasiQ9QYB5. baseline and differential.
GenevisibleiQ9QYB5. MM.

Family and domain databases

Gene3Di3.40.225.10. 1 hit.
InterProiIPR027772. ADD3.
IPR001303. Aldolase_II/adducin_N.
[Graphical view]
PANTHERiPTHR10672:SF5. PTHR10672:SF5. 1 hit.
PfamiPF00596. Aldolase_II. 1 hit.
[Graphical view]
SMARTiSM01007. Aldolase_II. 1 hit.
[Graphical view]
SUPFAMiSSF53639. SSF53639. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse adducin gene family: alternative splicing and chromosomal localization."
    Suriyapperuma S.P., Lozovatsky L., Ciciotte S.L., Peters L.L., Gilligan D.M.
    Mamm. Genome 11:16-23(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BTBR T+ tf/J.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Skin.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-677 AND SER-681, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31; SER-42; SER-64; SER-402; SER-414; SER-423; SER-583; SER-585; SER-590; SER-673; SER-677; SER-679; SER-681 AND SER-683, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiADDG_MOUSE
AccessioniPrimary (citable) accession number: Q9QYB5
Secondary accession number(s): Q8JZT6, Q9D2M5, Q9QYB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: June 28, 2011
Last modified: June 8, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.