Q9QYB1 (CLIC4_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 113.
History...
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Chloride intracellular channel protein 4 Short name=mc3s5/mtCLIC | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) [Reference proteome] | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus![]() |
Protein attributes
| Sequence length | 253 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3 By similarity. May play a role in angiogenesis. Ref.5 |
| Subunit structure | Monomer. Interacts with HRH30. Interacts with AKAP9 By similarity. |
| Subcellular location | Cytoplasm By similarity. Cytoplasmic vesicle membrane; Single-pass membrane protein By similarity. Nucleus By similarity. Cell membrane; Single-pass membrane protein By similarity. Mitochondrion. Note: Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles By similarity. Ref.1 Ref.4 |
| Tissue specificity | Detected in blood vessels in the retina (at protein level). Expressed to the greatest extent in vivo in heart, lung, liver, kidney, and skin. Ref.1 Ref.4 Ref.5 |
| Induction | Up-regulated by calcium ions in differentiating keratinocytes. Ref.4 |
| Domain | Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion By similarity. |
| Sequence similarities | Belongs to the chloride channel CLIC family. Contains 1 GST C-terminal domain. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| Pip5k1b | P70181 | 4 | EBI-645175,EBI-645167 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 253 | 252 | Chloride intracellular channel protein 4 | PRO_0000144211 | |||||
Regions | |||||||||
| Transmembrane | 37 – 57 | 21 | Helical; Note=After insertion into the membrane; Potential | ||||||
| Domain | 104 – 244 | 141 | GST C-terminal | ||||||
| Region | 2 – 101 | 100 | Required for insertion into the membrane By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine By similarity | ||||||
| Modified residue | 4 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 130 | 1 | N6-acetyllysine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 180 | 1 | D → Y in BAC27301. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "p53 and tumor necrosis factor alpha regulate the expression of a mitochondrial chloride channel protein." Fernandez-Salas E., Sagar M., Cheng C., Yuspa S.H., Weinberg W.C. J. Biol. Chem. 274:36488-36497(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Forelimb. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C3H/He. Tissue: Mammary gland and Osteoblast. |
| [4] | "CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation." Suh K.S., Mutoh M., Mutoh T., Li L., Ryscavage A., Crutchley J.M., Dumont R.A., Cheng C., Yuspa S.H. J. Cell Sci. 120:2631-2640(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION, SUBCELLULAR LOCATION. |
| [5] | "Chloride intracellular channel protein-4 functions in angiogenesis by supporting acidification of vacuoles along the intracellular tubulogenic pathway." Ulmasov B., Bruno J., Gordon N., Hartnett M.E., Edwards J.C. Am. J. Pathol. 174:1084-1096(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF102578 mRNA. Translation: AAF19055.1. AK031211 mRNA. Translation: BAC27301.1. BC046384 mRNA. Translation: AAH46384.1. BC052890 mRNA. Translation: AAH52890.1. |
| IPI | IPI00135977. |
| RefSeq | NP_038913.1. NM_013885.2. |
| UniGene | Mm.257765. |
3D structure databases | |
| ProteinModelPortal | Q9QYB1. |
| SMR | Q9QYB1. Positions 6-253. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9QYB1. 1 interaction. |
| MINT | MINT-1651993. |
PTM databases | |
| PhosphoSite | Q9QYB1. |
2D gel databases | |
| UCD-2DPAGE | Q9QYB1. |
Proteomic databases | |
| PaxDb | Q9QYB1. |
| PRIDE | Q9QYB1. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000037099; ENSMUSP00000041453; ENSMUSG00000037242. |
| GeneID | 29876. |
| KEGG | mmu:29876. |
| UCSC | uc008vgd.1. mouse. |
Organism-specific databases | |
| CTD | 25932. |
| MGI | MGI:1352754. Clic4. |
Phylogenomic databases | |
| eggNOG | NOG282171. |
| GeneTree | ENSGT00550000074477. |
| HOGENOM | HOG000231548. |
| HOVERGEN | HBG050994. |
| InParanoid | Q9QYB1. |
| KO | K05024. |
| OMA | IPKGMTG. |
| OrthoDB | EOG40S0FN. |
Gene expression databases | |
| ArrayExpress | Q9QYB1. |
| Bgee | Q9QYB1. |
| CleanEx | MM_CLIC4. |
| Genevestigator | Q9QYB1. |
| GermOnline | ENSMUSG00000037242. Mus musculus. |
Family and domain databases | |
| Gene3D | 1.20.1050.10. 1 hit. 3.40.30.10. 1 hit. |
| InterPro | IPR010987. Glutathione-S-Trfase_C-like. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR002946. Int_Cl_channel. IPR012336. Thioredoxin-like_fold. [Graphical view] |
| PRINTS | PR01263. INTCLCHANNEL. |
| SUPFAM | SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit. |
| TIGRFAMs | TIGR00862. O-ClC. 1 hit. |
| PROSITE | PS50405. GST_CTER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| ChiTaRS | CLIC4. mouse. |
| NextBio | 307114. |
| SOURCE | Search... |
Entry information
| Entry name | CLIC4_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q9QYB1 Secondary accession number(s): Q8BMG5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with
