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Reviewed, UniProtKB/Swiss-Prot Q9QYB1 (CLIC4_MOUSE)

Last modified December 15, 2009. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Chloride intracellular channel protein 4
      Short name=mc3s5/mtCLIC
Gene names
Name: Clic4
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length253 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3 By similarity. May play a role in angiogenesis.

Subunit structure

Monomer. Interacts with HRH3 By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasmic vesicle membrane; Single-pass membrane protein By similarity. Nucleus By similarity. Cell membrane; Single-pass membrane protein By similarity. Mitochondrion. Note: Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles By similarity.

Tissue specificity

Detected in blood vessels in the retina (at protein level). Expressed to the greatest extent in vivo in heart, lung, liver, kidney, and skin. Ref.1 Ref.4 Ref.5

Induction

Up-regulated by calcium ions in differentiating keratinocytes. Ref.4

Domain

Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion By similarity.

Sequence similarities

Belongs to the chloride channel CLIC family.

Contains 1 GST C-terminal domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Pip5k1bP701813EBI-645175,EBI-645167

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 253252Chloride intracellular channel protein 4
PRO_0000144211

Regions

Transmembrane37 – 5721After insertion into the membrane Potential
Domain81 – 244164GST C-terminal
Region2 – 101100Required for insertion into the membrane By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue1301N6-acetyllysine By similarity

Experimental info

Sequence conflict1801D → Y in BAC27301. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9QYB1-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 809568B37B441BB4

FASTA25328,729
        10         20         30         40         50         60 
MALSMPLNGL KEEDKEPLIE LFVKAGSDGE SIGNCPFSQR LFMILWLKGV VFSVTTVDLK 

        70         80         90        100        110        120 
RKPADLQNLA PGTHPPFITF NSEVKTDVNK IEEFLEEVLC PPKYLKLSPK HPESNTAGMD 

       130        140        150        160        170        180 
IFAKFSAYIK NSRPEANEAL ERGLLKTLQK LDEYLNSPLP DEIDENSMED IKFSTRRFLD 

       190        200        210        220        230        240 
GDEMTLADCN LLPKLHIVKV VAKKYRNFDI PKGMTGIWRY LTNAYSRDEF TNTCPSDKEV 

       250 
EIAYSDVAKR LTK 

« Hide

References

« Hide 'large scale' references
[1]"p53 and tumor necrosis factor alpha regulate the expression of a mitochondrial chloride channel protein."
Fernandez-Salas E., Sagar M., Cheng C., Yuspa S.H., Weinberg W.C.
J. Biol. Chem. 274:36488-36497(1999) [PubMed: 10593946] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Forelimb.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C3H/He.
Tissue: Mammary gland and Osteoblast.
[4]"CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation."
Suh K.S., Mutoh M., Mutoh T., Li L., Ryscavage A., Crutchley J.M., Dumont R.A., Cheng C., Yuspa S.H.
J. Cell Sci. 120:2631-2640(2007) [PubMed: 17636002] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION, SUBCELLULAR LOCATION.
[5]"Chloride intracellular channel protein-4 functions in angiogenesis by supporting acidification of vacuoles along the intracellular tubulogenic pathway."
Ulmasov B., Bruno J., Gordon N., Hartnett M.E., Edwards J.C.
Am. J. Pathol. 174:1084-1096(2009) [PubMed: 19197003] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

AF102578 mRNA. Translation: AAF19055.1.
AK031211 mRNA. Translation: BAC27301.1.
BC046384 mRNA. Translation: AAH46384.1.
BC052890 mRNA. Translation: AAH52890.1.
IPIIPI00135977.
RefSeqNP_038913.1.
UniGeneMm.257765
Mm.473864

3D structure databases

SMRQ9QYB1. Positions 5-252, 6-253.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9QYB1. 1 interaction.
STRINGQ9QYB1.

PTM databases

PhosphoSiteQ9QYB1.

Proteomic databases

PRIDEQ9QYB1.

Genome annotation databases

EnsemblENSMUST00000037099; ENSMUSP00000041453; ENSMUSG00000037242; Mus musculus. [Genome view]
GeneID29876.
KEGGmmu:29876.
NMPDRfig|10090.3.peg.10581.
UCSCuc008vgd.1. mouse.

Organism-specific databases

CTD29876.
MGIMGI:1352754. Clic4.

Phylogenomic databases

HOGENOMHBG444630.
HOVERGENQ9QYB1.
InParanoidQ9QYB1.
OMAPFITFNG.
OrthoDBEOG9QNQFW.

Gene expression databases

ArrayExpressQ9QYB1.
BgeeQ9QYB1.
CleanExMM_CLIC4.
GenevestigatorQ9QYB1.
GermOnlineENSMUSG00000037242. Mus musculus.

Family and domain databases

InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR017933. Glutathione_S_Trfase/Cl_chnl_C.
IPR002946. Int_Cl_channel.
IPR012336. Thioredoxin-like_fold.
IPR012335. Thioredoxin_fold.
[Graphical view]
Gene3DG3DSA:1.20.1050.10. GST_C_like. 1 hit.
G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PRINTSPR01263. INTCLCHANNEL.
PROSITEPS50405. GST_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio307114.
SOURCESearch...

Entry information

Entry nameCLIC4_MOUSE
AccessionPrimary (citable) accession number: Q9QYB1
Secondary accession number(s): Q8BMG5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: January 23, 2007
Last modified: December 15, 2009
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents