Q9QYB1 (CLIC4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chloride intracellular channel protein 4
Short name=mc3s5/mtCLIC

Gene names

Name:

Clic4

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3 By similarity. May play a role in angiogenesis. Ref.5
Subunit structure	Monomer. Interacts with HRH30. Interacts with AKAP9 By similarity.
Subcellular location	Cytoplasm By similarity. Cytoplasmic vesicle membrane; Single-pass membrane protein By similarity. Nucleus By similarity. Cell membrane; Single-pass membrane protein By similarity. Mitochondrion. Note: Exists both as soluble cytoplasmic protein and as membrane protein with probably a single transmembrane domain. Present in an intracellular vesicular compartment that likely represent trans-Golgi network vesicles By similarity. Ref.1 Ref.4
Tissue specificity	Detected in blood vessels in the retina (at protein level). Expressed to the greatest extent in vivo in heart, lung, liver, kidney, and skin. Ref.1 Ref.4 Ref.5
Induction	Up-regulated by calcium ions in differentiating keratinocytes. Ref.4
Domain	Members of this family may change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion By similarity.
Sequence similarities	Belongs to the chloride channel CLIC family. Contains 1 GST C-terminal domain.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell membrane Cytoplasm Cytoplasmic vesicle Membrane Mitochondrion Nucleus
Domain	Transmembrane Transmembrane helix
Ligand	Chloride
Molecular function	Chloride channel Ionic channel Voltage-gated channel
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	keratinocyte differentiation Inferred from mutant phenotype Ref.4. Source: UniProtKB negative regulation of cell migration Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	chloride channel complex Inferred from electronic annotation. Source: UniProtKB-KW cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microvillus Inferred from sequence or structural similarity. Source: UniProtKB mitochondrion Inferred from direct assay Ref.1. Source: MGI plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: IntAct voltage-gated chloride channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pip5k1b	P70181	4	EBI-645175,EBI-645167

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 253	252	Chloride intracellular channel protein 4		PRO_0000144211
Regions
	Transmembrane	37 – 57	21	Helical; Note=After insertion into the membrane; Potential
	Domain	104 – 244	141	GST C-terminal
	Region	2 – 101	100	Required for insertion into the membrane By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	130	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	180	1	D → Y in BAC27301. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9QYB1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 809568B37B441BB4	FASTA	253	28,729
	10 20 30 40 50 60 MALSMPLNGL KEEDKEPLIE LFVKAGSDGE SIGNCPFSQR LFMILWLKGV VFSVTTVDLK 70 80 90 100 110 120 RKPADLQNLA PGTHPPFITF NSEVKTDVNK IEEFLEEVLC PPKYLKLSPK HPESNTAGMD 130 140 150 160 170 180 IFAKFSAYIK NSRPEANEAL ERGLLKTLQK LDEYLNSPLP DEIDENSMED IKFSTRRFLD 190 200 210 220 230 240 GDEMTLADCN LLPKLHIVKV VAKKYRNFDI PKGMTGIWRY LTNAYSRDEF TNTCPSDKEV 250 EIAYSDVAKR LTK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"p53 and tumor necrosis factor alpha regulate the expression of a mitochondrial chloride channel protein." Fernandez-Salas E., Sagar M., Cheng C., Yuspa S.H., Weinberg W.C. J. Biol. Chem. 274:36488-36497(1999) [PubMed: 10593946] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Forelimb.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C3H/He. Tissue: Mammary gland and Osteoblast.
[4]	"CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation." Suh K.S., Mutoh M., Mutoh T., Li L., Ryscavage A., Crutchley J.M., Dumont R.A., Cheng C., Yuspa S.H. J. Cell Sci. 120:2631-2640(2007) [PubMed: 17636002] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION, SUBCELLULAR LOCATION.
[5]	"Chloride intracellular channel protein-4 functions in angiogenesis by supporting acidification of vacuoles along the intracellular tubulogenic pathway." Ulmasov B., Bruno J., Gordon N., Hartnett M.E., Edwards J.C. Am. J. Pathol. 174:1084-1096(2009) [PubMed: 19197003] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF102578 mRNA. Translation: AAF19055.1. AK031211 mRNA. Translation: BAC27301.1. BC046384 mRNA. Translation: AAH46384.1. BC052890 mRNA. Translation: AAH52890.1.
IPI	IPI00135977.
RefSeq	NP_038913.1. NM_013885.2.
UniGene	Mm.257765.
3D structure databases
ProteinModelPortal	Q9QYB1.
SMR	Q9QYB1. Positions 6-253.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9QYB1. 1 interaction.
STRING	Q9QYB1.
PTM databases
PhosphoSite	Q9QYB1.
2D gel databases
UCD-2DPAGE	Q9QYB1.
Proteomic databases
PRIDE	Q9QYB1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000037099; ENSMUSP00000041453; ENSMUSG00000037242.
GeneID	29876.
KEGG	mmu:29876.
NMPDR	fig|10090.3.peg.10581.
Organism-specific databases
CTD	25932.
MGI	MGI:1352754. Clic4.
Phylogenomic databases
GeneTree	ENSGT00550000074477.
HOGENOM	HBG444630.
HOVERGEN	HBG050994.
InParanoid	Q9QYB1.
OMA	FLEDVLC.
OrthoDB	EOG40S0FN.
PhylomeDB	Q9QYB1.
Gene expression databases
ArrayExpress	Q9QYB1.
Bgee	Q9QYB1.
CleanEx	MM_CLIC4.
Genevestigator	Q9QYB1.
GermOnline	ENSMUSG00000037242. Mus musculus.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR002946. Int_Cl_channel. IPR012336. Thioredoxin-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KO	K05024.
PRINTS	PR01263. INTCLCHANNEL.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
TIGRFAMs	TIGR00862. O-ClC. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	307114.
SOURCE	Search...

Entry information

Entry name

CLIC4_MOUSE

Accession

Primary (citable) accession number: Q9QYB1
Secondary accession number(s): Q8BMG5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	January 23, 2007
Last modified:	November 16, 2011
This is version 101 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program