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Q9QY96 (CASR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Extracellular calcium-sensing receptor

Short name=CaSR
Alternative name(s):
Parathyroid cell calcium-sensing receptor
Short name=PCaR1
Gene names
Name:Casr
Synonyms:Gprc2a
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1079 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.

Subunit structure

Interacts with VCP and RNF19A. Interacts with ARRB1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Epidermis, kidney and cartilage. Ref.1 Ref.2 Ref.5 Ref.8

Post-translational modification

N-glycosylated By similarity.

Ubiquitinated by RNF19A; which induces proteasomal degradation By similarity.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMGlycoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processJNK cascade

Inferred from electronic annotation. Source: Ensembl

adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

apoptotic process

Inferred from electronic annotation. Source: Ensembl

branching morphogenesis of an epithelial tube

Inferred from electronic annotation. Source: Ensembl

calcium ion transport

Inferred from electronic annotation. Source: Ensembl

cellular calcium ion homeostasis

Inferred from electronic annotation. Source: Ensembl

cellular response to glucose stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to hepatocyte growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to hypoxia

Inferred from electronic annotation. Source: Ensembl

cellular response to low-density lipoprotein particle stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to peptide

Inferred from electronic annotation. Source: Ensembl

cellular response to vitamin D

Inferred from electronic annotation. Source: Ensembl

fat pad development

Inferred from electronic annotation. Source: Ensembl

ossification

Inferred from electronic annotation. Source: Ensembl

phospholipase C-activating G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive regulation of ATPase activity

Inferred from direct assay PubMed 17823248. Source: MGI

positive regulation of calcium ion import

Inferred from electronic annotation. Source: Ensembl

positive regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of gene expression

Inferred from direct assay PubMed 18794335. Source: MGI

positive regulation of insulin secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of positive chemotaxis

Inferred from electronic annotation. Source: Ensembl

positive regulation of vasoconstriction

Inferred from electronic annotation. Source: Ensembl

regulation of calcium ion transport

Inferred from direct assay PubMed 17823248. Source: MGI

response to calcium ion

Inferred from electronic annotation. Source: Ensembl

response to fibroblast growth factor

Inferred from electronic annotation. Source: Ensembl

response to ischemia

Inferred from electronic annotation. Source: Ensembl

vasodilation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentapical plasma membrane

Inferred from electronic annotation. Source: Ensembl

axon terminus

Inferred from electronic annotation. Source: Ensembl

basolateral plasma membrane

Inferred from electronic annotation. Source: Ensembl

cell surface

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

calcium ion binding

Inferred from electronic annotation. Source: Ensembl

drug binding

Inferred from electronic annotation. Source: Ensembl

magnesium ion binding

Inferred from electronic annotation. Source: Ensembl

polyamine binding

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: Q9QY96-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: Q9QY96-2)

The sequence of this isoform differs from the canonical sequence as follows:
     461-537: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Potential
Chain20 – 10791060Extracellular calcium-sensing receptor
PRO_0000012947

Regions

Topological domain20 – 612593Extracellular Potential
Transmembrane613 – 63523Helical; Name=1; Potential
Topological domain636 – 64914Cytoplasmic Potential
Transmembrane650 – 67021Helical; Name=2; Potential
Topological domain671 – 68111Extracellular Potential
Transmembrane682 – 70019Helical; Name=3; Potential
Topological domain701 – 72424Cytoplasmic Potential
Transmembrane725 – 74521Helical; Name=4; Potential
Topological domain746 – 76924Extracellular Potential
Transmembrane770 – 79223Helical; Name=5; Potential
Topological domain793 – 80513Cytoplasmic Potential
Transmembrane806 – 82823Helical; Name=6; Potential
Topological domain829 – 8368Extracellular Potential
Transmembrane837 – 86226Helical; Name=7; Potential
Topological domain863 – 1079217Cytoplasmic Potential
Region880 – 90021Interaction with RNF19A By similarity

Amino acid modifications

Glycosylation901N-linked (GlcNAc...) Potential
Glycosylation1301N-linked (GlcNAc...) Potential
Glycosylation2611N-linked (GlcNAc...) Potential
Glycosylation2871N-linked (GlcNAc...) Potential
Glycosylation3861N-linked (GlcNAc...) Potential
Glycosylation4461N-linked (GlcNAc...) Potential
Glycosylation4681N-linked (GlcNAc...) Potential
Glycosylation4881N-linked (GlcNAc...) Potential
Glycosylation5411N-linked (GlcNAc...) Potential
Glycosylation5941N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence461 – 53777Missing in isoform B.
VSP_002036

Experimental info

Sequence conflict451A → S in AAD40638. Ref.2
Sequence conflict3041L → P in AAF00193. Ref.5
Sequence conflict4101D → G in AAD28371. Ref.1
Sequence conflict4101D → G in AAD28372. Ref.1
Sequence conflict5661V → A in AAD40638. Ref.2
Sequence conflict5951H → Y in AAD28371. Ref.1
Sequence conflict5951H → Y in AAD28372. Ref.1
Sequence conflict6101E → V in BAA77688. Ref.7
Sequence conflict8141F → L in BAA77688. Ref.7
Sequence conflict8891L → I in AAD40638. Ref.2
Sequence conflict906 – 9083TGS → SGW in AAD40638. Ref.2
Sequence conflict9091I → N in AAD28371. Ref.1
Sequence conflict9091I → N in AAD28372. Ref.1
Sequence conflict10571M → V in AAD28371. Ref.1
Sequence conflict10571M → V in AAD28372. Ref.1
Sequence conflict10641V → A in AAD40638. Ref.2
Sequence conflict10761I → V in AAD40638. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified October 3, 2012. Version 2.
Checksum: 8AEF586CDCD93CB3

FASTA1,079120,903
        10         20         30         40         50         60 
MAWFGYCLAL LALTWHSSAY GPDQRAQKKG DIILGGLFPI HFGVAAKDQD LKSRPESVEC 

        70         80         90        100        110        120 
IRYNFRGFRW LQAMIFAIEE INSSPALLPN MTLGYRIFDT CNTVSKALEA TLSFVAQNKI 

       130        140        150        160        170        180 
DSLNLDEFCN CSEHIPSTIA VVGATGSGVS TAVANLLGLF YIPQVSYASS SRLLSNKNQF 

       190        200        210        220        230        240 
KSFLRTIPND EHQATAMADI IEYFRWNWVG TIAADDDYGR PGIEKFREEA EERDICIDFS 

       250        260        270        280        290        300 
ELISQYSDEE EIQQVVEVIQ NSTAKVIVVF SSGPDLEPLI KEIVRRNITG RIWLASEAWA 

       310        320        330        340        350        360 
SSSLIAMPEY FHVVGGTIGF GLKAGQIPGF REFLQKVHPR KSVHNGFAKE FWEETFNCHL 

       370        380        390        400        410        420 
QDGAKGPLPV DTFVRSHEEG GNRLLNSSTA FRPLCTGDEN INSVETPYMD YEHLRISYNV 

       430        440        450        460        470        480 
YLAVYSIAHA LQDIYTCLPG RGLFTNGSCA DIKKVEAWQV LKHLRHLNFT NNMGEQVTFD 

       490        500        510        520        530        540 
ECGDLVGNYS IINWHLSPED GSIVFKEVGY YNVYAKKGER LFINEGKILW SGFSREVPFS 

       550        560        570        580        590        600 
NCSRDCQAGT RKGIIEGEPT CCFECVECPD GEYSGETDAS ACDKCPDDFW SNENHTSCIA 

       610        620        630        640        650        660 
KEIEFLAWTE PFGIALTLFA VLGIFLTAFV LGVFIKFRNT PIVKATNREL SYLLLFSLLC 

       670        680        690        700        710        720 
CFSSSLFFIG EPQDWTCRLR QPAFGISFVL CISCILVKTN RVLLVFEAKI PTSFHRKWWG 

       730        740        750        760        770        780 
LNLQFLLVFL CTFMQIVICI IWLYTAPPSS YRNHELEDEI IFITCHEGSL MALGSLIGYT 

       790        800        810        820        830        840 
CLLAAICFFF AFKSRKLPEN FNEAKFITFS MLIFFIVWIS FIPAYASTYG KFVSAVEVIA 

       850        860        870        880        890        900 
ILAASFGLLA CIFFNKVYII LFKPSRNTIE EVRSSTAAHA FKVAARATLR RPNISRKRSS 

       910        920        930        940        950        960 
SLGGSTGSIP SSSISSKSNS EDRFPQPERQ KQQQPLALTQ QEQQQQPLTL QPQQQQQPQQ 

       970        980        990       1000       1010       1020 
PRCKQKVIFG SGTVTFSLSF DEPQKNAMAH RNSMRQNSLE AQKSNDTLNR HQALLPLQCA 

      1030       1040       1050       1060       1070 
EADSEMTIQE TGLQGPMVGD HQPEIESPDE MSPALVMSTS RSFVISGGGS SVTENILHS 

« Hide

Isoform B [UniParc].

Checksum: 94BF8B687C0FD877
Show »

FASTA1,002111,996

References

« Hide 'large scale' references
[1]"The calcium sensing receptor and its alternatively spliced form in murine epidermal differentiation."
Oda Y., Tu C.-L., Chang W., Crumrine D., Koemueves L., Mauro T., Elias P.M., Bikle D.D.
J. Biol. Chem. 275:1183-1190(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY.
Strain: C57BL/6.
Tissue: Kidney.
[2]"Sensing of extracellular cations in CasR-deficient osteoblasts. Evidence for a novel cation-sensing mechanism."
Pi M., Garner S.C., Flannery P., Spurney R.F., Quarles L.D.
J. Biol. Chem. 275:3256-3263(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY.
Strain: Black Swiss X 129/SVJ.
Tissue: Kidney.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Expression and signal transduction of calcium-sensing receptors in cartilage and bone."
Chang W., Tu C., Chen T.-H., Komuves L., Oda Y., Pratt S.A., Miller S., Shoback D.
Endocrinology 140:5883-5893(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 256-600 (ISOFORM A), TISSUE SPECIFICITY.
Tissue: Epiphyseal cartilage.
[6]Hildenbrand J., Ammon H.P.T., Wahl M.A.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 507-582 (ISOFORM A).
Strain: NMRI.
Tissue: Brain.
[7]Moawad T.I., Riccardi D.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 562-814.
Tissue: Kidney.
[8]"A distinct cation-sensing mechanism in MC3T3-E1 osteoblasts functionally related to the calcium receptor."
Quarles L.D., Hartle J.E. II, Siddhanti S.R., Guo R., Hinson T.K.
J. Bone Miner. Res. 12:393-402(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 646-799, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF110178 mRNA. Translation: AAD28371.1.
AF110179 mRNA. Translation: AAD28372.1.
AF128842 mRNA. Translation: AAD40638.1.
AC074229 Genomic DNA. No translation available.
CH466521 Genomic DNA. Translation: EDK97924.1.
AF068900 mRNA. Translation: AAC19388.1.
AB027140 mRNA. Translation: BAA77688.1.
AF002015 Genomic DNA. Translation: AAC53252.1.
AF159565 mRNA. Translation: AAF00193.1.
CCDSCCDS28154.1. [Q9QY96-1]
RefSeqNP_038831.2. NM_013803.2. [Q9QY96-1]
XP_006521792.1. XM_006521729.1. [Q9QY96-1]
XP_006521793.1. XM_006521730.1. [Q9QY96-1]
XP_006521794.1. XM_006521731.1. [Q9QY96-1]
XP_006521795.1. XM_006521732.1. [Q9QY96-1]
XP_006521796.1. XM_006521733.1. [Q9QY96-1]
XP_006521797.1. XM_006521734.1. [Q9QY96-1]
UniGeneMm.103619.

3D structure databases

ProteinModelPortalQ9QY96.
SMRQ9QY96. Positions 29-531, 603-864.
ModBaseSearch...
MobiDBSearch...

Chemistry

GuidetoPHARMACOLOGY54.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteQ9QY96.

Proteomic databases

PaxDbQ9QY96.
PRIDEQ9QY96.

Protocols and materials databases

DNASU12374.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000063597; ENSMUSP00000069080; ENSMUSG00000051980. [Q9QY96-1]
ENSMUST00000114847; ENSMUSP00000110496; ENSMUSG00000051980. [Q9QY96-2]
ENSMUST00000172826; ENSMUSP00000133500; ENSMUSG00000051980. [Q9QY96-1]
GeneID12374.
KEGGmmu:12374.
UCSCuc007zcp.1. mouse.

Organism-specific databases

CTD846.
MGIMGI:1351351. Casr.

Phylogenomic databases

eggNOGNOG295200.
GeneTreeENSGT00750000117616.
HOVERGENHBG052876.
InParanoidQ9QY96.
KOK04612.
OMAIILFKPS.
OrthoDBEOG7DZ8J8.
TreeFamTF331269.

Gene expression databases

ArrayExpressQ9QY96.
CleanExMM_CASR.
GenevestigatorQ9QY96.

Family and domain databases

InterProIPR001828. ANF_lig-bd_rcpt.
IPR000337. GPCR_3.
IPR011500. GPCR_3_9-Cys_dom.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
SUPFAMSSF53822. SSF53822. 1 hit.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio281082.
PROQ9QY96.
SOURCESearch...

Entry information

Entry nameCASR_MOUSE
AccessionPrimary (citable) accession number: Q9QY96
Secondary accession number(s): G3UX06 expand/collapse secondary AC list , O08968, O88519, Q9QY95, Q9QZU8, Q9R1D6, Q9R1Y2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 3, 2012
Last modified: July 9, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries