Q9QY96 (CASR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Extracellular calcium-sensing receptor
Short name=CaSR

Alternative name(s):
Parathyroid cell calcium-sensing receptor
Short name=PCaR1

Gene names

Name:	Casr
Synonyms:	Gprc2a

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1079 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.
Subunit structure	Interacts with VCP and RNF19A. Interacts with ARRB1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Epidermis, kidney and cartilage. Ref.1 Ref.2 Ref.5 Ref.8
Post-translational modification	N-glycosylated By similarity. Ubiquitinated by RNF19A; which induces proteasomal degradation By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 3 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Glycoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	JNK cascade Inferred from electronic annotation. Source: Compara calcium ion transport Inferred from electronic annotation. Source: Compara cellular calcium ion homeostasis Inferred from electronic annotation. Source: Compara ossification Inferred from electronic annotation. Source: Compara positive regulation of ATPase activity Inferred from direct assay PubMed 17823248. Source: MGI positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of gene expression Inferred from direct assay PubMed 18794335. Source: MGI regulation of calcium ion transport Inferred from direct assay PubMed 17823248. Source: MGI vasodilation Inferred from electronic annotation. Source: Compara
Cellular_component	axon terminus Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: Q9QY96-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: Q9QY96-2)

The sequence of this isoform differs from the canonical sequence as follows:
     461-537: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 1079	1060	Extracellular calcium-sensing receptor		PRO_0000012947
Regions
	Topological domain	20 – 612	593	Extracellular Potential
	Transmembrane	613 – 635	23	Helical; Name=1; Potential
	Topological domain	636 – 649	14	Cytoplasmic Potential
	Transmembrane	650 – 670	21	Helical; Name=2; Potential
	Topological domain	671 – 681	11	Extracellular Potential
	Transmembrane	682 – 700	19	Helical; Name=3; Potential
	Topological domain	701 – 724	24	Cytoplasmic Potential
	Transmembrane	725 – 745	21	Helical; Name=4; Potential
	Topological domain	746 – 769	24	Extracellular Potential
	Transmembrane	770 – 792	23	Helical; Name=5; Potential
	Topological domain	793 – 805	13	Cytoplasmic Potential
	Transmembrane	806 – 828	23	Helical; Name=6; Potential
	Topological domain	829 – 836	8	Extracellular Potential
	Transmembrane	837 – 862	26	Helical; Name=7; Potential
	Topological domain	863 – 1079	217	Cytoplasmic Potential
	Region	880 – 900	21	Interaction with RNF19A By similarity
Amino acid modifications
	Glycosylation	90	1	N-linked (GlcNAc...) Potential
	Glycosylation	130	1	N-linked (GlcNAc...) Potential
	Glycosylation	261	1	N-linked (GlcNAc...) Potential
	Glycosylation	287	1	N-linked (GlcNAc...) Potential
	Glycosylation	386	1	N-linked (GlcNAc...) Potential
	Glycosylation	446	1	N-linked (GlcNAc...) Potential
	Glycosylation	468	1	N-linked (GlcNAc...) Potential
	Glycosylation	488	1	N-linked (GlcNAc...) Potential
	Glycosylation	541	1	N-linked (GlcNAc...) Potential
	Glycosylation	594	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	461 – 537	77	Missing in isoform B.		VSP_002036
Experimental info
	Sequence conflict	45	1	A → S in AAD40638. Ref.2
	Sequence conflict	304	1	L → P in AAF00193. Ref.5
	Sequence conflict	410	1	D → G in AAD28371. Ref.1
	Sequence conflict	410	1	D → G in AAD28372. Ref.1
	Sequence conflict	566	1	V → A in AAD40638. Ref.2
	Sequence conflict	595	1	H → Y in AAD28371. Ref.1
	Sequence conflict	595	1	H → Y in AAD28372. Ref.1
	Sequence conflict	610	1	E → V in BAA77688. Ref.7
	Sequence conflict	814	1	F → L in BAA77688. Ref.7
	Sequence conflict	889	1	L → I in AAD40638. Ref.2
	Sequence conflict	906 – 908	3	TGS → SGW in AAD40638. Ref.2
	Sequence conflict	909	1	I → N in AAD28371. Ref.1
	Sequence conflict	909	1	I → N in AAD28372. Ref.1
	Sequence conflict	1057	1	M → V in AAD28371. Ref.1
	Sequence conflict	1057	1	M → V in AAD28372. Ref.1
	Sequence conflict	1064	1	V → A in AAD40638. Ref.2
	Sequence conflict	1076	1	I → V in AAD40638. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified October 3, 2012. Version 2. Checksum: 8AEF586CDCD93CB3	FASTA	1,079	120,903
	10 20 30 40 50 60 MAWFGYCLAL LALTWHSSAY GPDQRAQKKG DIILGGLFPI HFGVAAKDQD LKSRPESVEC 70 80 90 100 110 120 IRYNFRGFRW LQAMIFAIEE INSSPALLPN MTLGYRIFDT CNTVSKALEA TLSFVAQNKI 130 140 150 160 170 180 DSLNLDEFCN CSEHIPSTIA VVGATGSGVS TAVANLLGLF YIPQVSYASS SRLLSNKNQF 190 200 210 220 230 240 KSFLRTIPND EHQATAMADI IEYFRWNWVG TIAADDDYGR PGIEKFREEA EERDICIDFS 250 260 270 280 290 300 ELISQYSDEE EIQQVVEVIQ NSTAKVIVVF SSGPDLEPLI KEIVRRNITG RIWLASEAWA 310 320 330 340 350 360 SSSLIAMPEY FHVVGGTIGF GLKAGQIPGF REFLQKVHPR KSVHNGFAKE FWEETFNCHL 370 380 390 400 410 420 QDGAKGPLPV DTFVRSHEEG GNRLLNSSTA FRPLCTGDEN INSVETPYMD YEHLRISYNV 430 440 450 460 470 480 YLAVYSIAHA LQDIYTCLPG RGLFTNGSCA DIKKVEAWQV LKHLRHLNFT NNMGEQVTFD 490 500 510 520 530 540 ECGDLVGNYS IINWHLSPED GSIVFKEVGY YNVYAKKGER LFINEGKILW SGFSREVPFS 550 560 570 580 590 600 NCSRDCQAGT RKGIIEGEPT CCFECVECPD GEYSGETDAS ACDKCPDDFW SNENHTSCIA 610 620 630 640 650 660 KEIEFLAWTE PFGIALTLFA VLGIFLTAFV LGVFIKFRNT PIVKATNREL SYLLLFSLLC 670 680 690 700 710 720 CFSSSLFFIG EPQDWTCRLR QPAFGISFVL CISCILVKTN RVLLVFEAKI PTSFHRKWWG 730 740 750 760 770 780 LNLQFLLVFL CTFMQIVICI IWLYTAPPSS YRNHELEDEI IFITCHEGSL MALGSLIGYT 790 800 810 820 830 840 CLLAAICFFF AFKSRKLPEN FNEAKFITFS MLIFFIVWIS FIPAYASTYG KFVSAVEVIA 850 860 870 880 890 900 ILAASFGLLA CIFFNKVYII LFKPSRNTIE EVRSSTAAHA FKVAARATLR RPNISRKRSS 910 920 930 940 950 960 SLGGSTGSIP SSSISSKSNS EDRFPQPERQ KQQQPLALTQ QEQQQQPLTL QPQQQQQPQQ 970 980 990 1000 1010 1020 PRCKQKVIFG SGTVTFSLSF DEPQKNAMAH RNSMRQNSLE AQKSNDTLNR HQALLPLQCA 1030 1040 1050 1060 1070 EADSEMTIQE TGLQGPMVGD HQPEIESPDE MSPALVMSTS RSFVISGGGS SVTENILHS
	Isoform B [UniParc]. Checksum: 94BF8B687C0FD877	FASTA	1,002	111,996
	10 20 30 40 50 60 MAWFGYCLAL LALTWHSSAY GPDQRAQKKG DIILGGLFPI HFGVAAKDQD LKSRPESVEC 70 80 90 100 110 120 IRYNFRGFRW LQAMIFAIEE INSSPALLPN MTLGYRIFDT CNTVSKALEA TLSFVAQNKI 130 140 150 160 170 180 DSLNLDEFCN CSEHIPSTIA VVGATGSGVS TAVANLLGLF YIPQVSYASS SRLLSNKNQF 190 200 210 220 230 240 KSFLRTIPND EHQATAMADI IEYFRWNWVG TIAADDDYGR PGIEKFREEA EERDICIDFS 250 260 270 280 290 300 ELISQYSDEE EIQQVVEVIQ NSTAKVIVVF SSGPDLEPLI KEIVRRNITG RIWLASEAWA 310 320 330 340 350 360 SSSLIAMPEY FHVVGGTIGF GLKAGQIPGF REFLQKVHPR KSVHNGFAKE FWEETFNCHL 370 380 390 400 410 420 QDGAKGPLPV DTFVRSHEEG GNRLLNSSTA FRPLCTGDEN INSVETPYMD YEHLRISYNV 430 440 450 460 470 480 YLAVYSIAHA LQDIYTCLPG RGLFTNGSCA DIKKVEAWQV PFSNCSRDCQ AGTRKGIIEG 490 500 510 520 530 540 EPTCCFECVE CPDGEYSGET DASACDKCPD DFWSNENHTS CIAKEIEFLA WTEPFGIALT 550 560 570 580 590 600 LFAVLGIFLT AFVLGVFIKF RNTPIVKATN RELSYLLLFS LLCCFSSSLF FIGEPQDWTC 610 620 630 640 650 660 RLRQPAFGIS FVLCISCILV KTNRVLLVFE AKIPTSFHRK WWGLNLQFLL VFLCTFMQIV 670 680 690 700 710 720 ICIIWLYTAP PSSYRNHELE DEIIFITCHE GSLMALGSLI GYTCLLAAIC FFFAFKSRKL 730 740 750 760 770 780 PENFNEAKFI TFSMLIFFIV WISFIPAYAS TYGKFVSAVE VIAILAASFG LLACIFFNKV 790 800 810 820 830 840 YIILFKPSRN TIEEVRSSTA AHAFKVAARA TLRRPNISRK RSSSLGGSTG SIPSSSISSK 850 860 870 880 890 900 SNSEDRFPQP ERQKQQQPLA LTQQEQQQQP LTLQPQQQQQ PQQPRCKQKV IFGSGTVTFS 910 920 930 940 950 960 LSFDEPQKNA MAHRNSMRQN SLEAQKSNDT LNRHQALLPL QCAEADSEMT IQETGLQGPM 970 980 990 1000 VGDHQPEIES PDEMSPALVM STSRSFVISG GGSSVTENIL HS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The calcium sensing receptor and its alternatively spliced form in murine epidermal differentiation." Oda Y., Tu C.-L., Chang W., Crumrine D., Koemueves L., Mauro T., Elias P.M., Bikle D.D. J. Biol. Chem. 275:1183-1190(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Kidney.
[2]	"Sensing of extracellular cations in CasR-deficient osteoblasts. Evidence for a novel cation-sensing mechanism." Pi M., Garner S.C., Flannery P., Spurney R.F., Quarles L.D. J. Biol. Chem. 275:3256-3263(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY. Strain: Black Swiss X 129/SVJ. Tissue: Kidney.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"Expression and signal transduction of calcium-sensing receptors in cartilage and bone." Chang W., Tu C., Chen T.-H., Komuves L., Oda Y., Pratt S.A., Miller S., Shoback D. Endocrinology 140:5883-5893(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 256-600 (ISOFORM A), TISSUE SPECIFICITY. Tissue: Epiphyseal cartilage.
[6]	Hildenbrand J., Ammon H.P.T., Wahl M.A. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 507-582 (ISOFORM A). Strain: NMRI. Tissue: Brain.
[7]	Moawad T.I., Riccardi D. Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 562-814. Tissue: Kidney.
[8]	"A distinct cation-sensing mechanism in MC3T3-E1 osteoblasts functionally related to the calcium receptor." Quarles L.D., Hartle J.E. II, Siddhanti S.R., Guo R., Hinson T.K. J. Bone Miner. Res. 12:393-402(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 646-799, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF110178 mRNA. Translation: AAD28371.1. AF110179 mRNA. Translation: AAD28372.1. AF128842 mRNA. Translation: AAD40638.1. AC074229 Genomic DNA. No translation available. CH466521 Genomic DNA. Translation: EDK97924.1. AF068900 mRNA. Translation: AAC19388.1. AB027140 mRNA. Translation: BAA77688.1. AF002015 Genomic DNA. Translation: AAC53252.1. AF159565 mRNA. Translation: AAF00193.1.
IPI	IPI00135702. IPI00230189.
RefSeq	NP_038831.2. NM_013803.2.
UniGene	Mm.103619.
3D structure databases
ProteinModelPortal	Q9QY96.
SMR	Q9QY96. Positions 29-531.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	Q9QY96.
Proteomic databases
PaxDb	Q9QY96.
PRIDE	Q9QY96.
Protocols and materials databases
DNASU	12374.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000063597; ENSMUSP00000069080; ENSMUSG00000051980. ENSMUST00000114847; ENSMUSP00000110496; ENSMUSG00000051980. ENSMUST00000172826; ENSMUSP00000133500; ENSMUSG00000051980.
GeneID	12374.
KEGG	mmu:12374.
UCSC	uc007zcp.1. mouse. uc012afg.1. mouse.
Organism-specific databases
CTD	846.
MGI	MGI:1351351. Casr.
Phylogenomic databases
eggNOG	NOG295200.
GeneTree	ENSGT00690000101821.
HOVERGEN	HBG052876.
InParanoid	Q9QY96.
KO	K04612.
OMA	EPQKNAM.
OrthoDB	EOG4CJVGD.
Gene expression databases
CleanEx	MM_CASR.
Genevestigator	Q9QY96.
GermOnline	ENSMUSG00000051980. Mus musculus.
Family and domain databases
InterPro	IPR001828. ANF_lig-bd_rcpt. IPR000337. GPCR_3. IPR011500. GPCR_3_9-Cys_dom. IPR017978. GPCR_3_C. IPR017979. GPCR_3_CS. [Graphical view]
Pfam	PF00003. 7tm_3. 1 hit. PF01094. ANF_receptor. 1 hit. PF07562. NCD3G. 1 hit. [Graphical view]
PRINTS	PR00248. GPCRMGR.
PROSITE	PS00979. G_PROTEIN_RECEP_F3_1. 1 hit. PS00980. G_PROTEIN_RECEP_F3_2. 1 hit. PS00981. G_PROTEIN_RECEP_F3_3. 1 hit. PS50259. G_PROTEIN_RECEP_F3_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281082.
SOURCE	Search...

Entry information

Entry name

CASR_MOUSE

Accession

Primary (citable) accession number: Q9QY96
Secondary accession number(s): G3UX06 , O08968, O88519, Q9QY95, Q9QZU8, Q9R1D6, Q9R1Y2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 3, 2012
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program