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Reviewed, UniProtKB/Swiss-Prot Q9QY78 (IKKB_RAT)

Last modified October 13, 2009. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Inhibitor of nuclear factor kappa-B kinase subunit beta
      Short name=I-kappa-B-kinase beta
      Short name=IkBKB
      Short name=IKK-beta
      Short name=IKK-B
    EC=2.7.11.10
Alternative name(s):
    I-kappa-B kinase 2
      Short name=IKK2
    Nuclear factor NF-kappa-B inhibitor kinase beta
      Short name=NFKBIKB
Gene names
Name: Ikbkb
Synonyms: Ikkb
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length757 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3 By similarity.

Catalytic activity

ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].

Subunit structure

Component of the I-kappa-B-kinase (IKK) core complex consisting of CHUK, IKBKB and IKBKG; probably four alpha/CHUK-beta/IKBKB dimers are associated with four gamma/IKBKG subunits. The IKK core complex seems to associate with regulatory or adapter proteins to form a IKK-signalosome holo-complex. Part of a complex composed of NCOA2, NCOA3, CHUK/IKKA, IKBKB, IKBKG and CREBBP. Part of a 70-90 kDa complex at least consisting of CHUK/IKKA, IKBKB, NFKBIA, RELA, IKBKAP and MAP3K14. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with TICAM1. Interacts with FAF1; the interaction disrupts the IKK complex formation. Interacts with ATM. Part of a ternary complex consisting of TANK, IKBKB and IKBKG. Interacts with NIBP; the interaction is direct By similarity.

Subcellular location

Cytoplasm.

Post-translational modification

Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

Contains 1 protein kinase domain.

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Binary interactions

With

Entry

#Exp.

IntAct

Notes

Snap23O703771EBI-1812464,EBI-1573765

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 757757Inhibitor of nuclear factor kappa-B kinase subunit beta
PRO_0000086015

Regions

Domain15 – 300286Protein kinase
Domain458 – 47922Leucine-zipper Potential
Nucleotide binding21 – 299ATP By similarity
Region737 – 7426NEMO-binding

Sites

Active site1451Proton acceptor By similarity
Binding site441ATP By similarity

Amino acid modifications

Modified residue231Phosphothreonine By similarity
Modified residue1771Phosphoserine By similarity
Modified residue1811Phosphoserine By similarity
Modified residue6701Phosphoserine; by autocatalysis By similarity
Modified residue6721Phosphoserine; by autocatalysis By similarity
Modified residue6751Phosphoserine; by autocatalysis By similarity
Modified residue6821Phosphoserine; by autocatalysis By similarity
Modified residue6891Phosphoserine; by autocatalysis By similarity
Modified residue6921Phosphoserine; by autocatalysis By similarity
Modified residue6971Phosphoserine; by autocatalysis By similarity
Modified residue7051Phosphoserine; by autocatalysis By similarity
Modified residue7331Phosphoserine; by autocatalysis By similarity
Modified residue7401Phosphoserine; by autocatalysis By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q9QY78-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 3AFFE46A7DF91F9C

FASTA75786,866
        10         20         30         40         50         60 
MSWSPSLPTQ TCGAWEMKER LGTGGFGNVI RWHNQVTGEQ IAIKQCRQEL SPKNRDRWCL 

        70         80         90        100        110        120 
EIQIMRRLNH PNVVAARDVP EGMQNLAPND LPLLAMEYCQ GGDLRRYLNQ FENCCGLREG 

       130        140        150        160        170        180 
AILTLLSDIA SALRYLHENR IIHRDLKPEN IVLQQGEKRL IHKIIDLGYA KELDQGSLCT 

       190        200        210        220        230        240 
SFVGTLQYLA PELLEQQKYT VTVDYWSFGT LAFECITGFR PFLPNWQPVQ WHSKVRQKSE 

       250        260        270        280        290        300 
VDIVVSEDLN GTVKFSSSSP FPNNLNSVLA ERLEKWLQLM LTWQPRQRGV DPQYGPNGCF 

       310        320        330        340        350        360 
RALDDILNLK LVHILNMVTG TIHTYPVMED ESLQSLKTRI REDTGILETD QELLQEAGLV 

       370        380        390        400        410        420 
LLPDKPATQC ISDSKTNEGL TLDMDLVFLF DNSKMSYETQ ITPRPQPESV SCVLQEPKRN 

       430        440        450        460        470        480 
LSFFQMRKVW GQVWHSIQTL KEDCNRLQQG QRAAMMNLLR NNSCLSKMKN AMASTAQQLK 

       490        500        510        520        530        540 
AKLDFFKTSI QIDLEKYREQ TEFGITSDKL LLAWREMEQA VEQCGRENDV KVLVERMMAL 

       550        560        570        580        590        600 
QTDIVDLQRS PMGRKQGGTL DDLEEQAREL YRRLREKPRD QRTEGDSQDM VRLLLQAIQS 

       610        620        630        640        650        660 
FEKKVRVIYS QLSKTVVCKQ KALELLPKVE EVVRLMNEDE KTVVRLQEKR QKELWNLLKI 

       670        680        690        700        710        720 
ACSKVRGPVS GSPDSMNVSR LSHPGHLMSQ PSSACDSLPD SDKKSEELVA EAHALCSRLE 

       730        740        750 
SALQDTVKQQ DRSFTTLDWS WLQMEDEERC GLEQACD

« Hide

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References

[1]"IKK beta in megakaryocyte differentiation."
Zhang Y., Sun S., Ravid K.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Coordinate regulation of IkappaB kinases by mitogen-activated protein kinase kinase kinase 1 and NF-kappaB-inducing kinase."
Nemoto S., DiDonato J.A., Lin A.
Mol. Cell. Biol. 18:7336-7343(1998) [PubMed: 9819420] [Abstract]
Cited for: IKK PHOSPHORYLATION.
[3]"The p62 scaffold regulates nerve growth factor-induced NF-kappaB activation by influencing TRAF6 polyubiquitination."
Wooten M.W., Geetha T., Seibenhener M.L., Babu J.R., Diaz-Meco M.T., Moscat J.
J. Biol. Chem. 280:35625-35629(2005) [PubMed: 16079148] [Abstract]
Cited for: INTERACTION WITH SQSTM1; PRKCI AND TRAF6.
[4]"The I kappa B/NF-kappa B system: a key determinant of mucosal inflammation and protection."
Jobin C., Sartor R.B.
Am. J. Physiol. 278:C451-C462(2000) [PubMed: 10712233] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AF115282 mRNA. Translation: AAF21978.1.
IPIIPI00213608.
RefSeqNP_445807.2.
UniGeneRn.19222

3D structure databases

HSSPHSSP built from PDB template 1O6Y based on UniProtKB P71584.
ModBaseSearch...

Protein-protein interaction databases

IntActQ9QY78. 3 interactions.
STRINGQ9QY78.

PTM databases

PhosphoSiteQ9QY78.

Genome annotation databases

EnsemblENSRNOT00000025851; ENSRNOP00000025851; ENSRNOG00000019073; Rattus norvegicus. [Genome view]
GeneID84351.
KEGGrno:84351.
UCSCNM_053355. rat.

Organism-specific databases

CTD84351.
RGD621375. Ikbkb.

Phylogenomic databases

HOVERGENQ9QY78.

Enzyme and pathway databases

BRENDA2.7.11.10. 248.

Gene expression databases

ArrayExpressQ9QY78.
GenevestigatorQ9QY78.
GermOnlineENSRNOG00000019073. Rattus norvegicus.

Family and domain databases

InterProIPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio616659.

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Entry information

Entry nameIKKB_RAT
AccessionPrimary (citable) accession number: Q9QY78
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 2000
Last modified: October 13, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents