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Protein

Deoxyribonuclease-2-beta

Gene

Dnase2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes DNA under acidic conditions. Does not require divalent cations for activity. Participates in the degradation of nuclear DNA during lens cell differentiation.2 Publications

Catalytic activityi

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

GO - Molecular functioni

  • deoxyribonuclease II activity Source: GO_Central
  • endodeoxyribonuclease activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BRENDAi3.1.22.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyribonuclease-2-beta (EC:3.1.22.1)
Alternative name(s):
DNase II-like acid DNase
DNase2-like acid DNase
Deoxyribonuclease II beta
Short name:
DNase II beta
Endonuclease DLAD
Gene namesi
Name:Dnase2b
Synonyms:Dlad
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1913283. Dnase2b.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • extracellular region Source: MGI
  • lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000000729623 – 354Deoxyribonuclease-2-betaAdd BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi70N-linked (GlcNAc...)Sequence analysis1
Glycosylationi77N-linked (GlcNAc...)Sequence analysis1
Glycosylationi95N-linked (GlcNAc...)Sequence analysis1
Glycosylationi98N-linked (GlcNAc...)Sequence analysis1
Glycosylationi114N-linked (GlcNAc...)Sequence analysis1
Glycosylationi129N-linked (GlcNAc...)Sequence analysis1
Glycosylationi208N-linked (GlcNAc...)Sequence analysis1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi319N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9QY48.
PRIDEiQ9QY48.

PTM databases

PhosphoSitePlusiQ9QY48.

Expressioni

Tissue specificityi

Highly expressed in the eye lens. Detected in liver, but not in the other tissues tested.2 Publications

Gene expression databases

BgeeiENSMUSG00000028185.
CleanExiMM_DNASE2B.
GenevisibleiQ9QY48. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029836.

Structurei

3D structure databases

ProteinModelPortaliQ9QY48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DNase II family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3825. Eukaryota.
ENOG4111G8U. LUCA.
GeneTreeiENSGT00390000002634.
HOGENOMiHOG000261682.
HOVERGENiHBG051387.
InParanoidiQ9QY48.
KOiK01158.
OMAiQDHAKWC.
OrthoDBiEOG091G08N7.
PhylomeDBiQ9QY48.
TreeFamiTF314536.

Family and domain databases

InterProiIPR004947. DNase_II.
[Graphical view]
PANTHERiPTHR10858. PTHR10858. 1 hit.
PfamiPF03265. DNase_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QY48-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAKPLRTVL SLLFFALSGV LGTPEISCRN EYGEAVDWFI FYKLPKRTSK
60 70 80 90 100
ASEEAGLQYL YLDSTRQTWN KSLYLINSTR SALGRTLQHL YDTHNSTNDT
110 120 130 140 150
AYLIYNDGVP GSVNYSRQYG HAKGLLVWNR TQGFWLIHSV PKFPPVHGYE
160 170 180 190 200
YPTSGRRYGQ TGICITFGYS QFEEIDFQLL VLQPNIYSCF IPSTFHWKLI
210 220 230 240 250
YMPRMCANSS SLKIPVRYLA ELHSAQGLNF VHFAKSSFYT DDIFTGWIAQ
260 270 280 290 300
KLKTHLLAQT WQKKKQELPS NCSLPYHVYN IKSIGVTSKS YFSSRQDHSK
310 320 330 340 350
WCVSIKGSAN RWTCIGDLNR SLHQALRGGG FICTKNHYIY QAFHKLYLRY

GFCK
Length:354
Mass (Da):40,794
Last modified:May 1, 2000 - v1
Checksum:i40A5DD6E9AD278A1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85R → K in BAC37579 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128888 mRNA. Translation: AAF05082.1.
AF334608
, AF334603, AF334604, AF334605, AF334606, AF334607 Genomic DNA. Translation: AAL34450.1.
AK079224 mRNA. Translation: BAC37579.1.
BC075661 mRNA. Translation: AAH75661.1.
CCDSiCCDS17904.1.
RefSeqiNP_064341.3. NM_019957.4.
XP_006501826.1. XM_006501763.3.
XP_006501827.1. XM_006501764.2.
XP_011238473.1. XM_011240171.1.
XP_011238474.1. XM_011240172.1.
UniGeneiMm.25853.

Genome annotation databases

EnsembliENSMUST00000029836; ENSMUSP00000029836; ENSMUSG00000028185.
ENSMUST00000200633; ENSMUSP00000142872; ENSMUSG00000028185.
GeneIDi56629.
KEGGimmu:56629.
UCSCiuc008rrn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF128888 mRNA. Translation: AAF05082.1.
AF334608
, AF334603, AF334604, AF334605, AF334606, AF334607 Genomic DNA. Translation: AAL34450.1.
AK079224 mRNA. Translation: BAC37579.1.
BC075661 mRNA. Translation: AAH75661.1.
CCDSiCCDS17904.1.
RefSeqiNP_064341.3. NM_019957.4.
XP_006501826.1. XM_006501763.3.
XP_006501827.1. XM_006501764.2.
XP_011238473.1. XM_011240171.1.
XP_011238474.1. XM_011240172.1.
UniGeneiMm.25853.

3D structure databases

ProteinModelPortaliQ9QY48.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029836.

PTM databases

PhosphoSitePlusiQ9QY48.

Proteomic databases

PaxDbiQ9QY48.
PRIDEiQ9QY48.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029836; ENSMUSP00000029836; ENSMUSG00000028185.
ENSMUST00000200633; ENSMUSP00000142872; ENSMUSG00000028185.
GeneIDi56629.
KEGGimmu:56629.
UCSCiuc008rrn.2. mouse.

Organism-specific databases

CTDi58511.
MGIiMGI:1913283. Dnase2b.

Phylogenomic databases

eggNOGiKOG3825. Eukaryota.
ENOG4111G8U. LUCA.
GeneTreeiENSGT00390000002634.
HOGENOMiHOG000261682.
HOVERGENiHBG051387.
InParanoidiQ9QY48.
KOiK01158.
OMAiQDHAKWC.
OrthoDBiEOG091G08N7.
PhylomeDBiQ9QY48.
TreeFamiTF314536.

Enzyme and pathway databases

BRENDAi3.1.22.1. 3474.

Miscellaneous databases

PROiQ9QY48.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028185.
CleanExiMM_DNASE2B.
GenevisibleiQ9QY48. MM.

Family and domain databases

InterProiIPR004947. DNase_II.
[Graphical view]
PANTHERiPTHR10858. PTHR10858. 1 hit.
PfamiPF03265. DNase_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDNS2B_MOUSE
AccessioniPrimary (citable) accession number: Q9QY48
Secondary accession number(s): Q8C589
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibited by aurintricarboxylic acid and Zn2+.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.