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Q9QY17 (PACN2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein kinase C and casein kinase substrate in neurons 2 protein
Alternative name(s):
Synaptic dynamin-associated protein II
Syndapin-2
Syndapin-II
Short name=SdpII
Gene names
Name:Pacsin2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length488 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis. Ref.1

Subunit structure

Homodimer. May form heterooligomers with other PACSINs. Interacts (via NPF motifs) with EHD1 (via EH domain). Interacts with EHD3. Interacts (via the SH3 domain) with MICALL1. Interacts with RAC1. Interacts (via SH3 domain) with DNM1, SYN1, SYNJ1 and WASL. Interacts with CAV1. Interacts with TRPV4. Ref.1

Subcellular location

Cytoplasm. Cytoplasmcytoskeleton By similarity. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projectionruffle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Early endosome By similarity. Recycling endosome membrane By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell projection By similarity. Membranecaveola By similarity. Note: Detected at the neck of flask-shaped caveolae. Localization to tubular recycling endosomes probably requires interaction with MICALL1 and EHD1. Ref.1

Tissue specificity

Widely expressed (at protein level). Isoforms 1/3 are predominantly expressed in heart and in PC-12 cells, a pheochromocytoma cell line (at protein level). Isoforms 2/4 are widely expressed with highest levels in muscle, testis and brain (at protein level). Ref.1

Domain

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain By similarity. Endocytic vesicle-like distribution.

Post-translational modification

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC) By similarity.

Sequence similarities

Belongs to the PACSIN family.

Contains 1 FCH domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Biological processEndocytosis
   Cellular componentCell membrane
Cell projection
Cytoplasm
Cytoplasmic vesicle
Cytoskeleton
Endosome
Membrane
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
SH3 domain
   LigandLipid-binding
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin cytoskeleton organization

Inferred from electronic annotation. Source: InterPro

caveola assembly

Inferred from sequence or structural similarity. Source: UniProtKB

caveolin-mediated endocytosis

Inferred from sequence or structural similarity. Source: UniProtKB

cell projection morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

membrane tubulation

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentcaveola

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

early endosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

extrinsic component of membrane

Inferred from sequence or structural similarity. Source: UniProtKB

recycling endosome membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

ruffle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphosphatidic acid binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein binding

Inferred from physical interaction PubMed 15371016PubMed 16357825PubMed 16999739PubMed 21725280. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CoblQ5NBX14EBI-491201,EBI-1550138From a different organism.
Ehd1Q641Z62EBI-491201,EBI-492911
Ehd4Q9EQP24EBI-491201,EBI-491022From a different organism.
NCKIPSDQ9NZQ32EBI-491201,EBI-745080From a different organism.
PDE6GP185454EBI-491201,EBI-2622029From a different organism.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9QY17-1)

Also known as: Aa; SdpII-l;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9QY17-2)

Also known as: Ab;

The sequence of this isoform differs from the canonical sequence as follows:
     346-386: Missing.
Isoform 3 (identifier: Q9QY17-3)

Also known as: Ba;

The sequence of this isoform differs from the canonical sequence as follows:
     302-303: Missing.
Isoform 4 (identifier: Q9QY17-4)

Also known as: Bb; SdpII-s;

The sequence of this isoform differs from the canonical sequence as follows:
     302-303: Missing.
     346-386: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 488488Protein kinase C and casein kinase substrate in neurons 2 protein
PRO_0000161797

Regions

Domain11 – 7565FCH
Domain428 – 48861SH3
Region1 – 308308F-BAR domain By similarity
Coiled coil25 – 274250 By similarity
Motif364 – 3663NPF1
Motif407 – 4093NPF2
Motif419 – 4213NPF3

Amino acid modifications

Modified residue531N6-acetyllysine By similarity

Natural variations

Alternative sequence302 – 3032Missing in isoform 3 and isoform 4.
VSP_004518
Alternative sequence346 – 38641Missing in isoform 2 and isoform 4.
VSP_004519

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Aa) (SdpII-l) [UniParc].

Last modified August 13, 2002. Version 2.
Checksum: B2975012EF0FDF56

FASTA48855,978
        10         20         30         40         50         60 
MSVTYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCGD LMNCLHERAR IEKAYAQQLT 

        70         80         90        100        110        120 
EWARRWRQLV EKGPQYGTVE KAWMAVMSEA ERVSELHLEV KASLMNEDFE KIKNWQKEAF 

       130        140        150        160        170        180 
HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK EVDAAKKAHH TACKEEKLAV SREANSKADP 

       190        200        210        220        230        240 
SLNPEQLKKL QDKIEKCKQD VLKTKDKYEK ALKELDQTTP QYMENMEQVF EQCQQFEEKR 

       250        260        270        280        290        300 
LRFFREVLLE VQKHLDLSNV ASYKGIYREL EQSIKAADAV EDLRWFRANH GPGMAMNWPQ 

       310        320        330        340        350        360 
FEDEEWSADL NRTLSRREKK KAADGVTLTG INQTGDQSGQ NKPSSNLSVP SNPAQSTQLQ 

       370        380        390        400        410        420 
SSYNPFEDED DTGSSVSEKE DIKAKNVSSY EKTQNYPADW SDDESNNPFS STDANGDSNP 

       430        440        450        460        470        480 
FDEDTTSGTE VRVRALYDYE GQEHDELSFK AGDELTKIED EDEQGWCKGR LDSGQVGLYP 


ANYVEAIQ 

« Hide

Isoform 2 (Ab) [UniParc].

Checksum: B202B81D7D48E6D1
Show »

FASTA44751,538
Isoform 3 (Ba) [UniParc].

Checksum: B140F324EF11E29B
Show »

FASTA48655,734
Isoform 4 (Bb) (SdpII-s) [UniParc].

Checksum: 99512F063951F4A6
Show »

FASTA44551,294

References

[1]"Syndapin isoforms participate in receptor-mediated endocytosis and actin organization."
Qualmann B., Kelly R.B.
J. Cell Biol. 148:1047-1062(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), FUNCTION, INTERACTION WITH DNM1; SYN1; SYNJ1 AND WASL, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF139492 mRNA. Translation: AAF22211.1.
AF139493 mRNA. Translation: AAF22212.1.
AF139494 mRNA. Translation: AAF22213.1.
AF139495 mRNA. Translation: AAF22214.1.
RefSeqNP_570096.2. NM_130740.2. [Q9QY17-3]
XP_006242112.1. XM_006242050.1. [Q9QY17-1]
XP_006242113.1. XM_006242051.1. [Q9QY17-1]
XP_006242114.1. XM_006242052.1. [Q9QY17-1]
XP_006242115.1. XM_006242053.1. [Q9QY17-1]
XP_006242117.1. XM_006242055.1. [Q9QY17-3]
XP_006242119.1. XM_006242057.1. [Q9QY17-2]
XP_006242120.1. XM_006242058.1. [Q9QY17-2]
XP_006242121.1. XM_006242059.1. [Q9QY17-4]
XP_006242122.1. XM_006242060.1. [Q9QY17-4]
UniGeneRn.17106.

3D structure databases

ProteinModelPortalQ9QY17.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9QY17. 5 interactions.
MINTMINT-4567486.
STRING10116.ENSRNOP00000013398.

PTM databases

PhosphoSiteQ9QY17.

Proteomic databases

PaxDbQ9QY17.
PRIDEQ9QY17.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000013398; ENSRNOP00000013398; ENSRNOG00000009756. [Q9QY17-3]
GeneID124461.
KEGGrno:124461.

Organism-specific databases

CTD11252.
RGD69411. Pacsin2.

Phylogenomic databases

eggNOGNOG283356.
GeneTreeENSGT00510000046376.
HOGENOMHOG000007245.
HOVERGENHBG053486.
InParanoidQ9QY17.
OrthoDBEOG75TMBJ.
PhylomeDBQ9QY17.

Gene expression databases

GenevestigatorQ9QY17.

Family and domain databases

InterProIPR001060. FCH_dom.
IPR028521. PACSIN2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR10959:SF2. PTHR10959:SF2. 1 hit.
PfamPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio620467.
PROQ9QY17.

Entry information

Entry namePACN2_RAT
AccessionPrimary (citable) accession number: Q9QY17
Secondary accession number(s): Q9QY18, Q9QY19, Q9QY20
Entry history
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: August 13, 2002
Last modified: June 11, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families