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Q9QY17

- PACN2_RAT

UniProt

Q9QY17 - PACN2_RAT

Protein

Protein kinase C and casein kinase substrate in neurons 2 protein

Gene

Pacsin2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (13 Aug 2002)
      Previous versions | rss
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    Functioni

    Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.1 Publication

    GO - Molecular functioni

    1. phosphatidic acid binding Source: UniProtKB
    2. protein binding Source: IntAct

    GO - Biological processi

    1. actin cytoskeleton organization Source: InterPro
    2. caveola assembly Source: UniProtKB
    3. caveolin-mediated endocytosis Source: UniProtKB
    4. cell projection morphogenesis Source: UniProtKB
    5. membrane tubulation Source: UniProtKB

    Keywords - Biological processi

    Endocytosis

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein kinase C and casein kinase substrate in neurons 2 protein
    Alternative name(s):
    Synaptic dynamin-associated protein II
    Syndapin-2
    Syndapin-II
    Short name:
    SdpII
    Gene namesi
    Name:Pacsin2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi69411. Pacsin2.

    Subcellular locationi

    Cytoplasm 1 Publication. Cytoplasmcytoskeleton By similarity. Cytoplasmic vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication. Cell projectionruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Early endosome By similarity. Recycling endosome membrane By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity. Cell projection By similarity. Membranecaveola By similarity
    Note: Detected at the neck of flask-shaped caveolae. Localization to tubular recycling endosomes probably requires interaction with MICALL1 and EHD1.

    GO - Cellular componenti

    1. caveola Source: UniProtKB-SubCell
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. cytoskeleton Source: UniProtKB-SubCell
    4. early endosome Source: UniProtKB-SubCell
    5. extrinsic component of membrane Source: UniProtKB
    6. recycling endosome membrane Source: UniProtKB-SubCell
    7. ruffle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 488488Protein kinase C and casein kinase substrate in neurons 2 proteinPRO_0000161797Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei53 – 531N6-acetyllysineBy similarity

    Post-translational modificationi

    Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiQ9QY17.
    PRIDEiQ9QY17.

    PTM databases

    PhosphoSiteiQ9QY17.

    Expressioni

    Tissue specificityi

    Widely expressed (at protein level). Isoforms 1/3 are predominantly expressed in heart and in PC-12 cells, a pheochromocytoma cell line (at protein level). Isoforms 2/4 are widely expressed with highest levels in muscle, testis and brain (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriQ9QY17.

    Interactioni

    Subunit structurei

    Homodimer. May form heterooligomers with other PACSINs. Interacts (via NPF motifs) with EHD1 (via EH domain). Interacts with EHD3. Interacts (via the SH3 domain) with MICALL1. Interacts with RAC1. Interacts (via SH3 domain) with DNM1, SYN1, SYNJ1 and WASL. Interacts with CAV1. Interacts with TRPV4.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    CoblQ5NBX14EBI-491201,EBI-1550138From a different organism.
    Ehd1Q641Z62EBI-491201,EBI-492911
    Ehd4Q9EQP24EBI-491201,EBI-491022From a different organism.
    NCKIPSDQ9NZQ32EBI-491201,EBI-745080From a different organism.
    PDE6GP185454EBI-491201,EBI-2622029From a different organism.

    Protein-protein interaction databases

    IntActiQ9QY17. 5 interactions.
    MINTiMINT-4567486.
    STRINGi10116.ENSRNOP00000013398.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QY17.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini11 – 7565FCHPROSITE-ProRule annotationAdd
    BLAST
    Domaini428 – 48861SH3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 308308F-BAR domainBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili25 – 274250By similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi364 – 3663NPF1
    Motifi407 – 4093NPF2
    Motifi419 – 4213NPF3

    Domaini

    The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain By similarity. Endocytic vesicle-like distribution.By similarity

    Sequence similaritiesi

    Belongs to the PACSIN family.Curated
    Contains 1 FCH domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG283356.
    GeneTreeiENSGT00510000046376.
    HOGENOMiHOG000007245.
    HOVERGENiHBG053486.
    InParanoidiQ9QY17.
    OrthoDBiEOG75TMBJ.
    PhylomeDBiQ9QY17.

    Family and domain databases

    InterProiIPR001060. FCH_dom.
    IPR028521. PACSIN2.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR10959:SF2. PTHR10959:SF2. 1 hit.
    PfamiPF00611. FCH. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9QY17-1) [UniParc]FASTAAdd to Basket

    Also known as: Aa, SdpII-l

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSVTYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCGD LMNCLHERAR    50
    IEKAYAQQLT EWARRWRQLV EKGPQYGTVE KAWMAVMSEA ERVSELHLEV 100
    KASLMNEDFE KIKNWQKEAF HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK 150
    EVDAAKKAHH TACKEEKLAV SREANSKADP SLNPEQLKKL QDKIEKCKQD 200
    VLKTKDKYEK ALKELDQTTP QYMENMEQVF EQCQQFEEKR LRFFREVLLE 250
    VQKHLDLSNV ASYKGIYREL EQSIKAADAV EDLRWFRANH GPGMAMNWPQ 300
    FEDEEWSADL NRTLSRREKK KAADGVTLTG INQTGDQSGQ NKPSSNLSVP 350
    SNPAQSTQLQ SSYNPFEDED DTGSSVSEKE DIKAKNVSSY EKTQNYPADW 400
    SDDESNNPFS STDANGDSNP FDEDTTSGTE VRVRALYDYE GQEHDELSFK 450
    AGDELTKIED EDEQGWCKGR LDSGQVGLYP ANYVEAIQ 488
    Length:488
    Mass (Da):55,978
    Last modified:August 13, 2002 - v2
    Checksum:iB2975012EF0FDF56
    GO
    Isoform 2 (identifier: Q9QY17-2) [UniParc]FASTAAdd to Basket

    Also known as: Ab

    The sequence of this isoform differs from the canonical sequence as follows:
         346-386: Missing.

    Show »
    Length:447
    Mass (Da):51,538
    Checksum:iB202B81D7D48E6D1
    GO
    Isoform 3 (identifier: Q9QY17-3) [UniParc]FASTAAdd to Basket

    Also known as: Ba

    The sequence of this isoform differs from the canonical sequence as follows:
         302-303: Missing.

    Show »
    Length:486
    Mass (Da):55,734
    Checksum:iB140F324EF11E29B
    GO
    Isoform 4 (identifier: Q9QY17-4) [UniParc]FASTAAdd to Basket

    Also known as: Bb, SdpII-s

    The sequence of this isoform differs from the canonical sequence as follows:
         302-303: Missing.
         346-386: Missing.

    Show »
    Length:445
    Mass (Da):51,294
    Checksum:i99512F063951F4A6
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei302 – 3032Missing in isoform 3 and isoform 4. 1 PublicationVSP_004518
    Alternative sequencei346 – 38641Missing in isoform 2 and isoform 4. 1 PublicationVSP_004519Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF139492 mRNA. Translation: AAF22211.1.
    AF139493 mRNA. Translation: AAF22212.1.
    AF139494 mRNA. Translation: AAF22213.1.
    AF139495 mRNA. Translation: AAF22214.1.
    RefSeqiNP_570096.2. NM_130740.2. [Q9QY17-3]
    XP_006242112.1. XM_006242050.1. [Q9QY17-1]
    XP_006242113.1. XM_006242051.1. [Q9QY17-1]
    XP_006242114.1. XM_006242052.1. [Q9QY17-1]
    XP_006242115.1. XM_006242053.1. [Q9QY17-1]
    XP_006242117.1. XM_006242055.1. [Q9QY17-3]
    XP_006242119.1. XM_006242057.1. [Q9QY17-2]
    XP_006242120.1. XM_006242058.1. [Q9QY17-2]
    XP_006242121.1. XM_006242059.1. [Q9QY17-4]
    XP_006242122.1. XM_006242060.1. [Q9QY17-4]
    UniGeneiRn.17106.

    Genome annotation databases

    EnsembliENSRNOT00000013398; ENSRNOP00000013398; ENSRNOG00000009756. [Q9QY17-3]
    GeneIDi124461.
    KEGGirno:124461.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF139492 mRNA. Translation: AAF22211.1 .
    AF139493 mRNA. Translation: AAF22212.1 .
    AF139494 mRNA. Translation: AAF22213.1 .
    AF139495 mRNA. Translation: AAF22214.1 .
    RefSeqi NP_570096.2. NM_130740.2. [Q9QY17-3 ]
    XP_006242112.1. XM_006242050.1. [Q9QY17-1 ]
    XP_006242113.1. XM_006242051.1. [Q9QY17-1 ]
    XP_006242114.1. XM_006242052.1. [Q9QY17-1 ]
    XP_006242115.1. XM_006242053.1. [Q9QY17-1 ]
    XP_006242117.1. XM_006242055.1. [Q9QY17-3 ]
    XP_006242119.1. XM_006242057.1. [Q9QY17-2 ]
    XP_006242120.1. XM_006242058.1. [Q9QY17-2 ]
    XP_006242121.1. XM_006242059.1. [Q9QY17-4 ]
    XP_006242122.1. XM_006242060.1. [Q9QY17-4 ]
    UniGenei Rn.17106.

    3D structure databases

    ProteinModelPortali Q9QY17.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9QY17. 5 interactions.
    MINTi MINT-4567486.
    STRINGi 10116.ENSRNOP00000013398.

    PTM databases

    PhosphoSitei Q9QY17.

    Proteomic databases

    PaxDbi Q9QY17.
    PRIDEi Q9QY17.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000013398 ; ENSRNOP00000013398 ; ENSRNOG00000009756 . [Q9QY17-3 ]
    GeneIDi 124461.
    KEGGi rno:124461.

    Organism-specific databases

    CTDi 11252.
    RGDi 69411. Pacsin2.

    Phylogenomic databases

    eggNOGi NOG283356.
    GeneTreei ENSGT00510000046376.
    HOGENOMi HOG000007245.
    HOVERGENi HBG053486.
    InParanoidi Q9QY17.
    OrthoDBi EOG75TMBJ.
    PhylomeDBi Q9QY17.

    Miscellaneous databases

    NextBioi 620467.
    PROi Q9QY17.

    Gene expression databases

    Genevestigatori Q9QY17.

    Family and domain databases

    InterProi IPR001060. FCH_dom.
    IPR028521. PACSIN2.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR10959:SF2. PTHR10959:SF2. 1 hit.
    Pfami PF00611. FCH. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Syndapin isoforms participate in receptor-mediated endocytosis and actin organization."
      Qualmann B., Kelly R.B.
      J. Cell Biol. 148:1047-1062(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), FUNCTION, INTERACTION WITH DNM1; SYN1; SYNJ1 AND WASL, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Brain.

    Entry informationi

    Entry nameiPACN2_RAT
    AccessioniPrimary (citable) accession number: Q9QY17
    Secondary accession number(s): Q9QY18, Q9QY19, Q9QY20
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 2002
    Last sequence update: August 13, 2002
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3