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Protein

Protein kinase C and casein kinase substrate in neurons 2 protein

Gene

Pacsin2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase substrate in neurons 2 protein
Alternative name(s):
Synaptic dynamin-associated protein II
Syndapin-2
Syndapin-II
Short name:
SdpII
Gene namesi
Name:Pacsin2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi69411. Pacsin2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Protein kinase C and casein kinase substrate in neurons 2 proteinPRO_0000161797Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9QY17.
PRIDEiQ9QY17.

PTM databases

PhosphoSiteiQ9QY17.

Expressioni

Tissue specificityi

Widely expressed (at protein level). Isoforms 1/3 are predominantly expressed in heart and in PC-12 cells, a pheochromocytoma cell line (at protein level). Isoforms 2/4 are widely expressed with highest levels in muscle, testis and brain (at protein level).1 Publication

Gene expression databases

ExpressionAtlasiQ9QY17. baseline and differential.
GenevisibleiQ9QY17. RN.

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts (via NPF motifs) with EHD1 (via EH domain). Interacts with EHD3. Interacts (via the SH3 domain) with MICALL1. Interacts with RAC1. Interacts (via SH3 domain) with DNM1, SYN1, SYNJ1 and WASL. Interacts with CAV1. Interacts with TRPV4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CoblQ5NBX14EBI-491201,EBI-1550138From a different organism.
Ehd1Q641Z62EBI-491201,EBI-492911
Ehd4Q9EQP24EBI-491201,EBI-491022From a different organism.
NCKIPSDQ9NZQ32EBI-491201,EBI-745080From a different organism.
PDE6GP185454EBI-491201,EBI-2622029From a different organism.

Protein-protein interaction databases

IntActiQ9QY17. 5 interactions.
MINTiMINT-4567486.
STRINGi10116.ENSRNOP00000060074.

Structurei

3D structure databases

ProteinModelPortaliQ9QY17.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 282272F-BARPROSITE-ProRule annotationAdd
BLAST
Domaini428 – 48861SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili25 – 274250By similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi364 – 3663NPF1
Motifi407 – 4093NPF2
Motifi419 – 4213NPF3

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain (By similarity). Endocytic vesicle-like distribution.By similarity

Sequence similaritiesi

Belongs to the PACSIN family.Curated
Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG283356.
GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiQ9QY17.
OrthoDBiEOG75TMBJ.
PhylomeDBiQ9QY17.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR028521. PACSIN2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10959:SF2. PTHR10959:SF2. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QY17-1) [UniParc]FASTAAdd to basket

Also known as: Aa, SdpII-l

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVTYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCGD LMNCLHERAR
60 70 80 90 100
IEKAYAQQLT EWARRWRQLV EKGPQYGTVE KAWMAVMSEA ERVSELHLEV
110 120 130 140 150
KASLMNEDFE KIKNWQKEAF HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK
160 170 180 190 200
EVDAAKKAHH TACKEEKLAV SREANSKADP SLNPEQLKKL QDKIEKCKQD
210 220 230 240 250
VLKTKDKYEK ALKELDQTTP QYMENMEQVF EQCQQFEEKR LRFFREVLLE
260 270 280 290 300
VQKHLDLSNV ASYKGIYREL EQSIKAADAV EDLRWFRANH GPGMAMNWPQ
310 320 330 340 350
FEDEEWSADL NRTLSRREKK KAADGVTLTG INQTGDQSGQ NKPSSNLSVP
360 370 380 390 400
SNPAQSTQLQ SSYNPFEDED DTGSSVSEKE DIKAKNVSSY EKTQNYPADW
410 420 430 440 450
SDDESNNPFS STDANGDSNP FDEDTTSGTE VRVRALYDYE GQEHDELSFK
460 470 480
AGDELTKIED EDEQGWCKGR LDSGQVGLYP ANYVEAIQ
Length:488
Mass (Da):55,978
Last modified:August 13, 2002 - v2
Checksum:iB2975012EF0FDF56
GO
Isoform 2 (identifier: Q9QY17-2) [UniParc]FASTAAdd to basket

Also known as: Ab

The sequence of this isoform differs from the canonical sequence as follows:
     346-386: Missing.

Show »
Length:447
Mass (Da):51,538
Checksum:iB202B81D7D48E6D1
GO
Isoform 3 (identifier: Q9QY17-3) [UniParc]FASTAAdd to basket

Also known as: Ba

The sequence of this isoform differs from the canonical sequence as follows:
     302-303: Missing.

Show »
Length:486
Mass (Da):55,734
Checksum:iB140F324EF11E29B
GO
Isoform 4 (identifier: Q9QY17-4) [UniParc]FASTAAdd to basket

Also known as: Bb, SdpII-s

The sequence of this isoform differs from the canonical sequence as follows:
     302-303: Missing.
     346-386: Missing.

Show »
Length:445
Mass (Da):51,294
Checksum:i99512F063951F4A6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei302 – 3032Missing in isoform 3 and isoform 4. 1 PublicationVSP_004518
Alternative sequencei346 – 38641Missing in isoform 2 and isoform 4. 1 PublicationVSP_004519Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139492 mRNA. Translation: AAF22211.1.
AF139493 mRNA. Translation: AAF22212.1.
AF139494 mRNA. Translation: AAF22213.1.
AF139495 mRNA. Translation: AAF22214.1.
RefSeqiNP_570096.2. NM_130740.2. [Q9QY17-3]
XP_006242112.1. XM_006242050.2. [Q9QY17-1]
XP_006242113.1. XM_006242051.1. [Q9QY17-1]
XP_006242114.1. XM_006242052.2. [Q9QY17-1]
XP_006242115.1. XM_006242053.1. [Q9QY17-1]
XP_006242117.1. XM_006242055.1. [Q9QY17-3]
XP_006242119.1. XM_006242057.2. [Q9QY17-2]
XP_006242120.1. XM_006242058.1. [Q9QY17-2]
XP_006242121.1. XM_006242059.2. [Q9QY17-4]
XP_006242122.1. XM_006242060.1. [Q9QY17-4]
UniGeneiRn.17106.

Genome annotation databases

EnsembliENSRNOT00000013398; ENSRNOP00000013398; ENSRNOG00000009756. [Q9QY17-3]
GeneIDi124461.
KEGGirno:124461.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139492 mRNA. Translation: AAF22211.1.
AF139493 mRNA. Translation: AAF22212.1.
AF139494 mRNA. Translation: AAF22213.1.
AF139495 mRNA. Translation: AAF22214.1.
RefSeqiNP_570096.2. NM_130740.2. [Q9QY17-3]
XP_006242112.1. XM_006242050.2. [Q9QY17-1]
XP_006242113.1. XM_006242051.1. [Q9QY17-1]
XP_006242114.1. XM_006242052.2. [Q9QY17-1]
XP_006242115.1. XM_006242053.1. [Q9QY17-1]
XP_006242117.1. XM_006242055.1. [Q9QY17-3]
XP_006242119.1. XM_006242057.2. [Q9QY17-2]
XP_006242120.1. XM_006242058.1. [Q9QY17-2]
XP_006242121.1. XM_006242059.2. [Q9QY17-4]
XP_006242122.1. XM_006242060.1. [Q9QY17-4]
UniGeneiRn.17106.

3D structure databases

ProteinModelPortaliQ9QY17.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QY17. 5 interactions.
MINTiMINT-4567486.
STRINGi10116.ENSRNOP00000060074.

PTM databases

PhosphoSiteiQ9QY17.

Proteomic databases

PaxDbiQ9QY17.
PRIDEiQ9QY17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000013398; ENSRNOP00000013398; ENSRNOG00000009756. [Q9QY17-3]
GeneIDi124461.
KEGGirno:124461.

Organism-specific databases

CTDi11252.
RGDi69411. Pacsin2.

Phylogenomic databases

eggNOGiNOG283356.
GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiQ9QY17.
OrthoDBiEOG75TMBJ.
PhylomeDBiQ9QY17.

Miscellaneous databases

NextBioi620467.
PROiQ9QY17.

Gene expression databases

ExpressionAtlasiQ9QY17. baseline and differential.
GenevisibleiQ9QY17. RN.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR028521. PACSIN2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10959:SF2. PTHR10959:SF2. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Syndapin isoforms participate in receptor-mediated endocytosis and actin organization."
    Qualmann B., Kelly R.B.
    J. Cell Biol. 148:1047-1062(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), FUNCTION, INTERACTION WITH DNM1; SYN1; SYNJ1 AND WASL, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiPACN2_RAT
AccessioniPrimary (citable) accession number: Q9QY17
Secondary accession number(s): Q9QY18, Q9QY19, Q9QY20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: August 13, 2002
Last modified: June 24, 2015
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.