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Q9QY17

- PACN2_RAT

UniProt

Q9QY17 - PACN2_RAT

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Protein
Protein kinase C and casein kinase substrate in neurons 2 protein
Gene
Pacsin2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Lipid-binding protein that is able to promote the tubulation of the phosphatidic acid-containing membranes it preferentially binds. Plays a role in intracellular vesicle-mediated transport. Involved in the endocytosis of cell-surface receptors like the EGF receptor, contributing to its internalization in the absence of EGF stimulus. May also play a role in the formation of caveolae at the cell membrane. Recruits DNM2 to caveolae, and thereby plays a role in caveola-mediated endocytosis.1 Publication

GO - Molecular functioni

  1. phosphatidic acid binding Source: UniProtKB
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. actin cytoskeleton organization Source: InterPro
  2. caveola assembly Source: UniProtKB
  3. caveolin-mediated endocytosis Source: UniProtKB
  4. cell projection morphogenesis Source: UniProtKB
  5. membrane tubulation Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase substrate in neurons 2 protein
Alternative name(s):
Synaptic dynamin-associated protein II
Syndapin-2
Syndapin-II
Short name:
SdpII
Gene namesi
Name:Pacsin2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi69411. Pacsin2.

Subcellular locationi

Cytoplasm. Cytoplasmcytoskeleton By similarity. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projectionruffle membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Early endosome By similarity. Recycling endosome membrane By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Cell projection By similarity. Membranecaveola By similarity
Note: Detected at the neck of flask-shaped caveolae. Localization to tubular recycling endosomes probably requires interaction with MICALL1 and EHD1.1 Publication

GO - Cellular componenti

  1. caveola Source: UniProtKB-SubCell
  2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  3. cytoskeleton Source: UniProtKB-SubCell
  4. early endosome Source: UniProtKB-SubCell
  5. extrinsic component of membrane Source: UniProtKB
  6. recycling endosome membrane Source: UniProtKB-SubCell
  7. ruffle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Protein kinase C and casein kinase substrate in neurons 2 protein
PRO_0000161797Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei53 – 531N6-acetyllysine By similarity

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC) By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9QY17.
PRIDEiQ9QY17.

PTM databases

PhosphoSiteiQ9QY17.

Expressioni

Tissue specificityi

Widely expressed (at protein level). Isoforms 1/3 are predominantly expressed in heart and in PC-12 cells, a pheochromocytoma cell line (at protein level). Isoforms 2/4 are widely expressed with highest levels in muscle, testis and brain (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ9QY17.

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts (via NPF motifs) with EHD1 (via EH domain). Interacts with EHD3. Interacts (via the SH3 domain) with MICALL1. Interacts with RAC1. Interacts (via SH3 domain) with DNM1, SYN1, SYNJ1 and WASL. Interacts with CAV1. Interacts with TRPV4.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CoblQ5NBX14EBI-491201,EBI-1550138From a different organism.
Ehd1Q641Z62EBI-491201,EBI-492911
Ehd4Q9EQP24EBI-491201,EBI-491022From a different organism.
NCKIPSDQ9NZQ32EBI-491201,EBI-745080From a different organism.
PDE6GP185454EBI-491201,EBI-2622029From a different organism.

Protein-protein interaction databases

IntActiQ9QY17. 5 interactions.
MINTiMINT-4567486.
STRINGi10116.ENSRNOP00000013398.

Structurei

3D structure databases

ProteinModelPortaliQ9QY17.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 7565FCH
Add
BLAST
Domaini428 – 48861SH3
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 308308F-BAR domain By similarity
Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili25 – 274250 By similarity
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi364 – 3663NPF1
Motifi407 – 4093NPF2
Motifi419 – 4213NPF3

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain By similarity. Endocytic vesicle-like distribution.

Sequence similaritiesi

Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain.

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG283356.
GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiQ9QY17.
OrthoDBiEOG75TMBJ.
PhylomeDBiQ9QY17.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR028521. PACSIN2.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10959:SF2. PTHR10959:SF2. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9QY17-1) [UniParc]FASTAAdd to Basket

Also known as: Aa, SdpII-l

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MSVTYDDSVG VEVSSDSFWE VGNYKRTVKR IDDGHRLCGD LMNCLHERAR    50
IEKAYAQQLT EWARRWRQLV EKGPQYGTVE KAWMAVMSEA ERVSELHLEV 100
KASLMNEDFE KIKNWQKEAF HKQMMGGFKE TKEAEDGFRK AQKPWAKKLK 150
EVDAAKKAHH TACKEEKLAV SREANSKADP SLNPEQLKKL QDKIEKCKQD 200
VLKTKDKYEK ALKELDQTTP QYMENMEQVF EQCQQFEEKR LRFFREVLLE 250
VQKHLDLSNV ASYKGIYREL EQSIKAADAV EDLRWFRANH GPGMAMNWPQ 300
FEDEEWSADL NRTLSRREKK KAADGVTLTG INQTGDQSGQ NKPSSNLSVP 350
SNPAQSTQLQ SSYNPFEDED DTGSSVSEKE DIKAKNVSSY EKTQNYPADW 400
SDDESNNPFS STDANGDSNP FDEDTTSGTE VRVRALYDYE GQEHDELSFK 450
AGDELTKIED EDEQGWCKGR LDSGQVGLYP ANYVEAIQ 488
Length:488
Mass (Da):55,978
Last modified:August 13, 2002 - v2
Checksum:iB2975012EF0FDF56
GO
Isoform 2 (identifier: Q9QY17-2) [UniParc]FASTAAdd to Basket

Also known as: Ab

The sequence of this isoform differs from the canonical sequence as follows:
     346-386: Missing.

Show »
Length:447
Mass (Da):51,538
Checksum:iB202B81D7D48E6D1
GO
Isoform 3 (identifier: Q9QY17-3) [UniParc]FASTAAdd to Basket

Also known as: Ba

The sequence of this isoform differs from the canonical sequence as follows:
     302-303: Missing.

Show »
Length:486
Mass (Da):55,734
Checksum:iB140F324EF11E29B
GO
Isoform 4 (identifier: Q9QY17-4) [UniParc]FASTAAdd to Basket

Also known as: Bb, SdpII-s

The sequence of this isoform differs from the canonical sequence as follows:
     302-303: Missing.
     346-386: Missing.

Show »
Length:445
Mass (Da):51,294
Checksum:i99512F063951F4A6
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei302 – 3032Missing in isoform 3 and isoform 4.
VSP_004518
Alternative sequencei346 – 38641Missing in isoform 2 and isoform 4.
VSP_004519Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF139492 mRNA. Translation: AAF22211.1.
AF139493 mRNA. Translation: AAF22212.1.
AF139494 mRNA. Translation: AAF22213.1.
AF139495 mRNA. Translation: AAF22214.1.
RefSeqiNP_570096.2. NM_130740.2. [Q9QY17-3]
XP_006242112.1. XM_006242050.1. [Q9QY17-1]
XP_006242113.1. XM_006242051.1. [Q9QY17-1]
XP_006242114.1. XM_006242052.1. [Q9QY17-1]
XP_006242115.1. XM_006242053.1. [Q9QY17-1]
XP_006242117.1. XM_006242055.1. [Q9QY17-3]
XP_006242119.1. XM_006242057.1. [Q9QY17-2]
XP_006242120.1. XM_006242058.1. [Q9QY17-2]
XP_006242121.1. XM_006242059.1. [Q9QY17-4]
XP_006242122.1. XM_006242060.1. [Q9QY17-4]
UniGeneiRn.17106.

Genome annotation databases

EnsembliENSRNOT00000013398; ENSRNOP00000013398; ENSRNOG00000009756. [Q9QY17-3]
GeneIDi124461.
KEGGirno:124461.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF139492 mRNA. Translation: AAF22211.1 .
AF139493 mRNA. Translation: AAF22212.1 .
AF139494 mRNA. Translation: AAF22213.1 .
AF139495 mRNA. Translation: AAF22214.1 .
RefSeqi NP_570096.2. NM_130740.2. [Q9QY17-3 ]
XP_006242112.1. XM_006242050.1. [Q9QY17-1 ]
XP_006242113.1. XM_006242051.1. [Q9QY17-1 ]
XP_006242114.1. XM_006242052.1. [Q9QY17-1 ]
XP_006242115.1. XM_006242053.1. [Q9QY17-1 ]
XP_006242117.1. XM_006242055.1. [Q9QY17-3 ]
XP_006242119.1. XM_006242057.1. [Q9QY17-2 ]
XP_006242120.1. XM_006242058.1. [Q9QY17-2 ]
XP_006242121.1. XM_006242059.1. [Q9QY17-4 ]
XP_006242122.1. XM_006242060.1. [Q9QY17-4 ]
UniGenei Rn.17106.

3D structure databases

ProteinModelPortali Q9QY17.
ModBasei Search...

Protein-protein interaction databases

IntActi Q9QY17. 5 interactions.
MINTi MINT-4567486.
STRINGi 10116.ENSRNOP00000013398.

PTM databases

PhosphoSitei Q9QY17.

Proteomic databases

PaxDbi Q9QY17.
PRIDEi Q9QY17.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000013398 ; ENSRNOP00000013398 ; ENSRNOG00000009756 . [Q9QY17-3 ]
GeneIDi 124461.
KEGGi rno:124461.

Organism-specific databases

CTDi 11252.
RGDi 69411. Pacsin2.

Phylogenomic databases

eggNOGi NOG283356.
GeneTreei ENSGT00510000046376.
HOGENOMi HOG000007245.
HOVERGENi HBG053486.
InParanoidi Q9QY17.
OrthoDBi EOG75TMBJ.
PhylomeDBi Q9QY17.

Miscellaneous databases

NextBioi 620467.
PROi Q9QY17.

Gene expression databases

Genevestigatori Q9QY17.

Family and domain databases

InterProi IPR001060. FCH_dom.
IPR028521. PACSIN2.
IPR001452. SH3_domain.
[Graphical view ]
PANTHERi PTHR10959:SF2. PTHR10959:SF2. 1 hit.
Pfami PF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view ]
PRINTSi PR00452. SH3DOMAIN.
SMARTi SM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
PROSITEi PS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Syndapin isoforms participate in receptor-mediated endocytosis and actin organization."
    Qualmann B., Kelly R.B.
    J. Cell Biol. 148:1047-1062(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3 AND 4), FUNCTION, INTERACTION WITH DNM1; SYN1; SYNJ1 AND WASL, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiPACN2_RAT
AccessioniPrimary (citable) accession number: Q9QY17
Secondary accession number(s): Q9QY18, Q9QY19, Q9QY20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: August 13, 2002
Last modified: June 11, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi