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Protein

ATP-dependent RNA helicase DDX25

Gene

Ddx25

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development (By similarity).By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi143 – 1508ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATPase activity Source: RGD
  • ATP binding Source: UniProtKB
  • ATP-dependent RNA helicase activity Source: UniProtKB
  • RNA binding Source: RGD
  • RNA helicase activity Source: RGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Differentiation, mRNA transport, Spermatogenesis, Translation regulation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BRENDAi3.6.4.12. 5301.
3.6.4.13. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX25 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 25
Gonadotropin-regulated testicular RNA helicase
Gene namesi
Name:Ddx25
Synonyms:Grth
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi68381. Ddx25.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids (By similarity).By similarity

GO - Cellular componenti

  • chromatoid body Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi66 – 661L → A: Enhances nuclear expression; when associated with A-67 and A-70. 1 Publication
Mutagenesisi67 – 671L → A: Enhances nuclear expression; when associated with A-66 and A-70. 1 Publication
Mutagenesisi70 – 701L → A: Enhances nuclear expression; when associated with A-66 and A-67. 1 Publication
Mutagenesisi103 – 1031R → A: Abolishes nuclear expression. 1 Publication
Mutagenesisi104 – 1041L → A: Decreases nuclear expression; when associated with A-108 and A-109. 1 Publication
Mutagenesisi105 – 1051K → A: Abolishes nuclear expression. 1 Publication
Mutagenesisi108 – 1081L → A: Decreases nuclear expression; when associated with A-104 and A-109. 1 Publication
Mutagenesisi109 – 1091L → A: Decreases nuclear expression; when associated with A-104 and A-108. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 483483ATP-dependent RNA helicase DDX25PRO_0000030817Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QY16.
PRIDEiQ9QY16.

Expressioni

Tissue specificityi

Isoform 1 is expressed in germ cells. Isoform 2 is highly expressed in Leydig cells and weakly expressed in the pituitary and hypothalamus. Isoform 3 is weakly expressed only in germ cells.1 Publication

Developmental stagei

Expressed in pubertal and adult animals but not in immature animals.1 Publication

Inductioni

By gonadotropin in Leydig cells. Inhibited by flutamine.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017307.

Structurei

3D structure databases

ProteinModelPortaliQ9QY16.
SMRiQ9QY16. Positions 307-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini130 – 300171Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini311 – 478168Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi61 – 7414Nuclear export signalAdd
BLAST
Motifi97 – 12529Q motifAdd
BLAST
Motifi100 – 11415Nuclear localization signal1 PublicationAdd
BLAST
Motifi247 – 2504DEAD box

Sequence similaritiesi

Belongs to the DEAD box helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ9QY16.
KOiK18656.
PhylomeDBiQ9QY16.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q9QY16-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLLWGGDA GAAESERLNS HFSNLVHPRK NLRGIRSTTV PNIDGSLNTE
60 70 80 90 100
EDDDEDDVVD LAANSLLNKL IRQSLVESSH RVEVLQKDPS SPLYSVKTFE
110 120 130 140 150
ELRLKEELLK GIYAMGFNRP SKIQEMALPM MLAHPPQNLI AQSQSGTGKT
160 170 180 190 200
AAFVLAMLNR VNALELFPQC LCLAPTYELA LQTGRVVERM GKFCVDVEVM
210 220 230 240 250
YAIRGNRIPR GTDVTKQIVI GTPGTVLDWC FKRKLIDLTK IRVFVLDEAD
260 270 280 290 300
VMIDTQGFSD QSIRIQRALP SECQMLLFSA TFEDSVWQFA ERIIPDPNVI
310 320 330 340 350
KLRKEELTLN NIRQYYVLCE NRKDKYQALC NIYGGITIGQ AIIFCQTRRN
360 370 380 390 400
AKWLTVEMMQ DGHQVSLLSG ELTVEQRASI IQRFRDGKEK VLITTNVCAR
410 420 430 440 450
GIDVKQVTIV VNFDLPVNQS EEPDYETYLH RIGRTGRFGK KGLAFNMIEV
460 470 480
DKLPLLMKIQ DHFNSSIKQL DPEDMDEIEK IEY
Length:483
Mass (Da):54,791
Last modified:October 10, 2003 - v2
Checksum:i17F596DAD9E529FC
GO
Isoform 2 (identifier: Q9QY16-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-114: Missing.

Show »
Length:369
Mass (Da):42,134
Checksum:iB9E4E3F63BC2B4E8
GO
Isoform 3 (identifier: Q9QY16-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-189: Missing.

Note: May start at Met-200 rather than Met-190.
Show »
Length:294
Mass (Da):33,923
Checksum:iBC141CC66E7D0613
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 189189Missing in isoform 3. CuratedVSP_018878Add
BLAST
Alternative sequencei1 – 114114Missing in isoform 2. CuratedVSP_018877Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142629 mRNA. Translation: AAF21360.2.
RefSeqiNP_113818.2. NM_031630.2. [Q9QY16-1]
UniGeneiRn.224821.

Genome annotation databases

GeneIDi58856.
KEGGirno:58856.
UCSCiRGD:68381. rat. [Q9QY16-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142629 mRNA. Translation: AAF21360.2.
RefSeqiNP_113818.2. NM_031630.2. [Q9QY16-1]
UniGeneiRn.224821.

3D structure databases

ProteinModelPortaliQ9QY16.
SMRiQ9QY16. Positions 307-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017307.

Proteomic databases

PaxDbiQ9QY16.
PRIDEiQ9QY16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi58856.
KEGGirno:58856.
UCSCiRGD:68381. rat. [Q9QY16-1]

Organism-specific databases

CTDi29118.
RGDi68381. Ddx25.

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ9QY16.
KOiK18656.
PhylomeDBiQ9QY16.

Enzyme and pathway databases

BRENDAi3.6.4.12. 5301.
3.6.4.13. 5301.

Miscellaneous databases

NextBioi611449.
PROiQ9QY16.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel gonadotropin-regulated testicular RNA helicase: a new member of the DEAD-box family."
    Tang P.-Z., Tsai-Morris C.-H., Dufau M.L.
    J. Biol. Chem. 274:37932-37940(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Testis.
  2. "Cell-specific and hormone-regulated expression of gonadotropin-regulated testicular RNA helicase gene (GRTH/Ddx25) resulting from alternative utilization of translation initiation codons in the rat testis."
    Sheng Y., Tsai-Morris C.-H., Dufau M.L.
    J. Biol. Chem. 278:27796-27803(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SEQUENCE REVISION TO N-TERMINUS, ALTERNATIVE INITIATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
  3. "Gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) is a transport protein involved in gene-specific mRNA export and protein translation during spermatogenesis."
    Sheng Y., Tsai-Morris C.-H., Gutti R., Maeda Y., Dufau M.L.
    J. Biol. Chem. 281:35048-35056(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION, NUCLEAR EXPORT SIGNAL, NUCLEAR LOCALIZATION SIGNAL, MUTAGENESIS OF LEU-66; LEU-67; LEU-70; ARG-103; LEU-104; LYS-105; LEU-108 AND LEU-109.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDDX25_RAT
AccessioniPrimary (citable) accession number: Q9QY16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: October 10, 2003
Last modified: May 11, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.