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Protein

ATP-dependent RNA helicase DDX25

Gene

Ddx25

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi144 – 151ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: MGI
  • ATP binding Source: UniProtKB
  • ATP-dependent RNA helicase activity Source: UniProtKB
  • RNA binding Source: MGI
  • RNA helicase activity Source: MGI

GO - Biological processi

  • mRNA export from nucleus Source: UniProtKB
  • multicellular organism development Source: UniProtKB-KW
  • regulation of translation Source: UniProtKB
  • RNA secondary structure unwinding Source: GO_Central
  • RNA splicing Source: GO_Central
  • spermatid development Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Helicase, Hydrolase, RNA-binding
Biological processDifferentiation, mRNA transport, Spermatogenesis, Translation regulation, Transport
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.13 3474

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX25 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 25
Gonadotropin-regulated testicular RNA helicase
Gene namesi
Name:Ddx25
Synonyms:Grth
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1353582 Ddx25

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Male mice display normal sexual behavior but are sterile with testes that are 25% smaller than the wild-type. Round spermatids arrest at step 8 and fail to elongate. Chromatoid bodies are unusually condensed, greatly reduced in size and lack the typical amorphous texture throughout all steps of spermiogenesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000308151 – 484ATP-dependent RNA helicase DDX25Add BLAST484

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei49PhosphothreonineCombined sources1

Post-translational modificationi

Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9QY15
PeptideAtlasiQ9QY15
PRIDEiQ9QY15

PTM databases

iPTMnetiQ9QY15
PhosphoSitePlusiQ9QY15

Expressioni

Tissue specificityi

Isoform 1 is expressed in germ cells. Isoform 2 is expressed in Leydig cells and in round spermatids of adult testis upon gonadotropin stimulation.1 Publication

Gene expression databases

BgeeiENSMUSG00000032101
CleanExiMM_DDX25
GenevisibleiQ9QY15 MM

Interactioni

Protein-protein interaction databases

IntActiQ9QY15, 1 interactor
MINTiQ9QY15
STRINGi10090.ENSMUSP00000034612

Structurei

3D structure databases

ProteinModelPortaliQ9QY15
SMRiQ9QY15
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini131 – 301Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST171
Domaini312 – 479Helicase C-terminalPROSITE-ProRule annotationAdd BLAST168

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi62 – 75Nuclear export signalBy similarityAdd BLAST14
Motifi98 – 126Q motifAdd BLAST29
Motifi101 – 115Nuclear localization signalBy similarityAdd BLAST15
Motifi248 – 251DEAD box4

Sequence similaritiesi

Belongs to the DEAD box helicase family.Curated

Phylogenomic databases

eggNOGiKOG0332 Eukaryota
ENOG410XRGX LUCA
GeneTreeiENSGT00530000063236
HOGENOMiHOG000268797
HOVERGENiHBG107989
InParanoidiQ9QY15
KOiK18656
OMAiCEHRKDK
OrthoDBiEOG091G03PS
PhylomeDBiQ9QY15
TreeFamiTF314957

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR014014 RNA_helicase_DEAD_Q_motif
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51195 Q_MOTIF, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q9QY15-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLLWGGDA GAAESERLNS HFSNLVHPRK NLRGIRSTTV PNIDGSLNTE
60 70 80 90 100
DDDDDEDDVV DLAANSLLNK LIRQSLIESS HRVEVLQKDP SSPLYSVKTF
110 120 130 140 150
EELRLKEELL KGIYAMGFNR PSKIQEMALP MMLAHPPQNL IAQSQSGTGK
160 170 180 190 200
TAAFVLAMLS RVNALELFPQ CLCLAPTYEL ALQTGRVVER MGKFCVDVEV
210 220 230 240 250
MYAIRGNRIP RGTEVTKQII IGTPGTVLDW CFKRKLIDLT KIRVFVLDEA
260 270 280 290 300
DVMIDTQGFS DQSIRIQRAL PSECQMLLFS ATFEDSVWQF AERIIPDPNV
310 320 330 340 350
IKLRKEELTL NNIRQYYVLC ENRKGKYQAL CNIYGGITIG QAIIFCQTRR
360 370 380 390 400
NAKWLTVEMM QDGHQVSLLS GELTVEQRAS IIQRFRDGKE KVLITTNVCA
410 420 430 440 450
RGIDVKQVTI VVNFDLPVNQ SEEPDYETYL HRIGRTGRFG KKGLAFNMIE
460 470 480
VDKLPLLMKI QDHFNSNIKQ LDPEDMDEIE KIEY
Length:484
Mass (Da):54,876
Last modified:October 10, 2003 - v2
Checksum:iD4971BFA338D8BE0
GO
Isoform 2 (identifier: Q9QY15-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-115: Missing.

Show »
Length:369
Mass (Da):42,104
Checksum:i8538CA1D7CE2B3A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti463H → P in AAH24852 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0188761 – 115Missing in isoform 2. CuratedAdd BLAST115

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142630 mRNA Translation: AAF21361.2
AY380091
, AY380080, AY380081, AY380082, AY380083, AY380084, AY380085, AY380086, AY380087, AY380088, AY380089, AY380090 Genomic DNA Translation: AAR26239.1
AK050693 mRNA Translation: BAC34384.1
AK078340 mRNA Translation: BAC37227.1
BC024852 mRNA Translation: AAH24852.1
BC061130 mRNA Translation: AAH61130.2
CCDSiCCDS52753.1 [Q9QY15-1]
RefSeqiNP_038960.2, NM_013932.4 [Q9QY15-1]
UniGeneiMm.291723

Genome annotation databases

EnsembliENSMUST00000034612; ENSMUSP00000034612; ENSMUSG00000032101 [Q9QY15-1]
GeneIDi30959
KEGGimmu:30959
UCSCiuc012gqi.1 mouse [Q9QY15-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry nameiDDX25_MOUSE
AccessioniPrimary (citable) accession number: Q9QY15
Secondary accession number(s): Q53Z03, Q7TMB5, Q8R1B6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: October 10, 2003
Last modified: June 20, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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