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Protein

ATP-dependent RNA helicase DDX25

Gene

Ddx25

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent RNA helicase. Required for mRNA export and translation regulation during spermatid development.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi144 – 1518ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • ATP-dependent RNA helicase activity Source: UniProtKB
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • mRNA export from nucleus Source: UniProtKB
  • multicellular organism development Source: UniProtKB-KW
  • regulation of translation Source: UniProtKB
  • RNA secondary structure unwinding Source: GO_Central
  • spermatid development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Helicase, Hydrolase

Keywords - Biological processi

Differentiation, mRNA transport, Spermatogenesis, Translation regulation, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Enzyme and pathway databases

BRENDAi3.6.4.13. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent RNA helicase DDX25 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 25
Gonadotropin-regulated testicular RNA helicase
Gene namesi
Name:Ddx25
Synonyms:Grth
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1353582. Ddx25.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Detected in both cytoplasm and nucleus of testicular cells. Also detected in chromatoid bodies of round spermatids.

GO - Cellular componenti

  • chromatoid body Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Male mice display normal sexual behavior but are sterile with testes that are 25% smaller than the wild-type. Round spermatids arrest at step 8 and fail to elongate. Chromatoid bodies are unusually condensed, greatly reduced in size and lack the typical amorphous texture throughout all steps of spermiogenesis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 484484ATP-dependent RNA helicase DDX25PRO_0000030815Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei49 – 491PhosphothreonineCombined sources

Post-translational modificationi

Phosphorylated on threonine residues. The phosphorylated form is found in the cytoplasm but not in the nucleus.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9QY15.
PaxDbiQ9QY15.
PeptideAtlasiQ9QY15.
PRIDEiQ9QY15.

PTM databases

iPTMnetiQ9QY15.
PhosphoSiteiQ9QY15.

Expressioni

Tissue specificityi

Isoform 1 is expressed in germ cells. Isoform 2 is expressed in Leydig cells and in round spermatids of adult testis upon gonadotropin stimulation.1 Publication

Gene expression databases

BgeeiENSMUSG00000032101.
CleanExiMM_DDX25.
GenevisibleiQ9QY15. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9QY15. 1 interaction.
MINTiMINT-5201791.
STRINGi10090.ENSMUSP00000034612.

Structurei

3D structure databases

ProteinModelPortaliQ9QY15.
SMRiQ9QY15. Positions 81-477.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini131 – 301171Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini312 – 479168Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi62 – 7514Nuclear export signalBy similarityAdd
BLAST
Motifi98 – 12629Q motifAdd
BLAST
Motifi101 – 11515Nuclear localization signalBy similarityAdd
BLAST
Motifi248 – 2514DEAD box

Sequence similaritiesi

Belongs to the DEAD box helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
GeneTreeiENSGT00530000063236.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ9QY15.
KOiK18656.
OMAiWLTVEMI.
OrthoDBiEOG091G03PS.
PhylomeDBiQ9QY15.
TreeFamiTF314957.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q9QY15-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASLLWGGDA GAAESERLNS HFSNLVHPRK NLRGIRSTTV PNIDGSLNTE
60 70 80 90 100
DDDDDEDDVV DLAANSLLNK LIRQSLIESS HRVEVLQKDP SSPLYSVKTF
110 120 130 140 150
EELRLKEELL KGIYAMGFNR PSKIQEMALP MMLAHPPQNL IAQSQSGTGK
160 170 180 190 200
TAAFVLAMLS RVNALELFPQ CLCLAPTYEL ALQTGRVVER MGKFCVDVEV
210 220 230 240 250
MYAIRGNRIP RGTEVTKQII IGTPGTVLDW CFKRKLIDLT KIRVFVLDEA
260 270 280 290 300
DVMIDTQGFS DQSIRIQRAL PSECQMLLFS ATFEDSVWQF AERIIPDPNV
310 320 330 340 350
IKLRKEELTL NNIRQYYVLC ENRKGKYQAL CNIYGGITIG QAIIFCQTRR
360 370 380 390 400
NAKWLTVEMM QDGHQVSLLS GELTVEQRAS IIQRFRDGKE KVLITTNVCA
410 420 430 440 450
RGIDVKQVTI VVNFDLPVNQ SEEPDYETYL HRIGRTGRFG KKGLAFNMIE
460 470 480
VDKLPLLMKI QDHFNSNIKQ LDPEDMDEIE KIEY
Length:484
Mass (Da):54,876
Last modified:October 10, 2003 - v2
Checksum:iD4971BFA338D8BE0
GO
Isoform 2 (identifier: Q9QY15-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-115: Missing.

Show »
Length:369
Mass (Da):42,104
Checksum:i8538CA1D7CE2B3A7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti463 – 4631H → P in AAH24852 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 115115Missing in isoform 2. CuratedVSP_018876Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142630 mRNA. Translation: AAF21361.2.
AY380091
, AY380080, AY380081, AY380082, AY380083, AY380084, AY380085, AY380086, AY380087, AY380088, AY380089, AY380090 Genomic DNA. Translation: AAR26239.1.
AK050693 mRNA. Translation: BAC34384.1.
AK078340 mRNA. Translation: BAC37227.1.
BC024852 mRNA. Translation: AAH24852.1.
BC061130 mRNA. Translation: AAH61130.2.
CCDSiCCDS52753.1. [Q9QY15-1]
RefSeqiNP_038960.2. NM_013932.4. [Q9QY15-1]
UniGeneiMm.291723.

Genome annotation databases

EnsembliENSMUST00000034612; ENSMUSP00000034612; ENSMUSG00000032101. [Q9QY15-1]
GeneIDi30959.
KEGGimmu:30959.
UCSCiuc012gqi.1. mouse. [Q9QY15-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF142630 mRNA. Translation: AAF21361.2.
AY380091
, AY380080, AY380081, AY380082, AY380083, AY380084, AY380085, AY380086, AY380087, AY380088, AY380089, AY380090 Genomic DNA. Translation: AAR26239.1.
AK050693 mRNA. Translation: BAC34384.1.
AK078340 mRNA. Translation: BAC37227.1.
BC024852 mRNA. Translation: AAH24852.1.
BC061130 mRNA. Translation: AAH61130.2.
CCDSiCCDS52753.1. [Q9QY15-1]
RefSeqiNP_038960.2. NM_013932.4. [Q9QY15-1]
UniGeneiMm.291723.

3D structure databases

ProteinModelPortaliQ9QY15.
SMRiQ9QY15. Positions 81-477.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QY15. 1 interaction.
MINTiMINT-5201791.
STRINGi10090.ENSMUSP00000034612.

PTM databases

iPTMnetiQ9QY15.
PhosphoSiteiQ9QY15.

Proteomic databases

MaxQBiQ9QY15.
PaxDbiQ9QY15.
PeptideAtlasiQ9QY15.
PRIDEiQ9QY15.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034612; ENSMUSP00000034612; ENSMUSG00000032101. [Q9QY15-1]
GeneIDi30959.
KEGGimmu:30959.
UCSCiuc012gqi.1. mouse. [Q9QY15-1]

Organism-specific databases

CTDi29118.
MGIiMGI:1353582. Ddx25.

Phylogenomic databases

eggNOGiKOG0332. Eukaryota.
ENOG410XRGX. LUCA.
GeneTreeiENSGT00530000063236.
HOGENOMiHOG000268797.
HOVERGENiHBG107989.
InParanoidiQ9QY15.
KOiK18656.
OMAiWLTVEMI.
OrthoDBiEOG091G03PS.
PhylomeDBiQ9QY15.
TreeFamiTF314957.

Enzyme and pathway databases

BRENDAi3.6.4.13. 3474.

Miscellaneous databases

PROiQ9QY15.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032101.
CleanExiMM_DDX25.
GenevisibleiQ9QY15. MM.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamiPF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDDX25_MOUSE
AccessioniPrimary (citable) accession number: Q9QY15
Secondary accession number(s): Q53Z03, Q7TMB5, Q8R1B6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: October 10, 2003
Last modified: September 7, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.