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Protein

Unconventional myosin-IXb

Gene

Myo9b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Binds actin with high affinity both in the absence and presence of ATP and its mechanochemical activity is inhibited by calcium ions. Also acts as a GTPase activator for RHOA. Plays a role in the regulation of cell migration via its role as RHOA GTPase activator. This is regulated by its interaction with the SLIT2 receptor ROBO1; interaction with ROBO1 impairs interaction with RHOA and subsequent activation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi239 – 246ATPSequence analysis8
Zinc fingeri1590 – 1639Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST50

GO - Molecular functioni

GO - Biological processi

  • actin filament-based movement Source: UniProtKB
  • actin-myosin filament sliding Source: MGI
  • establishment of cell polarity Source: MGI
  • lamellipodium morphogenesis Source: MGI
  • macrophage chemotaxis Source: MGI
  • monocyte chemotaxis Source: MGI
  • positive regulation of GTPase activity Source: MGI
  • regulation of Rho protein signal transduction Source: UniProtKB
  • Rho protein signal transduction Source: MGI
  • Roundabout signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Motor protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Unconventional myosin-IXb
Alternative name(s):
Unconventional myosin-9b
Gene namesi
Name:Myo9b
Synonyms:Myr5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:106624. Myo9b.

Subcellular locationi

  • Cytoplasmcell cortex By similarity
  • Cytoplasmperinuclear region By similarity
  • Cytoplasmcytoskeleton By similarity

  • Note: In undifferentiated cells colocalizes with F-actin in the cell periphery while in differentiated cells its localization is cytoplasmic with the highest levels in the perinuclear region.By similarity

GO - Cellular componenti

  • actin filament Source: MGI
  • cell cortex Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • filopodium tip Source: MGI
  • lamellipodium Source: MGI
  • membrane Source: MGI
  • myosin complex Source: UniProtKB-KW
  • perinuclear region of cytoplasm Source: UniProtKB
  • ruffle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001234702 – 2114Unconventional myosin-IXbAdd BLAST2113

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei716PhosphoserineBy similarity1
Modified residuei717PhosphoserineBy similarity1
Modified residuei1045PhosphoserineBy similarity1
Modified residuei1106PhosphoserineBy similarity1
Modified residuei1113PhosphoserineBy similarity1
Modified residuei1175PhosphoserineCombined sources1
Modified residuei1218PhosphoserineCombined sources1
Modified residuei1220PhosphoserineBy similarity1
Modified residuei1235PhosphoserineBy similarity1
Modified residuei1241PhosphoserineBy similarity1
Modified residuei1245PhosphoserineBy similarity1
Modified residuei1264PhosphoserineBy similarity1
Modified residuei1266PhosphoserineCombined sources1
Modified residuei1302PhosphoserineBy similarity1
Modified residuei1317PhosphothreonineCombined sources1
Modified residuei1325PhosphoserineCombined sources1
Modified residuei1327PhosphoserineBy similarity1
Modified residuei1335PhosphoserineCombined sources1
Modified residuei1371PhosphoserineBy similarity1
Modified residuei1647PhosphoserineCombined sources1
Modified residuei1884PhosphoserineBy similarity1
Modified residuei1929PhosphoserineBy similarity1
Modified residuei1949PhosphoserineCombined sources1
Modified residuei1956PhosphoserineBy similarity1
Modified residuei1962PhosphothreonineCombined sources1
Modified residuei2007PhosphoserineBy similarity1
Modified residuei2098PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9QY06.
PaxDbiQ9QY06.
PeptideAtlasiQ9QY06.
PRIDEiQ9QY06.

PTM databases

iPTMnetiQ9QY06.
PhosphoSitePlusiQ9QY06.

Expressioni

Tissue specificityi

Expressed in the brain, heart, muscle and inner ear.1 Publication

Gene expression databases

CleanExiMM_MYO9B.

Interactioni

Subunit structurei

Interacts (via IQ domains) with CALM. Interacts with RHOA. Interacts (via Rho-GAP domain) with ROBO1; this inhibits the interaction with RHOA and the stimulation of RHOA GTPase activity, and thereby increases the levels of active RHOA.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000129220.

Structurei

3D structure databases

ProteinModelPortaliQ9QY06.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 114Ras-associatingPROSITE-ProRule annotationAdd BLAST100
Domaini146 – 953Myosin motorAdd BLAST808
Domaini957 – 977IQ 1PROSITE-ProRule annotationAdd BLAST21
Domaini979 – 1000IQ 2PROSITE-ProRule annotationAdd BLAST22
Domaini1001 – 1023IQ 3PROSITE-ProRule annotationAdd BLAST23
Domaini1024 – 1053IQ 4PROSITE-ProRule annotationAdd BLAST30
Domaini1661 – 1846Rho-GAPPROSITE-ProRule annotationAdd BLAST186

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni844 – 855Actin-bindingAdd BLAST12
Regioni940 – 1044Neck or regulatory domainAdd BLAST105
Regioni1045 – 2114TailAdd BLAST1070
Regioni1697 – 1702Interaction with RHOABy similarity6

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1562 – 1592Sequence analysisAdd BLAST31
Coiled coili1839 – 1859Sequence analysisAdd BLAST21
Coiled coili1915 – 1945Sequence analysisAdd BLAST31

Sequence similaritiesi

Contains 4 IQ domains.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 Ras-associating domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri1590 – 1639Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1453. Eukaryota.
KOG4229. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000113707.
HOVERGENiHBG052558.
InParanoidiQ9QY06.
PhylomeDBiQ9QY06.

Family and domain databases

CDDicd00029. C1. 1 hit.
Gene3Di1.10.555.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR028557. MYO9B.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
IPR002219. PE/DAG-bd.
IPR000159. RA_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR13140:SF306. PTHR13140:SF306. 3 hits.
PfamiPF00612. IQ. 4 hits.
PF00063. Myosin_head. 2 hits.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00109. C1. 1 hit.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00314. RA. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50096. IQ. 3 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QY06-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAHEAGSSG RRQQATYHLH IYPQLSSAGS QTSCRVTATK DSTTSDVIQD
60 70 80 90 100
VVASLHLDGS KHYVLVEVKE SGGEEWVLDA SDSPVHRVLL WPRRAQDEHP
110 120 130 140 150
QEDGYYFLLQ ERNADGSIQY LPIQLLAQPT AACRLVERGL LPRPQADFDD
160 170 180 190 200
LCNLPELTEA NLLQNLKLRF MQQKIYTYAG SILVAINPFK FLPIYNPKYV
210 220 230 240 250
KMYENQQLGK LEPHVFALAD VAYYAMLRKH VNQCIVISGE SGSGKTQSTN
260 270 280 290 300
FLIHCLTALS QKGYASGVER TILGAGPVLE AFGNAKTAHN NNSSRFGKFI
310 320 330 340 350
QVNYLENGIV RGAVVEKYLL EKSRLVSQEK DERNYHVFYY LLLGVSEEER
360 370 380 390 400
LEFQLKQPQD YFYLNQHNLN IEDGEDLKHD FERLQQAMEM VGFLPATKKQ
410 420 430 440 450
IFSVLSAILY LGNVTYKKRA TGRDEGLEVG PPEVLDTLSQ LLKVKRETLV
460 470 480 490 500
EVLTKRKTVT VNDKLILPYS LSEAITARDS MAKSLYSALF DWIVLRINHA
510 520 530 540 550
LLNKKDMEEA VSCLSIGVLD IFGFEDFERN SFEQFCINYA NEQLQYYFTQ
560 570 580 590 600
HIFKLEQEEY QGEGISWHNI DYTDNVGCIH LISKKPTGLF YLLDEESNFP
610 620 630 640 650
HATSHTLLAK FKQQHEDNKY FLGTPVLEPA FIIQHFAGRV KYQIKDFREK
660 670 680 690 700
NMDYMRPDIV ALLRGSDSSY VRQLIGMDPV AVFRWAVLRA AIRAMAVLRE
710 720 730 740 750
AGRLRAERAE KAAGISSPAT RSHMEELPRG ASTPSEKLYR DLHNQIIKSL
760 770 780 790 800
KGLPWQGEDP RRLLQSLSLL QKPRTSFLKS KGIKQKQIIP KNLLDSKSLR
810 820 830 840 850
LIISMTLHDR TTKSLLHLHK KKKPPSISAQ FQTSLNKLLE ALGKAEPFFI
860 870 880 890 900
RCIRSNAEKK ELCFDDELVL QQLRYTGMLE TVRIRRSGYS AKYTFQDFTE
910 920 930 940 950
QFQVLLPKDV QPCREAIAAL LEKLQVDRQN YQIGKTKVFL KETERQTLQE
960 970 980 990 1000
KLHGEVLRRI LQLQSWFRMV LERKHFVQMK HAALTIQACW RSYRVRRALE
1010 1020 1030 1040 1050
RTQAAVYLQA AWRGYLQRQA YHHQRHSIIR LQSLCRGHLQ RRSFSQMVSE
1060 1070 1080 1090 1100
KQKAEQAREA AGGKLSEGEP GPVAAGEQLS EHPVEDPESL GVEAETWMNK
1110 1120 1130 1140 1150
SPDGMSPKKE TPSPEMETAA QKTVPAESHE KVSSSREKRE SRRQRGLEHV
1160 1170 1180 1190 1200
ERQNKHIQSC REESSTHREP SRRASLEIGE SFPEGTKGPR EDGLEAWTET
1210 1220 1230 1240 1250
TAPSSSKQAQ VVGDPPGSPS PVQRPTTLAL DSRVSPMLPS SSLESPKDKD
1260 1270 1280 1290 1300
KDESSTKAQD KPESPSGSTQ IQRYQHPDTE RLATAVEIWR GKKLASAVLS
1310 1320 1330 1340 1350
QSLDLSEKHR ATGAALTPTE ERRISFSTSD ISKLSPVKTS AEIDGDFSSK
1360 1370 1380 1390 1400
KPSIHKKKSG DPSAGPDAGL SPGSQGDSKS AFKRLFLHKA KDKKPSLEGV
1410 1420 1430 1440 1450
EETESNGGQA AQETPARKTL DVPSSQQHRH TTGEKPLKGK KNRNRKVGQI
1460 1470 1480 1490 1500
TVSEKWRESV FRKITNANEL KFLDEFLLNK VNDLRSQKTP IESLFIEATE
1510 1520 1530 1540 1550
RFRSNIKTMY SVPNGKIHVG YKDLMENYQI VVSNLAAERG EKDTNLVLNV
1560 1570 1580 1590 1600
FQSLLDEFTR SYNKTDFERA KSKAQKKKRK QERAVQEHNG HVFASYQVNI
1610 1620 1630 1640 1650
PQSCEQCLSY IWLMDKALLC SVCKMTCHKK CVHKIQSYCS YTGRRKSELG
1660 1670 1680 1690 1700
AEPGHFGVCV DSLTSDKASV PIVLEKLLEH VEMHGLYTEG LYRKSGAANR
1710 1720 1730 1740 1750
TRELRQALQT DPAAVKLEDF PIHAITGVLK QWLRELPEPL MTFAQYGDFL
1760 1770 1780 1790 1800
RAVELPEKQE QLSAIYAVLD HLPEANHTSL ERLIFHLVKV ALLEDVNRMS
1810 1820 1830 1840 1850
PGALAIIFAP CLLRCPDNSD PLTSMKDVLK ITTCVEMLIK EQMRKYKMKM
1860 1870 1880 1890 1900
EEINHLEAAE SIAFRRLSLL RQNAPWPLKL GFSSPYEGVR IKSPRTPVVQ
1910 1920 1930 1940 1950
DLELGALSEE AAGGDEDREK EILMERIQSI KEEKEDITYR LPELDPRGSD
1960 1970 1980 1990 2000
EENLDSETSA STESLLEERG VRGAVEGPPA PALPCPISPT LSPLPEAAAP
2010 2020 2030 2040 2050
PRGRPTSFVT VRVKTPRRTP IMPMANIKLP PGLPLHLTSW APALQEAVVP
2060 2070 2080 2090 2100
VKRREPPARR QDQVHSVYIA PGADLPSQST LIALDHDTIL PGTKRRYSDP
2110
PTYCLPPSSG QANG
Length:2,114
Mass (Da):238,834
Last modified:June 20, 2001 - v2
Checksum:iD774D4B1B2788045
GO
Isoform 2 (identifier: Q9QY06-2) [UniParc]FASTAAdd to basket
Also known as: Q

The sequence of this isoform differs from the canonical sequence as follows:
     740-740: R → RCTGLDFSFERSEELDVNAFEDIMAFYESR

Show »
Length:2,143
Mass (Da):242,238
Checksum:i842A3BEEE32F48E7
GO
Isoform 3 (identifier: Q9QY06-3) [UniParc]FASTAAdd to basket
Also known as: C

The sequence of this isoform differs from the canonical sequence as follows:
     1875-1890: Missing.
     1977-2114: GPPAPALPCP...LPPSSGQANG → E

Show »
Length:1,961
Mass (Da):222,444
Checksum:iAEC7450E4B7307BF
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti1243L → LEVSPVLPSSSL in strain: C57BL/6; contains an in-frame 33 bp imperfect duplication. 1
Natural varianti2083A → P.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003363740R → RCTGLDFSFERSEELDVNAF EDIMAFYESR in isoform 2. Curated1
Alternative sequenceiVSP_0033641875 – 1890Missing in isoform 3. CuratedAdd BLAST16
Alternative sequenceiVSP_0033651977 – 2114GPPAP…GQANG → E in isoform 3. CuratedAdd BLAST138

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143687 Genomic DNA. Translation: AAF00122.1.
AF143685 mRNA. Translation: AAF00120.1.
AF143686 mRNA. Translation: AAF00121.1. Frameshift.
AF143683 mRNA. Translation: AAF00118.1.
CCDSiCCDS40379.1. [Q9QY06-3]
UniGeneiMm.33779.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143687 Genomic DNA. Translation: AAF00122.1.
AF143685 mRNA. Translation: AAF00120.1.
AF143686 mRNA. Translation: AAF00121.1. Frameshift.
AF143683 mRNA. Translation: AAF00118.1.
CCDSiCCDS40379.1. [Q9QY06-3]
UniGeneiMm.33779.

3D structure databases

ProteinModelPortaliQ9QY06.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000129220.

PTM databases

iPTMnetiQ9QY06.
PhosphoSitePlusiQ9QY06.

Proteomic databases

EPDiQ9QY06.
PaxDbiQ9QY06.
PeptideAtlasiQ9QY06.
PRIDEiQ9QY06.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:106624. Myo9b.

Phylogenomic databases

eggNOGiKOG1453. Eukaryota.
KOG4229. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000113707.
HOVERGENiHBG052558.
InParanoidiQ9QY06.
PhylomeDBiQ9QY06.

Miscellaneous databases

ChiTaRSiMyo9b. mouse.
PROiQ9QY06.
SOURCEiSearch...

Gene expression databases

CleanExiMM_MYO9B.

Family and domain databases

CDDicd00029. C1. 1 hit.
Gene3Di1.10.555.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR028557. MYO9B.
IPR001609. Myosin_head_motor_dom.
IPR027417. P-loop_NTPase.
IPR002219. PE/DAG-bd.
IPR000159. RA_dom.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PANTHERiPTHR13140:SF306. PTHR13140:SF306. 3 hits.
PfamiPF00612. IQ. 4 hits.
PF00063. Myosin_head. 2 hits.
PF00788. RA. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00109. C1. 1 hit.
SM00015. IQ. 4 hits.
SM00242. MYSc. 1 hit.
SM00314. RA. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF52540. SSF52540. 3 hits.
SSF54236. SSF54236. 1 hit.
PROSITEiPS50096. IQ. 3 hits.
PS51456. MYOSIN_MOTOR. 1 hit.
PS50200. RA. 1 hit.
PS50238. RHOGAP. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYO9B_MOUSE
AccessioniPrimary (citable) accession number: Q9QY06
Secondary accession number(s): Q9QY07, Q9QY08, Q9QY09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: November 30, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents an unconventional myosin. This protein should not be confused with the conventional myosin-9 (MYH9).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.