Q9QXZ9 (OPN4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Melanopsin

Alternative name(s):
Opsin-4

Gene names

Name:	Opn4
Synonyms:	Mop, Mopn

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	521 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal. Ref.1 Ref.2 Ref.5
Subcellular location	Cell membrane; Multi-pass membrane protein. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells By similarity. Ref.2
Tissue specificity	Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. Isoform 2 is about 40 times more abundant than isoform 1 in the retina. Isoform 1 is observed with processes in the outer strata of inner plexiform layer (IPL) close to the inner nuclear layer (INL) or is found to be bistratified with processes located both in the inner (ON) or outer (OFF) layers of the IPL. A second population of isoform 1 is identified in processes which are confined to the inner layer of the IPL near to the ganglion cell layer (GCL). Isoform 2 is involved in processes localized to the outer IPL or is bistratified with processes in both the inner and outer layers of the IPL. Isoform 2 is absent in the processes confined only to the inner layer of the IPL. Ref.1 Ref.2
Disruption phenotype	Mice fail to show a pupillar reflex, photoentrainment of the circadian clock and other non-image forming responses to light. Ref.5
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Ontologies

Keywords
Biological process	Biological rhythms Sensory transduction
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phototransduction Inferred from mutant phenotype Ref.5. Source: UniProtKB protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW regulation of circadian rhythm Inferred from mutant phenotype Ref.5. Source: UniProtKB rhodopsin mediated signaling pathway Inferred from direct assay Ref.2. Source: MGI rhythmic process Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Traceable author statement Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay Ref.2. Source: MGI
Molecular_function	11-cis retinal binding Inferred from sequence or structural similarity. Source: UniProtKB G-protein coupled photoreceptor activity Inferred from direct assay Ref.2. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9QXZ9-1)

Also known as: Opn4L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9QXZ9-2)

Also known as: Opn4S;

The sequence of this isoform differs from the canonical sequence as follows:
     455-521: VQRSKTPKVP...LEDDVTLRHL → TKGHLPSLDLGM

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 521	521	Melanopsin		PRO_0000197816
Regions
	Topological domain	1 – 71	71	Extracellular Potential
	Transmembrane	72 – 92	21	Helical; Name=1; Potential
	Topological domain	93 – 106	14	Cytoplasmic Potential
	Transmembrane	107 – 127	21	Helical; Name=2; Potential
	Topological domain	128 – 143	16	Extracellular Potential
	Transmembrane	144 – 164	21	Helical; Name=3; Potential
	Topological domain	165 – 187	23	Cytoplasmic Potential
	Transmembrane	188 – 208	21	Helical; Name=4; Potential
	Topological domain	209 – 237	29	Extracellular Potential
	Transmembrane	238 – 258	21	Helical; Name=5; Potential
	Topological domain	259 – 293	35	Cytoplasmic Potential
	Transmembrane	294 – 314	21	Helical; Name=6; Potential
	Topological domain	315 – 329	15	Extracellular Potential
	Transmembrane	330 – 350	21	Helical; Name=7; Potential
	Topological domain	351 – 521	171	Cytoplasmic Potential
Amino acid modifications
	Modified residue	337	1	N6-(retinylidene)lysine
	Glycosylation	30	1	N-linked (GlcNAc...) Potential
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Disulfide bond	142 ↔ 220		Potential
Natural variations
	Alternative sequence	455 – 521	67	VQRSK…TLRHL → TKGHLPSLDLGM in isoform 2.		VSP_045928

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Opn4L) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 50FD1CBB05669DA9	FASTA	521	57,231
	10 20 30 40 50 60 MDSPSGPRVL SSLTQDPSFT TSPALQGIWN GTQNVSVRAQ LLSVSPTTSA HQAAAWVPFP 70 80 90 100 110 120 TVDVPDHAHY TLGTVILLVG LTGMLGNLTV IYTFCRNRGL RTPANMFIIN LAVSDFLMSV 130 140 150 160 170 180 TQAPVFFASS LYKKWLFGET GCEFYAFCGA VFGITSMITL TAIAMDRYLV ITRPLATIGR 190 200 210 220 230 240 GSKRRTALVL LGVWLYALAW SLPPFFGWSA YVPEGLLTSC SWDYMTFTPQ VRAYTMLLFC 250 260 270 280 290 300 FVFFLPLLII IFCYIFIFRA IRETGRACEG CGESPLRQRR QWQRLQSEWK MAKVALIVIL 310 320 330 340 350 360 LFVLSWAPYS TVALVAFAGY SHILTPYMSS VPAVIAKASA IHNPIIYAIT HPKYRVAIAQ 370 380 390 400 410 420 HLPCLGVLLG VSGQRSHPSL SYRSTHRSTL SSQSSDLSWI SGRKRQESLG SESEVGWTDT 430 440 450 460 470 480 ETTAAWGAAQ QASGQSFCSQ NLEDGELKAS SSPQVQRSKT PKVPGPSTCR PMKGQGARPS 490 500 510 520 SLRGDQKGRL AVCTGLSECP HPHTSQFPLA FLEDDVTLRH L
	Isoform 2 (Opn4S) [UniParc]. Checksum: F6D363F3D10005CA	FASTA	466	51,220
	10 20 30 40 50 60 MDSPSGPRVL SSLTQDPSFT TSPALQGIWN GTQNVSVRAQ LLSVSPTTSA HQAAAWVPFP 70 80 90 100 110 120 TVDVPDHAHY TLGTVILLVG LTGMLGNLTV IYTFCRNRGL RTPANMFIIN LAVSDFLMSV 130 140 150 160 170 180 TQAPVFFASS LYKKWLFGET GCEFYAFCGA VFGITSMITL TAIAMDRYLV ITRPLATIGR 190 200 210 220 230 240 GSKRRTALVL LGVWLYALAW SLPPFFGWSA YVPEGLLTSC SWDYMTFTPQ VRAYTMLLFC 250 260 270 280 290 300 FVFFLPLLII IFCYIFIFRA IRETGRACEG CGESPLRQRR QWQRLQSEWK MAKVALIVIL 310 320 330 340 350 360 LFVLSWAPYS TVALVAFAGY SHILTPYMSS VPAVIAKASA IHNPIIYAIT HPKYRVAIAQ 370 380 390 400 410 420 HLPCLGVLLG VSGQRSHPSL SYRSTHRSTL SSQSSDLSWI SGRKRQESLG SESEVGWTDT 430 440 450 460 ETTAAWGAAQ QASGQSFCSQ NLEDGELKAS SSPQTKGHLP SLDLGM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel human opsin in the inner retina." Provencio I., Rodriguez I.R., Jiang G., Hayes W.P., Moreira E.F., Rollag M.D. J. Neurosci. 20:600-605(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY. Tissue: Retina.
[2]	"Differential expression of two distinct functional isoforms of melanopsin (Opn4) in the mammalian retina." Pires S.S., Hughes S., Turton M., Melyan Z., Peirson S.N., Zheng L., Kosmaoglou M., Bellingham J., Cheetham M.E., Lucas R.J., Foster R.G., Hankins M.W., Halford S. J. Neurosci. 29:12332-12342(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SYNTHESIS OF 1-15 AND 500-514, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: C3H/He. Tissue: Retina.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]	"Melanopsin and rod-cone photoreceptive systems account for all major accessory visual functions in mice." Hattar S., Lucas R.J., Mrosovsky N., Thompson S., Douglas R.H., Hankins M.W., Lem J., Biel M., Hofmann F., Foster R.G., Yau K.-W. Nature 424:76-81(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF147789 mRNA. Translation: AAF24979.1. EU303117 mRNA. Translation: ACA01962.1. EU303118 mRNA. Translation: ACA01963.1. AC114543 Genomic DNA. No translation available. BC139827 mRNA. Translation: AAI39828.1.
IPI	IPI00135704. IPI00896577.
RefSeq	NP_001122071.1. NM_001128599.1. NP_038915.1. NM_013887.2.
UniGene	Mm.272060.
3D structure databases
ProteinModelPortal	Q9QXZ9.
SMR	Q9QXZ9. Positions 29-364.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000022331.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	Q9QXZ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022331; ENSMUSP00000022331; ENSMUSG00000021799. ENSMUST00000168444; ENSMUSP00000126136; ENSMUSG00000021799.
GeneID	30044.
KEGG	mmu:30044.
Organism-specific databases
CTD	94233.
MGI	MGI:1353425. Opn4.
Phylogenomic databases
eggNOG	NOG255465.
GeneTree	ENSGT00690000101836.
HOGENOM	HOG000264261.
HOVERGEN	HBG007871.
InParanoid	Q9QXZ9.
KO	K04255.
OMA	VLTPYMN.
OrthoDB	EOG418BNM.
Gene expression databases
ArrayExpress	Q9QXZ9.
Bgee	Q9QXZ9.
CleanEx	MM_OPN4.
Genevestigator	Q9QXZ9.
GermOnline	ENSMUSG00000021799. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001760. Opsin. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	307136.
SOURCE	Search...

Entry information

Entry name

OPN4_MOUSE

Accession

Primary (citable) accession number: Q9QXZ9
Secondary accession number(s): A4QPG3, B2C712

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program