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Q9QXZ9

- OPN4_MOUSE

UniProt

Q9QXZ9 - OPN4_MOUSE

Protein

Melanopsin

Gene

Opn4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal.3 Publications

    GO - Molecular functioni

    1. 11-cis retinal binding Source: UniProtKB
    2. G-protein coupled photoreceptor activity Source: UniProtKB

    GO - Biological processi

    1. phototransduction Source: UniProtKB
    2. protein-chromophore linkage Source: UniProtKB-KW
    3. regulation of circadian rhythm Source: UniProtKB
    4. rhodopsin mediated signaling pathway Source: MGI
    5. rhythmic process Source: UniProtKB-KW
    6. visual perception Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer

    Keywords - Biological processi

    Biological rhythms

    Keywords - Ligandi

    Chromophore

    Enzyme and pathway databases

    ReactomeiREACT_207651. G alpha (q) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Melanopsin
    Alternative name(s):
    Opsin-4
    Gene namesi
    Name:Opn4
    Synonyms:Mop, Mopn
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1353425. Opn4.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells.By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB
    2. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice fail to show a pupillar reflex, photoentrainment of the circadian clock and other non-image forming responses to light.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 521521MelanopsinPRO_0000197816Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi30 – 301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi142 ↔ 220PROSITE-ProRule annotation
    Modified residuei337 – 3371N6-(retinylidene)lysine

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ9QXZ9.

    Expressioni

    Tissue specificityi

    Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. Isoform 2 is about 40 times more abundant than isoform 1 in the retina. Isoform 1 is observed with processes in the outer strata of inner plexiform layer (IPL) close to the inner nuclear layer (INL) or is found to be bistratified with processes located both in the inner (ON) or outer (OFF) layers of the IPL. A second population of isoform 1 is identified in processes which are confined to the inner layer of the IPL near to the ganglion cell layer (GCL). Isoform 2 is involved in processes localized to the outer IPL or is bistratified with processes in both the inner and outer layers of the IPL. Isoform 2 is absent in the processes confined only to the inner layer of the IPL.2 Publications

    Gene expression databases

    BgeeiQ9QXZ9.
    CleanExiMM_OPN4.
    GenevestigatoriQ9QXZ9.

    Interactioni

    Protein-protein interaction databases

    IntActiQ9QXZ9. 4 interactions.
    STRINGi10090.ENSMUSP00000022331.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QXZ9.
    SMRiQ9QXZ9. Positions 29-364.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7171ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini93 – 10614CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini128 – 14316ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini165 – 18723CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini209 – 23729ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini259 – 29335CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini315 – 32915ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini351 – 521171CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei72 – 9221Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei107 – 12721Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei144 – 16421Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei188 – 20821Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei238 – 25821Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei294 – 31421Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei330 – 35021Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG255465.
    GeneTreeiENSGT00730000110587.
    HOGENOMiHOG000264261.
    HOVERGENiHBG007871.
    InParanoidiQ9QXZ9.
    KOiK04255.
    OMAiRMAIAQH.
    OrthoDBiEOG7CNZG2.
    PhylomeDBiQ9QXZ9.
    TreeFamiTF324998.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001760. Opsin.
    IPR027430. Retinal_BS.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00238. OPSIN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    PS00238. OPSIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q9QXZ9-1) [UniParc]FASTAAdd to Basket

    Also known as: Opn4L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDSPSGPRVL SSLTQDPSFT TSPALQGIWN GTQNVSVRAQ LLSVSPTTSA    50
    HQAAAWVPFP TVDVPDHAHY TLGTVILLVG LTGMLGNLTV IYTFCRNRGL 100
    RTPANMFIIN LAVSDFLMSV TQAPVFFASS LYKKWLFGET GCEFYAFCGA 150
    VFGITSMITL TAIAMDRYLV ITRPLATIGR GSKRRTALVL LGVWLYALAW 200
    SLPPFFGWSA YVPEGLLTSC SWDYMTFTPQ VRAYTMLLFC FVFFLPLLII 250
    IFCYIFIFRA IRETGRACEG CGESPLRQRR QWQRLQSEWK MAKVALIVIL 300
    LFVLSWAPYS TVALVAFAGY SHILTPYMSS VPAVIAKASA IHNPIIYAIT 350
    HPKYRVAIAQ HLPCLGVLLG VSGQRSHPSL SYRSTHRSTL SSQSSDLSWI 400
    SGRKRQESLG SESEVGWTDT ETTAAWGAAQ QASGQSFCSQ NLEDGELKAS 450
    SSPQVQRSKT PKVPGPSTCR PMKGQGARPS SLRGDQKGRL AVCTGLSECP 500
    HPHTSQFPLA FLEDDVTLRH L 521
    Length:521
    Mass (Da):57,231
    Last modified:May 1, 2000 - v1
    Checksum:i50FD1CBB05669DA9
    GO
    Isoform 2 (identifier: Q9QXZ9-2) [UniParc]FASTAAdd to Basket

    Also known as: Opn4S

    The sequence of this isoform differs from the canonical sequence as follows:
         455-521: VQRSKTPKVP...LEDDVTLRHL → TKGHLPSLDLGM

    Show »
    Length:466
    Mass (Da):51,220
    Checksum:iF6D363F3D10005CA
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei455 – 52167VQRSK…TLRHL → TKGHLPSLDLGM in isoform 2. 1 PublicationVSP_045928Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF147789 mRNA. Translation: AAF24979.1.
    EU303117 mRNA. Translation: ACA01962.1.
    EU303118 mRNA. Translation: ACA01963.1.
    AC114543 Genomic DNA. No translation available.
    BC139827 mRNA. Translation: AAI39828.1.
    CCDSiCCDS26943.1. [Q9QXZ9-1]
    CCDS49446.1. [Q9QXZ9-2]
    RefSeqiNP_001122071.1. NM_001128599.1. [Q9QXZ9-2]
    NP_038915.1. NM_013887.2. [Q9QXZ9-1]
    UniGeneiMm.272060.

    Genome annotation databases

    EnsembliENSMUST00000022331; ENSMUSP00000022331; ENSMUSG00000021799. [Q9QXZ9-1]
    ENSMUST00000168444; ENSMUSP00000126136; ENSMUSG00000021799. [Q9QXZ9-2]
    GeneIDi30044.
    KEGGimmu:30044.
    UCSCiuc007tbh.2. mouse. [Q9QXZ9-1]
    uc011ziw.1. mouse.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF147789 mRNA. Translation: AAF24979.1 .
    EU303117 mRNA. Translation: ACA01962.1 .
    EU303118 mRNA. Translation: ACA01963.1 .
    AC114543 Genomic DNA. No translation available.
    BC139827 mRNA. Translation: AAI39828.1 .
    CCDSi CCDS26943.1. [Q9QXZ9-1 ]
    CCDS49446.1. [Q9QXZ9-2 ]
    RefSeqi NP_001122071.1. NM_001128599.1. [Q9QXZ9-2 ]
    NP_038915.1. NM_013887.2. [Q9QXZ9-1 ]
    UniGenei Mm.272060.

    3D structure databases

    ProteinModelPortali Q9QXZ9.
    SMRi Q9QXZ9. Positions 29-364.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9QXZ9. 4 interactions.
    STRINGi 10090.ENSMUSP00000022331.

    Protein family/group databases

    GPCRDBi Search...

    Proteomic databases

    PRIDEi Q9QXZ9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022331 ; ENSMUSP00000022331 ; ENSMUSG00000021799 . [Q9QXZ9-1 ]
    ENSMUST00000168444 ; ENSMUSP00000126136 ; ENSMUSG00000021799 . [Q9QXZ9-2 ]
    GeneIDi 30044.
    KEGGi mmu:30044.
    UCSCi uc007tbh.2. mouse. [Q9QXZ9-1 ]
    uc011ziw.1. mouse.

    Organism-specific databases

    CTDi 94233.
    MGIi MGI:1353425. Opn4.

    Phylogenomic databases

    eggNOGi NOG255465.
    GeneTreei ENSGT00730000110587.
    HOGENOMi HOG000264261.
    HOVERGENi HBG007871.
    InParanoidi Q9QXZ9.
    KOi K04255.
    OMAi RMAIAQH.
    OrthoDBi EOG7CNZG2.
    PhylomeDBi Q9QXZ9.
    TreeFami TF324998.

    Enzyme and pathway databases

    Reactomei REACT_207651. G alpha (q) signalling events.

    Miscellaneous databases

    NextBioi 307136.
    PROi Q9QXZ9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9QXZ9.
    CleanExi MM_OPN4.
    Genevestigatori Q9QXZ9.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001760. Opsin.
    IPR027430. Retinal_BS.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00238. OPSIN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    PS00238. OPSIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel human opsin in the inner retina."
      Provencio I., Rodriguez I.R., Jiang G., Hayes W.P., Moreira E.F., Rollag M.D.
      J. Neurosci. 20:600-605(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Tissue: Retina.
    2. "Differential expression of two distinct functional isoforms of melanopsin (Opn4) in the mammalian retina."
      Pires S.S., Hughes S., Turton M., Melyan Z., Peirson S.N., Zheng L., Kosmaoglou M., Bellingham J., Cheetham M.E., Lucas R.J., Foster R.G., Hankins M.W., Halford S.
      J. Neurosci. 29:12332-12342(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SYNTHESIS OF 1-15 AND 500-514, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: C3H/He.
      Tissue: Retina.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    5. "Melanopsin and rod-cone photoreceptive systems account for all major accessory visual functions in mice."
      Hattar S., Lucas R.J., Mrosovsky N., Thompson S., Douglas R.H., Hankins M.W., Lem J., Biel M., Hofmann F., Foster R.G., Yau K.-W.
      Nature 424:76-81(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiOPN4_MOUSE
    AccessioniPrimary (citable) accession number: Q9QXZ9
    Secondary accession number(s): A4QPG3, B2C712
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3