Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9QXZ9

- OPN4_MOUSE

UniProt

Q9QXZ9 - OPN4_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Melanopsin

Gene

Opn4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal.3 Publications

GO - Molecular functioni

  1. 11-cis retinal binding Source: UniProtKB
  2. G-protein coupled photoreceptor activity Source: UniProtKB

GO - Biological processi

  1. phototransduction Source: UniProtKB
  2. protein-chromophore linkage Source: UniProtKB-KW
  3. regulation of circadian rhythm Source: UniProtKB
  4. rhodopsin mediated signaling pathway Source: MGI
  5. rhythmic process Source: UniProtKB-KW
  6. visual perception Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer

Keywords - Biological processi

Biological rhythms

Keywords - Ligandi

Chromophore

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Melanopsin
Alternative name(s):
Opsin-4
Gene namesi
Name:Opn4
Synonyms:Mop, Mopn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:1353425. Opn4.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells.By similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB
  2. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice fail to show a pupillar reflex, photoentrainment of the circadian clock and other non-image forming responses to light.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 521521MelanopsinPRO_0000197816Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi30 – 301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi34 – 341N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi142 ↔ 220PROSITE-ProRule annotation
Modified residuei337 – 3371N6-(retinylidene)lysine

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ9QXZ9.

Expressioni

Tissue specificityi

Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. Isoform 2 is about 40 times more abundant than isoform 1 in the retina. Isoform 1 is observed with processes in the outer strata of inner plexiform layer (IPL) close to the inner nuclear layer (INL) or is found to be bistratified with processes located both in the inner (ON) or outer (OFF) layers of the IPL. A second population of isoform 1 is identified in processes which are confined to the inner layer of the IPL near to the ganglion cell layer (GCL). Isoform 2 is involved in processes localized to the outer IPL or is bistratified with processes in both the inner and outer layers of the IPL. Isoform 2 is absent in the processes confined only to the inner layer of the IPL.2 Publications

Gene expression databases

BgeeiQ9QXZ9.
CleanExiMM_OPN4.
GenevestigatoriQ9QXZ9.

Interactioni

Protein-protein interaction databases

IntActiQ9QXZ9. 4 interactions.
STRINGi10090.ENSMUSP00000022331.

Structurei

3D structure databases

ProteinModelPortaliQ9QXZ9.
SMRiQ9QXZ9. Positions 29-364.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7171ExtracellularSequence AnalysisAdd
BLAST
Topological domaini93 – 10614CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini128 – 14316ExtracellularSequence AnalysisAdd
BLAST
Topological domaini165 – 18723CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini209 – 23729ExtracellularSequence AnalysisAdd
BLAST
Topological domaini259 – 29335CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini315 – 32915ExtracellularSequence AnalysisAdd
BLAST
Topological domaini351 – 521171CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei72 – 9221Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei107 – 12721Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei144 – 16421Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei188 – 20821Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei238 – 25821Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei294 – 31421Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei330 – 35021Helical; Name=7Sequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG255465.
GeneTreeiENSGT00760000118977.
HOGENOMiHOG000264261.
HOVERGENiHBG007871.
InParanoidiQ9QXZ9.
KOiK04255.
OMAiRMAIAQH.
OrthoDBiEOG7CNZG2.
PhylomeDBiQ9QXZ9.
TreeFamiTF324998.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR027430. Retinal_BS.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9QXZ9-1) [UniParc]FASTAAdd to Basket

Also known as: Opn4L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSPSGPRVL SSLTQDPSFT TSPALQGIWN GTQNVSVRAQ LLSVSPTTSA
60 70 80 90 100
HQAAAWVPFP TVDVPDHAHY TLGTVILLVG LTGMLGNLTV IYTFCRNRGL
110 120 130 140 150
RTPANMFIIN LAVSDFLMSV TQAPVFFASS LYKKWLFGET GCEFYAFCGA
160 170 180 190 200
VFGITSMITL TAIAMDRYLV ITRPLATIGR GSKRRTALVL LGVWLYALAW
210 220 230 240 250
SLPPFFGWSA YVPEGLLTSC SWDYMTFTPQ VRAYTMLLFC FVFFLPLLII
260 270 280 290 300
IFCYIFIFRA IRETGRACEG CGESPLRQRR QWQRLQSEWK MAKVALIVIL
310 320 330 340 350
LFVLSWAPYS TVALVAFAGY SHILTPYMSS VPAVIAKASA IHNPIIYAIT
360 370 380 390 400
HPKYRVAIAQ HLPCLGVLLG VSGQRSHPSL SYRSTHRSTL SSQSSDLSWI
410 420 430 440 450
SGRKRQESLG SESEVGWTDT ETTAAWGAAQ QASGQSFCSQ NLEDGELKAS
460 470 480 490 500
SSPQVQRSKT PKVPGPSTCR PMKGQGARPS SLRGDQKGRL AVCTGLSECP
510 520
HPHTSQFPLA FLEDDVTLRH L
Length:521
Mass (Da):57,231
Last modified:May 1, 2000 - v1
Checksum:i50FD1CBB05669DA9
GO
Isoform 2 (identifier: Q9QXZ9-2) [UniParc]FASTAAdd to Basket

Also known as: Opn4S

The sequence of this isoform differs from the canonical sequence as follows:
     455-521: VQRSKTPKVP...LEDDVTLRHL → TKGHLPSLDLGM

Show »
Length:466
Mass (Da):51,220
Checksum:iF6D363F3D10005CA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei455 – 52167VQRSK…TLRHL → TKGHLPSLDLGM in isoform 2. 1 PublicationVSP_045928Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF147789 mRNA. Translation: AAF24979.1.
EU303117 mRNA. Translation: ACA01962.1.
EU303118 mRNA. Translation: ACA01963.1.
AC114543 Genomic DNA. No translation available.
BC139827 mRNA. Translation: AAI39828.1.
CCDSiCCDS26943.1. [Q9QXZ9-1]
CCDS49446.1. [Q9QXZ9-2]
RefSeqiNP_001122071.1. NM_001128599.1. [Q9QXZ9-2]
NP_038915.1. NM_013887.2. [Q9QXZ9-1]
UniGeneiMm.272060.

Genome annotation databases

EnsembliENSMUST00000022331; ENSMUSP00000022331; ENSMUSG00000021799. [Q9QXZ9-1]
ENSMUST00000168444; ENSMUSP00000126136; ENSMUSG00000021799. [Q9QXZ9-2]
GeneIDi30044.
KEGGimmu:30044.
UCSCiuc007tbh.2. mouse. [Q9QXZ9-1]
uc011ziw.1. mouse.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF147789 mRNA. Translation: AAF24979.1 .
EU303117 mRNA. Translation: ACA01962.1 .
EU303118 mRNA. Translation: ACA01963.1 .
AC114543 Genomic DNA. No translation available.
BC139827 mRNA. Translation: AAI39828.1 .
CCDSi CCDS26943.1. [Q9QXZ9-1 ]
CCDS49446.1. [Q9QXZ9-2 ]
RefSeqi NP_001122071.1. NM_001128599.1. [Q9QXZ9-2 ]
NP_038915.1. NM_013887.2. [Q9QXZ9-1 ]
UniGenei Mm.272060.

3D structure databases

ProteinModelPortali Q9QXZ9.
SMRi Q9QXZ9. Positions 29-364.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9QXZ9. 4 interactions.
STRINGi 10090.ENSMUSP00000022331.

Protein family/group databases

GPCRDBi Search...

Proteomic databases

PRIDEi Q9QXZ9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022331 ; ENSMUSP00000022331 ; ENSMUSG00000021799 . [Q9QXZ9-1 ]
ENSMUST00000168444 ; ENSMUSP00000126136 ; ENSMUSG00000021799 . [Q9QXZ9-2 ]
GeneIDi 30044.
KEGGi mmu:30044.
UCSCi uc007tbh.2. mouse. [Q9QXZ9-1 ]
uc011ziw.1. mouse.

Organism-specific databases

CTDi 94233.
MGIi MGI:1353425. Opn4.

Phylogenomic databases

eggNOGi NOG255465.
GeneTreei ENSGT00760000118977.
HOGENOMi HOG000264261.
HOVERGENi HBG007871.
InParanoidi Q9QXZ9.
KOi K04255.
OMAi RMAIAQH.
OrthoDBi EOG7CNZG2.
PhylomeDBi Q9QXZ9.
TreeFami TF324998.

Enzyme and pathway databases

Reactomei REACT_207651. G alpha (q) signalling events.

Miscellaneous databases

NextBioi 307136.
PROi Q9QXZ9.
SOURCEi Search...

Gene expression databases

Bgeei Q9QXZ9.
CleanExi MM_OPN4.
Genevestigatori Q9QXZ9.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR027430. Retinal_BS.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel human opsin in the inner retina."
    Provencio I., Rodriguez I.R., Jiang G., Hayes W.P., Moreira E.F., Rollag M.D.
    J. Neurosci. 20:600-605(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
    Tissue: Retina.
  2. "Differential expression of two distinct functional isoforms of melanopsin (Opn4) in the mammalian retina."
    Pires S.S., Hughes S., Turton M., Melyan Z., Peirson S.N., Zheng L., Kosmaoglou M., Bellingham J., Cheetham M.E., Lucas R.J., Foster R.G., Hankins M.W., Halford S.
    J. Neurosci. 29:12332-12342(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SYNTHESIS OF 1-15 AND 500-514, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: C3H/He.
    Tissue: Retina.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  5. "Melanopsin and rod-cone photoreceptive systems account for all major accessory visual functions in mice."
    Hattar S., Lucas R.J., Mrosovsky N., Thompson S., Douglas R.H., Hankins M.W., Lem J., Biel M., Hofmann F., Foster R.G., Yau K.-W.
    Nature 424:76-81(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiOPN4_MOUSE
AccessioniPrimary (citable) accession number: Q9QXZ9
Secondary accession number(s): A4QPG3, B2C712
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3