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Protein

Melanopsin

Gene

Opn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal.3 Publications

GO - Molecular functioni

  • 11-cis retinal binding Source: UniProtKB
  • G-protein coupled photoreceptor activity Source: MGI

GO - Biological processi

  • entrainment of circadian clock by photoperiod Source: MGI
  • phototransduction Source: UniProtKB
  • positive regulation of circadian sleep/wake cycle, sleep Source: MGI
  • protein-chromophore linkage Source: UniProtKB-KW
  • regulation of circadian rhythm Source: UniProtKB
  • rhodopsin mediated signaling pathway Source: MGI
  • rhythmic process Source: UniProtKB-KW
  • visual perception Source: InterPro

Keywordsi

Molecular functionG-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer
Biological processBiological rhythms
LigandChromophore

Enzyme and pathway databases

ReactomeiR-MMU-416476 G alpha (q) signalling events
R-MMU-419771 Opsins

Names & Taxonomyi

Protein namesi
Recommended name:
Melanopsin
Alternative name(s):
Opsin-4
Gene namesi
Name:Opn4
Synonyms:Mop, Mopn
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1353425 Opn4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 71ExtracellularSequence analysisAdd BLAST71
Transmembranei72 – 92Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini93 – 106CytoplasmicSequence analysisAdd BLAST14
Transmembranei107 – 127Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini128 – 143ExtracellularSequence analysisAdd BLAST16
Transmembranei144 – 164Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini165 – 187CytoplasmicSequence analysisAdd BLAST23
Transmembranei188 – 208Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini209 – 237ExtracellularSequence analysisAdd BLAST29
Transmembranei238 – 258Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini259 – 293CytoplasmicSequence analysisAdd BLAST35
Transmembranei294 – 314Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini315 – 329ExtracellularSequence analysisAdd BLAST15
Transmembranei330 – 350Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini351 – 521CytoplasmicSequence analysisAdd BLAST171

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice fail to show a pupillar reflex, photoentrainment of the circadian clock and other non-image forming responses to light.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001978161 – 521MelanopsinAdd BLAST521

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi30N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi34N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi142 ↔ 220PROSITE-ProRule annotation
Modified residuei337N6-(retinylidene)lysine1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9QXZ9
PRIDEiQ9QXZ9

PTM databases

iPTMnetiQ9QXZ9
PhosphoSitePlusiQ9QXZ9

Expressioni

Tissue specificityi

Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. Isoform 2 is about 40 times more abundant than isoform 1 in the retina. Isoform 1 is observed with processes in the outer strata of inner plexiform layer (IPL) close to the inner nuclear layer (INL) or is found to be bistratified with processes located both in the inner (ON) or outer (OFF) layers of the IPL. A second population of isoform 1 is identified in processes which are confined to the inner layer of the IPL near to the ganglion cell layer (GCL). Isoform 2 is involved in processes localized to the outer IPL or is bistratified with processes in both the inner and outer layers of the IPL. Isoform 2 is absent in the processes confined only to the inner layer of the IPL.2 Publications

Gene expression databases

BgeeiENSMUSG00000021799
CleanExiMM_OPN4
GenevisibleiQ9QXZ9 MM

Interactioni

Protein-protein interaction databases

IntActiQ9QXZ9, 4 interactors
STRINGi10090.ENSMUSP00000022331

Structurei

3D structure databases

ProteinModelPortaliQ9QXZ9
SMRiQ9QXZ9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656 Eukaryota
ENOG410XRW9 LUCA
GeneTreeiENSGT00760000118977
HOGENOMiHOG000264261
HOVERGENiHBG007871
InParanoidiQ9QXZ9
KOiK04255
OMAiHPKYRMA
OrthoDBiEOG091G0ALC
PhylomeDBiQ9QXZ9
TreeFamiTF324998

Family and domain databases

InterProiView protein in InterPro
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
IPR001760 Opsin
IPR027430 Retinal_BS
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00237 GPCRRHODOPSN
PR00238 OPSIN
SMARTiView protein in SMART
SM01381 7TM_GPCR_Srsx, 1 hit
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit
PS00238 OPSIN, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QXZ9-1) [UniParc]FASTAAdd to basket
Also known as: Opn4L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDSPSGPRVL SSLTQDPSFT TSPALQGIWN GTQNVSVRAQ LLSVSPTTSA
60 70 80 90 100
HQAAAWVPFP TVDVPDHAHY TLGTVILLVG LTGMLGNLTV IYTFCRNRGL
110 120 130 140 150
RTPANMFIIN LAVSDFLMSV TQAPVFFASS LYKKWLFGET GCEFYAFCGA
160 170 180 190 200
VFGITSMITL TAIAMDRYLV ITRPLATIGR GSKRRTALVL LGVWLYALAW
210 220 230 240 250
SLPPFFGWSA YVPEGLLTSC SWDYMTFTPQ VRAYTMLLFC FVFFLPLLII
260 270 280 290 300
IFCYIFIFRA IRETGRACEG CGESPLRQRR QWQRLQSEWK MAKVALIVIL
310 320 330 340 350
LFVLSWAPYS TVALVAFAGY SHILTPYMSS VPAVIAKASA IHNPIIYAIT
360 370 380 390 400
HPKYRVAIAQ HLPCLGVLLG VSGQRSHPSL SYRSTHRSTL SSQSSDLSWI
410 420 430 440 450
SGRKRQESLG SESEVGWTDT ETTAAWGAAQ QASGQSFCSQ NLEDGELKAS
460 470 480 490 500
SSPQVQRSKT PKVPGPSTCR PMKGQGARPS SLRGDQKGRL AVCTGLSECP
510 520
HPHTSQFPLA FLEDDVTLRH L
Length:521
Mass (Da):57,231
Last modified:May 1, 2000 - v1
Checksum:i50FD1CBB05669DA9
GO
Isoform 2 (identifier: Q9QXZ9-2) [UniParc]FASTAAdd to basket
Also known as: Opn4S

The sequence of this isoform differs from the canonical sequence as follows:
     455-521: VQRSKTPKVP...LEDDVTLRHL → TKGHLPSLDLGM

Show »
Length:466
Mass (Da):51,220
Checksum:iF6D363F3D10005CA
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_045928455 – 521VQRSK…TLRHL → TKGHLPSLDLGM in isoform 2. 1 PublicationAdd BLAST67

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF147789 mRNA Translation: AAF24979.1
EU303117 mRNA Translation: ACA01962.1
EU303118 mRNA Translation: ACA01963.1
AC114543 Genomic DNA No translation available.
BC139827 mRNA Translation: AAI39828.1
CCDSiCCDS26943.1 [Q9QXZ9-1]
CCDS49446.1 [Q9QXZ9-2]
RefSeqiNP_001122071.1, NM_001128599.1 [Q9QXZ9-2]
NP_038915.1, NM_013887.2 [Q9QXZ9-1]
UniGeneiMm.272060

Genome annotation databases

EnsembliENSMUST00000022331; ENSMUSP00000022331; ENSMUSG00000021799 [Q9QXZ9-1]
ENSMUST00000168444; ENSMUSP00000126136; ENSMUSG00000021799 [Q9QXZ9-2]
GeneIDi30044
KEGGimmu:30044
UCSCiuc007tbh.2 mouse [Q9QXZ9-1]
uc011ziw.1 mouse [Q9QXZ9-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiOPN4_MOUSE
AccessioniPrimary (citable) accession number: Q9QXZ9
Secondary accession number(s): A4QPG3, B2C712
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: June 20, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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