Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9QXZ9 (OPN4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Melanopsin
Alternative name(s):
Opsin-4
Gene names
Name:Opn4
Synonyms:Mop, Mopn
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Photoreceptor required for regulation of circadian rhythm. Contributes to pupillar reflex and other non-image forming responses to light. May be able to isomerize covalently bound all-trans retinal back to 11-cis retinal. Ref.1 Ref.2 Ref.5

Subcellular location

Cell membrane; Multi-pass membrane protein. Note: Found in soma, dendrites and proximal part of axons of certain retinal ganglion cells By similarity. Ref.2

Tissue specificity

Eye. Expression is restricted within the ganglion and amacrine cell layers of the retina. Isoform 2 is about 40 times more abundant than isoform 1 in the retina. Isoform 1 is observed with processes in the outer strata of inner plexiform layer (IPL) close to the inner nuclear layer (INL) or is found to be bistratified with processes located both in the inner (ON) or outer (OFF) layers of the IPL. A second population of isoform 1 is identified in processes which are confined to the inner layer of the IPL near to the ganglion cell layer (GCL). Isoform 2 is involved in processes localized to the outer IPL or is bistratified with processes in both the inner and outer layers of the IPL. Isoform 2 is absent in the processes confined only to the inner layer of the IPL. Ref.1 Ref.2

Disruption phenotype

Mice fail to show a pupillar reflex, photoentrainment of the circadian clock and other non-image forming responses to light. Ref.5

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9QXZ9-1)

Also known as: Opn4L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9QXZ9-2)

Also known as: Opn4S;

The sequence of this isoform differs from the canonical sequence as follows:
     455-521: VQRSKTPKVP...LEDDVTLRHL → TKGHLPSLDLGM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 521521Melanopsin
PRO_0000197816

Regions

Topological domain1 – 7171Extracellular Potential
Transmembrane72 – 9221Helical; Name=1; Potential
Topological domain93 – 10614Cytoplasmic Potential
Transmembrane107 – 12721Helical; Name=2; Potential
Topological domain128 – 14316Extracellular Potential
Transmembrane144 – 16421Helical; Name=3; Potential
Topological domain165 – 18723Cytoplasmic Potential
Transmembrane188 – 20821Helical; Name=4; Potential
Topological domain209 – 23729Extracellular Potential
Transmembrane238 – 25821Helical; Name=5; Potential
Topological domain259 – 29335Cytoplasmic Potential
Transmembrane294 – 31421Helical; Name=6; Potential
Topological domain315 – 32915Extracellular Potential
Transmembrane330 – 35021Helical; Name=7; Potential
Topological domain351 – 521171Cytoplasmic Potential

Amino acid modifications

Modified residue3371N6-(retinylidene)lysine
Glycosylation301N-linked (GlcNAc...) Potential
Glycosylation341N-linked (GlcNAc...) Potential
Disulfide bond142 ↔ 220 Potential

Natural variations

Alternative sequence455 – 52167VQRSK…TLRHL → TKGHLPSLDLGM in isoform 2.
VSP_045928

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Opn4L) [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 50FD1CBB05669DA9

FASTA52157,231
        10         20         30         40         50         60 
MDSPSGPRVL SSLTQDPSFT TSPALQGIWN GTQNVSVRAQ LLSVSPTTSA HQAAAWVPFP 

        70         80         90        100        110        120 
TVDVPDHAHY TLGTVILLVG LTGMLGNLTV IYTFCRNRGL RTPANMFIIN LAVSDFLMSV 

       130        140        150        160        170        180 
TQAPVFFASS LYKKWLFGET GCEFYAFCGA VFGITSMITL TAIAMDRYLV ITRPLATIGR 

       190        200        210        220        230        240 
GSKRRTALVL LGVWLYALAW SLPPFFGWSA YVPEGLLTSC SWDYMTFTPQ VRAYTMLLFC 

       250        260        270        280        290        300 
FVFFLPLLII IFCYIFIFRA IRETGRACEG CGESPLRQRR QWQRLQSEWK MAKVALIVIL 

       310        320        330        340        350        360 
LFVLSWAPYS TVALVAFAGY SHILTPYMSS VPAVIAKASA IHNPIIYAIT HPKYRVAIAQ 

       370        380        390        400        410        420 
HLPCLGVLLG VSGQRSHPSL SYRSTHRSTL SSQSSDLSWI SGRKRQESLG SESEVGWTDT 

       430        440        450        460        470        480 
ETTAAWGAAQ QASGQSFCSQ NLEDGELKAS SSPQVQRSKT PKVPGPSTCR PMKGQGARPS 

       490        500        510        520 
SLRGDQKGRL AVCTGLSECP HPHTSQFPLA FLEDDVTLRH L 

« Hide

Isoform 2 (Opn4S) [UniParc].

Checksum: F6D363F3D10005CA
Show »

FASTA46651,220

References

« Hide 'large scale' references
[1]"A novel human opsin in the inner retina."
Provencio I., Rodriguez I.R., Jiang G., Hayes W.P., Moreira E.F., Rollag M.D.
J. Neurosci. 20:600-605(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
Tissue: Retina.
[2]"Differential expression of two distinct functional isoforms of melanopsin (Opn4) in the mammalian retina."
Pires S.S., Hughes S., Turton M., Melyan Z., Peirson S.N., Zheng L., Kosmaoglou M., Bellingham J., Cheetham M.E., Lucas R.J., Foster R.G., Hankins M.W., Halford S.
J. Neurosci. 29:12332-12342(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SYNTHESIS OF 1-15 AND 500-514, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: C3H/He.
Tissue: Retina.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[5]"Melanopsin and rod-cone photoreceptive systems account for all major accessory visual functions in mice."
Hattar S., Lucas R.J., Mrosovsky N., Thompson S., Douglas R.H., Hankins M.W., Lem J., Biel M., Hofmann F., Foster R.G., Yau K.-W.
Nature 424:76-81(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF147789 mRNA. Translation: AAF24979.1.
EU303117 mRNA. Translation: ACA01962.1.
EU303118 mRNA. Translation: ACA01963.1.
AC114543 Genomic DNA. No translation available.
BC139827 mRNA. Translation: AAI39828.1.
RefSeqNP_001122071.1. NM_001128599.1.
NP_038915.1. NM_013887.2.
UniGeneMm.272060.

3D structure databases

ProteinModelPortalQ9QXZ9.
SMRQ9QXZ9. Positions 29-364.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ9QXZ9. 4 interactions.
STRING10090.ENSMUSP00000022331.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PRIDEQ9QXZ9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022331; ENSMUSP00000022331; ENSMUSG00000021799. [Q9QXZ9-1]
ENSMUST00000168444; ENSMUSP00000126136; ENSMUSG00000021799. [Q9QXZ9-2]
GeneID30044.
KEGGmmu:30044.
UCSCuc007tbh.2. mouse. [Q9QXZ9-1]
uc011ziw.1. mouse.

Organism-specific databases

CTD94233.
MGIMGI:1353425. Opn4.

Phylogenomic databases

eggNOGNOG255465.
GeneTreeENSGT00730000110587.
HOGENOMHOG000264261.
HOVERGENHBG007871.
InParanoidQ9QXZ9.
KOK04255.
OMARMAIAQH.
OrthoDBEOG7CNZG2.
PhylomeDBQ9QXZ9.
TreeFamTF324998.

Gene expression databases

BgeeQ9QXZ9.
CleanExMM_OPN4.
GenevestigatorQ9QXZ9.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR027430. Retinal_BS.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio307136.
PROQ9QXZ9.
SOURCESearch...

Entry information

Entry nameOPN4_MOUSE
AccessionPrimary (citable) accession number: Q9QXZ9
Secondary accession number(s): A4QPG3, B2C712
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries