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Q9QXZ7

- NR2E3_MOUSE

UniProt

Q9QXZ7 - NR2E3_MOUSE

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Protein

Photoreceptor-specific nuclear receptor

Gene

Nr2e3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Orphan nuclear receptor of retinal photoreceptor cells. Transcriptional factor that is an activator of rod development and repressor of cone development. Binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. Enhances rhodopsin expression. Represses M- and S-cone opsin expression.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi37 – 11377Nuclear receptorPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri40 – 6021NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri76 – 10126NR C4-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: InterPro
  2. sequence-specific DNA binding Source: InterPro
  3. sequence-specific DNA binding transcription factor activity Source: MGI
  4. steroid hormone receptor activity Source: InterPro
  5. zinc ion binding Source: InterPro

GO - Biological processi

  1. eye photoreceptor cell development Source: MGI
  2. negative regulation of cell proliferation Source: MGI
  3. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  4. positive regulation of rhodopsin gene expression Source: MGI
  5. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  6. retina development in camera-type eye Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Photoreceptor-specific nuclear receptor
Alternative name(s):
Nuclear receptor subfamily 2 group E member 3
Retina-specific nuclear receptor
Gene namesi
Name:Nr2e3
Synonyms:Pnr, Rnr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:1346317. Nr2e3.

Subcellular locationi

Nucleus 2 PublicationsPROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: MGI
  2. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Defects in Nr2e3 are the cause of the retinal degeneration type 7 (Rd7) phenotype characterized by excessive blue cones and loss of rods.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi178 – 1781K → R: Greatly reduced levels of PIAS3-mediated sumoylation. Completely abolishes sumoylation; when associated with R-315 and R-322. 1 Publication
Mutagenesisi315 – 3151K → R: Little change in sumoylation. Completely abolishes sumoylation; when associated with R-178 and R-322. 1 Publication
Mutagenesisi322 – 3221K → R: Little change in sumoylation. Completely abolishes sumoylation; when associated with R-178 and R-315. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 395395Photoreceptor-specific nuclear receptorPRO_0000053600Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki178 – 178Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki315 – 315Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Modified residuei318 – 3181PhosphothreonineBy similarity
Cross-linki322 – 322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modificationi

Di- and tri-sumoylated in developing retina. PIAS3-mediated sumoylation promotes repression of cone-specific gene expression and activation of rod-specific genes. Sumoylation on Lys-178 appears to be the main site.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PRIDEiQ9QXZ7.

PTM databases

PhosphoSiteiQ9QXZ7.

Expressioni

Tissue specificityi

Retina. Rod-specific. Expressed in the outer nuclear lyer of the mature retina.5 Publications

Developmental stagei

Expression found as early as E18 in developing retina. From P3 to P6, expression increases in developing rods. Expression, thereafter, in the future inner nuclear layer migrating to the final destination of the outer nuclear layer. In the mature retina, exclusively expressed in rods.1 Publication

Gene expression databases

BgeeiQ9QXZ7.
CleanExiMM_NR2E3.
ExpressionAtlasiQ9QXZ7. differential.
GenevestigatoriQ9QXZ7.

Interactioni

Subunit structurei

Homodimer. Interacts with PIAS3; the interaction sumoylates NR2E3 and promotes repression of cone-specific gene transcription and activation of rod-specific genes. Component of a rod photoreceptor complex that includes NR2E3, PIAS3, NRL, CRX and/or NR1D1. Binds direcly in the complex with CRX, PIAS3 and NR1D1 (By similarity). Interacts (via the DNA-binding domain) with CRX (via its DNA binding domain); the interaction represses S- and M-cone opsin expression.By similarity1 Publication

Protein-protein interaction databases

BioGridi204819. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliQ9QXZ7.
SMRiQ9QXZ7. Positions 38-395.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi356 – 3605Poly-Leu

Sequence similaritiesi

Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri40 – 6021NR C4-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri76 – 10126NR C4-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG277339.
GeneTreeiENSGT00760000118948.
HOGENOMiHOG000260820.
HOVERGENiHBG005606.
InParanoidiQ9QXZ7.
KOiK08546.
OMAiMLSQHTK.
OrthoDBiEOG7WT41C.
PhylomeDBiQ9QXZ7.
TreeFamiTF315716.

Family and domain databases

Gene3Di1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProiIPR003068. COUP_TF.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamiPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSiPR01282. COUPTNFACTOR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTiSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMiSSF48508. SSF48508. 2 hits.
PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QXZ7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSSTVAASTM PVSVAASKKE SPGRWGLGED PTGVGPSLQC RVCGDSSSGK
60 70 80 90 100
HYGIYACNGC SGFFKRSVRR RLIYRCQVGA GMCPVDKAHR NQCQACRLKK
110 120 130 140 150
CLQAGMNQDA VQNERQPRSM AQVHLDAMET GSDPRSEPVV ASPALAGPSP
160 170 180 190 200
RGPTSVSATR AMGHHFMASL ITAETCAKLE PEDAEENIDV TSNDPEFPAS
210 220 230 240 250
PCSLDGIHET SARLLFMAVK WAKNLPVFSN LPFRDQVILL EEAWNELFLL
260 270 280 290 300
GAIQWSLPLD SCPLLAPPEA SGSSQGRLAL ASAETRFLQE TISRFRALAV
310 320 330 340 350
DPTEFACLKA LVLFKPETRG LKDPEHVEAL QDQSQVMLSQ HSKAHHPSQP
360 370 380 390
VRFGKLLLLL PSLRFLTAER IELLFFRKTI GNTPMEKLLC DMFKN
Length:395
Mass (Da):43,177
Last modified:May 1, 2000 - v1
Checksum:iD8E391CF24919A68
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148129 mRNA. Translation: AAF22228.1.
AF204053 mRNA. Translation: AAF69682.1.
BC017521 mRNA. Translation: AAH17521.1.
CCDSiCCDS23255.1.
RefSeqiNP_038736.1. NM_013708.4.
UniGeneiMm.103641.

Genome annotation databases

EnsembliENSMUST00000034831; ENSMUSP00000034831; ENSMUSG00000032292.
GeneIDi23958.
KEGGimmu:23958.
UCSCiuc009pyu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF148129 mRNA. Translation: AAF22228.1 .
AF204053 mRNA. Translation: AAF69682.1 .
BC017521 mRNA. Translation: AAH17521.1 .
CCDSi CCDS23255.1.
RefSeqi NP_038736.1. NM_013708.4.
UniGenei Mm.103641.

3D structure databases

ProteinModelPortali Q9QXZ7.
SMRi Q9QXZ7. Positions 38-395.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204819. 1 interaction.

PTM databases

PhosphoSitei Q9QXZ7.

Proteomic databases

PRIDEi Q9QXZ7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000034831 ; ENSMUSP00000034831 ; ENSMUSG00000032292 .
GeneIDi 23958.
KEGGi mmu:23958.
UCSCi uc009pyu.1. mouse.

Organism-specific databases

CTDi 10002.
MGIi MGI:1346317. Nr2e3.

Phylogenomic databases

eggNOGi NOG277339.
GeneTreei ENSGT00760000118948.
HOGENOMi HOG000260820.
HOVERGENi HBG005606.
InParanoidi Q9QXZ7.
KOi K08546.
OMAi MLSQHTK.
OrthoDBi EOG7WT41C.
PhylomeDBi Q9QXZ7.
TreeFami TF315716.

Miscellaneous databases

NextBioi 303796.
PROi Q9QXZ7.
SOURCEi Search...

Gene expression databases

Bgeei Q9QXZ7.
CleanExi MM_NR2E3.
ExpressionAtlasi Q9QXZ7. differential.
Genevestigatori Q9QXZ7.

Family and domain databases

Gene3Di 1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProi IPR003068. COUP_TF.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view ]
Pfami PF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view ]
PRINTSi PR01282. COUPTNFACTOR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTi SM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view ]
SUPFAMi SSF48508. SSF48508. 2 hits.
PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Retina-specific nuclear receptor: a potential regulator of cellular retinaldehyde-binding protein expressed in retinal pigment epithelium and Muller glial cells."
    Chen F., Figueroa D.J., Marmorstein A.D., Zhang Q., Petrukhin K., Caskey C.T., Austin C.P.
    Proc. Natl. Acad. Sci. U.S.A. 96:15149-15154(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Retina.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
    Strain: C57BL/6J.
    Tissue: Retina.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  4. "Photoreceptor-specific nuclear receptor NR2E3 functions as a transcriptional activator in rod photoreceptors."
    Cheng H., Khanna H., Oh E.C., Hicks D., Mitton K.P., Swaroop A.
    Hum. Mol. Genet. 13:1563-1575(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
  5. "The rod photoreceptor-specific nuclear receptor Nr2e3 represses transcription of multiple cone-specific genes."
    Chen J., Rattner A., Nathans J.
    J. Neurosci. 25:118-129(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  6. "Pias3-dependent SUMOylation directs rod photoreceptor development."
    Onishi A., Peng G.-H., Hsu C., Alexis U., Chen S., Blackshaw S.
    Neuron 61:234-246(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUMOYLATION AT LYS-178, INTERACTION WITH CRX AND PIAS3, FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-178; LYS-315 AND LYS-322.
  7. "Nuclear receptor Rev-erb alpha (Nr1d1) functions in concert with Nr2e3 to regulate transcriptional networks in the retina."
    Mollema N.J., Yuan Y., Jelcick A.S., Sachs A.J., von Alpen D., Schorderet D., Escher P., Haider N.B.
    PLoS ONE 6:E17494-E17494(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiNR2E3_MOUSE
AccessioniPrimary (citable) accession number: Q9QXZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3