Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9QXZ7 (NR2E3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Photoreceptor-specific nuclear receptor
Alternative name(s):
Nuclear receptor subfamily 2 group E member 3
Retina-specific nuclear receptor
Gene names
Name:Nr2e3
Synonyms:Pnr, Rnr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length395 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Orphan nuclear receptor of retinal photoreceptor cells. Transcriptional factor that is an activator of rod development and repressor of cone development. Binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. Enhances rhodopsin expression. Represses M- and S-cone opsin expression. Ref.4 Ref.5 Ref.6 Ref.7

Subunit structure

Homodimer. Interacts with PIAS3; the interaction sumoylates NR2E3 and promotes repression of cone-specific gene transcription and activation of rod-specific genes. Component of a rod photoreceptor complex that includes NR2E3, PIAS3, NRL, CRX and/or NR1D1. Binds direcly in the complex with CRX, PIAS3 and NR1D1 By similarity. Interacts (via the DNA-binding domain) with CRX (via its DNA binding domain); the interaction represses S- and M-cone opsin expression. Ref.6

Subcellular location

Nucleus Ref.4 Ref.6.

Tissue specificity

Retina. Rod-specific. Expressed in the outer nuclear lyer of the mature retina. Ref.1 Ref.2 Ref.4 Ref.5 Ref.7

Developmental stage

Expression found as early as E18 in developing retina. From P3 to P6, expression increases in developing rods. Expression, thereafter, in the future inner nuclear layer migrating to the final destination of the outer nuclear layer. In the mature retina, exclusively expressed in rods. Ref.5

Post-translational modification

Di- and tri-sumoylated in developing retina. PIAS3-mediated sumoylation promotes repression of cone-specific gene expression and activation of rod-specific genes. Sumoylation on Lys-178 appears to be the main site. Ref.6

Disruption phenotype

Defects in Nr2e3 are the cause of the retinal degeneration type 7 (Rd7) phenotype characterized by excessive blue cones and loss of rods. Ref.2 Ref.5

Sequence similarities

Belongs to the nuclear hormone receptor family. NR2 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionReceptor
   PTMIsopeptide bond
Phosphoprotein
Ubl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processeye photoreceptor cell development

Inferred from mutant phenotype PubMed 21052544. Source: MGI

negative regulation of cell proliferation

Inferred from mutant phenotype PubMed 12407160. Source: MGI

negative regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 15689355. Source: MGI

positive regulation of rhodopsin gene expression

Inferred from genetic interaction PubMed 15689355. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 15689355. Source: MGI

retina development in camera-type eye

Inferred from mutant phenotype PubMed 12407160PubMed 21052544. Source: MGI

   Cellular_componentnucleus

Inferred from direct assay PubMed 15689355. Source: MGI

transcription factor complex

Inferred from direct assay PubMed 15689355. Source: MGI

   Molecular_functionligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 15689355. Source: MGI

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 395395Photoreceptor-specific nuclear receptor
PRO_0000053600

Regions

DNA binding37 – 11377Nuclear receptor
Zinc finger40 – 6021NR C4-type
Zinc finger76 – 10126NR C4-type
Compositional bias356 – 3605Poly-Leu

Amino acid modifications

Modified residue3181Phosphothreonine By similarity
Cross-link178Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) Ref.6
Cross-link315Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-link322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Experimental info

Mutagenesis1781K → R: Greatly reduced levels of PIAS3-mediated sumoylation. Completely abolishes sumoylation; when associated with R-315 and R-322. Ref.6
Mutagenesis3151K → R: Little change in sumoylation. Completely abolishes sumoylation; when associated with R-178 and R-322. Ref.6
Mutagenesis3221K → R: Little change in sumoylation. Completely abolishes sumoylation; when associated with R-178 and R-315. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Q9QXZ7 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: D8E391CF24919A68

FASTA39543,177
        10         20         30         40         50         60 
MSSTVAASTM PVSVAASKKE SPGRWGLGED PTGVGPSLQC RVCGDSSSGK HYGIYACNGC 

        70         80         90        100        110        120 
SGFFKRSVRR RLIYRCQVGA GMCPVDKAHR NQCQACRLKK CLQAGMNQDA VQNERQPRSM 

       130        140        150        160        170        180 
AQVHLDAMET GSDPRSEPVV ASPALAGPSP RGPTSVSATR AMGHHFMASL ITAETCAKLE 

       190        200        210        220        230        240 
PEDAEENIDV TSNDPEFPAS PCSLDGIHET SARLLFMAVK WAKNLPVFSN LPFRDQVILL 

       250        260        270        280        290        300 
EEAWNELFLL GAIQWSLPLD SCPLLAPPEA SGSSQGRLAL ASAETRFLQE TISRFRALAV 

       310        320        330        340        350        360 
DPTEFACLKA LVLFKPETRG LKDPEHVEAL QDQSQVMLSQ HSKAHHPSQP VRFGKLLLLL 

       370        380        390 
PSLRFLTAER IELLFFRKTI GNTPMEKLLC DMFKN 

« Hide

References

« Hide 'large scale' references
[1]"Retina-specific nuclear receptor: a potential regulator of cellular retinaldehyde-binding protein expressed in retinal pigment epithelium and Muller glial cells."
Chen F., Figueroa D.J., Marmorstein A.D., Zhang Q., Petrukhin K., Caskey C.T., Austin C.P.
Proc. Natl. Acad. Sci. U.S.A. 96:15149-15154(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Retina.
[2]"A deletion in a photoreceptor-specific nuclear receptor mRNA causes retinal degeneration in the rd7 mouse."
Akhmedov N.B., Piriev N.I., Chang B., Rapoport A.L., Hawes N.L., Nishina P.M., Nusinowitz S., Heckenlively J.R., Roderick T.H., Kozak C.A., Danciger M., Davisson M.T., Farber D.B.
Proc. Natl. Acad. Sci. U.S.A. 97:5551-5556(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
Strain: C57BL/6J.
Tissue: Retina.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[4]"Photoreceptor-specific nuclear receptor NR2E3 functions as a transcriptional activator in rod photoreceptors."
Cheng H., Khanna H., Oh E.C., Hicks D., Mitton K.P., Swaroop A.
Hum. Mol. Genet. 13:1563-1575(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[5]"The rod photoreceptor-specific nuclear receptor Nr2e3 represses transcription of multiple cone-specific genes."
Chen J., Rattner A., Nathans J.
J. Neurosci. 25:118-129(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[6]"Pias3-dependent SUMOylation directs rod photoreceptor development."
Onishi A., Peng G.-H., Hsu C., Alexis U., Chen S., Blackshaw S.
Neuron 61:234-246(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUMOYLATION AT LYS-178, INTERACTION WITH CRX AND PIAS3, FUNCTION, SUBCELLULAR LOCATION, MUTAGENESIS OF LYS-178; LYS-315 AND LYS-322.
[7]"Nuclear receptor Rev-erb alpha (Nr1d1) functions in concert with Nr2e3 to regulate transcriptional networks in the retina."
Mollema N.J., Yuan Y., Jelcick A.S., Sachs A.J., von Alpen D., Schorderet D., Escher P., Haider N.B.
PLoS ONE 6:E17494-E17494(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF148129 mRNA. Translation: AAF22228.1.
AF204053 mRNA. Translation: AAF69682.1.
BC017521 mRNA. Translation: AAH17521.1.
RefSeqNP_038736.1. NM_013708.4.
UniGeneMm.103641.

3D structure databases

ProteinModelPortalQ9QXZ7.
SMRQ9QXZ7. Positions 38-395.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204819. 1 interaction.

PTM databases

PhosphoSiteQ9QXZ7.

Proteomic databases

PRIDEQ9QXZ7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034831; ENSMUSP00000034831; ENSMUSG00000032292.
GeneID23958.
KEGGmmu:23958.
UCSCuc009pyu.1. mouse.

Organism-specific databases

CTD10002.
MGIMGI:1346317. Nr2e3.

Phylogenomic databases

eggNOGNOG277339.
GeneTreeENSGT00720000108595.
HOGENOMHOG000260820.
HOVERGENHBG005606.
InParanoidQ9QXZ7.
KOK08546.
OMAMLSQHTK.
OrthoDBEOG7WT41C.
PhylomeDBQ9QXZ7.
TreeFamTF315716.

Gene expression databases

BgeeQ9QXZ7.
CleanExMM_NR2E3.
GenevestigatorQ9QXZ7.

Family and domain databases

Gene3D1.10.565.10. 2 hits.
3.30.50.10. 1 hit.
InterProIPR003068. COUP_TF.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSPR01282. COUPTNFACTOR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 2 hits.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio303796.
PROQ9QXZ7.
SOURCESearch...

Entry information

Entry nameNR2E3_MOUSE
AccessionPrimary (citable) accession number: Q9QXZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot