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Protein

Photoreceptor-specific nuclear receptor

Gene

Nr2e3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Orphan nuclear receptor of retinal photoreceptor cells. Transcriptional factor that is an activator of rod development and repressor of cone development. Binds the promoter region of a number of rod- and cone-specific genes, including rhodopsin, M- and S-opsin and rod-specific phosphodiesterase beta subunit. Enhances rhodopsin expression. Represses M- and S-cone opsin expression.4 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi37 – 113Nuclear receptorPROSITE-ProRule annotationAdd BLAST77
Zinc fingeri40 – 60NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri76 – 101NR C4-typePROSITE-ProRule annotationAdd BLAST26

GO - Molecular functioni

GO - Biological processi

  • eye photoreceptor cell development Source: MGI
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of rhodopsin gene expression Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • retina development in camera-type eye Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding, Receptor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-383280 Nuclear Receptor transcription pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Photoreceptor-specific nuclear receptor
Alternative name(s):
Nuclear receptor subfamily 2 group E member 3
Retina-specific nuclear receptor
Gene namesi
Name:Nr2e3
Synonyms:Pnr, Rnr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1346317 Nr2e3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Defects in Nr2e3 are the cause of the retinal degeneration type 7 (Rd7) phenotype characterized by excessive blue cones and loss of rods.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi178K → R: Greatly reduced levels of PIAS3-mediated sumoylation. Completely abolishes sumoylation; when associated with R-315 and R-322. 1 Publication1
Mutagenesisi315K → R: Little change in sumoylation. Completely abolishes sumoylation; when associated with R-178 and R-322. 1 Publication1
Mutagenesisi322K → R: Little change in sumoylation. Completely abolishes sumoylation; when associated with R-178 and R-315. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000536001 – 395Photoreceptor-specific nuclear receptorAdd BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki178Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki315Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
Cross-linki322Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

Post-translational modificationi

Di- and tri-sumoylated in developing retina. PIAS3-mediated sumoylation promotes repression of cone-specific gene expression and activation of rod-specific genes. Sumoylation on Lys-178 appears to be the main site.1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ9QXZ7
PRIDEiQ9QXZ7

PTM databases

iPTMnetiQ9QXZ7
PhosphoSitePlusiQ9QXZ7

Expressioni

Tissue specificityi

Retina. Rod-specific. Expressed in the outer nuclear lyer of the mature retina.5 Publications

Developmental stagei

Expression found as early as E18 in developing retina. From P3 to P6, expression increases in developing rods. Expression, thereafter, in the future inner nuclear layer migrating to the final destination of the outer nuclear layer. In the mature retina, exclusively expressed in rods.1 Publication

Gene expression databases

BgeeiENSMUSG00000032292
CleanExiMM_NR2E3
ExpressionAtlasiQ9QXZ7 baseline and differential
GenevisibleiQ9QXZ7 MM

Interactioni

Subunit structurei

Homodimer. Interacts with PIAS3; the interaction sumoylates NR2E3 and promotes repression of cone-specific gene transcription and activation of rod-specific genes. Component of a rod photoreceptor complex that includes NR2E3, PIAS3, NRL, CRX and/or NR1D1. Binds direcly in the complex with CRX, PIAS3 and NR1D1 (By similarity). Interacts (via the DNA-binding domain) with CRX (via its DNA binding domain); the interaction represses S- and M-cone opsin expression.By similarity1 Publication

Protein-protein interaction databases

BioGridi204819, 1 interactor
STRINGi10090.ENSMUSP00000034831

Structurei

3D structure databases

ProteinModelPortaliQ9QXZ7
SMRiQ9QXZ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini162 – 395NR LBDPROSITE-ProRule annotationAdd BLAST234

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi356 – 360Poly-Leu5

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri40 – 60NR C4-typePROSITE-ProRule annotationAdd BLAST21
Zinc fingeri76 – 101NR C4-typePROSITE-ProRule annotationAdd BLAST26

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3575 Eukaryota
ENOG410XRZC LUCA
GeneTreeiENSGT00760000118948
HOGENOMiHOG000260820
HOVERGENiHBG005606
InParanoidiQ9QXZ7
KOiK08546
OMAiHHHFMAS
OrthoDBiEOG091G0AV3
PhylomeDBiQ9QXZ7
TreeFamiTF315716

Family and domain databases

Gene3Di3.30.50.10, 1 hit
InterProiView protein in InterPro
IPR003068 COUP_TF
IPR035500 NHR_like_dom_sf
IPR000536 Nucl_hrmn_rcpt_lig-bd
IPR001723 Nuclear_hrmn_rcpt
IPR001628 Znf_hrmn_rcpt
IPR013088 Znf_NHR/GATA
PfamiView protein in Pfam
PF00104 Hormone_recep, 1 hit
PF00105 zf-C4, 1 hit
PRINTSiPR01282 COUPTNFACTOR
PR00398 STRDHORMONER
PR00047 STROIDFINGER
SMARTiView protein in SMART
SM00430 HOLI, 1 hit
SM00399 ZnF_C4, 1 hit
SUPFAMiSSF48508 SSF48508, 2 hits
PROSITEiView protein in PROSITE
PS51843 NR_LBD, 1 hit
PS00031 NUCLEAR_REC_DBD_1, 1 hit
PS51030 NUCLEAR_REC_DBD_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QXZ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTVAASTM PVSVAASKKE SPGRWGLGED PTGVGPSLQC RVCGDSSSGK
60 70 80 90 100
HYGIYACNGC SGFFKRSVRR RLIYRCQVGA GMCPVDKAHR NQCQACRLKK
110 120 130 140 150
CLQAGMNQDA VQNERQPRSM AQVHLDAMET GSDPRSEPVV ASPALAGPSP
160 170 180 190 200
RGPTSVSATR AMGHHFMASL ITAETCAKLE PEDAEENIDV TSNDPEFPAS
210 220 230 240 250
PCSLDGIHET SARLLFMAVK WAKNLPVFSN LPFRDQVILL EEAWNELFLL
260 270 280 290 300
GAIQWSLPLD SCPLLAPPEA SGSSQGRLAL ASAETRFLQE TISRFRALAV
310 320 330 340 350
DPTEFACLKA LVLFKPETRG LKDPEHVEAL QDQSQVMLSQ HSKAHHPSQP
360 370 380 390
VRFGKLLLLL PSLRFLTAER IELLFFRKTI GNTPMEKLLC DMFKN
Length:395
Mass (Da):43,177
Last modified:May 1, 2000 - v1
Checksum:iD8E391CF24919A68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF148129 mRNA Translation: AAF22228.1
AF204053 mRNA Translation: AAF69682.1
BC017521 mRNA Translation: AAH17521.1
CCDSiCCDS23255.1
RefSeqiNP_038736.1, NM_013708.4
UniGeneiMm.103641

Genome annotation databases

EnsembliENSMUST00000034831; ENSMUSP00000034831; ENSMUSG00000032292
GeneIDi23958
KEGGimmu:23958
UCSCiuc009pyu.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNR2E3_MOUSE
AccessioniPrimary (citable) accession number: Q9QXZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health