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Protein

Microtubule-actin cross-linking factor 1

Gene

Macf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Isoform 2 is a F-actin-binding protein which may play a role in cross-linking actin to other cytoskeletal proteins and also binds to microtubules. Plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. Acts as a positive regulator of Wnt receptor signaling pathway and is involved in the translocation of AXIN1 and its associated complex (composed of APC, CTNNB1 and GSK3B) from the cytoplasm to the cell membrane. Has actin-regulated ATPase activity and is essential for controlling focal adhesions (FAs) assembly and dynamics. May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with GOLGA4. Plays a key role in wound healing and epidermal cell migration. Required for efficient upward migration of bulge cells in response to wounding and this function is primarily rooted in its ability to coordinate MT dynamics and polarize hair follicle stem cells.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi7014 – 7025121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi7050 – 7061122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • actin binding Source: MGI
  • ATPase activity Source: UniProtKB
  • calcium ion binding Source: InterPro
  • microtubule binding Source: MGI

GO - Biological processi

  • cell cycle arrest Source: InterPro
  • establishment or maintenance of cell polarity Source: MGI
  • Golgi to plasma membrane protein transport Source: UniProtKB
  • mesoderm formation Source: MGI
  • positive regulation of Wnt signaling pathway Source: UniProtKB
  • posttranslational protein targeting to membrane Source: MGI
  • protein localization Source: MGI
  • regulation of epithelial cell migration Source: UniProtKB
  • regulation of focal adhesion assembly Source: UniProtKB
  • regulation of microtubule-based process Source: UniProtKB
  • Wnt signaling pathway Source: MGI
  • wound healing Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Keywords - Ligandi

Actin-binding, Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-actin cross-linking factor 1
Alternative name(s):
Actin cross-linking family 7
Gene namesi
Name:Macf1
Synonyms:Acf7, Aclp7, Macf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:108559. Macf1.

Subcellular locationi

Isoform 2 :
  • Cytoplasm
  • Cytoplasmcytoskeleton
  • Golgi apparatus By similarity
  • Cell membrane By similarity
  • Cell projectionruffle membrane By similarity

  • Note: APC controls its localization to the cell membrane which is critical for its function in microtubule stabilization (By similarity). Localizes to the tips of microtubules. The phosphorylated form is found in the cytoplasm while the non-phosphorylated form associates with the microtubules.By similarity
Isoform 1 :

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • cell cortex Source: MGI
  • cytoplasm Source: UniProtKB
  • Golgi apparatus Source: UniProtKB
  • membrane Source: UniProtKB
  • microtubule Source: UniProtKB
  • microtubule cytoskeleton Source: MGI
  • neuronal postsynaptic density Source: MGI
  • ruffle membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, Microtubule

Pathology & Biotechi

Disruption phenotypei

Mice die at the gastrulation stage and display developmental retardation at E7.5 with defects in the formation of the primitive streak, node, and mesoderm.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 73547354Microtubule-actin cross-linking factor 1PRO_0000073451Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei4 – 41PhosphoserineBy similarity
Modified residuei35 – 351PhosphoserineCombined sources
Modified residuei57 – 571PhosphoserineCombined sources
Modified residuei280 – 2801PhosphoserineCombined sources
Modified residuei1376 – 13761PhosphoserineCombined sources
Modified residuei2077 – 20771PhosphoserineCombined sources
Modified residuei2081 – 20811PhosphoserineCombined sources
Modified residuei3082 – 30821PhosphoserineCombined sources
Modified residuei3085 – 30851PhosphoserineCombined sources
Modified residuei3889 – 38891PhosphoserineCombined sources
Modified residuei4458 – 44581PhosphoserineCombined sources
Modified residuei4483 – 44831PhosphoserineCombined sources
Modified residuei4921 – 49211PhosphoserineBy similarity
Modified residuei5394 – 53941PhosphothreonineCombined sources
Modified residuei5988 – 59881PhosphoserineCombined sources
Modified residuei6166 – 61661N6-acetyllysineBy similarity
Modified residuei6923 – 69231PhosphoserineCombined sources
Modified residuei7220 – 72201PhosphothreonineBy similarity
Modified residuei7258 – 72581PhosphoserineBy similarity
Modified residuei7296 – 72961PhosphoserineCombined sources
Modified residuei7299 – 72991PhosphoserineCombined sources
Isoform 4 (identifier: Q9QXZ0-4)
Modified residuei42 – 421PhosphothreonineCombined sources

Post-translational modificationi

Phosphorylated on serine residues in the C-terminal tail by GSK3B. Phosphorylation inhibits microtubule-binding and this plays a critical role in bulge stem cell migration and skin wound repair. Wnt-signaling can repress phosphorylation.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9QXZ0.
MaxQBiQ9QXZ0.
PaxDbiQ9QXZ0.
PRIDEiQ9QXZ0.

PTM databases

iPTMnetiQ9QXZ0.
PhosphoSiteiQ9QXZ0.

Expressioni

Tissue specificityi

Enriched in the hair follicle stem cells (at protein level). Isoform 1 and isoform 2 are ubiquitous expressed, with higher levels seen in lung, heart, thymus, spleen and brain.3 Publications

Developmental stagei

Isoform 2 is highly expressed in neuronal tissues and the foregut of embryonic day 8.5 (E8.5) embryos and the head fold and primitive streak of E7.5 embryos (at protein level). Isoform 1 is expressed throughout the development of the embryo.1 Publication

Gene expression databases

BgeeiQ9QXZ0.
CleanExiMM_MACF1.

Interactioni

Subunit structurei

Isoform 2 interacts with AXIN1, LRP6 and GOLGA4 (By similarity). Isoform 2 is found in a complex composed of MACF1, APC, AXIN1, CTNNB1 and GSK3B (By similarity). Isoform 2 interacts with MAPRE1, CLASP1 AND CLASP2.By similarity1 Publication

GO - Molecular functioni

  • actin binding Source: MGI
  • microtubule binding Source: MGI

Protein-protein interaction databases

IntActiQ9QXZ0. 9 interactions.
MINTiMINT-1634787.
STRINGi10090.ENSMUSP00000095507.

Structurei

3D structure databases

ProteinModelPortaliQ9QXZ0.
SMRiQ9QXZ0. Positions 74-298.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 295295Actin-bindingAdd
BLAST
Domaini78 – 181104CH 1PROSITE-ProRule annotationAdd
BLAST
Repeati148 – 17124LRR 1Add
BLAST
Domaini194 – 295102CH 2PROSITE-ProRule annotationAdd
BLAST
Repeati377 – 39923LRR 3Add
BLAST
Repeati441 – 46424LRR 4Add
BLAST
Domaini871 – 92353SH3Add
BLAST
Repeati1050 – 107324LRR 5Add
BLAST
Repeati1128 – 115427LRR 6Add
BLAST
Repeati1187 – 121024LRR 7Add
BLAST
Repeati1257 – 128226LRR 8Add
BLAST
Repeati1577 – 161943Plectin 1Add
BLAST
Repeati1654 – 169643Plectin 2Add
BLAST
Repeati1769 – 180941Plectin 3Add
BLAST
Repeati1811 – 184838Plectin 4Add
BLAST
Repeati1855 – 188531Plectin 5Add
BLAST
Repeati2276 – 231641Plectin 6Add
BLAST
Repeati2352 – 239342Plectin 7Add
BLAST
Repeati2394 – 242532Plectin 8Add
BLAST
Repeati2487 – 252842Plectin 9Add
BLAST
Repeati2671 – 271545Plectin 10Add
BLAST
Repeati3225 – 324420LRR 9Add
BLAST
Repeati3606 – 363025LRR 10Add
BLAST
Repeati3657 – 368125LRR 11Add
BLAST
Repeati3845 – 392076Spectrin 1Add
BLAST
Repeati3898 – 392023LRR 12Add
BLAST
Repeati3962 – 4070109Spectrin 2Add
BLAST
Repeati4087 – 411226LRR 13Add
BLAST
Repeati4223 – 424927LRR 14Add
BLAST
Repeati4428 – 4536109Spectrin 3Add
BLAST
Repeati4473 – 449624LRR 15Add
BLAST
Repeati4563 – 458321LRR 16Add
BLAST
Repeati4728 – 475124LRR 17Add
BLAST
Repeati4759 – 4863105Spectrin 4Add
BLAST
Repeati5010 – 503526LRR 18Add
BLAST
Repeati5131 – 515323LRR 19Add
BLAST
Repeati5195 – 5300106Spectrin 5Add
BLAST
Repeati5240 – 526324LRR 20Add
BLAST
Repeati5307 – 5409103Spectrin 6Add
BLAST
Repeati5414 – 550693Spectrin 7Add
BLAST
Repeati5631 – 5735105Spectrin 8Add
BLAST
Repeati5654 – 567825LRR 21Add
BLAST
Repeati5742 – 5844103Spectrin 9Add
BLAST
Repeati5763 – 578725LRR 22Add
BLAST
Repeati5961 – 6066106Spectrin 10Add
BLAST
Repeati6071 – 6175105Spectrin 11Add
BLAST
Repeati6181 – 6284104Spectrin 12Add
BLAST
Repeati6289 – 6395107Spectrin 13Add
BLAST
Repeati6400 – 6503104Spectrin 14Add
BLAST
Repeati6452 – 647524LRR 23Add
BLAST
Repeati6508 – 6614107Spectrin 15Add
BLAST
Repeati6621 – 6722102Spectrin 16Add
BLAST
Repeati6726 – 6830105Spectrin 17Add
BLAST
Domaini7001 – 703636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini7037 – 707236EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini7077 – 715579GARPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni7077 – 7354278C-terminal tailAdd
BLAST
Regioni7279 – 7294164 X 4 AA tandem repeats of [GS]-S-R-[AR]Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi700 – 7034Poly-Glu
Compositional biasi1414 – 14174Poly-Glu
Compositional biasi1986 – 19894Poly-Leu
Compositional biasi3521 – 357656Gln-richAdd
BLAST
Compositional biasi7188 – 7325138Ser-richAdd
BLAST

Domaini

The C-terminal tail is required for phosphorylation by GSK3B and for microtubule-binding.

Sequence similaritiesi

Belongs to the plakin or cytolinker family.Curated
Contains 1 actin-binding domain.Curated
Contains 2 CH (calponin-homology) domains.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation
Contains 1 GAR domain.PROSITE-ProRule annotation
Contains 23 LRR (leucine-rich) repeats.Curated
Contains 10 plectin repeats.Curated
Contains 1 SH3 domain.Curated
Contains 17 spectrin repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG0516. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000049054.
HOVERGENiHBG031127.
InParanoidiQ9QXZ0.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.418.10. 2 hits.
3.30.920.20. 1 hit.
3.90.1290.10. 5 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR003108. GAS2_dom.
IPR028408. Macf1.
IPR001101. Plectin_repeat.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PANTHERiPTHR11915:SF37. PTHR11915:SF37. 6 hits.
PfamiPF00307. CH. 2 hits.
PF13499. EF-hand_7. 1 hit.
PF02187. GAS2. 1 hit.
PF00681. Plectin. 8 hits.
PF00435. Spectrin. 18 hits.
[Graphical view]
SMARTiSM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00243. GAS2. 1 hit.
SM00250. PLEC. 19 hits.
SM00150. SPEC. 34 hits.
[Graphical view]
SUPFAMiSSF143575. SSF143575. 1 hit.
SSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
SSF75399. SSF75399. 6 hits.
PROSITEiPS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS51460. GAR. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QXZ0-1) [UniParc]FASTAAdd to basket

Also known as: Macf1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSSSDEETLS ERSCRSERSC RSERSYRSER SGSLSPCPPG DTLPWNLPLH
60 70 80 90 100
EQKKRKSQDS VLDPAERAVV RVADERDRVQ KKTFTKWVNK HLMKVRKHIN
110 120 130 140 150
DLYEDLRDGH NLISLLEVLS GIKLPREKGR MRFHRLQNVQ IALDFLKQRQ
160 170 180 190 200
VKLVNIRNDD ITDGNPKLTL GLIWTIILHF QISDIYISGE SGDMSAKEKL
210 220 230 240 250
LLWTQKVTAG YTGVKCTNFS SCWSDGKMFN ALIHRYRPDL VDMERVQVQS
260 270 280 290 300
NRENLEQAFE VAERLGVTRL LDAEDVDVPS PDEKSVITYV SSIYDAFPKV
310 320 330 340 350
PEGGEGISAT EVDSRWQEYQ SRVDSLIPWI RQHTILMSDK SFPQNPVELK
360 370 380 390 400
ALYNQYIHFK ETEILAKERE KGRIKELYKL LEVWIEFGRI KLPQGYHPNH
410 420 430 440 450
VEEEWGKLIV EMLEREKSLR PAVERLELLL QIANKIQNGA LNCEEKLTLA
460 470 480 490 500
KNTLQADAAH LESGQPVQCE SDVIMYIQEC EGLIRQLQVD LQILRDEKYY
510 520 530 540 550
QLEELAFRVM RLQDELVTLR LECTNLYRKG HFSSLELVPP STLTTTHLKA
560 570 580 590 600
EPLNKTTHSS STSWFRKPMT RTELVSISSS EDEGNLRFVY ELLSWVEEMQ
610 620 630 640 650
MKLERAEWGN DLPSVELQLE TQQHIHTSVE ELGSSVKEAR LYEGKMSQNF
660 670 680 690 700
HTSYVETLGK LETQYCKLKE TSSFRMRHLQ SLHKFVSRAT AELIWLNGKE
710 720 730 740 750
EEELACDWSD SNPNISAKKT YFSELTMELE GKQDVFRSLQ DTAEVLSLEN
760 770 780 790 800
HPAKQTVEAY SAAVQSQLQW MKQLCLCVEQ HVKENAAYFQ FFSDARDLES
810 820 830 840 850
FLRNLQDSIK RKYTCDRSTS LSRLEDLLQD SMDEKEQLIQ SKSSVASLVG
860 870 880 890 900
RSKTIVQLKP RNPDHVLKST LSVKAICDYR QIEITICKND ECVLEDNSQR
910 920 930 940 950
TKWKVISPTG NEAMVPSVCF LIPPPNKEAI EMASRVEQSY QKVMALWHQL
960 970 980 990 1000
HINTKSLISW NYLRKDLDTV QTWSLEKLRS LAPGECHQVM KNLQAHYEDF
1010 1020 1030 1040 1050
LQDSHDSALF SVADRLRIEE EVEACKAHFQ HLMKSLENED KEETLAKVYI
1060 1070 1080 1090 1100
SELKNIRLLL EECEQRLLKQ IQSPASSKTD RDARQDITLR IAEQEHTQED
1110 1120 1130 1140 1150
LQHLRSDLDA ISMKCNVFLQ QSPSGSSATT LRSELNLMVE KMDHVYGLST
1160 1170 1180 1190 1200
VYLNKLKTID VIVRSMQDAE LLVKGYEIKL SQEEAVPADL SALESHRTTL
1210 1220 1230 1240 1250
QHWLSDVKDK NSVFSVLDEE ITKAKKVAEQ LRHPASEPNL DLERYQEKGS
1260 1270 1280 1290 1300
QLQERWHRVI AQLETRQSEV ESIQEVLRDY RACHGTLIKW IEETTAQQEM
1310 1320 1330 1340 1350
MKPGQAEDSR VLSEQLSQQT ELFAEIERNQ TKLDQCQKFS QQYSTIVKDY
1360 1370 1380 1390 1400
ELQLMTYKAF VESQQKSPGK RRRMISSSDA ITQEFMDLRT RYTALVTLTT
1410 1420 1430 1440 1450
QHVKYISDAL RRLEEEEKVV EEEKQEHVEK VKDLLGWVST LARNTQGTTT
1460 1470 1480 1490 1500
SSHTSASADI EKAILEQQVL AEELTTKKEQ VSEAIKTSQI FLAKHGHKLS
1510 1520 1530 1540 1550
EGEKEQISEQ LRVLNKTYHD LCDGSANQLQ QLQSELAQQT EQKGCRAVAG
1560 1570 1580 1590 1600
VIDLGTVEIF PIFRAMQKGL IDQDTGLVLL ESQIIMSGLI DPENSEKLSL
1610 1620 1630 1640 1650
EEGLTRNFIN LPIYQQLLGL RDSLSLVSRL TGTLGSLSVV EAIEKKIISE
1660 1670 1680 1690 1700
RLGLKVLEVH LATGGFSLPP SENCINLEEA FHQGFIASSL HSELQSHLRS
1710 1720 1730 1740 1750
SKNLIDPNTA EKVGLLDLMQ RCIIHQESGL KLLPVKQLAG GMVSLKSGRK
1760 1770 1780 1790 1800
VSIFRAVQEG LIDRQVTVRL LEAQLFAGGI VDPRTGHRLT VEEAVRHNLI
1810 1820 1830 1840 1850
DQDMACAILI RQLQTGGIID TVTGDRMTID EAVTNNLVAA KIALVILESL
1860 1870 1880 1890 1900
WSFMGLLLPE SGEILPITDA LEQGIVSTEL AHKILHNRQQ IEALFLPTLT
1910 1920 1930 1940 1950
EIWSWEKATE SGILDKDLVN NLRSVCIPDM MPHIQLADSA EQSKVGFAAG
1960 1970 1980 1990 2000
KPPVSGPREE GSSHGEKLLF QLMTHSYIHA HTGQRLLLLD QELVEMLTSR
2010 2020 2030 2040 2050
DDCQVILPEV FEIQHQRLNT SEALQELYTG TISQISSAKH PRKPCESQFL
2060 2070 2080 2090 2100
SQNKDYPSQE NCTEAKGERS VVGIECSPAE SPERELFLKE QEAIIENVGS
2110 2120 2130 2140 2150
LKVINKVKLK LQRPLLGSRK EEQAETLREE NISGDPLLVE CPEESEGKDL
2160 2170 2180 2190 2200
STEKSKCQTP TKCSFTCHKE QVKTIKDIPS ETGTSLIKSQ NQMSQFQVDT
2210 2220 2230 2240 2250
SVGLRSEFKS EHDMNVNSLE KELKEELLVK DGHKQSQEGQ SVADGQTVAL
2260 2270 2280 2290 2300
EKTDTEDNAD EPALLHSSPF EDATLSTLSA QLQDGGIFNE ETGQKLLLNE
2310 2320 2330 2340 2350
AIAQGLVSSH TAVKLMGKLN MFRGFFDSQT CESLTTEEVI DEGLMDEKLL
2360 2370 2380 2390 2400
YNVLMSDKAI SGILDPRTHS LCSVKEAVAA GLLDKETATR ILEGQVITGG
2410 2420 2430 2440 2450
IVDLKRGKKL SVTLASNLGL VDTADQTELI NLEKATKGRG AEKAVKERLI
2460 2470 2480 2490 2500
ELQMETAGLM DPESKAPLTV LQSIDRGILE REAAVYLLTK QVLDGGIIHH
2510 2520 2530 2540 2550
ISGLRLSVDN AFKHGLIGED MARQLRKVEN FIHYQFFHPQ TKEALSFSEA
2560 2570 2580 2590 2600
IKLDLVSPDL KREIQEIQDF SGNLGDFIYG QKLTLAKTNK EESLANKTEL
2610 2620 2630 2640 2650
PSGVMHGVID PENCTIIPYS ELVKKCRIDT ESGWRYLEVI PFSDIKDEAG
2660 2670 2680 2690 2700
NNVLTPPEAI QLGKVDFASA LKVLEAQANT GGIIDMATGK RVTLASALEK
2710 2720 2730 2740 2750
KLLDENMARI IASHQMLSGG IIDIYSDQRV TLNDAVEKRL ISPELAAMIQ
2760 2770 2780 2790 2800
VDPLAEQGGT GVCELKGDFL RKELLSESSK TPRESYSKEK HEAVLQAGSL
2810 2820 2830 2840 2850
CAPEKAGIRG SNGEKAEKGR KISVEMEGQR QDEKASSDSK VSASILSPFG
2860 2870 2880 2890 2900
FEGESSYQVS VTHPCSESCD LKPREETRSC MKKCAVVERD KVVTQIKMVS
2910 2920 2930 2940 2950
HVKQSTSGLD AEEARERQGR MVSKEQGSHY ETAGNLLSER SVRVDRRVRR
2960 2970 2980 2990 3000
EMGGEQSVQM SREAAVLSEE ESDQEVTIGD EPDSFVKSQS MKMIGNDKGK
3010 3020 3030 3040 3050
EAGIEKDISV VCKIEGFPSQ MTSKDASLTN QDALPFYTEG ETKTVNLCSI
3060 3070 3080 3090 3100
LKPGEKLSQE TASTVQKEPL SSEIPRPERL NSQESDEEPQ ISDVPHISKG
3110 3120 3130 3140 3150
DMAAQITTRQ ETTDVQDLYI TSKSSETKDK IFPSKNYIEK LHQEIPMDPT
3160 3170 3180 3190 3200
RSHKLKEATI STLETEGISY LDSSDIKSLC EDSKADHKSC GHQKSKVTTT
3210 3220 3230 3240 3250
QAKKSLEVVD LLVRDTEEGS SEDRVGQRGP RVLASLLPEK LPTRTVQSEN
3260 3270 3280 3290 3300
IRQHDAVIPA ISEIREEMAL SLPCSVVKVD GKIPKEKHKE ILGDEQGPFM
3310 3320 3330 3340 3350
AIPSGKGIEG VNPEPCRATQ NVFTRRLCLE HDEKLVSYLS LLRDIEMRTK
3360 3370 3380 3390 3400
QIQPLELNVA ELQDLLGQAK ELDRELKDLS TVVSQELECV DRIVISQPQE
3410 3420 3430 3440 3450
VPAQLLKALE KDAKNLQKSL DSVSDSWSSR FLHLQSAVEV KKATVLNRHK
3460 3470 3480 3490 3500
ELQGKLQDLR AWVGRASLTL NSKGCDTETD ADSLSHTLQP YKDMKQSMAE
3510 3520 3530 3540 3550
RKSQLDALAL DIQLFISEHP QDLSLQQNQE MLQFLSELQR SFQGLVEHTA
3560 3570 3580 3590 3600
AQKDVVQGHL QQVQQEVQVK TLQKQQDTCH KKLEDLCNWV GQAERALERH
3610 3620 3630 3640 3650
QGGSSSGKSS LLCAEPKVDL KDLQGDIQSH STSFATAVKD IEGFLEENQT
3660 3670 3680 3690 3700
KLSPQELTAL REKLHQAKEQ YEVLQERTRV AQKELEEAVT SALQQETEKS
3710 3720 3730 3740 3750
KAATELAENK RKIDALLDWV GLLWGHLRES HRLAFQEWSS SLELAMEKQT
3760 3770 3780 3790 3800
LAATDGHVDV NQVPETLDRQ YELMKARHQE LLSQQQNFIV ATQSVQSFLD
3810 3820 3830 3840 3850
QHSHNLTPEE RQKLQEKLGE LKEQYAASLA RSEAELKQTQ ALRDELQKFL
3860 3870 3880 3890 3900
QDHKEFENWL QQSENELDSM HKGGSSPEAL NSLLKRQGSF SEDVISHKGD
3910 3920 3930 3940 3950
LRFVTISGQK VLETENNFEE GQEPSATRNL VNEKLKDATE RYTTLHSKCI
3960 3970 3980 3990 4000
RLGSHLSMLL GQYQQFQSSA DSLQAWVLTC EASVGKLLSD TVASDPGVLQ
4010 4020 4030 4040 4050
QQLATTKQLQ EELAEHQVPV EKLQKAAHDL LDIEGEPALD CRPIQETTDS
4060 4070 4080 4090 4100
ISSRFQNLSC SLDERSALLQ KAIAQSQSVQ ESMESLLQSI REVEQNLERD
4110 4120 4130 4140 4150
QVASLSSGVI QEALANNMKL KQDIARQKSS LEATHDMVTR FMETADSNSA
4160 4170 4180 4190 4200
SVLQGKLAEL SQRFQQLQLQ QQEKESNLKK LLPQAEMFEQ LSNKLQQFME
4210 4220 4230 4240 4250
NKSRLLASGN QPDQDIAHFS QQIQELTLAM EDQKENLDTL EHLVTTLGSC
4260 4270 4280 4290 4300
GFALDLSQHQ DKIQNLKKDF TELQKTVQER EKDASTCQEQ LDEFRKLIRT
4310 4320 4330 4340 4350
FQKWLKETEG NVPPAKTFVS AKELEKQIEH LKDLISDWES KGALLGEINA
4360 4370 4380 4390 4400
KGTALESLIM DITAPDSQAK TGSILPPVGS SVGSVNGYHT CKDLTEIQCD
4410 4420 4430 4440 4450
MFDVNSKYEK LWEVLRERQE SLQTVFSRME EVQKEASSVL QWLESKEEVL
4460 4470 4480 4490 4500
KAMDATLSPT KTETVKAQAE SNKAFLAELE QNSPKIQKVK EALAGLLKTY
4510 4520 4530 4540 4550
PNSQEAENWK KMQEDLNSRW EKATEVTVAR QKQLEESASH LACFQAAESQ
4560 4570 4580 4590 4600
LRPWLMEKEL MMGVLGPLSI DPNMLKQQVQ FMLKEFEARR QQHEQLNEAA
4610 4620 4630 4640 4650
QGILTGPGDM SPSASQVHKD LQSISQKWVE LTDKLNSRSS QIDQAIVKST
4660 4670 4680 4690 4700
QYQDLLQDLS EKVKAIGQRL SGQSAISTQP EAVKQQLEET SEIRSDLGQL
4710 4720 4730 4740 4750
DNEIKEAQTL CQELSLLIGE QYLKDELKKR LETVALPLQG LEDLAADRMN
4760 4770 4780 4790 4800
RLQAALASTQ QFQQMFDELR TWLDEKQSQQ AKNCPISAKL ERLQCQLQEN
4810 4820 4830 4840 4850
EEFQKNLNQH SGSYEVIVAE GEALLLSVPP GEEKKTLQNQ LVELRSHWED
4860 4870 4880 4890 4900
LSKKTANRQS RLKDCMQKAQ KYQGHVEDLV PWIDECKSKM SELQVTLDPV
4910 4920 4930 4940 4950
QLESSLLRSK AMLNEAEKRR SLLEILNSAA DILINSSEID EDEIRDEKAG
4960 4970 4980 4990 5000
LNQNMDAITE ELQAKTSSLE EMTQRLKEFQ ESFKNIEKKV EGAKHQLEIF
5010 5020 5030 5040 5050
DALGSQACSN KNLEKLKAQQ EVLQALEPQV DYLRNFTQGL VEDAPDGSDA
5060 5070 5080 5090 5100
SPLVHQAEVA QQEFLEVKQR VSSSCLTMEN KLEGIGQFHC RVREMFSQLA
5110 5120 5130 5140 5150
DLDDELDGMG AIGRDTDSLQ SQIEDVRLFL NKIQALRFDI EDSEAECRKM
5160 5170 5180 5190 5200
LEEEGTLDLL GLKRELEALN KQCGKLTERG KVRQEQLELT LGRVEDFYRK
5210 5220 5230 5240 5250
LKALNDAATA AEEGEALQWI VGTEVDVINQ QLADFKLFQK DQVDPLQVKL
5260 5270 5280 5290 5300
QQVNGLGQGL IQSAGKNCDV QGLEHDMDEI NTRWNTLNKK VAQRIAQLQE
5310 5320 5330 5340 5350
ALLHCGKFQD ALEPLLSWLT DTEELIANQK PPSAEYKVVK AQIQEQKLLQ
5360 5370 5380 5390 5400
RLLDDRKATV DMLQAEGGRI AQSAELADRE KITGQLESLE RRWTDLLSKA
5410 5420 5430 5440 5450
AARQKQLEDI LVLAKQFHET AEPISDFLSV TANKLANSEP VGTQTAKIHQ
5460 5470 5480 5490 5500
QIIRHKALNE EIINRKKNVD QAIKNGQALL KQTTGEEVLL IQEKLDGIKT
5510 5520 5530 5540 5550
RYADITLTSS KALRTLEQAR QLATKFHSTY EELTGWLREA EEELAASGGQ
5560 5570 5580 5590 5600
SPTGEQIPQF QQRQKELKKE VMEHRLVLDT VNEVSHALLE LVPWRAREGL
5610 5620 5630 5640 5650
DKLVSDANEQ YKLISDTVGQ RVDEIDAAIQ RSQQYEQAAD AELAWVAETK
5660 5670 5680 5690 5700
RKLMALGPIR LEQDQTTAQL QVQKAFSIDI IRHKDSMDEL FSHRGEIFST
5710 5720 5730 5740 5750
CGEEQKAVLQ EKTECLIQQY EAVSLLNSER YARLERAQVL VNQFWETYEE
5760 5770 5780 5790 5800
LSPWAEETLA LIAQLPPPAV DHEQLRQQQE EMRQLRESIA EHKPHIDKIL
5810 5820 5830 5840 5850
KIGPQLKELN PEEGKMVEEK YQKAENMYAQ IKDEVRQRAL ALDEAVSQSA
5860 5870 5880 5890 5900
QFHDKIEPML ETLENLSSRL RMPPLIPAEV DKIRECISDN KSATVELEKL
5910 5920 5930 5940 5950
QPSFEALKRR GEELIGRSQG ADKDLAAKEI QDKLDQMVFF WEDIKARSEE
5960 5970 5980 5990 6000
REIKFLDVLE LAEKFWYDMA ALLTTIKDTQ DIVHDLESPG IDPSIIKQQV
6010 6020 6030 6040 6050
EAAETIKEET DGLHEELEFI RILGADLIFA CGETEKPEVK KSIDEMNNAW
6060 6070 6080 6090 6100
ENLNKTWKER LEKLEDAMQA AVQYQDTLQA MFDWLDNTVI KLCTMPPVGT
6110 6120 6130 6140 6150
DLNTVKDQLN EMKEFKVEVY QQQIEMEKLN HQGELMLKKA TDETDRDIIR
6160 6170 6180 6190 6200
EPLTELKHLW ENLGEKIAHR QHKLEGALLA LGQFQHALEE LMSWLTHTEE
6210 6220 6230 6240 6250
LLDAQRPISG DPKVIEVELA KHHVLKNDVL AHQATVATVN KAGSELLESS
6260 6270 6280 6290 6300
AGDDASSLRS RLETMNQCWE SVLQKTEERE QQLQSTLQQA QGFHSEIEDF
6310 6320 6330 6340 6350
LLELNRMENQ LSASKPTGGL PETAREQLDT HMELHSQLRA KEEIYNQLLD
6360 6370 6380 6390 6400
KGRLMLLSRG DSGSGSKTEQ SVALLEQKWH AVSSKVEERK SKLEEALSLA
6410 6420 6430 6440 6450
TEFQNSLQEF INWLTLAEQS LNIASPPSLI LNTVLSQIEE HKVFANEVND
6460 6470 6480 6490 6500
HRDQIIELDQ TGNQLKFLSQ KQDVVLIKNL LVSVQSRWEK VVQRSIERGR
6510 6520 6530 6540 6550
SLDDARKRAK QFHEAWKKLI DWLEDAESHL DSELEISNDP DKIKLQLSKH
6560 6570 6580 6590 6600
KEFQKTLGGK QPVYDTTIRT GRALKEKTLL AGDTQKLDNL LGEVRDKWDT
6610 6620 6630 6640 6650
VCGKSVERQH KLEEALLFSG QFMDALQALV DWLYKVEPQL AEDQPVHGDL
6660 6670 6680 6690 6700
DLVMNLMDAH KVFQKELGKR TGTVQVLKRS GRELIEGSRD DTTWVKGQLQ
6710 6720 6730 6740 6750
ELSTRWDTVC KLSVSKQSRL EQALKQAEEF RDTVHMLLEW LSEAEQTLRF
6760 6770 6780 6790 6800
RGALPDDTEA LQSLIDTHKE FMKKVEEKRV DVNTAVAMGE AILAVCHPDC
6810 6820 6830 6840 6850
ITTIKHWITI IRARFEEVLT WAKQHQQRLE TALSELVANA ELLEELLAWI
6860 6870 6880 6890 6900
QWAETTLIQR DQEPIPQNID RVKALITEHQ SFMEEMTRKQ PDVDRVTKTY
6910 6920 6930 6940 6950
KRKSVEPTHA PFMEKSRSGS RKSLNQPTPP PMPILSQSEA KNPRINQLSA
6960 6970 6980 6990 7000
RWQQVWLLAL ERQRKLNDAL DRLEELCPEL KEFANFDFDV WRKKYMRWMN
7010 7020 7030 7040 7050
HKKSRVMDFF RRIDKDQDGK ITRQEFIDGI LASKFPTTKL EMTAVADIFD
7060 7070 7080 7090 7100
RDGDGYIDYY EFVAALHPNK DAYRPTTDAD KIEDEVTRQV AQCKCAKRFQ
7110 7120 7130 7140 7150
VEQIGENKYR FFLGNQFGDS QQLRLVRILR STVMVRVGGG WMALDEFLVK
7160 7170 7180 7190 7200
NDPCRARGRT NIELREKFIL PEGASQGMTP FRSRGRRSKP SSRAASPTRS
7210 7220 7230 7240 7250
SSSASQSNHS CTSMPSSPAT PASGTKVISS SGSKLKRPTP AFHSSRTSLA
7260 7270 7280 7290 7300
GDTSNSSSPA STGAKANRAD PKKSASRPGS RAGSRAGSRA SSRRGSDASD
7310 7320 7330 7340 7350
FDLLETQSAC SDTSESSAAG GQGSSRRGLT KPSKIPTMSK KTTTASPRTP

GPKR
Length:7,354
Mass (Da):831,878
Last modified:June 28, 2011 - v2
Checksum:i2B68195989DE15C7
GO
Isoform 2 (identifier: Q9QXZ0-2) [UniParc]FASTAAdd to basket

Also known as: Macf1a

The sequence of this isoform differs from the canonical sequence as follows:
     1543-3569: Missing.

Show »
Length:5,327
Mass (Da):607,947
Checksum:i5E74E7C618CF0371
GO
Isoform 3 (identifier: Q9QXZ0-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-73: MSSSDEETLS...PAERAVVRVA → EKEFVQAYEDVLERYK
     1543-3569: Missing.

Note: Incomplete sequence.
Show »
Length:5,270
Mass (Da):601,744
Checksum:iB0135FD543CEAD3A
GO
Isoform 4 (identifier: Q9QXZ0-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-181: MSSSDEETLS...LIWTIILHFQ → MGNSLGCVKE...SEEDGTLSLE
     1543-3569: Missing.

Show »
Length:5,478
Mass (Da):622,158
Checksum:i1B7ECC46D414444A
GO

Sequence cautioni

The sequence AAC52989.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAM18553.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence CAM20969.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti242 – 2421D → N in AAD32244 (PubMed:10601340).Curated
Sequence conflicti242 – 2421D → N in AAC52988 (PubMed:8954775).Curated
Sequence conflicti242 – 2421D → N in AAC52989 (PubMed:8954775).Curated
Sequence conflicti242 – 2421D → N in AAC52990 (PubMed:8954775).Curated
Sequence conflicti393 – 3931P → L in AAD32244 (PubMed:10601340).Curated
Sequence conflicti393 – 3931P → L in AAY78553 (PubMed:16076900).Curated
Sequence conflicti393 – 3931P → L in AAC52988 (PubMed:8954775).Curated
Sequence conflicti393 – 3931P → L in AAC52989 (PubMed:8954775).Curated
Sequence conflicti794 – 7941D → N in AAY78553 (PubMed:16076900).Curated
Sequence conflicti815 – 8151C → A in AAD32244 (PubMed:10601340).Curated
Sequence conflicti815 – 8151C → A in AAC52988 (PubMed:8954775).Curated
Sequence conflicti815 – 8151C → A in AAC52989 (PubMed:8954775).Curated
Sequence conflicti815 – 8151C → A in AAC52990 (PubMed:8954775).Curated
Sequence conflicti928 – 9281E → G in AAD32244 (PubMed:10601340).Curated
Sequence conflicti928 – 9281E → G in AAY78553 (PubMed:16076900).Curated
Sequence conflicti3795 – 37951V → A in AAD32244 (PubMed:10601340).Curated
Sequence conflicti3795 – 37951V → A in AAY78553 (PubMed:16076900).Curated
Sequence conflicti3909 – 39091Q → H in AAC52988 (PubMed:8954775).Curated
Sequence conflicti3909 – 39091Q → H in AAC52989 (PubMed:8954775).Curated
Sequence conflicti3909 – 39091Q → H in AAC52990 (PubMed:8954775).Curated
Sequence conflicti4735 – 47351A → V in AAD32244 (PubMed:10601340).Curated
Sequence conflicti4735 – 47351A → V in AAY78553 (PubMed:16076900).Curated
Sequence conflicti4750 – 47501N → S in AAD32244 (PubMed:10601340).Curated
Sequence conflicti4750 – 47501N → S in AAY78553 (PubMed:16076900).Curated
Sequence conflicti4891 – 48911S → F in AAD32244 (PubMed:10601340).Curated
Sequence conflicti4891 – 48911S → F in AAY78553 (PubMed:16076900).Curated
Sequence conflicti5587 – 55871A → V in AAD32244 (PubMed:10601340).Curated
Sequence conflicti5587 – 55871A → V in AAY78553 (PubMed:16076900).Curated
Sequence conflicti5603 – 56031L → R in AAD32244 (PubMed:10601340).Curated
Sequence conflicti5603 – 56031L → R in AAY78553 (PubMed:16076900).Curated
Sequence conflicti5971 – 59711A → V in AAD32244 (PubMed:10601340).Curated
Sequence conflicti5971 – 59711A → V in AAY78553 (PubMed:16076900).Curated
Sequence conflicti6309 – 63091N → T in AAD32244 (PubMed:10601340).Curated
Sequence conflicti6309 – 63091N → T in AAY78553 (PubMed:16076900).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 181181MSSSD…ILHFQ → MGNSLGCVKEPKESIAVPEK APISPKKRVRFKRKWRGKKI LTPEASHREEALEGTGVIEE TETLTKLTARLPKEPGVGGA EHPPSDIFLPGDSAPNSGVG DQGMIVQVKESFQAEIQTAH LLLENESSVVGGAWDSLEEG MTVIAHLLDNPAERNCEKSV SQLVEFPRTASCSSRAVLLP LQGETAVEQGGTLLRHRHRS STLPRTDYPSETVDQDQPSE GWSVGGRTKSVPSAPPTGSW IAKCSVASSIPKQSGDPIHT EPTHVGLVSCKGPIMPASQS DLSVSGITVSILPSSSGYGS DGLRLHGIRPEDTEPEKTST PFSEEDGTLSLE in isoform 4. 1 PublicationVSP_041394Add
BLAST
Alternative sequencei1 – 7373MSSSD…VVRVA → EKEFVQAYEDVLERYK in isoform 3. CuratedVSP_041395Add
BLAST
Alternative sequencei1543 – 35692027Missing in isoform 2, isoform 3 and isoform 4. 2 PublicationsVSP_041396Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF150755 mRNA. Translation: AAD32244.1.
DQ067088 mRNA. Translation: AAY78553.1.
AL606932, AL606918 Genomic DNA. Translation: CAM18553.1. Sequence problems.
AL606918, AL606932 Genomic DNA. Translation: CAM20969.1. Sequence problems.
U67203 mRNA. Translation: AAC52988.1.
U67204 mRNA. Translation: AAC52989.1. Different initiation.
U67205 mRNA. Translation: AAC52990.1.
PIRiT30847.
T30849.
T42725.
UniGeneiMm.402299.
Mm.485351.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF150755 mRNA. Translation: AAD32244.1.
DQ067088 mRNA. Translation: AAY78553.1.
AL606932, AL606918 Genomic DNA. Translation: CAM18553.1. Sequence problems.
AL606918, AL606932 Genomic DNA. Translation: CAM20969.1. Sequence problems.
U67203 mRNA. Translation: AAC52988.1.
U67204 mRNA. Translation: AAC52989.1. Different initiation.
U67205 mRNA. Translation: AAC52990.1.
PIRiT30847.
T30849.
T42725.
UniGeneiMm.402299.
Mm.485351.

3D structure databases

ProteinModelPortaliQ9QXZ0.
SMRiQ9QXZ0. Positions 74-298.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QXZ0. 9 interactions.
MINTiMINT-1634787.
STRINGi10090.ENSMUSP00000095507.

PTM databases

iPTMnetiQ9QXZ0.
PhosphoSiteiQ9QXZ0.

Proteomic databases

EPDiQ9QXZ0.
MaxQBiQ9QXZ0.
PaxDbiQ9QXZ0.
PRIDEiQ9QXZ0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:108559. Macf1.

Phylogenomic databases

eggNOGiKOG0516. Eukaryota.
COG5069. LUCA.
HOGENOMiHOG000049054.
HOVERGENiHBG031127.
InParanoidiQ9QXZ0.

Miscellaneous databases

ChiTaRSiMacf1. mouse.
NextBioi278682.
PROiQ9QXZ0.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QXZ0.
CleanExiMM_MACF1.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.418.10. 2 hits.
3.30.920.20. 1 hit.
3.90.1290.10. 5 hits.
InterProiIPR001589. Actinin_actin-bd_CS.
IPR001715. CH-domain.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR003108. GAS2_dom.
IPR028408. Macf1.
IPR001101. Plectin_repeat.
IPR018159. Spectrin/alpha-actinin.
IPR002017. Spectrin_repeat.
[Graphical view]
PANTHERiPTHR11915:SF37. PTHR11915:SF37. 6 hits.
PfamiPF00307. CH. 2 hits.
PF13499. EF-hand_7. 1 hit.
PF02187. GAS2. 1 hit.
PF00681. Plectin. 8 hits.
PF00435. Spectrin. 18 hits.
[Graphical view]
SMARTiSM00033. CH. 2 hits.
SM00054. EFh. 2 hits.
SM00243. GAS2. 1 hit.
SM00250. PLEC. 19 hits.
SM00150. SPEC. 34 hits.
[Graphical view]
SUPFAMiSSF143575. SSF143575. 1 hit.
SSF47473. SSF47473. 1 hit.
SSF47576. SSF47576. 1 hit.
SSF75399. SSF75399. 6 hits.
PROSITEiPS00019. ACTININ_1. 1 hit.
PS00020. ACTININ_2. 1 hit.
PS50021. CH. 2 hits.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS51460. GAR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Microtubule actin cross-linking factor (MACF): a hybrid of dystonin and dystrophin that can interact with the actin and microtubule cytoskeletons."
    Leung C.L., Sun D., Zheng M., Knowles D.R., Liem R.K.H.
    J. Cell Biol. 147:1275-1286(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: BALB/cJ.
  2. "Microtubule actin crosslinking factor 1b: a novel plakin that localizes to the Golgi complex."
    Lin C.-M., Chen H.-J., Leung C.L., Parry D.A.D., Liem R.K.H.
    J. Cell Sci. 118:3727-3738(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: BALB/cJ.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "Cloning and characterization of mouse ACF7, a novel member of the dystonin subfamily of actin binding proteins."
    Bernier G., Mathieu M., De Repentigny Y., Vidal S.M., Kothary R.
    Genomics 38:19-29(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-3909 (ISOFORMS 2 AND 4), PARTIAL NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: BALB/cJ.
    Tissue: Brain.
  5. Lubec G., Kang S.U.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 881-888; 5824-5832 AND 6671-6678, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: C57BL/6J.
    Tissue: Brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  7. "The role of microtubule actin cross-linking factor 1 (MACF1) in the Wnt signaling pathway."
    Chen H.J., Lin C.M., Lin C.S., Perez-Olle R., Leung C.L., Liem R.K.
    Genes Dev. 20:1933-1945(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  8. "Comprehensive identification of phosphorylation sites in postsynaptic density preparations."
    Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L.
    Mol. Cell. Proteomics 5:914-922(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-280, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  9. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
    Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
    Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain cortex.
  10. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-3082; SER-3085; SER-7296 AND SER-7299, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  11. "ACF7 regulates cytoskeletal-focal adhesion dynamics and migration and has ATPase activity."
    Wu X., Kodama A., Fuchs E.
    Cell 135:137-148(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH MAPRE1; CLASP1 AND CLASP2.
  12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35; SER-57; SER-280; SER-1376; SER-2077; SER-2081; SER-3082; SER-3085; SER-3889; SER-4458; SER-4483; THR-5394; SER-5988; SER-6923; SER-7296 AND SER-7299, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-42 (ISOFORM 4), IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  13. "Skin stem cells orchestrate directional migration by regulating microtubule-ACF7 connections through GSK3beta."
    Wu X., Shen Q.T., Oristian D.S., Lu C.P., Zheng Q., Wang H.W., Fuchs E.
    Cell 144:341-352(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiMACF1_MOUSE
AccessioniPrimary (citable) accession number: Q9QXZ0
Secondary accession number(s): B1ARU3
, P97394, P97395, P97396, Q4PLL5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 28, 2011
Last modified: May 11, 2016
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.