ID   PEX3_MOUSE              Reviewed;         372 AA.
AC   Q9QXY9;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   24-JAN-2024, entry version 156.
DE   RecName: Full=Peroxisomal biogenesis factor 3;
DE   AltName: Full=Peroxin-3;
DE   AltName: Full=Peroxisomal assembly protein PEX3;
GN   Name=Pex3;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC   STRAIN=129/Ola;
RX   PubMed=10839463; DOI=10.1515/bc.2000.044;
RA   Muntau A.C., Mayerhofer P.U., Albet S., Schmid T.E., Bugaut M.,
RA   Roscher A.A., Kammerer S.;
RT   "Genomic organization, expression analysis, and chromosomal localization of
RT   the mouse PEX3 gene encoding a peroxisomal assembly protein.";
RL   Biol. Chem. 381:337-342(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Mammary gland, and Retina;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Kidney, Liver, Lung, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Involved in peroxisome biosynthesis and integrity. Assembles
CC       membrane vesicles before the matrix proteins are translocated. As a
CC       docking factor for PEX19, is necessary for the import of peroxisomal
CC       membrane proteins in the peroxisomes. {ECO:0000250|UniProtKB:P56589}.
CC   -!- SUBUNIT: Interacts with PEX19. {ECO:0000250|UniProtKB:P56589}.
CC   -!- SUBCELLULAR LOCATION: Peroxisome membrane
CC       {ECO:0000250|UniProtKB:P56589}; Multi-pass membrane protein
CC       {ECO:0000255}.
CC   -!- TISSUE SPECIFICITY: Identified in all tissues analyzed, with the
CC       strongest expression in liver and in testis.
CC   -!- SIMILARITY: Belongs to the peroxin-3 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF152996; AAF14524.1; -; mRNA.
DR   EMBL; AF162896; AAG24507.1; -; Genomic_DNA.
DR   EMBL; AF162890; AAG24507.1; JOINED; Genomic_DNA.
DR   EMBL; AF162891; AAG24507.1; JOINED; Genomic_DNA.
DR   EMBL; AF162892; AAG24507.1; JOINED; Genomic_DNA.
DR   EMBL; AF162893; AAG24507.1; JOINED; Genomic_DNA.
DR   EMBL; AF162894; AAG24507.1; JOINED; Genomic_DNA.
DR   EMBL; AF162895; AAG24507.1; JOINED; Genomic_DNA.
DR   EMBL; BC033415; AAH33415.1; -; mRNA.
DR   EMBL; BC037606; AAH37606.1; -; mRNA.
DR   CCDS; CCDS23702.1; -.
DR   RefSeq; NP_064345.1; NM_019961.3.
DR   AlphaFoldDB; Q9QXY9; -.
DR   SMR; Q9QXY9; -.
DR   BioGRID; 208045; 1.
DR   STRING; 10090.ENSMUSP00000019945; -.
DR   iPTMnet; Q9QXY9; -.
DR   PhosphoSitePlus; Q9QXY9; -.
DR   SwissPalm; Q9QXY9; -.
DR   EPD; Q9QXY9; -.
DR   jPOST; Q9QXY9; -.
DR   MaxQB; Q9QXY9; -.
DR   PaxDb; 10090-ENSMUSP00000019945; -.
DR   ProteomicsDB; 301794; -.
DR   Pumba; Q9QXY9; -.
DR   Antibodypedia; 33142; 148 antibodies from 25 providers.
DR   DNASU; 56535; -.
DR   Ensembl; ENSMUST00000019945.15; ENSMUSP00000019945.9; ENSMUSG00000019809.17.
DR   GeneID; 56535; -.
DR   KEGG; mmu:56535; -.
DR   UCSC; uc007eky.2; mouse.
DR   AGR; MGI:1929646; -.
DR   CTD; 8504; -.
DR   MGI; MGI:1929646; Pex3.
DR   VEuPathDB; HostDB:ENSMUSG00000019809; -.
DR   eggNOG; KOG4444; Eukaryota.
DR   GeneTree; ENSGT00390000008481; -.
DR   HOGENOM; CLU_041367_2_0_1; -.
DR   InParanoid; Q9QXY9; -.
DR   OMA; HRGWKDL; -.
DR   OrthoDB; 5477008at2759; -.
DR   PhylomeDB; Q9QXY9; -.
DR   TreeFam; TF352826; -.
DR   Reactome; R-MMU-1369062; ABC transporters in lipid homeostasis.
DR   Reactome; R-MMU-9603798; Class I peroxisomal membrane protein import.
DR   BioGRID-ORCS; 56535; 11 hits in 78 CRISPR screens.
DR   ChiTaRS; Pex3; mouse.
DR   PRO; PR:Q9QXY9; -.
DR   Proteomes; UP000000589; Chromosome 10.
DR   RNAct; Q9QXY9; Protein.
DR   Bgee; ENSMUSG00000019809; Expressed in spermatid and 251 other cell types or tissues.
DR   ExpressionAtlas; Q9QXY9; baseline and differential.
DR   GO; GO:0005829; C:cytosol; ISO:MGI.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005778; C:peroxisomal membrane; ISO:MGI.
DR   GO; GO:0005777; C:peroxisome; ISO:MGI.
DR   GO; GO:0005886; C:plasma membrane; ISS:MGI.
DR   GO; GO:0032991; C:protein-containing complex; ISO:MGI.
DR   GO; GO:0032994; C:protein-lipid complex; ISO:MGI.
DR   GO; GO:0008289; F:lipid binding; ISO:MGI.
DR   GO; GO:0030674; F:protein-macromolecule adaptor activity; IBA:GO_Central.
DR   GO; GO:0016557; P:peroxisome membrane biogenesis; ISO:MGI.
DR   GO; GO:0007031; P:peroxisome organization; ISO:MGI.
DR   GO; GO:0045046; P:protein import into peroxisome membrane; ISO:MGI.
DR   InterPro; IPR006966; Peroxin-3.
DR   PANTHER; PTHR28080; PEROXISOMAL BIOGENESIS FACTOR 3; 1.
DR   PANTHER; PTHR28080:SF1; PEROXISOMAL BIOGENESIS FACTOR 3; 1.
DR   Pfam; PF04882; Peroxin-3; 3.
DR   Genevisible; Q9QXY9; MM.
PE   1: Evidence at protein level;
KW   Membrane; Peroxisome; Peroxisome biogenesis; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..372
FT                   /note="Peroxisomal biogenesis factor 3"
FT                   /id="PRO_0000208739"
FT   TOPO_DOM        1..15
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        37..116
FT                   /note="Peroxisomal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        117..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        141..372
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          1..45
FT                   /note="Targeting to peroxisomes"
FT                   /evidence="ECO:0000250|UniProtKB:P56589"
FT   REGION          120..136
FT                   /note="Interaction with PEX19"
FT                   /evidence="ECO:0000250|UniProtKB:P56589"
SQ   SEQUENCE   372 AA;  42224 MW;  77AA9590A53FAB77 CRC64;
     MLRSMWNFLK RHKKKCIFLG TVLGGVYILG KYGQKKIREI QEREAAEYIA QARRQYHFES
     NQRTCNMTVL SMLPTLREAL MQQLNSESLT ALLKSRPSNK LEIWEDLKII SFTRSIVAVY
     STCMLVVLLR VQLNIIGGYI YLDNATVGKN GTTVLAPPDV QQQYLSSIQH LLGDGLTELV
     TVIKQAVQRI LGSVSLKHSL SLLDLEQKLK EIRILVEQHQ SSWNDKDVSR SSLCQYMMPD
     EETPLAAQAY GLSHRDITTI KLLNETRDML ESPDFSTVLN TCLNRGFSRL LDNMAEFFRP
     TEQDLQHGNS INSLSSVSLP LAKIIPIVNG QIHSVCSETP SHFVQDLLMM EQVKDFAANV
     YEAFSTPQQL EK
//