Q9QXY2 (SRCN1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SRC kinase signaling inhibitor 1

Alternative name(s):
SNAP-25-interacting protein
Short name=SNIP
p130Cas-associated protein
p140Cap

Gene names

Name:	Srcin1
Synonyms:	P140, Snip

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	1197 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a negative regulator of SRC by activating CSK which inhibits SRC activity and downstream signaling, leading to impaired cell spreading and migration. Regulates dendritic spine morphology. Involved in calcium-dependent exocytosis. May play a role in neurotransmitter release or synapse maintenance. Ref.1 Ref.3
Subunit structure	Interacts with BCAR1/p130Cas through its C-terminal domain and with CSK, CTTN and SRC By similarity. Also interacts with MAPRE3/EB3, SORBS3/vinexin and the N-terminal coiled-coil region of SNAP25. Ref.1 Ref.2 Ref.3 UniProtKB Q9C0H9
Subcellular location	Cytoplasm. Cytoplasm › cytoskeleton. Cell projection › axon. Cell projection › dendrite. Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Note: Localized to the perinuclear region, lamellopodia, cortical actin and actin stress fibers but not to focal adhesions. Strongly expressed in axons and dendrites of the CA1 and CA3 hippocampal regions and of the dentate gyrus. Detected in both pre- and post-synapses and in post-synaptic density fractions. Ref.1 Ref.2 Ref.3
Tissue specificity	Expressed exclusively in brain. Abundant in telencephalon and expressed moderately in cerebellum, hypothalamus, thalamus, superior and inferior colliculi, and olfactory bulb. No expression detected in medulla oblongata, spinal cord or pituitary gland. Enriched in the neuropil rather than soma in the thalamus, corpus striatum and cerebral cortex. Detected in astrocytes. Ref.1 Ref.2
Developmental stage	In the embryo, expression increases dramatically between E14.5 and E18.5 (at protein level). Ref.2
Post-translational modification	Tyrosine-phosphorylated in response to EGF and to cell adhesion to integrin ligands By similarity. UniProtKB Q9C0H9
Sequence similarities	Belongs to the SRCIN1 family.

Ontologies

Keywords
Biological process	Exocytosis
Cellular component	Cell junction Cell membrane Cell projection Cytoplasm Cytoskeleton Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	exocytosis Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of protein secretion Inferred from mutant phenotype Ref.1. Source: RGD negative regulation of protein tyrosine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein tyrosine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell migration Inferred from sequence or structural similarity. Source: UniProtKB regulation of dendritic spine morphogenesis Inferred from sequence or structural similarity. Source: UniProtKB substrate adhesion-dependent cell spreading Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	actin cytoskeleton Inferred from direct assay Ref.1. Source: RGD axon Inferred from direct assay Ref.2. Source: UniProtKB cell junction Inferred from electronic annotation. Source: UniProtKB-KW cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB filopodium Inferred from direct assay Ref.1. Source: RGD lamellipodium Inferred from direct assay Ref.1. Source: RGD neuronal cell body Inferred from direct assay Ref.1. Source: RGD plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic density Inferred from direct assay Ref.3. Source: UniProtKB postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW synaptosome Inferred from direct assay Ref.1. Source: RGD
Molecular function	protein domain specific binding Inferred from direct assay Ref.1. Source: RGD protein kinase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Snap25	P60881	3	EBI-1394088,EBI-1027214

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q9QXY2-1)

Also known as: SNIP-b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: Q9QXY2-2)

Also known as: SNIP-a;

The sequence of this isoform differs from the canonical sequence as follows:
     1174-1197: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1197	1197	SRC kinase signaling inhibitor 1		PRO_0000072013
Regions
	Region	681 – 731	51	Interaction with SNAP25
	Coiled coil	594 – 621	28	Potential
	Coiled coil	685 – 708	24	Potential
	Coiled coil	750 – 812	63	Potential
	Compositional bias	990 – 1048	59	Pro-rich
Amino acid modifications
	Modified residue	79	1	Phosphoserine By similarity
	Modified residue	98	1	Phosphoserine By similarity
	Modified residue	175	1	Phosphoserine By similarity
	Modified residue	200	1	Phosphoserine By similarity
	Modified residue	260	1	Phosphoserine By similarity
	Modified residue	276	1	Phosphotyrosine By similarity
	Modified residue	342	1	Phosphoserine By similarity
	Modified residue	378	1	Phosphoserine By similarity
	Modified residue	397	1	Phosphoserine By similarity
	Modified residue	431	1	Phosphotyrosine By similarity
	Modified residue	547	1	Phosphoserine By similarity
	Modified residue	556	1	Phosphoserine By similarity
	Modified residue	637	1	Phosphoserine By similarity
	Modified residue	671	1	Phosphothreonine By similarity
	Modified residue	900	1	Phosphoserine By similarity
	Modified residue	941	1	Phosphoserine By similarity
	Modified residue	1021	1	Phosphoserine By similarity
	Modified residue	1077	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	1174 – 1197	24	Missing in isoform 2. Ref.1		VSP_050633

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (SNIP-b) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 8C9A2E630FB31C33	FASTA	1,197	129,744
	10 20 30 40 50 60 MQPWQCLRRF ALAWWERTAE GRARSPREEV GPRDPGGRGE PDPERSSPPM LSADDAEYPR 70 80 90 100 110 120 EYRTLGGGGS GGSGGRRFSN VGLVHTSERR HTVIAAQSLE ALSGLQKADA DRKRDAFMDH 130 140 150 160 170 180 LKSKYPQHAL ALRGQQDRMR EQQPNYWSFK TRSSRHTQGA QPGLADQAAK LSYASAESLE 190 200 210 220 230 240 TMSEAELPLG FSRMNRFRQS LPLSRSASQT KLRSPGVLFL QFGEETRRVH ITHEVSSLDT 250 260 270 280 290 300 LHALIAHMFP QKLTMGMLKS PNTAILIKDE ARNVFYELED VRDIQDRSII KIYRKEPLYA 310 320 330 340 350 360 AFPGSHLTNG DLRREMVYAS RESSPTRRLN NLSPASHLAS SSPPPGLPSG LPSGLPSGSP 370 380 390 400 410 420 SRSRLSYAGG RPPSYAGSPV HHAAERLGGA PTSQGVSPSP SAILERRDVK PDEDLAGKAG 430 440 450 460 470 480 GMVLVKGEGL YADPYGLLHE GRLSLAAAAG DPFAYPGAGG LYKRGSVRSL STYSAAALQS 490 500 510 520 530 540 DLEDSLYKAG AGGPLYGDGY GFRLPPSSPQ KLADVSAPSG GPPPPHSPYS GPPSRGSPVR 550 560 570 580 590 600 QSFRKDSGSS SVFAESPGGK ARSTGASTAG APPSELFPGP GERSLVGFGP PVPAKDTETR 610 620 630 640 650 660 ERMEAMEKQI ASLTGLVQSA LLRGSEPETP SEKIEGSNGA ATPSAPVCGS GSRSSGATPV 670 680 690 700 710 720 SGPPPPAVSS TPAGQPTAVS RLQMQMHLRG LQNSASDLRG QLQQLRKLQL QNQESVRALL 730 740 750 760 770 780 KRTEAELSMR VSEAARRQED PLQRQRTLVE EERLRYLNDE ELITQQLNDL EKSVEKIQRD 790 800 810 820 830 840 VAHNHRLVPG PELEEKALVL KQLGETLTEL KAHFPGLQSK MRVVLRVEVE AVKFLKEEPQ 850 860 870 880 890 900 RLDGLLKRCR VVTDTLAQIR RQVDEGVWPP PNNLLNQSPK KVAAETDFSK GLDFEIPPPS 910 920 930 940 950 960 PPLNLHELSG PAEGTPLTPK SGNPTKGLDA PSKRNMDKAV SVEAAERDWE EKRAALTQYS 970 980 990 1000 1010 1020 AKDINRLLEE TQAELLKAIP DLDCASKTHP GPTPTPDHKP PKAPHGQKAA PRTEPSGRRG 1030 1040 1050 1060 1070 1080 SDELTVPRYR TEKPSKSPPP PPPRRSFPSS HGLTTTRTGE VVVTSKKDSV FIKKAESEEL 1090 1100 1110 1120 1130 1140 EIQKPQVKLR RAVSEVVRPA STPPIMASAI KDEDDEERII AELESGGGSV PPMKVVTPGA 1150 1160 1170 1180 1190 SRLKAAQGPA GSPDKGKHGK QRTEYMRIQA QQQVRVGYQA PRPLEGCTPR LCYMPLS
	Isoform 2 (SNIP-a) [UniParc]. Checksum: B6DF0EEC27B69080	FASTA	1,173	127,055
	10 20 30 40 50 60 MQPWQCLRRF ALAWWERTAE GRARSPREEV GPRDPGGRGE PDPERSSPPM LSADDAEYPR 70 80 90 100 110 120 EYRTLGGGGS GGSGGRRFSN VGLVHTSERR HTVIAAQSLE ALSGLQKADA DRKRDAFMDH 130 140 150 160 170 180 LKSKYPQHAL ALRGQQDRMR EQQPNYWSFK TRSSRHTQGA QPGLADQAAK LSYASAESLE 190 200 210 220 230 240 TMSEAELPLG FSRMNRFRQS LPLSRSASQT KLRSPGVLFL QFGEETRRVH ITHEVSSLDT 250 260 270 280 290 300 LHALIAHMFP QKLTMGMLKS PNTAILIKDE ARNVFYELED VRDIQDRSII KIYRKEPLYA 310 320 330 340 350 360 AFPGSHLTNG DLRREMVYAS RESSPTRRLN NLSPASHLAS SSPPPGLPSG LPSGLPSGSP 370 380 390 400 410 420 SRSRLSYAGG RPPSYAGSPV HHAAERLGGA PTSQGVSPSP SAILERRDVK PDEDLAGKAG 430 440 450 460 470 480 GMVLVKGEGL YADPYGLLHE GRLSLAAAAG DPFAYPGAGG LYKRGSVRSL STYSAAALQS 490 500 510 520 530 540 DLEDSLYKAG AGGPLYGDGY GFRLPPSSPQ KLADVSAPSG GPPPPHSPYS GPPSRGSPVR 550 560 570 580 590 600 QSFRKDSGSS SVFAESPGGK ARSTGASTAG APPSELFPGP GERSLVGFGP PVPAKDTETR 610 620 630 640 650 660 ERMEAMEKQI ASLTGLVQSA LLRGSEPETP SEKIEGSNGA ATPSAPVCGS GSRSSGATPV 670 680 690 700 710 720 SGPPPPAVSS TPAGQPTAVS RLQMQMHLRG LQNSASDLRG QLQQLRKLQL QNQESVRALL 730 740 750 760 770 780 KRTEAELSMR VSEAARRQED PLQRQRTLVE EERLRYLNDE ELITQQLNDL EKSVEKIQRD 790 800 810 820 830 840 VAHNHRLVPG PELEEKALVL KQLGETLTEL KAHFPGLQSK MRVVLRVEVE AVKFLKEEPQ 850 860 870 880 890 900 RLDGLLKRCR VVTDTLAQIR RQVDEGVWPP PNNLLNQSPK KVAAETDFSK GLDFEIPPPS 910 920 930 940 950 960 PPLNLHELSG PAEGTPLTPK SGNPTKGLDA PSKRNMDKAV SVEAAERDWE EKRAALTQYS 970 980 990 1000 1010 1020 AKDINRLLEE TQAELLKAIP DLDCASKTHP GPTPTPDHKP PKAPHGQKAA PRTEPSGRRG 1030 1040 1050 1060 1070 1080 SDELTVPRYR TEKPSKSPPP PPPRRSFPSS HGLTTTRTGE VVVTSKKDSV FIKKAESEEL 1090 1100 1110 1120 1130 1140 EIQKPQVKLR RAVSEVVRPA STPPIMASAI KDEDDEERII AELESGGGSV PPMKVVTPGA 1150 1160 1170 SRLKAAQGPA GSPDKGKHGK QRTEYMRIQA QQQ

References

[1]	"SNIP, a novel SNAP-25-interacting protein implicated in regulated exocytosis." Chin L.-S., Nugent R.D., Raynor M.C., Vavalle J.P., Li L. J. Biol. Chem. 275:1191-1200(2000) [PubMed: 10625663] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH SNAP25.
[2]	"Characterization of a multidomain adaptor protein, p140Cap, as part of a pre-synaptic complex." Ito H., Atsuzawa K., Sudo K., Di Stefano P., Iwamoto I., Morishita R., Takei S., Semba R., Defilippi P., Asano T., Usuda N., Nagata K. J. Neurochem. 107:61-72(2008) [PubMed: 18662323] [Abstract] Cited for: INTERACTION WITH SORBS3, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"Dynamic microtubules regulate dendritic spine morphology and synaptic plasticity." Jaworski J., Kapitein L.C., Gouveia S.M., Dortland B.R., Wulf P.S., Grigoriev I., Camera P., Spangler S.A., Di Stefano P., Demmers J., Krugers H., Defilippi P., Akhmanova A., Hoogenraad C.C. Neuron 61:85-100(2009) [PubMed: 19146815] [Abstract] Cited for: FUNCTION, INTERACTION WITH MAPRE3, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF156981 mRNA. Translation: AAF25003.1. AF156982 mRNA. Translation: AAF25004.1.
IPI	IPI00213633. IPI00400480.
RefSeq	NP_062251.1. NM_019378.1.
UniGene	Rn.207205.
3D structure databases
ProteinModelPortal	Q9QXY2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9QXY2. 4 interactions.
MINT	MINT-139008.
STRING	Q9QXY2.
PTM databases
PhosphoSite	Q9QXY2.
Proteomic databases
PRIDE	Q9QXY2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000016070; ENSRNOP00000016070; ENSRNOG00000011475.
GeneID	56029.
KEGG	rno:56029.
UCSC	AF156981. rat. NM_019378. rat.
Organism-specific databases
CTD	80725.
RGD	708439. Srcin1.
Phylogenomic databases
eggNOG	roNOG12795.
GeneTree	ENSGT00390000012399.
HOVERGEN	HBG019587.
Gene expression databases
ArrayExpress	Q9QXY2.
Genevestigator	Q9QXY2.
GermOnline	ENSRNOG00000011475. Rattus norvegicus.
Family and domain databases
ProtoNet	Search...
Other
NextBio	611083.

Entry information

Entry name

SRCN1_RAT

Accession

Primary (citable) accession number: Q9QXY2
Secondary accession number(s): Q9QXY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program