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Protein

Tight junction protein ZO-3

Gene

Tjp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tjp1, Tjp2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:17000770). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Binds and recruits PatJ to tight junctions where it connects and stabilizes apical and lateral components of tight junctions (By similarity). Promotes cell-cycle progression through the sequestration of cyclin D1 (Ccnd1) at tight junctions during mitosis which prevents Ccnd1 degradation during M-phase and enables S-phase transition (PubMed:21411630). With Tjp1 and Tjp2, participates to the junctional retention and stability of the transcription factor DbpA, but is not involved in its shuttling to the nucleus (By similarity). Contrary to Tjp2, Tjp3 is dispensable for individual viability, embryonic development, epithelial differentiation, and the establishment of TJs, at least in the laboratory environment (PubMed:17000770, PubMed:18172007).By similarity3 Publications

GO - Biological processi

  • regulation of G1/S transition of mitotic cell cycle Source: MGI

Names & Taxonomyi

Protein namesi
Recommended name:
Tight junction protein ZO-3
Alternative name(s):
Tight junction protein 3
Zona occludens protein 3
Zonula occludens protein 3
Gene namesi
Name:Tjp3
Synonyms:Zo3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1351650 Tjp3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Nucleus, Tight junction

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000945471 – 905Tight junction protein ZO-3Add BLAST905

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei111PhosphoserineBy similarity1
Modified residuei128PhosphoserineBy similarity1
Modified residuei156PhosphoserineCombined sources1
Modified residuei157PhosphoserineCombined sources1
Modified residuei161PhosphoserineCombined sources1
Modified residuei195PhosphoserineBy similarity1
Modified residuei311PhosphoserineCombined sources1
Modified residuei317PhosphothreonineBy similarity1
Modified residuei319PhosphoserineBy similarity1
Modified residuei343PhosphoserineCombined sources1
Modified residuei359PhosphoserineBy similarity1
Modified residuei584PhosphoserineBy similarity1
Modified residuei891PhosphoserineCombined sources1
Modified residuei892PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9QXY1
PaxDbiQ9QXY1
PRIDEiQ9QXY1

PTM databases

iPTMnetiQ9QXY1
PhosphoSitePlusiQ9QXY1

Expressioni

Tissue specificityi

Is concentrated in various types of epithelium, in tissues such as the lung, liver and kidney, but not in endothelium or at cadherin-based cell-cell adhesion sites (PubMed:14622136).1 Publication

Gene expression databases

CleanExiMM_TJP3

Interactioni

Subunit structurei

Interacts with occludin OCLN, claudins and TPJ1 (By similarity). Interacts with PATJ (By similarity). Interacts with UBN1 (By similarity). Interacts with FASLG (By similarity). Interacts with CCND1 (PubMed:21411630).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Cldn1O885513EBI-7158997,EBI-7158428

Protein-protein interaction databases

IntActiQ9QXY1, 1 interactor
MINTiQ9QXY1
STRINGi10090.ENSMUSP00000036438

Structurei

3D structure databases

ProteinModelPortaliQ9QXY1
SMRiQ9QXY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 93PDZ 1PROSITE-ProRule annotationAdd BLAST83
Domaini187 – 264PDZ 2PROSITE-ProRule annotationAdd BLAST78
Domaini368 – 434PDZ 3PROSITE-ProRule annotationAdd BLAST67
Domaini464 – 541SH3PROSITE-ProRule annotationAdd BLAST78
Domaini573 – 754Guanylate kinase-likePROSITE-ProRule annotationAdd BLAST182

Sequence similaritiesi

Belongs to the MAGUK family.Curated

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiKOG3580 Eukaryota
ENOG410XQP3 LUCA
HOGENOMiHOG000230923
HOVERGENiHBG017627
InParanoidiQ9QXY1
PhylomeDBiQ9QXY1

Family and domain databases

InterProiView protein in InterPro
IPR008145 GK/Ca_channel_bsu
IPR008144 Guanylate_kin-like_dom
IPR027417 P-loop_NTPase
IPR001478 PDZ
IPR036034 PDZ_sf
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
IPR005417 ZO
IPR005420 ZO-3
PANTHERiPTHR13865:SF11 PTHR13865:SF11, 1 hit
PfamiView protein in Pfam
PF00625 Guanylate_kin, 1 hit
PF00595 PDZ, 3 hits
PF07653 SH3_2, 1 hit
PRINTSiPR01597 ZONOCCLUDNS
PR01600 ZONOCCLUDNS3
SMARTiView protein in SMART
SM00072 GuKc, 1 hit
SM00228 PDZ, 3 hits
SUPFAMiSSF50044 SSF50044, 1 hit
SSF50156 SSF50156, 3 hits
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50052 GUANYLATE_KINASE_2, 1 hit
PS50106 PDZ, 3 hits
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9QXY1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELTIWEQH TATLYKDPRR GFGIAVSGGH DRASGSVVVS DVVPGSPAEG
60 70 80 90 100
RLRTGDHIVM VNGVSVENVT SAFAIQILKT CTKTANVTVK RPRRVQLPAT
110 120 130 140 150
KASPASGHQL SDQEEADHGR GYEGDSSSGS GRSWGERSRR SRAGRRGRVG
160 170 180 190 200
SHGRRSSGGG SEANGLDLVS GYKRLPKQDV LMRPLKSVLV KRRNSEEFGV
210 220 230 240 250
KLGSQIFIKH ITESGLAARN HGLQEGDLIL QINGVSSANL SLSDTRRLIE
260 270 280 290 300
KSEGELTLLV LRDSGQFLVN IPPAVSDSDS SLMEDISDLT SELSQAPPSH
310 320 330 340 350
VPPPPLKGQR SPEDSQTDSP VETPQPRRRE RSVNSRAIAE PESPGESRYD
360 370 380 390 400
IYRVPSRQSL EDRGYSPDTR VVSFPKGASI GLRLAGGNDV GIFVSGVQAG
410 420 430 440 450
SPADGQGIQE GDEILQVNGM PFRNLTREEA VQFLLGLPPG EDMELVTQSK
460 470 480 490 500
TGHSLRRWSQ SRVGDSFYIR THFELEPSPP YGLGFTRGDV FHVVDTLYPG
510 520 530 540 550
SGPGHGHSSH GGLWLAARMG RDLREQERGV IPNQSRAEQL ASLEAAQRAA
560 570 580 590 600
GVGPGASVGS NPRAEFWRLR SLRRGTKKAS TQRSREDLSA LTRQGHYPPY
610 620 630 640 650
ERVVLREASF KRPVVILGPV ADIAMKKLTT EMPEEFEIAE SMSRTDSPSK
660 670 680 690 700
IIKLDTVRVI AERDKHALLD VTPSAIERLN YVQYYPIVIF CAPESRPALK
710 720 730 740 750
ALREWLAPAS RRSSRRLYAQ AQKLQKHSGH LFTATIPLHG TSDSWYQEVK
760 770 780 790 800
AVIQQQQARP IWTAEDQLNS SSEDLDLTGH GLAASSGDLS CDSRTNSDYE
810 820 830 840 850
DTDGEGAYTD REGGPQDVDE EVAPTALARS SEPVWVDDHQ GLMGHGTTIT
860 870 880 890 900
DKWETQADSH YTQDQRRQDS MRTYKHEALR KKFTRARDVE SSDDEGYDWG

PATDL
Length:905
Mass (Da):99,324
Last modified:May 1, 2000 - v1
Checksum:iB787BA1592661FEE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF157006 mRNA Translation: AAF24175.1
UniGeneiMm.27984

Similar proteinsi

Entry informationi

Entry nameiZO3_MOUSE
AccessioniPrimary (citable) accession number: Q9QXY1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: March 28, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health