Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium-binding mitochondrial carrier protein Aralar2

Gene

Slc25a13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi66 – 771Add BLAST12
Calcium bindingi100 – 1112Add BLAST12
Calcium bindingi171 – 1823Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: MGI
  • L-aspartate transmembrane transporter activity Source: UniProtKB
  • L-glutamate transmembrane transporter activity Source: MGI

GO - Biological processi

  • aspartate transport Source: MGI
  • ATP biosynthetic process Source: MGI
  • cellular respiration Source: MGI
  • L-glutamate transport Source: UniProtKB
  • malate-aspartate shuttle Source: MGI
  • response to calcium ion Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Transport

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-70263. Gluconeogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-binding mitochondrial carrier protein Aralar2
Alternative name(s):
Citrin
Mitochondrial aspartate glutamate carrier 2
Solute carrier family 25 member 13
Gene namesi
Name:Slc25a13
Synonyms:Aralar2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1354721. Slc25a13.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei333 – 350Helical; Name=1Sequence analysisAdd BLAST18
Transmembranei394 – 413Helical; Name=2Sequence analysisAdd BLAST20
Transmembranei437 – 450Helical; Name=3Sequence analysisAdd BLAST14
Transmembranei486 – 505Helical; Name=4Sequence analysisAdd BLAST20
Transmembranei525 – 542Helical; Name=5Sequence analysisAdd BLAST18
Transmembranei582 – 601Helical; Name=6Sequence analysisAdd BLAST20

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000906022 – 676Calcium-binding mitochondrial carrier protein Aralar2Add BLAST675

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei18N6-acetyllysineCombined sources1
Modified residuei354N6-acetyllysineCombined sources1
Modified residuei373N6-acetyllysineCombined sources1
Modified residuei454N6-methyllysineBy similarity1
Modified residuei485N6-acetyllysine; alternateCombined sources1
Modified residuei485N6-succinyllysine; alternateCombined sources1
Modified residuei581N6-succinyllysineCombined sources1
Modified residuei663N6-acetyllysineCombined sources1
Modified residuei667PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiQ9QXX4.
MaxQBiQ9QXX4.
PaxDbiQ9QXX4.
PRIDEiQ9QXX4.

PTM databases

iPTMnetiQ9QXX4.
PhosphoSitePlusiQ9QXX4.
SwissPalmiQ9QXX4.

Expressioni

Tissue specificityi

At E10.45, expressed in branchial arches and lrfimb and tail buds. At E13.5 expression is predominant in epithelial structures and the forebrain, kidney and liver. Expression in liver is maintained into adulthood.1 Publication

Gene expression databases

BgeeiENSMUSG00000015112.
GenevisibleiQ9QXX4. MM.

Interactioni

Protein-protein interaction databases

BioGridi206130. 5 interactors.
IntActiQ9QXX4. 5 interactors.
MINTiMINT-1841013.
STRINGi10090.ENSMUSP00000015256.

Structurei

3D structure databases

ProteinModelPortaliQ9QXX4.
SMRiQ9QXX4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 86EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini87 – 122EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini123 – 157EF-hand 3PROSITE-ProRule annotationAdd BLAST35
Domaini158 – 193EF-hand 4PROSITE-ProRule annotationAdd BLAST36
Repeati327 – 419Solcar 1Add BLAST93
Repeati427 – 511Solcar 2Add BLAST85
Repeati519 – 607Solcar 3Add BLAST89

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation
Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0751. Eukaryota.
ENOG410XNRM. LUCA.
GeneTreeiENSGT00530000062944.
HOGENOMiHOG000180633.
HOVERGENiHBG005350.
InParanoidiQ9QXX4.
KOiK15105.
OMAiKXMPAAS.
OrthoDBiEOG091G08GT.
PhylomeDBiQ9QXX4.
TreeFamiTF313209.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
1.50.40.10. 1 hit.
InterProiIPR029658. Aralar2.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PANTHERiPTHR24089:SF265. PTHR24089:SF265. 1 hit.
PfamiPF13833. EF-hand_8. 1 hit.
PF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
SSF47473. SSF47473. 2 hits.
PROSITEiPS50222. EF_HAND_2. 3 hits.
PS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QXX4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAKVALTK RADPAELKAI FLKYASIEKN GEFFMSPHDF VTRYLNIFGE
60 70 80 90 100
SQPNPKTVEL LSGVVDQTKD GLISFQEFVA FESVLCAPDA LFMVAFQLFD
110 120 130 140 150
KAGKGEVTFE DVKQIFGQTT IHQHIPFNWD SEFVQLHFGK ERKRHLTYAE
160 170 180 190 200
FTQFLLEIQL EHAKQAFVQR DNAKTGKVSA IDFRDIMVTI RPHVLTPFVE
210 220 230 240 250
ECLVAAAGGT RSHQVSFSYF NGFNSLLNNM ELIRKIYSTL AGNRKDVEVT
260 270 280 290 300
KEEFALAAQK FGQVTPMEVD ILFQLADLYE PRGRMTLADI ERIAPLEEGM
310 320 330 340 350
LPFNLAEAQR QQKASGDAAR PFLLQLAESA YRFGLGSIAG AVGATAVYPI
360 370 380 390 400
DLVKTRMQNQ RSTGSFVGEL MYKNSFDCFK KVLRYEGFFG LYRGLLPQLL
410 420 430 440 450
GVAPEKAIKL TVNDFVRDKF MHKDGSVPLL AEIFAGGCAG GSQVIFTNPL
460 470 480 490 500
EIVKIRLQVA GEITTGPRVS ALSVVRDLGF FGIYKGAKAC FLRDIPFSAI
510 520 530 540 550
YFPCYAHVKA SFANEDGQVS PGSLLLAGAI AGMPAASLVT PADVIKTRLQ
560 570 580 590 600
VAARAGQTTY NGVTDCFRKI LREEGPKALW KGVAARVFRS SPQFGVTLLT
610 620 630 640 650
YELLQRWFYV DFGGVKPVGS EPVPKSRITL PAPNPDHVGG YKLAVATFAG
660 670
IENKFGLYLP LFKPSASTSK VTAGDS
Length:676
Mass (Da):74,467
Last modified:May 1, 2000 - v1
Checksum:i5E3952F08E5E58C5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti312Missing in BAB28397 (PubMed:16141072).Curated1
Sequence conflicti442S → F in BAB22390 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF164632 mRNA. Translation: AAF21426.1.
AK002829 mRNA. Translation: BAB22390.1.
AK012670 mRNA. Translation: BAB28397.1.
CCDSiCCDS39421.1.
RefSeqiNP_001171043.1. NM_001177572.1.
NP_056644.1. NM_015829.3.
UniGeneiMm.24513.

Genome annotation databases

EnsembliENSMUST00000015256; ENSMUSP00000015256; ENSMUSG00000015112.
ENSMUST00000188414; ENSMUSP00000139571; ENSMUSG00000015112.
GeneIDi50799.
KEGGimmu:50799.
UCSCiuc009awp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF164632 mRNA. Translation: AAF21426.1.
AK002829 mRNA. Translation: BAB22390.1.
AK012670 mRNA. Translation: BAB28397.1.
CCDSiCCDS39421.1.
RefSeqiNP_001171043.1. NM_001177572.1.
NP_056644.1. NM_015829.3.
UniGeneiMm.24513.

3D structure databases

ProteinModelPortaliQ9QXX4.
SMRiQ9QXX4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206130. 5 interactors.
IntActiQ9QXX4. 5 interactors.
MINTiMINT-1841013.
STRINGi10090.ENSMUSP00000015256.

PTM databases

iPTMnetiQ9QXX4.
PhosphoSitePlusiQ9QXX4.
SwissPalmiQ9QXX4.

Proteomic databases

EPDiQ9QXX4.
MaxQBiQ9QXX4.
PaxDbiQ9QXX4.
PRIDEiQ9QXX4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000015256; ENSMUSP00000015256; ENSMUSG00000015112.
ENSMUST00000188414; ENSMUSP00000139571; ENSMUSG00000015112.
GeneIDi50799.
KEGGimmu:50799.
UCSCiuc009awp.2. mouse.

Organism-specific databases

CTDi10165.
MGIiMGI:1354721. Slc25a13.

Phylogenomic databases

eggNOGiKOG0751. Eukaryota.
ENOG410XNRM. LUCA.
GeneTreeiENSGT00530000062944.
HOGENOMiHOG000180633.
HOVERGENiHBG005350.
InParanoidiQ9QXX4.
KOiK15105.
OMAiKXMPAAS.
OrthoDBiEOG091G08GT.
PhylomeDBiQ9QXX4.
TreeFamiTF313209.

Enzyme and pathway databases

ReactomeiR-MMU-70263. Gluconeogenesis.

Miscellaneous databases

ChiTaRSiSlc25a13. mouse.
PROiQ9QXX4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000015112.
GenevisibleiQ9QXX4. MM.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
1.50.40.10. 1 hit.
InterProiIPR029658. Aralar2.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PANTHERiPTHR24089:SF265. PTHR24089:SF265. 1 hit.
PfamiPF13833. EF-hand_8. 1 hit.
PF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
SSF47473. SSF47473. 2 hits.
PROSITEiPS50222. EF_HAND_2. 3 hits.
PS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCMC2_MOUSE
AccessioniPrimary (citable) accession number: Q9QXX4
Secondary accession number(s): Q9CZF6, Q9DCF5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.