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Protein

Group 10 secretory phospholipase A2

Gene

Pla2g10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a powerful potency for releasing arachidonic acid from cell membrane phospholipids.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Calcium; via carbonyl oxygenBy similarity
Metal bindingi56 – 561Calcium; via carbonyl oxygenBy similarity
Metal bindingi58 – 581Calcium; via carbonyl oxygenBy similarity
Active sitei74 – 741By similarity
Metal bindingi75 – 751CalciumBy similarity
Active sitei119 – 1191By similarity

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. phospholipase A2 activity Source: UniProtKB-EC
  3. phospholipase activity Source: BHF-UCL

GO - Biological processi

  1. axon guidance Source: MGI
  2. cholesterol homeostasis Source: BHF-UCL
  3. lipid catabolic process Source: UniProtKB-KW
  4. lysophospholipid transport Source: MGI
  5. negative regulation of cholesterol efflux Source: BHF-UCL
  6. negative regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  7. phospholipid metabolic process Source: InterPro
  8. positive regulation of arachidonic acid secretion Source: BHF-UCL
  9. positive regulation of cellular protein metabolic process Source: MGI
  10. positive regulation of lipid storage Source: MGI
  11. positive regulation of prostaglandin secretion Source: MGI
  12. regulation of macrophage activation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_188640. Synthesis of PA.
REACT_199024. Acyl chain remodelling of PI.
REACT_199027. Acyl chain remodelling of PE.
REACT_199030. Acyl chain remodelling of PS.
REACT_199038. Acyl chain remodelling of PC.
REACT_199039. Acyl chain remodelling of PG.

Names & Taxonomyi

Protein namesi
Recommended name:
Group 10 secretory phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Group X secretory phospholipase A2
Short name:
GX sPLA2
Short name:
sPLA2-X
Phosphatidylcholine 2-acylhydrolase 10
Gene namesi
Name:Pla2g10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1347522. Pla2g10.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17171 PublicationAdd
BLAST
Propeptidei18 – 2811PRO_0000022766Add
BLAST
Chaini29 – 151123Group 10 secretory phospholipase A2PRO_0000022767Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi39 ↔ 97By similarity
Disulfide bondi53 ↔ 143By similarity
Disulfide bondi55 ↔ 71By similarity
Disulfide bondi70 ↔ 125By similarity
Disulfide bondi76 ↔ 150By similarity
Disulfide bondi77 ↔ 118By similarity
Disulfide bondi86 ↔ 111By similarity
Disulfide bondi104 ↔ 116By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PRIDEiQ9QXX3.

Expressioni

Tissue specificityi

Expressed in various tissues including the lung, thymus, and spleen.

Gene expression databases

BgeeiQ9QXX3.
CleanExiMM_PLA2G10.
ExpressionAtlasiQ9QXX3. baseline and differential.
GenevestigatoriQ9QXX3.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023364.

Structurei

3D structure databases

ProteinModelPortaliQ9QXX3.
SMRiQ9QXX3. Positions 29-151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG284162.
GeneTreeiENSGT00760000119160.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiQ9QXX3.
KOiK01047.
OMAiCQELLCK.
PhylomeDBiQ9QXX3.
TreeFamiTF319283.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QXX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLLLLLLLL GPGPGFSEAT RRSHVYKRGL LELAGTLDCV GPRSPMAYMN
60 70 80 90 100
YGCYCGLGGH GEPRDAIDWC CYHHDCCYSR AQDAGCSPKL DRYPWKCMDH
110 120 130 140 150
HILCGPAENK CQELLCRCDE ELAYCLAGTE YHLKYLFFPS ILCEKDSPKC

N
Length:151
Mass (Da):17,005
Last modified:May 1, 2000 - v1
Checksum:i05D15E70BC2C9294
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti151 – 1511N → D in AAG43522 (PubMed:11019817).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF166097 mRNA. Translation: AAF04498.2.
AF210429 mRNA. Translation: AAG43522.1.
CCDSiCCDS27967.1.
RefSeqiNP_036117.1. NM_011987.3.
UniGeneiMm.4214.

Genome annotation databases

EnsembliENSMUST00000023364; ENSMUSP00000023364; ENSMUSG00000022683.
GeneIDi26565.
KEGGimmu:26565.
UCSCiuc007ygh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF166097 mRNA. Translation: AAF04498.2.
AF210429 mRNA. Translation: AAG43522.1.
CCDSiCCDS27967.1.
RefSeqiNP_036117.1. NM_011987.3.
UniGeneiMm.4214.

3D structure databases

ProteinModelPortaliQ9QXX3.
SMRiQ9QXX3. Positions 29-151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023364.

Chemistry

BindingDBiQ9QXX3.
ChEMBLiCHEMBL4200.

Proteomic databases

PRIDEiQ9QXX3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023364; ENSMUSP00000023364; ENSMUSG00000022683.
GeneIDi26565.
KEGGimmu:26565.
UCSCiuc007ygh.1. mouse.

Organism-specific databases

CTDi8399.
MGIiMGI:1347522. Pla2g10.

Phylogenomic databases

eggNOGiNOG284162.
GeneTreeiENSGT00760000119160.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiQ9QXX3.
KOiK01047.
OMAiCQELLCK.
PhylomeDBiQ9QXX3.
TreeFamiTF319283.

Enzyme and pathway databases

ReactomeiREACT_188640. Synthesis of PA.
REACT_199024. Acyl chain remodelling of PI.
REACT_199027. Acyl chain remodelling of PE.
REACT_199030. Acyl chain remodelling of PS.
REACT_199038. Acyl chain remodelling of PC.
REACT_199039. Acyl chain remodelling of PG.

Miscellaneous databases

NextBioi304643.
PROiQ9QXX3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QXX3.
CleanExiMM_PLA2G10.
ExpressionAtlasiQ9QXX3. baseline and differential.
GenevestigatoriQ9QXX3.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "On the diversity of secreted phospholipases A2. Cloning, tissue distribution, and functional expression of two novel mouse group II enzymes."
    Valentin E., Ghomashchi F., Gelb M.H., Lazdunski M., Lambeau G.
    J. Biol. Chem. 274:31195-31202(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Mouse group X secretory phospholipase A2 induces a potent release of arachidonic acid from spleen cells and acts as a ligand for the phospholipase A2 receptor."
    Morioka Y., Saiga A., Yokota Y., Suzuki N., Ikeda M., Ono T., Nakano K., Fujii N., Ishizaki J., Arita H., Hanasaki K.
    Arch. Biochem. Biophys. 381:31-42(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-30, CHARACTERIZATION.
    Strain: BALB/c.

Entry informationi

Entry nameiPA2GX_MOUSE
AccessioniPrimary (citable) accession number: Q9QXX3
Secondary accession number(s): Q9EQK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: May 1, 2000
Last modified: February 4, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.