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Q9QXX0 (JAG1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein jagged-1

Short name=Jagged1
Alternative name(s):
CD_antigen=CD339
Gene names
Name:Jag1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1218 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Ligand for multiple Notch receptors and involved in the mediation of Notch signaling. May be involved in cell-fate decisions during hematopoiesis. Seems to be involved in early and late stages of mammalian cardiovascular development. Inhibits myoblast differentiation By similarity. May regulate fibroblast growth factor-induced angiogenesis.

Subunit structure

Interacts with NOTCH1, NOTCH2 and NOTCH3. Ref.1

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Widely expressed in many tissues, with highest expression in brain, heart, muscle and thymus. Ref.3

Developmental stage

At 8.75-9.75 dpc expression was detected in structures that include those destined to contribute to the cardiovascular system of the adult heart. Expression was also detected in the mesencephalon and rhombencephalon.

Domain

The DSL domain is indispensable and sufficient for binding to NOTCH2.

Sequence similarities

Contains 1 DSL domain.

Contains 16 EGF-like domains.

Ontologies

Keywords
   Biological processNotch signaling pathway
   Cellular componentMembrane
   DomainEGF-like domain
Repeat
Signal
Transmembrane
Transmembrane helix
   LigandCalcium
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processNotch signaling involved in heart development

Inferred from mutant phenotype PubMed 19509466. Source: BHF-UCL

Notch signaling pathway

Traceable author statement PubMed 15821257. Source: UniProtKB

aorta morphogenesis

Inferred from mutant phenotype PubMed 19509466. Source: BHF-UCL

auditory receptor cell differentiation

Inferred from mutant phenotype PubMed 16495313. Source: MGI

cardiac neural crest cell development involved in outflow tract morphogenesis

Inferred from mutant phenotype PubMed 19509466. Source: BHF-UCL

cardiac right ventricle morphogenesis

Inferred from mutant phenotype PubMed 19509466. Source: BHF-UCL

cardiac septum morphogenesis

Inferred from mutant phenotype PubMed 19509466. Source: BHF-UCL

ciliary body morphogenesis

Inferred from mutant phenotype PubMed 23676271. Source: MGI

distal tubule development

Inferred from expression pattern PubMed 15821257. Source: UniProtKB

endocardial cushion cell development

Inferred from mutant phenotype PubMed 19509466. Source: BHF-UCL

inner ear development

Inferred from mutant phenotype PubMed 19389353. Source: UniProtKB

loop of Henle development

Inferred from expression pattern PubMed 15821257. Source: UniProtKB

morphogenesis of an epithelial sheet

Inferred from mutant phenotype PubMed 11861489. Source: MGI

negative regulation of cell differentiation

Inferred from mutant phenotype PubMed 15064243. Source: MGI

negative regulation of fat cell differentiation

Inferred from direct assay PubMed 15060169. Source: MGI

negative regulation of neuron differentiation

Inferred from mutant phenotype PubMed 20081190. Source: MGI

negative regulation of stem cell differentiation

Inferred from electronic annotation. Source: Ensembl

neuronal stem cell maintenance

Inferred from electronic annotation. Source: Ensembl

organ morphogenesis

Inferred from mutant phenotype PubMed 11181574. Source: MGI

positive regulation of Notch signaling pathway

Inferred from direct assay PubMed 15574878PubMed 15845452. Source: MGI

positive regulation of myeloid cell differentiation

Inferred from direct assay PubMed 15845452. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 19509466. Source: BHF-UCL

pulmonary artery morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

pulmonary valve morphogenesis

Inferred from sequence or structural similarity. Source: BHF-UCL

response to muramyl dipeptide

Inferred from direct assay PubMed 21156799. Source: BHF-UCL

   Cellular_componentapical part of cell

Inferred from direct assay PubMed 15821257. Source: UniProtKB

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from direct assay PubMed 16000382PubMed 16495313. Source: MGI

   Molecular_functionNotch binding

Inferred from physical interaction Ref.1. Source: UniProtKB

calcium ion binding

Non-traceable author statement Ref.1. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3333 Potential
Chain34 – 12181185Protein jagged-1
PRO_0000007626

Regions

Topological domain34 – 10671034Extracellular Potential
Transmembrane1068 – 109326Helical; Potential
Topological domain1094 – 1218125Cytoplasmic Potential
Domain185 – 22945DSL
Domain230 – 26334EGF-like 1
Domain264 – 29431EGF-like 2; atypical
Domain296 – 33439EGF-like 3
Domain336 – 37237EGF-like 4
Domain374 – 41037EGF-like 5; calcium-binding Potential
Domain412 – 44837EGF-like 6; calcium-binding Potential
Domain450 – 48536EGF-like 7; calcium-binding Potential
Domain487 – 52337EGF-like 8; calcium-binding Potential
Domain525 – 56137EGF-like 9
Domain586 – 62742EGF-like 10
Domain629 – 66537EGF-like 11; calcium-binding Potential
Domain667 – 70337EGF-like 12; calcium-binding Potential
Domain705 – 74137EGF-like 13
Domain744 – 78037EGF-like 14
Domain782 – 81837EGF-like 15; calcium-binding Potential
Domain820 – 85637EGF-like 16; calcium-binding Potential
Region199 – 2079Important for interaction with NOTCH1 By similarity

Amino acid modifications

Glycosylation1431N-linked (GlcNAc...) Potential
Glycosylation2171N-linked (GlcNAc...) Potential
Glycosylation3821N-linked (GlcNAc...) Potential
Glycosylation5591N-linked (GlcNAc...) Potential
Glycosylation7451N-linked (GlcNAc...) Potential
Glycosylation9601N-linked (GlcNAc...) Potential
Glycosylation9911N-linked (GlcNAc...) Potential
Glycosylation10451N-linked (GlcNAc...) Potential
Glycosylation10641N-linked (GlcNAc...) Potential
Disulfide bond187 ↔ 196 By similarity
Disulfide bond200 ↔ 212 By similarity
Disulfide bond220 ↔ 229 By similarity
Disulfide bond234 ↔ 245 By similarity
Disulfide bond238 ↔ 251 By similarity
Disulfide bond253 ↔ 262 By similarity
Disulfide bond265 ↔ 276 By similarity
Disulfide bond271 ↔ 282 By similarity
Disulfide bond284 ↔ 293 By similarity
Disulfide bond300 ↔ 312 By similarity
Disulfide bond306 ↔ 322 By similarity
Disulfide bond324 ↔ 333 By similarity
Disulfide bond340 ↔ 351 By similarity
Disulfide bond345 ↔ 360 By similarity
Disulfide bond362 ↔ 371 By similarity
Disulfide bond378 ↔ 389 By similarity
Disulfide bond383 ↔ 398 By similarity
Disulfide bond400 ↔ 409 By similarity
Disulfide bond416 ↔ 427 By similarity
Disulfide bond421 ↔ 436 By similarity
Disulfide bond438 ↔ 447 By similarity
Disulfide bond454 ↔ 464 By similarity
Disulfide bond458 ↔ 473 By similarity
Disulfide bond475 ↔ 484 By similarity
Disulfide bond491 ↔ 502 By similarity
Disulfide bond496 ↔ 511 By similarity
Disulfide bond513 ↔ 522 By similarity
Disulfide bond529 ↔ 540 By similarity
Disulfide bond534 ↔ 549 By similarity
Disulfide bond551 ↔ 560 By similarity
Disulfide bond578 ↔ 605 By similarity
Disulfide bond599 ↔ 615 By similarity
Disulfide bond617 ↔ 626 By similarity
Disulfide bond633 ↔ 644 By similarity
Disulfide bond638 ↔ 653 By similarity
Disulfide bond655 ↔ 664 By similarity
Disulfide bond671 ↔ 682 By similarity
Disulfide bond676 ↔ 691 By similarity
Disulfide bond693 ↔ 702 By similarity
Disulfide bond709 ↔ 720 By similarity
Disulfide bond714 ↔ 729 By similarity
Disulfide bond731 ↔ 740 By similarity
Disulfide bond748 ↔ 759 By similarity
Disulfide bond753 ↔ 768 By similarity
Disulfide bond770 ↔ 779 By similarity
Disulfide bond786 ↔ 797 By similarity
Disulfide bond791 ↔ 806 By similarity
Disulfide bond808 ↔ 817 By similarity
Disulfide bond824 ↔ 835 By similarity
Disulfide bond829 ↔ 844 By similarity
Disulfide bond846 ↔ 855 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9QXX0 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 77739F8928BB793C

FASTA1,218134,164
        10         20         30         40         50         60 
MRSPRTRGRP GRPLSLLLAL LCALRAKVCG ASGQFELEIL SMQNVNGELQ NGNCCGGVRN 

        70         80         90        100        110        120 
PGDRKCTRDE CDTYFKVCLK EYQSRVTAGG PCSFGSGSTP VIGGNTFNLK ASRGNDRNRI 

       130        140        150        160        170        180 
VLPFSFAWPR SYTLLVEAWD SSNDTIQPDS IIEKASHSGM INPSRQWQTL KQNTGIAHFE 

       190        200        210        220        230        240 
YQIRVTCDDH YYGFGCNKFC RPRDDFFGHY ACDQNGNKTC MEGWMGPDCN KAICRQGCSP 

       250        260        270        280        290        300 
KHGSCKLPGD CRCQYGWQGL YCDKCIPHPG CVHGTCNEPW QCLCETNWGG QLCDKDLNYC 

       310        320        330        340        350        360 
GTHQPCLNRG TCSNTGPDKY QCSCPEGYSG PNCEIAEHAC LSDPCHNRGS CKETSSGFEC 

       370        380        390        400        410        420 
ECSPGWTGPT CSTNIDDCSP NNCSHGGTCQ DLVNGFKCVC PPQWTGKTCQ LDANECEAKP 

       430        440        450        460        470        480 
CVNARSCKNL IASYYCDCLP GWMGQNCDIN INDCLGQCQN DASCRDLVNG YRCICPPGYA 

       490        500        510        520        530        540 
GDHCERDIDE CASNPCLNGG HCQNEINRFQ CLCPTGFSGN LCQLDIDYCE PNPCQNGAQC 

       550        560        570        580        590        600 
YNRASDYFCK CPEDYEGKNC SHLKDHCRTT TCEVIDSCTV AMASNDTPEG VRYISSNVCG 

       610        620        630        640        650        660 
PHGKCKSQSG GKFTCDCNKG FTGTYCHENI NDCESNPCKN GGTCIDGVNS YKCICSDGWE 

       670        680        690        700        710        720 
GAHCENNIND CSQNPCHYGG TCRDLVNDFY CDCKNGWKGK TCHSRDSQCD EATCNNGGTC 

       730        740        750        760        770        780 
YDEVDTFKCM CPGGWEGTTC NIARNSSCLP NPCHNGGTCV VNGDSFTCVC KEGWEGPICT 

       790        800        810        820        830        840 
QNTNDCSPHP CYNSGTCVDG DNWYRCECAP GFAGPDCRIN INECQSSPCA FGATCVDEIN 

       850        860        870        880        890        900 
GYQCICPPGH SGAKCHEVSG RSCITMGRVI LDGAKWDDDC NTCQCLNGRV ACSKVWCGPR 

       910        920        930        940        950        960 
PCRLHKSHNE CPSGQSCIPV LDDQCFVRPC TGVGECRSSS LQPVKTKCTS DSYYQDNCAN 

       970        980        990       1000       1010       1020 
ITFTFNKEMM SPGLTTEHIC SELRNLNILK NVSAEYSIYI ACEPSLSANN EIHVAISAED 

      1030       1040       1050       1060       1070       1080 
IRDDGNPVKE ITDKIIDLVS KRDGNSSLIA AVAEVRVQRR PLKNRTDFLV PLLSSVLTVA 

      1090       1100       1110       1120       1130       1140 
WVCCLVTAFY WCVRKRRKPS SHTHSAPEDN TTNNVREQLN QIKNPIEKHG ANTVPIKDYE 

      1150       1160       1170       1180       1190       1200 
NKNSKMSKIR THNSEVEEDD MDKHQQKVRF AKQPVYTLVD REEKAPSGTP TKHPNWTNKQ 

      1210 
DNRDLESAQS LNRMEYIV 

« Hide

References

« Hide 'large scale' references
[1]"Mouse Jagged1 physically interacts with Notch2 and other Notch receptors: assessment by quantitative methods."
Shimizu K., Chiba S., Kumano K., Hosoya N., Takahashi T., Kanda Y., Hamada Y., Yazaki Y., Hirai H.
J. Biol. Chem. 274:32961-32969(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], RECEPTOR INTERACTION.
Strain: Swiss Webster / NIH.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"The expression of Jagged1 in the developing mammalian heart correlates with cardiovascular disease in Alagille syndrome."
Loomes K.M., Underkoffler L.A., Morabito J., Gottlieb S., Piccoli D.A., Spinner N.B., Baldwin H.S., Oakey R.J.
Hum. Mol. Genet. 8:2443-2449(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF171092 mRNA. Translation: AAF15505.1.
BC058675 mRNA. Translation: AAH58675.1.
RefSeqNP_038850.1. NM_013822.5.
UniGeneMm.22398.

3D structure databases

ProteinModelPortalQ9QXX0.
SMRQ9QXX0. Positions 186-930.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200854. 1 interaction.

PTM databases

PhosphoSiteQ9QXX0.

Proteomic databases

PaxDbQ9QXX0.
PRIDEQ9QXX0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028735; ENSMUSP00000028735; ENSMUSG00000027276.
GeneID16449.
KEGGmmu:16449.
UCSCuc008moz.2. mouse.

Organism-specific databases

CTD182.
MGIMGI:1095416. Jag1.

Phylogenomic databases

eggNOGNOG12793.
GeneTreeENSGT00740000114998.
HOGENOMHOG000113124.
HOVERGENHBG031645.
InParanoidQ9QXX0.
KOK06052.
OMAHPNWTNK.
OrthoDBEOG7BKCSS.
PhylomeDBQ9QXX0.
TreeFamTF351835.

Enzyme and pathway databases

ReactomeREACT_106849. Receptor-ligand binding initiates the second proteolytic cleavage of Notch receptor.
REACT_188257. Signal Transduction.
REACT_189085. Disease.

Gene expression databases

ArrayExpressQ9QXX0.
BgeeQ9QXX0.
CleanExMM_JAG1.
GenevestigatorQ9QXX0.

Family and domain databases

InterProIPR001774. DSL.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR009030. Growth_fac_rcpt_N_dom.
IPR026219. Jagged/Serrate.
IPR011651. Notch_ligand_N.
IPR001007. VWF_C.
[Graphical view]
PfamPF01414. DSL. 1 hit.
PF00008. EGF. 11 hits.
PF07645. EGF_CA. 1 hit.
PF07657. MNNL. 1 hit.
[Graphical view]
PRINTSPR02059. JAGGEDFAMILY.
SMARTSM00051. DSL. 1 hit.
SM00181. EGF. 6 hits.
SM00179. EGF_CA. 10 hits.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMSSF57184. SSF57184. 3 hits.
PROSITEPS00010. ASX_HYDROXYL. 10 hits.
PS51051. DSL. 1 hit.
PS00022. EGF_1. 16 hits.
PS01186. EGF_2. 12 hits.
PS50026. EGF_3. 15 hits.
PS01187. EGF_CA. 8 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSJAG1. mouse.
NextBio289705.
PROQ9QXX0.
SOURCESearch...

Entry information

Entry nameJAG1_MOUSE
AccessionPrimary (citable) accession number: Q9QXX0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot