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Q9QXW9

- LAT2_MOUSE

UniProt

Q9QXW9 - LAT2_MOUSE

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Protein

Large neutral amino acids transporter small subunit 2

Gene

Slc7a8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.1 Publication

Kineticsi

  1. KM=12.2 µM for L-phenylalanine1 Publication
  2. KM=48 µM for L-leucine1 Publication
  3. KM=167 µM for L-alanine1 Publication
  4. KM=275 µM for L-glutamine1 Publication
  5. KM=294 µM for L-histidine1 Publication

GO - Molecular functioni

  1. amine transmembrane transporter activity Source: MGI
  2. L-amino acid transmembrane transporter activity Source: MGI
  3. organic cation transmembrane transporter activity Source: Ensembl
  4. toxin transporter activity Source: Ensembl

GO - Biological processi

  1. amine transport Source: GOC
  2. amino acid transport Source: MGI
  3. L-alpha-amino acid transmembrane transport Source: GOC
  4. L-amino acid transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

ReactomeiREACT_211508. Basigin interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Large neutral amino acids transporter small subunit 2
Alternative name(s):
L-type amino acid transporter 2
Short name:
mLAT2
Solute carrier family 7 member 8
Gene namesi
Name:Slc7a8
Synonyms:Lat2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:1355323. Slc7a8.

Subcellular locationi

Cytoplasm By similarity. Basolateral cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Localized to the cytoplasm when expressed alone By similarity. When coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. extracellular vesicular exosome Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
  4. plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 531531Large neutral amino acids transporter small subunit 2PRO_0000054274Add
BLAST

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiQ9QXW9.
PaxDbiQ9QXW9.
PRIDEiQ9QXW9.

PTM databases

PhosphoSiteiQ9QXW9.

Expressioni

Tissue specificityi

Strongly expressed in kidney and small intestine. Moderately present in placenta, ovary and brain.1 Publication

Gene expression databases

BgeeiQ9QXW9.
CleanExiMM_LAT2.
GenevestigatoriQ9QXW9.

Interactioni

Subunit structurei

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.1 Publication

Protein-protein interaction databases

BioGridi206166. 1 interaction.
STRINGi10090.ENSMUSP00000022787.

Structurei

3D structure databases

ProteinModelPortaliQ9QXW9.
SMRiQ9QXW9. Positions 38-368.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei39 – 5921HelicalSequence AnalysisAdd
BLAST
Transmembranei71 – 9121HelicalSequence AnalysisAdd
BLAST
Transmembranei112 – 13221HelicalSequence AnalysisAdd
BLAST
Transmembranei154 – 17421HelicalSequence AnalysisAdd
BLAST
Transmembranei188 – 20821HelicalSequence AnalysisAdd
BLAST
Transmembranei230 – 25021HelicalSequence AnalysisAdd
BLAST
Transmembranei267 – 28721HelicalSequence AnalysisAdd
BLAST
Transmembranei309 – 32921HelicalSequence AnalysisAdd
BLAST
Transmembranei361 – 38121HelicalSequence AnalysisAdd
BLAST
Transmembranei387 – 40721HelicalSequence AnalysisAdd
BLAST
Transmembranei421 – 44121HelicalSequence AnalysisAdd
BLAST
Transmembranei446 – 46621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0531.
GeneTreeiENSGT00760000119037.
HOGENOMiHOG000098892.
HOVERGENiHBG000476.
InParanoidiQ9QXW9.
KOiK13781.
OMAiAFEPFQD.
OrthoDBiEOG73BVCR.
PhylomeDBiQ9QXW9.
TreeFamiTF313355.

Family and domain databases

InterProiIPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view]
PANTHERiPTHR11785. PTHR11785. 1 hit.
PfamiPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsiTIGR00911. 2A0308. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9QXW9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKGARQRNN TAKNHPGSDT SPEAEASSGG GGVALKKEIG LVSACGIIVG
60 70 80 90 100
NIIGSGIFVS PKGVLENAGS VGLALIVWIV TGIITAVGAL CYAELGVTIP
110 120 130 140 150
KSGGDYSYVK DIFGGLAGFL RLWIAVLVIY PTNQAVIALT FSNYVLQPLF
160 170 180 190 200
PTCFPPESGL RLLAAICLLL LTWVNCSSVR WATRVQDIFT AGKLLALALI
210 220 230 240 250
IIMGIVQICK GEFFWLEPKN AFENFQEPDI GLVALAFLQG SFAYGGWNFL
260 270 280 290 300
NYVTEELVDP YKNLPRAIFI SIPLVTFVYV FANIAYVTAM SPQELLASNA
310 320 330 340 350
VAVTFGEKLL GVMAWIMPIS VALSTFGGVN GSLFTSSRLF FAGAREGHLP
360 370 380 390 400
SVLAMIHVKR CTPIPALLFT CLSTLLMLVT SDMYTLINYV GFINYLFYGV
410 420 430 440 450
TVAGQIVLRW KKPDIPRPIK VSLLFPIIYL LFWAFLLIFS LWSEPVVCGI
460 470 480 490 500
GLAIMLTGVP VYFLGVYWQH KPKCFNDFIK SLTLVSQKMC VVVYPQEGNS
510 520 530
GAEETTDDLE EQHKPIFKPT PVKDPDSEEQ P
Length:531
Mass (Da):57,873
Last modified:May 1, 2000 - v1
Checksum:iAE9C3B42F3B24F8C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF171668 mRNA. Translation: AAF20380.1.
Y19022 mRNA. Translation: CAB69072.1.
BC059004 mRNA. Translation: AAH59004.1.
CCDSiCCDS27101.1.
RefSeqiNP_058668.1. NM_016972.2.
UniGeneiMm.276831.

Genome annotation databases

EnsembliENSMUST00000022787; ENSMUSP00000022787; ENSMUSG00000022180.
GeneIDi50934.
KEGGimmu:50934.
UCSCiuc007txa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF171668 mRNA. Translation: AAF20380.1 .
Y19022 mRNA. Translation: CAB69072.1 .
BC059004 mRNA. Translation: AAH59004.1 .
CCDSi CCDS27101.1.
RefSeqi NP_058668.1. NM_016972.2.
UniGenei Mm.276831.

3D structure databases

ProteinModelPortali Q9QXW9.
SMRi Q9QXW9. Positions 38-368.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 206166. 1 interaction.
STRINGi 10090.ENSMUSP00000022787.

PTM databases

PhosphoSitei Q9QXW9.

Proteomic databases

MaxQBi Q9QXW9.
PaxDbi Q9QXW9.
PRIDEi Q9QXW9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022787 ; ENSMUSP00000022787 ; ENSMUSG00000022180 .
GeneIDi 50934.
KEGGi mmu:50934.
UCSCi uc007txa.1. mouse.

Organism-specific databases

CTDi 23428.
MGIi MGI:1355323. Slc7a8.

Phylogenomic databases

eggNOGi COG0531.
GeneTreei ENSGT00760000119037.
HOGENOMi HOG000098892.
HOVERGENi HBG000476.
InParanoidi Q9QXW9.
KOi K13781.
OMAi AFEPFQD.
OrthoDBi EOG73BVCR.
PhylomeDBi Q9QXW9.
TreeFami TF313355.

Enzyme and pathway databases

Reactomei REACT_211508. Basigin interactions.

Miscellaneous databases

NextBioi 307981.
PROi Q9QXW9.
SOURCEi Search...

Gene expression databases

Bgeei Q9QXW9.
CleanExi MM_LAT2.
Genevestigatori Q9QXW9.

Family and domain databases

InterProi IPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view ]
PANTHERi PTHR11785. PTHR11785. 1 hit.
Pfami PF13520. AA_permease_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006060. AA_transporter. 1 hit.
TIGRFAMsi TIGR00911. 2A0308. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
    Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C.
    J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Embryo.
  2. "SLC7A8, a gene mapping within the lysinuric protein intolerance critical region, encodes a new member of the glycoprotein-associated amino acid transporter family."
    Bassi M.T., Sperandeo M.P., Incerti B., Bulfone A., Pepe A., Surace E.M., Gattuso C., de Grandi A., Buoninconti A., Riboni M., Manzoni M., Andria G., Ballabio A., Borsani G., Sebastio G.
    Genomics 62:297-303(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.

Entry informationi

Entry nameiLAT2_MOUSE
AccessioniPrimary (citable) accession number: Q9QXW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3