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Q9QXW9 (LAT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Large neutral amino acids transporter small subunit 2
Alternative name(s):
L-type amino acid transporter 2
Short name=mLAT2
Solute carrier family 7 member 8
Gene names
Name:Slc7a8
Synonyms:Lat2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length531 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney. Ref.1

Subunit structure

Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Ref.1

Subcellular location

Cytoplasm By similarity. Basolateral cell membrane; Multi-pass membrane protein. Note: Localized to the cytoplasm when expressed alone By similarity. When coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi. Ref.1

Tissue specificity

Strongly expressed in kidney and small intestine. Moderately present in placenta, ovary and brain. Ref.1

Sequence similarities

Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=12.2 µM for L-phenylalanine Ref.1

KM=48 µM for L-leucine

KM=167 µM for L-alanine

KM=275 µM for L-glutamine

KM=294 µM for L-histidine

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 531531Large neutral amino acids transporter small subunit 2
PRO_0000054274

Regions

Transmembrane39 – 5921Helical; Potential
Transmembrane71 – 9121Helical; Potential
Transmembrane112 – 13221Helical; Potential
Transmembrane154 – 17421Helical; Potential
Transmembrane188 – 20821Helical; Potential
Transmembrane230 – 25021Helical; Potential
Transmembrane267 – 28721Helical; Potential
Transmembrane309 – 32921Helical; Potential
Transmembrane361 – 38121Helical; Potential
Transmembrane387 – 40721Helical; Potential
Transmembrane421 – 44121Helical; Potential
Transmembrane446 – 46621Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q9QXW9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: AE9C3B42F3B24F8C

FASTA53157,873
        10         20         30         40         50         60 
MEKGARQRNN TAKNHPGSDT SPEAEASSGG GGVALKKEIG LVSACGIIVG NIIGSGIFVS 

        70         80         90        100        110        120 
PKGVLENAGS VGLALIVWIV TGIITAVGAL CYAELGVTIP KSGGDYSYVK DIFGGLAGFL 

       130        140        150        160        170        180 
RLWIAVLVIY PTNQAVIALT FSNYVLQPLF PTCFPPESGL RLLAAICLLL LTWVNCSSVR 

       190        200        210        220        230        240 
WATRVQDIFT AGKLLALALI IIMGIVQICK GEFFWLEPKN AFENFQEPDI GLVALAFLQG 

       250        260        270        280        290        300 
SFAYGGWNFL NYVTEELVDP YKNLPRAIFI SIPLVTFVYV FANIAYVTAM SPQELLASNA 

       310        320        330        340        350        360 
VAVTFGEKLL GVMAWIMPIS VALSTFGGVN GSLFTSSRLF FAGAREGHLP SVLAMIHVKR 

       370        380        390        400        410        420 
CTPIPALLFT CLSTLLMLVT SDMYTLINYV GFINYLFYGV TVAGQIVLRW KKPDIPRPIK 

       430        440        450        460        470        480 
VSLLFPIIYL LFWAFLLIFS LWSEPVVCGI GLAIMLTGVP VYFLGVYWQH KPKCFNDFIK 

       490        500        510        520        530 
SLTLVSQKMC VVVYPQEGNS GAEETTDDLE EQHKPIFKPT PVKDPDSEEQ P 

« Hide

References

« Hide 'large scale' references
[1]"LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C.
J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: C57BL/6J.
Tissue: Embryo.
[2]"SLC7A8, a gene mapping within the lysinuric protein intolerance critical region, encodes a new member of the glycoprotein-associated amino acid transporter family."
Bassi M.T., Sperandeo M.P., Incerti B., Bulfone A., Pepe A., Surace E.M., Gattuso C., de Grandi A., Buoninconti A., Riboni M., Manzoni M., Andria G., Ballabio A., Borsani G., Sebastio G.
Genomics 62:297-303(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF171668 mRNA. Translation: AAF20380.1.
Y19022 mRNA. Translation: CAB69072.1.
BC059004 mRNA. Translation: AAH59004.1.
RefSeqNP_058668.1. NM_016972.2.
UniGeneMm.276831.

3D structure databases

ProteinModelPortalQ9QXW9.
SMRQ9QXW9. Positions 38-368.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid206166. 1 interaction.
STRING10090.ENSMUSP00000022787.

PTM databases

PhosphoSiteQ9QXW9.

Proteomic databases

PaxDbQ9QXW9.
PRIDEQ9QXW9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022787; ENSMUSP00000022787; ENSMUSG00000022180.
GeneID50934.
KEGGmmu:50934.
UCSCuc007txa.1. mouse.

Organism-specific databases

CTD23428.
MGIMGI:1355323. Slc7a8.

Phylogenomic databases

eggNOGCOG0531.
GeneTreeENSGT00730000110280.
HOGENOMHOG000098892.
HOVERGENHBG000476.
InParanoidQ9QXW9.
KOK13781.
OMALIVWIIT.
OrthoDBEOG73BVCR.
PhylomeDBQ9QXW9.
TreeFamTF313355.

Gene expression databases

BgeeQ9QXW9.
CleanExMM_LAT2.
GenevestigatorQ9QXW9.

Family and domain databases

InterProIPR002293. AA/rel_permease1.
IPR004760. L_AA_transporter.
[Graphical view]
PANTHERPTHR11785. PTHR11785. 1 hit.
PfamPF13520. AA_permease_2. 1 hit.
[Graphical view]
PIRSFPIRSF006060. AA_transporter. 1 hit.
TIGRFAMsTIGR00911. 2A0308. 1 hit.
ProtoNetSearch...

Other

NextBio307981.
PROQ9QXW9.
SOURCESearch...

Entry information

Entry nameLAT2_MOUSE
AccessionPrimary (citable) accession number: Q9QXW9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 2000
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot