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Q9QXW9

- LAT2_MOUSE

UniProt

Q9QXW9 - LAT2_MOUSE

Protein

Large neutral amino acids transporter small subunit 2

Gene

Slc7a8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.1 Publication

    Kineticsi

    1. KM=12.2 µM for L-phenylalanine1 Publication
    2. KM=48 µM for L-leucine1 Publication
    3. KM=167 µM for L-alanine1 Publication
    4. KM=275 µM for L-glutamine1 Publication
    5. KM=294 µM for L-histidine1 Publication

    GO - Molecular functioni

    1. amine transmembrane transporter activity Source: MGI
    2. L-amino acid transmembrane transporter activity Source: MGI
    3. organic cation transmembrane transporter activity Source: Ensembl
    4. toxin transporter activity Source: Ensembl

    GO - Biological processi

    1. amine transport Source: GOC
    2. amino acid transport Source: MGI
    3. L-alpha-amino acid transmembrane transport Source: GOC
    4. L-amino acid transport Source: MGI

    Keywords - Biological processi

    Amino-acid transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_211508. Basigin interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 2
    Alternative name(s):
    L-type amino acid transporter 2
    Short name:
    mLAT2
    Solute carrier family 7 member 8
    Gene namesi
    Name:Slc7a8
    Synonyms:Lat2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1355323. Slc7a8.

    Subcellular locationi

    Cytoplasm By similarity. Basolateral cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Localized to the cytoplasm when expressed alone By similarity. When coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi.By similarity

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB-KW
    4. plasma membrane Source: MGI

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 531531Large neutral amino acids transporter small subunit 2PRO_0000054274Add
    BLAST

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiQ9QXW9.
    PaxDbiQ9QXW9.
    PRIDEiQ9QXW9.

    PTM databases

    PhosphoSiteiQ9QXW9.

    Expressioni

    Tissue specificityi

    Strongly expressed in kidney and small intestine. Moderately present in placenta, ovary and brain.1 Publication

    Gene expression databases

    BgeeiQ9QXW9.
    CleanExiMM_LAT2.
    GenevestigatoriQ9QXW9.

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.1 Publication

    Protein-protein interaction databases

    BioGridi206166. 1 interaction.
    STRINGi10090.ENSMUSP00000022787.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QXW9.
    SMRiQ9QXW9. Positions 38-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei39 – 5921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei71 – 9121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei112 – 13221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei154 – 17421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei188 – 20821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei230 – 25021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei267 – 28721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei309 – 32921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei361 – 38121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei387 – 40721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei421 – 44121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei446 – 46621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0531.
    GeneTreeiENSGT00730000110280.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ9QXW9.
    KOiK13781.
    OMAiAFEPFQD.
    OrthoDBiEOG73BVCR.
    PhylomeDBiQ9QXW9.
    TreeFamiTF313355.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9QXW9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKGARQRNN TAKNHPGSDT SPEAEASSGG GGVALKKEIG LVSACGIIVG    50
    NIIGSGIFVS PKGVLENAGS VGLALIVWIV TGIITAVGAL CYAELGVTIP 100
    KSGGDYSYVK DIFGGLAGFL RLWIAVLVIY PTNQAVIALT FSNYVLQPLF 150
    PTCFPPESGL RLLAAICLLL LTWVNCSSVR WATRVQDIFT AGKLLALALI 200
    IIMGIVQICK GEFFWLEPKN AFENFQEPDI GLVALAFLQG SFAYGGWNFL 250
    NYVTEELVDP YKNLPRAIFI SIPLVTFVYV FANIAYVTAM SPQELLASNA 300
    VAVTFGEKLL GVMAWIMPIS VALSTFGGVN GSLFTSSRLF FAGAREGHLP 350
    SVLAMIHVKR CTPIPALLFT CLSTLLMLVT SDMYTLINYV GFINYLFYGV 400
    TVAGQIVLRW KKPDIPRPIK VSLLFPIIYL LFWAFLLIFS LWSEPVVCGI 450
    GLAIMLTGVP VYFLGVYWQH KPKCFNDFIK SLTLVSQKMC VVVYPQEGNS 500
    GAEETTDDLE EQHKPIFKPT PVKDPDSEEQ P 531
    Length:531
    Mass (Da):57,873
    Last modified:May 1, 2000 - v1
    Checksum:iAE9C3B42F3B24F8C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF171668 mRNA. Translation: AAF20380.1.
    Y19022 mRNA. Translation: CAB69072.1.
    BC059004 mRNA. Translation: AAH59004.1.
    CCDSiCCDS27101.1.
    RefSeqiNP_058668.1. NM_016972.2.
    UniGeneiMm.276831.

    Genome annotation databases

    EnsembliENSMUST00000022787; ENSMUSP00000022787; ENSMUSG00000022180.
    GeneIDi50934.
    KEGGimmu:50934.
    UCSCiuc007txa.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF171668 mRNA. Translation: AAF20380.1 .
    Y19022 mRNA. Translation: CAB69072.1 .
    BC059004 mRNA. Translation: AAH59004.1 .
    CCDSi CCDS27101.1.
    RefSeqi NP_058668.1. NM_016972.2.
    UniGenei Mm.276831.

    3D structure databases

    ProteinModelPortali Q9QXW9.
    SMRi Q9QXW9. Positions 38-368.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 206166. 1 interaction.
    STRINGi 10090.ENSMUSP00000022787.

    PTM databases

    PhosphoSitei Q9QXW9.

    Proteomic databases

    MaxQBi Q9QXW9.
    PaxDbi Q9QXW9.
    PRIDEi Q9QXW9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022787 ; ENSMUSP00000022787 ; ENSMUSG00000022180 .
    GeneIDi 50934.
    KEGGi mmu:50934.
    UCSCi uc007txa.1. mouse.

    Organism-specific databases

    CTDi 23428.
    MGIi MGI:1355323. Slc7a8.

    Phylogenomic databases

    eggNOGi COG0531.
    GeneTreei ENSGT00730000110280.
    HOGENOMi HOG000098892.
    HOVERGENi HBG000476.
    InParanoidi Q9QXW9.
    KOi K13781.
    OMAi AFEPFQD.
    OrthoDBi EOG73BVCR.
    PhylomeDBi Q9QXW9.
    TreeFami TF313355.

    Enzyme and pathway databases

    Reactomei REACT_211508. Basigin interactions.

    Miscellaneous databases

    NextBioi 307981.
    PROi Q9QXW9.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9QXW9.
    CleanExi MM_LAT2.
    Genevestigatori Q9QXW9.

    Family and domain databases

    InterProi IPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view ]
    PANTHERi PTHR11785. PTHR11785. 1 hit.
    Pfami PF13520. AA_permease_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsi TIGR00911. 2A0308. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
      Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C.
      J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: C57BL/6J.
      Tissue: Embryo.
    2. "SLC7A8, a gene mapping within the lysinuric protein intolerance critical region, encodes a new member of the glycoprotein-associated amino acid transporter family."
      Bassi M.T., Sperandeo M.P., Incerti B., Bulfone A., Pepe A., Surace E.M., Gattuso C., de Grandi A., Buoninconti A., Riboni M., Manzoni M., Andria G., Ballabio A., Borsani G., Sebastio G.
      Genomics 62:297-303(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiLAT2_MOUSE
    AccessioniPrimary (citable) accession number: Q9QXW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3