Large neutral amino acids transporter small subunit 2

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Q9QXW9 (LAT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Large neutral amino acids transporter small subunit 2

Alternative name(s):
L-type amino acid transporter 2
Short name=mLAT2
Solute carrier family 7 member 8

Gene names

Name:	Slc7a8
Synonyms:	Lat2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney. Ref.1
Subunit structure	Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Ref.1
Subcellular location	Cytoplasm By similarity. Basolateral cell membrane; Multi-pass membrane protein. Note: Localized to the cytoplasm when expressed alone By similarity. When coexpressed with SLC3A2/4F2hc, is localized to the plasma membrane. Colocalized with SLC3A2/4F2hc at the basolateral membrane of kidney cortex proximal tubules and small intestine epithelia of the villi. Ref.1
Tissue specificity	Strongly expressed in kidney and small intestine. Moderately present in placenta, ovary and brain. Ref.1
Sequence similarities	Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family. [View classification]
Biophysicochemical properties	Kinetic parameters: K_M=12.2 µM for L-phenylalanine Ref.1 K_M=48 µM for L-leucine K_M=167 µM for L-alanine K_M=275 µM for L-glutamine K_M=294 µM for L-histidine

Ontologies

Keywords
Biological process	Amino-acid transport Transport
Cellular component	Cell membrane Cytoplasm Membrane
Domain	Transmembrane Transmembrane helix
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from sequence alignment Ref.2. Source: MGI
Molecular_function	L-amino acid transmembrane transporter activity Inferred from direct assay PubMed 15659399. Source: MGI amine transmembrane transporter activity Inferred from sequence alignment Ref.2. Source: MGI organic cation transmembrane transporter activity Inferred from electronic annotation. Source: Compara toxin transporter activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 531

531

Large neutral amino acids transporter small subunit 2

PRO_0000054274

Regions

Transmembrane

39 – 59

Helical; Potential

Transmembrane

71 – 91

Helical; Potential

Transmembrane

112 – 132

Helical; Potential

Transmembrane

154 – 174

Helical; Potential

Transmembrane

188 – 208

Helical; Potential

Transmembrane

230 – 250

Helical; Potential

Transmembrane

267 – 287

Helical; Potential

Transmembrane

309 – 329

Helical; Potential

Transmembrane

361 – 381

Helical; Potential

Transmembrane

387 – 407

Helical; Potential

Transmembrane

421 – 441

Helical; Potential

Transmembrane

446 – 466

Helical; Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q9QXW9 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: AE9C3B42F3B24F8C
Show »

FASTA

531

57,873

        10         20         30         40         50         60 
MEKGARQRNN TAKNHPGSDT SPEAEASSGG GGVALKKEIG LVSACGIIVG NIIGSGIFVS 

        70         80         90        100        110        120 
PKGVLENAGS VGLALIVWIV TGIITAVGAL CYAELGVTIP KSGGDYSYVK DIFGGLAGFL 

       130        140        150        160        170        180 
RLWIAVLVIY PTNQAVIALT FSNYVLQPLF PTCFPPESGL RLLAAICLLL LTWVNCSSVR 

       190        200        210        220        230        240 
WATRVQDIFT AGKLLALALI IIMGIVQICK GEFFWLEPKN AFENFQEPDI GLVALAFLQG 

       250        260        270        280        290        300 
SFAYGGWNFL NYVTEELVDP YKNLPRAIFI SIPLVTFVYV FANIAYVTAM SPQELLASNA 

       310        320        330        340        350        360 
VAVTFGEKLL GVMAWIMPIS VALSTFGGVN GSLFTSSRLF FAGAREGHLP SVLAMIHVKR 

       370        380        390        400        410        420 
CTPIPALLFT CLSTLLMLVT SDMYTLINYV GFINYLFYGV TVAGQIVLRW KKPDIPRPIK 

       430        440        450        460        470        480 
VSLLFPIIYL LFWAFLLIFS LWSEPVVCGI GLAIMLTGVP VYFLGVYWQH KPKCFNDFIK 

       490        500        510        520        530 
SLTLVSQKMC VVVYPQEGNS GAEETTDDLE EQHKPIFKPT PVKDPDSEEQ P

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine." Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C. J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Strain: C57BL/6J. Tissue: Embryo.
[2]	"SLC7A8, a gene mapping within the lysinuric protein intolerance critical region, encodes a new member of the glycoprotein-associated amino acid transporter family." Bassi M.T., Sperandeo M.P., Incerti B., Bulfone A., Pepe A., Surace E.M., Gattuso C., de Grandi A., Buoninconti A., Riboni M., Manzoni M., Andria G., Ballabio A., Borsani G., Sebastio G. Genomics 62:297-303(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF171668 mRNA. Translation: AAF20380.1. Y19022 mRNA. Translation: CAB69072.1. BC059004 mRNA. Translation: AAH59004.1.
IPI	IPI00135632.
RefSeq	NP_058668.1. NM_016972.2.
UniGene	Mm.276831.
3D structure databases
ProteinModelPortal	Q9QXW9.
SMR	Q9QXW9. Positions 38-368.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000022787.
PTM databases
PhosphoSite	Q9QXW9.
Proteomic databases
PaxDb	Q9QXW9.
PRIDE	Q9QXW9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022787; ENSMUSP00000022787; ENSMUSG00000022180.
GeneID	50934.
KEGG	mmu:50934.
UCSC	uc007txa.1. mouse.
Organism-specific databases
CTD	23428.
MGI	MGI:1355323. Slc7a8.
Phylogenomic databases
eggNOG	COG0531.
GeneTree	ENSGT00690000101731.
HOGENOM	HOG000098892.
HOVERGEN	HBG000476.
InParanoid	Q9QXW9.
KO	K13781.
OMA	DERPAKE.
OrthoDB	EOG4V437M.
Gene expression databases
Bgee	Q9QXW9.
CleanEx	MM_LAT2.
Genevestigator	Q9QXW9.
GermOnline	ENSMUSG00000022180. Mus musculus.
Family and domain databases
InterPro	IPR002293. AA/rel_permease1. IPR004760. L_AA_transporter. [Graphical view]
PANTHER	PTHR11785. PTHR11785. 1 hit.
PIRSF	PIRSF006060. AA_transporter. 1 hit.
TIGRFAMs	TIGR00911. 2A0308. 1 hit.
ProtoNet	Search...
Other
NextBio	307981.
SOURCE	Search...

Entry information

Entry name

LAT2_MOUSE

Accession

Primary (citable) accession number: Q9QXW9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents