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Protein

Large neutral amino acids transporter small subunit 2

Gene

Slc7a8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney.1 Publication

Kineticsi

  1. KM=12.2 µM for L-phenylalanine1 Publication
  2. KM=48 µM for L-leucine1 Publication
  3. KM=167 µM for L-alanine1 Publication
  4. KM=275 µM for L-glutamine1 Publication
  5. KM=294 µM for L-histidine1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
    REACT_323105. Basigin interactions.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Large neutral amino acids transporter small subunit 2
    Alternative name(s):
    L-type amino acid transporter 2
    Short name:
    mLAT2
    Solute carrier family 7 member 8
    Gene namesi
    Name:Slc7a8
    Synonyms:Lat2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 14

    Organism-specific databases

    MGIiMGI:1355323. Slc7a8.

    Subcellular locationi

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei39 – 5921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei71 – 9121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei112 – 13221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei154 – 17421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei188 – 20821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei230 – 25021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei267 – 28721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei309 – 32921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei361 – 38121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei387 – 40721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei421 – 44121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei446 – 46621HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 531531Large neutral amino acids transporter small subunit 2PRO_0000054274Add
    BLAST

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    MaxQBiQ9QXW9.
    PaxDbiQ9QXW9.
    PRIDEiQ9QXW9.

    PTM databases

    PhosphoSiteiQ9QXW9.

    Expressioni

    Tissue specificityi

    Strongly expressed in kidney and small intestine. Moderately present in placenta, ovary and brain.1 Publication

    Gene expression databases

    BgeeiQ9QXW9.
    CleanExiMM_LAT2.
    GenevestigatoriQ9QXW9.

    Interactioni

    Subunit structurei

    Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc.1 Publication

    Protein-protein interaction databases

    BioGridi206166. 1 interaction.
    STRINGi10090.ENSMUSP00000022787.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QXW9.
    SMRiQ9QXW9. Positions 38-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0531.
    GeneTreeiENSGT00760000119037.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ9QXW9.
    KOiK13781.
    OMAiEVYGEFW.
    OrthoDBiEOG73BVCR.
    PhylomeDBiQ9QXW9.
    TreeFamiTF313355.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9QXW9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MEKGARQRNN TAKNHPGSDT SPEAEASSGG GGVALKKEIG LVSACGIIVG
    60 70 80 90 100
    NIIGSGIFVS PKGVLENAGS VGLALIVWIV TGIITAVGAL CYAELGVTIP
    110 120 130 140 150
    KSGGDYSYVK DIFGGLAGFL RLWIAVLVIY PTNQAVIALT FSNYVLQPLF
    160 170 180 190 200
    PTCFPPESGL RLLAAICLLL LTWVNCSSVR WATRVQDIFT AGKLLALALI
    210 220 230 240 250
    IIMGIVQICK GEFFWLEPKN AFENFQEPDI GLVALAFLQG SFAYGGWNFL
    260 270 280 290 300
    NYVTEELVDP YKNLPRAIFI SIPLVTFVYV FANIAYVTAM SPQELLASNA
    310 320 330 340 350
    VAVTFGEKLL GVMAWIMPIS VALSTFGGVN GSLFTSSRLF FAGAREGHLP
    360 370 380 390 400
    SVLAMIHVKR CTPIPALLFT CLSTLLMLVT SDMYTLINYV GFINYLFYGV
    410 420 430 440 450
    TVAGQIVLRW KKPDIPRPIK VSLLFPIIYL LFWAFLLIFS LWSEPVVCGI
    460 470 480 490 500
    GLAIMLTGVP VYFLGVYWQH KPKCFNDFIK SLTLVSQKMC VVVYPQEGNS
    510 520 530
    GAEETTDDLE EQHKPIFKPT PVKDPDSEEQ P
    Length:531
    Mass (Da):57,873
    Last modified:May 1, 2000 - v1
    Checksum:iAE9C3B42F3B24F8C
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF171668 mRNA. Translation: AAF20380.1.
    Y19022 mRNA. Translation: CAB69072.1.
    BC059004 mRNA. Translation: AAH59004.1.
    CCDSiCCDS27101.1.
    RefSeqiNP_058668.1. NM_016972.2.
    UniGeneiMm.276831.

    Genome annotation databases

    EnsembliENSMUST00000022787; ENSMUSP00000022787; ENSMUSG00000022180.
    GeneIDi50934.
    KEGGimmu:50934.
    UCSCiuc007txa.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF171668 mRNA. Translation: AAF20380.1.
    Y19022 mRNA. Translation: CAB69072.1.
    BC059004 mRNA. Translation: AAH59004.1.
    CCDSiCCDS27101.1.
    RefSeqiNP_058668.1. NM_016972.2.
    UniGeneiMm.276831.

    3D structure databases

    ProteinModelPortaliQ9QXW9.
    SMRiQ9QXW9. Positions 38-368.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi206166. 1 interaction.
    STRINGi10090.ENSMUSP00000022787.

    PTM databases

    PhosphoSiteiQ9QXW9.

    Proteomic databases

    MaxQBiQ9QXW9.
    PaxDbiQ9QXW9.
    PRIDEiQ9QXW9.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000022787; ENSMUSP00000022787; ENSMUSG00000022180.
    GeneIDi50934.
    KEGGimmu:50934.
    UCSCiuc007txa.1. mouse.

    Organism-specific databases

    CTDi23428.
    MGIiMGI:1355323. Slc7a8.

    Phylogenomic databases

    eggNOGiCOG0531.
    GeneTreeiENSGT00760000119037.
    HOGENOMiHOG000098892.
    HOVERGENiHBG000476.
    InParanoidiQ9QXW9.
    KOiK13781.
    OMAiEVYGEFW.
    OrthoDBiEOG73BVCR.
    PhylomeDBiQ9QXW9.
    TreeFamiTF313355.

    Enzyme and pathway databases

    ReactomeiREACT_301178. Amino acid transport across the plasma membrane.
    REACT_323105. Basigin interactions.

    Miscellaneous databases

    NextBioi307981.
    PROiQ9QXW9.
    SOURCEiSearch...

    Gene expression databases

    BgeeiQ9QXW9.
    CleanExiMM_LAT2.
    GenevestigatoriQ9QXW9.

    Family and domain databases

    InterProiIPR002293. AA/rel_permease1.
    IPR004760. L_AA_transporter.
    [Graphical view]
    PANTHERiPTHR11785. PTHR11785. 1 hit.
    PfamiPF13520. AA_permease_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006060. AA_transporter. 1 hit.
    TIGRFAMsiTIGR00911. 2A0308. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "LAT2, a new basolateral 4F2hc/CD98-associated amino acid transporter of kidney and intestine."
      Rossier G., Meier C., Bauch C., Summa V., Sordat B., Verrey F., Kuehn L.C.
      J. Biol. Chem. 274:34948-34954(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: C57BL/6J.
      Tissue: Embryo.
    2. "SLC7A8, a gene mapping within the lysinuric protein intolerance critical region, encodes a new member of the glycoprotein-associated amino acid transporter family."
      Bassi M.T., Sperandeo M.P., Incerti B., Bulfone A., Pepe A., Surace E.M., Gattuso C., de Grandi A., Buoninconti A., Riboni M., Manzoni M., Andria G., Ballabio A., Borsani G., Sebastio G.
      Genomics 62:297-303(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiLAT2_MOUSE
    AccessioniPrimary (citable) accession number: Q9QXW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: May 1, 2000
    Last modified: May 27, 2015
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.