Q9QXV8 (SPY2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein sprouty homolog 2
Short name=Spry-2

Gene names

Name:

Spry2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	315 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.
Subunit structure	Interacts with RAF1 By similarity.
Subcellular location	Cytoplasm › cytoskeleton. Cell projection › ruffle membrane. Note: Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).
Tissue specificity	In adult, highly expressed in brain, lung, heart and at lower levels in skeletal muscle and kidney. In embryo, highly expressed in lung epithelial cells, primarily in the distal airways.
Developmental stage	At E8.5 expressed in the primitive streak, rostral forebrain, cells lateral to the posterior hindbrain, anterior hindbrain and developing midbrain. At E9.5 continues to be expressed in the rostral forebrain and primitive streak, and is also detected in the branchial arches and the forelimb bud. At E10.5 expressed in the somites, frontonasal processes, tailbud, and hindlimb bud.
Induction	By FGF signaling.
Domain	The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.
Sequence similarities	Belongs to the sprouty family. Contains 1 SPR (sprouty) domain.

Ontologies

Keywords
Cellular component	Cell membrane Cell projection Cytoplasm Cytoskeleton Membrane Microtubule
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	branching morphogenesis of an epithelial tube Inferred from mutant phenotype Ref.2. Source: MGI cell fate commitment Inferred from mutant phenotype PubMed 15809037. Source: MGI inner ear morphogenesis Inferred from mutant phenotype PubMed 15809037. Source: MGI lung growth Inferred from direct assay PubMed 11287183. Source: MGI lung morphogenesis Inferred from direct assay PubMed 11287183. Source: MGI negative regulation of ERK1 and ERK2 cascade Inferred from direct assay PubMed 11585837. Source: MGI negative regulation of MAP kinase activity Inferred from direct assay PubMed 16339969. Source: MGI negative regulation of Ras GTPase activity Inferred from direct assay PubMed 11585837. Source: MGI negative regulation of Ras protein signal transduction Inferred from direct assay PubMed 11585837. Source: MGI negative regulation of apoptotic process Inferred from mutant phenotype PubMed 18070883. Source: UniProtKB negative regulation of cell proliferation Inferred from direct assay PubMed 11585837. Source: MGI negative regulation of fibroblast growth factor receptor signaling pathway Inferred from direct assay PubMed 11585837. Source: MGI negative regulation of nerve growth factor receptor signaling pathway Inferred from direct assay PubMed 11585837. Source: MGI negative regulation of peptidyl-threonine phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara positive regulation of gene expression Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-serine phosphorylation Inferred from electronic annotation. Source: Compara positive regulation of protein kinase B signaling cascade Inferred from electronic annotation. Source: Compara sensory perception of sound Inferred from mutant phenotype PubMed 15809037. Source: MGI
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW microtubule Inferred from electronic annotation. Source: UniProtKB-KW ruffle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein serine/threonine kinase activator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 315	315	Protein sprouty homolog 2		PRO_0000076902
Regions
	Domain	177 – 291	115	SPR
	Compositional bias	124 – 130	7	Poly-Ser
	Compositional bias	178 – 301	124	Cys-rich
Experimental info
	Sequence conflict	163	1	V → I in AAD34167. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9QXV8 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 81514698EAD809A7	FASTA	315	34,623
	10 20 30 40 50 60 MEARAQSGNG SQPLLQTAHD SGRQRGEPDP RDALTQQVHV LSLDQIRAIR NTNEYTEGPT 70 80 90 100 110 120 VVPRPGLKPA PRPSTQHKHE RLHGLPEHRQ PPRLQPSQVH SSRAPLSRSI STVSSGSRSS 130 140 150 160 170 180 TRTSTSSSSS EQRLLGPSFS HGPAAADGII RVQPKSELKP GDVKPLSKDD LGLHAYRCED 190 200 210 220 230 240 CGKCKCKECT YPRPLPSDWI CDKQCLCSAQ NVIDYGTCVC CVKGLFYHCS NDDEDNCADN 250 260 270 280 290 300 PCSCSQSHCC TRWSAMGVMS LFLPCLWCYL PAKGCLKLCQ GCYDRVNRPG CRCKNSNTVC 310 CKVPTVPPRN FEKPT

References

[1]	"Vertebrate sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed." Minowada G., Jarvis L.A., Chi C.L., Neubueser A., Sun X., Hacohen N., Krasnow M.A., Martin G.R. Development 126:4465-4475(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Conserved function of mSpry-2, a murine homolog of Drosophila sprouty, which negatively modulates respiratory organogenesis." Tefft J.D., Lee M., Smith S., Leinwand M., Zhao J., Bringas P. Jr., Crowe D.L., Warburton D. Curr. Biol. 9:219-222(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Swiss Webster.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF176905 mRNA. Translation: AAD56006.1. AF153084 mRNA. Translation: AAD34167.1.
IPI	IPI00135613.
RefSeq	NP_036027.1. NM_011897.3.
UniGene	Mm.489870. Mm.89982.
3D structure databases
ProteinModelPortal	Q9QXV8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9QXV8. 1 interaction.
PTM databases
PhosphoSite	Q9QXV8.
Proteomic databases
PRIDE	Q9QXV8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022709; ENSMUSP00000022709; ENSMUSG00000022114.
GeneID	24064.
KEGG	mmu:24064.
Organism-specific databases
CTD	10253.
MGI	MGI:1345138. Spry2.
Phylogenomic databases
eggNOG	NOG83905.
GeneTree	ENSGT00390000003535.
HOGENOM	HOG000233079.
HOVERGEN	HBG003544.
InParanoid	Q9QXV8.
KO	K04704.
OMA	METRAQN.
OrthoDB	EOG43TZW1.
Gene expression databases
ArrayExpress	Q9QXV8.
Bgee	Q9QXV8.
CleanEx	MM_SPRY2.
Genevestigator	Q9QXV8.
GermOnline	ENSMUSG00000022114. Mus musculus.
Family and domain databases
InterPro	IPR007875. Sprouty. [Graphical view]
Pfam	PF05210. Sprouty. 1 hit. [Graphical view]
PROSITE	PS51227. SPR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304033.
SOURCE	Search...

Entry information

Entry name

SPY2_MOUSE

Accession

Primary (citable) accession number: Q9QXV8
Secondary accession number(s): Q9WUQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program