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Protein

Protein sprouty homolog 2

Gene

Spry2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.

GO - Molecular functioni

GO - Biological processi

  • branching morphogenesis of an epithelial tube Source: MGI
  • bud elongation involved in lung branching Source: MGI
  • cell fate commitment Source: MGI
  • establishment of mitotic spindle orientation Source: MGI
  • inner ear morphogenesis Source: MGI
  • lung development Source: MGI
  • lung growth Source: MGI
  • lung morphogenesis Source: MGI
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of cell projection organization Source: BHF-UCL
  • negative regulation of cell proliferation Source: MGI
  • negative regulation of ERK1 and ERK2 cascade Source: BHF-UCL
  • negative regulation of fibroblast growth factor receptor signaling pathway Source: MGI
  • negative regulation of GTPase activity Source: MGI
  • negative regulation of MAP kinase activity Source: MGI
  • negative regulation of neurotrophin TRK receptor signaling pathway Source: MGI
  • negative regulation of peptidyl-threonine phosphorylation Source: MGI
  • negative regulation of Ras protein signal transduction Source: MGI
  • positive regulation of ERK1 and ERK2 cascade Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of peptidyl-serine phosphorylation Source: MGI
  • positive regulation of protein kinase B signaling Source: MGI
  • regulation of cell differentiation Source: InterPro
  • respiratory system development Source: MGI
  • sensory perception of sound Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Enzyme and pathway databases

ReactomeiR-MMU-1295596. Spry regulation of FGF signaling.
R-MMU-182971. EGFR downregulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein sprouty homolog 2
Short name:
Spry-2
Gene namesi
Name:Spry2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1345138. Spry2.

Subcellular locationi

GO - Cellular componenti

  • cytoskeleton Source: BHF-UCL
  • cytosol Source: BHF-UCL
  • membrane Source: BHF-UCL
  • microtubule Source: UniProtKB-KW
  • nucleus Source: BHF-UCL
  • ruffle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000769021 – 315Protein sprouty homolog 2Add BLAST315

Post-translational modificationi

Cleaved at Pro-143 by the prolyl endopeptidase FAP (seprase) activity (in vitro).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei143 – 144Cleavage; by FAPBy similarity2

Proteomic databases

MaxQBiQ9QXV8.
PaxDbiQ9QXV8.
PRIDEiQ9QXV8.

PTM databases

iPTMnetiQ9QXV8.
PhosphoSitePlusiQ9QXV8.
SwissPalmiQ9QXV8.

Expressioni

Tissue specificityi

In adult, highly expressed in brain, lung, heart and at lower levels in skeletal muscle and kidney. In embryo, highly expressed in lung epithelial cells, primarily in the distal airways.

Developmental stagei

At E8.5 expressed in the primitive streak, rostral forebrain, cells lateral to the posterior hindbrain, anterior hindbrain and developing midbrain. At E9.5 continues to be expressed in the rostral forebrain and primitive streak, and is also detected in the branchial arches and the forelimb bud. At E10.5 expressed in the somites, frontonasal processes, tailbud, and hindlimb bud.

Inductioni

By FGF signaling.

Gene expression databases

BgeeiENSMUSG00000022114.
CleanExiMM_SPRY2.
ExpressionAtlasiQ9QXV8. baseline and differential.
GenevisibleiQ9QXV8. MM.

Interactioni

Subunit structurei

Interacts with RAF1.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi204877. 11 interactors.
IntActiQ9QXV8. 1 interactor.
MINTiMINT-1591537.
STRINGi10090.ENSMUSP00000022709.

Structurei

3D structure databases

ProteinModelPortaliQ9QXV8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini177 – 291SPRPROSITE-ProRule annotationAdd BLAST115

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi124 – 130Poly-Ser7
Compositional biasi178 – 301Cys-richAdd BLAST124

Domaini

The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.

Sequence similaritiesi

Belongs to the sprouty family.Curated
Contains 1 SPR (sprouty) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IHQY. Eukaryota.
ENOG4111S05. LUCA.
GeneTreeiENSGT00390000003535.
HOGENOMiHOG000233079.
HOVERGENiHBG003544.
InParanoidiQ9QXV8.
KOiK17383.
OMAiVCCKVPS.
OrthoDBiEOG091G0DOQ.
PhylomeDBiQ9QXV8.
TreeFamiTF325070.

Family and domain databases

InterProiIPR007875. Sprouty.
IPR030780. SPRY2.
[Graphical view]
PANTHERiPTHR12365:SF8. PTHR12365:SF8. 1 hit.
PfamiPF05210. Sprouty. 1 hit.
[Graphical view]
PROSITEiPS51227. SPR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QXV8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEARAQSGNG SQPLLQTAHD SGRQRGEPDP RDALTQQVHV LSLDQIRAIR
60 70 80 90 100
NTNEYTEGPT VVPRPGLKPA PRPSTQHKHE RLHGLPEHRQ PPRLQPSQVH
110 120 130 140 150
SSRAPLSRSI STVSSGSRSS TRTSTSSSSS EQRLLGPSFS HGPAAADGII
160 170 180 190 200
RVQPKSELKP GDVKPLSKDD LGLHAYRCED CGKCKCKECT YPRPLPSDWI
210 220 230 240 250
CDKQCLCSAQ NVIDYGTCVC CVKGLFYHCS NDDEDNCADN PCSCSQSHCC
260 270 280 290 300
TRWSAMGVMS LFLPCLWCYL PAKGCLKLCQ GCYDRVNRPG CRCKNSNTVC
310
CKVPTVPPRN FEKPT
Length:315
Mass (Da):34,623
Last modified:May 1, 2000 - v1
Checksum:i81514698EAD809A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163V → I in AAD34167 (PubMed:10074434).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176905 mRNA. Translation: AAD56006.1.
AF153084 mRNA. Translation: AAD34167.1.
CCDSiCCDS27321.1.
RefSeqiNP_036027.1. NM_011897.3.
XP_006519079.1. XM_006519016.3.
UniGeneiMm.489870.
Mm.89982.

Genome annotation databases

EnsembliENSMUST00000022709; ENSMUSP00000022709; ENSMUSG00000022114.
GeneIDi24064.
KEGGimmu:24064.
UCSCiuc007uxy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF176905 mRNA. Translation: AAD56006.1.
AF153084 mRNA. Translation: AAD34167.1.
CCDSiCCDS27321.1.
RefSeqiNP_036027.1. NM_011897.3.
XP_006519079.1. XM_006519016.3.
UniGeneiMm.489870.
Mm.89982.

3D structure databases

ProteinModelPortaliQ9QXV8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204877. 11 interactors.
IntActiQ9QXV8. 1 interactor.
MINTiMINT-1591537.
STRINGi10090.ENSMUSP00000022709.

PTM databases

iPTMnetiQ9QXV8.
PhosphoSitePlusiQ9QXV8.
SwissPalmiQ9QXV8.

Proteomic databases

MaxQBiQ9QXV8.
PaxDbiQ9QXV8.
PRIDEiQ9QXV8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022709; ENSMUSP00000022709; ENSMUSG00000022114.
GeneIDi24064.
KEGGimmu:24064.
UCSCiuc007uxy.2. mouse.

Organism-specific databases

CTDi10253.
MGIiMGI:1345138. Spry2.

Phylogenomic databases

eggNOGiENOG410IHQY. Eukaryota.
ENOG4111S05. LUCA.
GeneTreeiENSGT00390000003535.
HOGENOMiHOG000233079.
HOVERGENiHBG003544.
InParanoidiQ9QXV8.
KOiK17383.
OMAiVCCKVPS.
OrthoDBiEOG091G0DOQ.
PhylomeDBiQ9QXV8.
TreeFamiTF325070.

Enzyme and pathway databases

ReactomeiR-MMU-1295596. Spry regulation of FGF signaling.
R-MMU-182971. EGFR downregulation.

Miscellaneous databases

PROiQ9QXV8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022114.
CleanExiMM_SPRY2.
ExpressionAtlasiQ9QXV8. baseline and differential.
GenevisibleiQ9QXV8. MM.

Family and domain databases

InterProiIPR007875. Sprouty.
IPR030780. SPRY2.
[Graphical view]
PANTHERiPTHR12365:SF8. PTHR12365:SF8. 1 hit.
PfamiPF05210. Sprouty. 1 hit.
[Graphical view]
PROSITEiPS51227. SPR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPY2_MOUSE
AccessioniPrimary (citable) accession number: Q9QXV8
Secondary accession number(s): Q9WUQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.