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Q9QXV8 (SPY2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein sprouty homolog 2

Short name=Spry-2
Gene names
Name:Spry2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.

Subunit structure

Interacts with RAF1 By similarity.

Subcellular location

Cytoplasmcytoskeleton. Cell projectionruffle membrane. Note: Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).

Tissue specificity

In adult, highly expressed in brain, lung, heart and at lower levels in skeletal muscle and kidney. In embryo, highly expressed in lung epithelial cells, primarily in the distal airways.

Developmental stage

At E8.5 expressed in the primitive streak, rostral forebrain, cells lateral to the posterior hindbrain, anterior hindbrain and developing midbrain. At E9.5 continues to be expressed in the rostral forebrain and primitive streak, and is also detected in the branchial arches and the forelimb bud. At E10.5 expressed in the somites, frontonasal processes, tailbud, and hindlimb bud.

Induction

By FGF signaling.

Domain

The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.

Sequence similarities

Belongs to the sprouty family.

Contains 1 SPR (sprouty) domain.

Ontologies

Keywords
   Cellular componentCell membrane
Cell projection
Cytoplasm
Cytoskeleton
Membrane
Microtubule
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processbranching morphogenesis of an epithelial tube

Inferred from mutant phenotype Ref.2. Source: MGI

bud elongation involved in lung branching

Inferred from genetic interaction PubMed 21764747. Source: MGI

cell fate commitment

Inferred from mutant phenotype PubMed 15809037. Source: MGI

establishment of mitotic spindle orientation

Inferred from genetic interaction PubMed 21764747. Source: MGI

inner ear morphogenesis

Inferred from mutant phenotype PubMed 15809037. Source: MGI

lung development

Inferred from mutant phenotype Ref.2. Source: MGI

lung growth

Inferred from direct assay PubMed 11287183. Source: MGI

lung morphogenesis

Inferred from direct assay PubMed 11287183. Source: MGI

negative regulation of ERK1 and ERK2 cascade

Inferred from direct assay PubMed 11585837. Source: MGI

negative regulation of MAP kinase activity

Inferred from direct assay PubMed 16339969. Source: MGI

negative regulation of Ras GTPase activity

Inferred from direct assay PubMed 11585837. Source: MGI

negative regulation of Ras protein signal transduction

Inferred from direct assay PubMed 11585837. Source: MGI

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 18070883. Source: UniProtKB

negative regulation of cell proliferation

Inferred from direct assay PubMed 11585837. Source: MGI

negative regulation of fibroblast growth factor receptor signaling pathway

Inferred from direct assay PubMed 11585837. Source: MGI

negative regulation of neurotrophin TRK receptor signaling pathway

Inferred from direct assay PubMed 11585837. Source: MGI

negative regulation of peptidyl-threonine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of ERK1 and ERK2 cascade

Inferred from electronic annotation. Source: Ensembl

positive regulation of gene expression

Inferred from electronic annotation. Source: Ensembl

positive regulation of peptidyl-serine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of protein kinase B signaling

Inferred from electronic annotation. Source: Ensembl

respiratory system development

Inferred from direct assay PubMed 11287183. Source: MGI

sensory perception of sound

Inferred from mutant phenotype PubMed 15809037. Source: MGI

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule

Inferred from electronic annotation. Source: UniProtKB-KW

ruffle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 11585837PubMed 16339969. Source: MGI

protein serine/threonine kinase activator activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 315315Protein sprouty homolog 2
PRO_0000076902

Regions

Domain177 – 291115SPR
Compositional bias124 – 1307Poly-Ser
Compositional bias178 – 301124Cys-rich

Experimental info

Sequence conflict1631V → I in AAD34167. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9QXV8 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 81514698EAD809A7

FASTA31534,623
        10         20         30         40         50         60 
MEARAQSGNG SQPLLQTAHD SGRQRGEPDP RDALTQQVHV LSLDQIRAIR NTNEYTEGPT 

        70         80         90        100        110        120 
VVPRPGLKPA PRPSTQHKHE RLHGLPEHRQ PPRLQPSQVH SSRAPLSRSI STVSSGSRSS 

       130        140        150        160        170        180 
TRTSTSSSSS EQRLLGPSFS HGPAAADGII RVQPKSELKP GDVKPLSKDD LGLHAYRCED 

       190        200        210        220        230        240 
CGKCKCKECT YPRPLPSDWI CDKQCLCSAQ NVIDYGTCVC CVKGLFYHCS NDDEDNCADN 

       250        260        270        280        290        300 
PCSCSQSHCC TRWSAMGVMS LFLPCLWCYL PAKGCLKLCQ GCYDRVNRPG CRCKNSNTVC 

       310 
CKVPTVPPRN FEKPT 

« Hide

References

[1]"Vertebrate sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed."
Minowada G., Jarvis L.A., Chi C.L., Neubueser A., Sun X., Hacohen N., Krasnow M.A., Martin G.R.
Development 126:4465-4475(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Conserved function of mSpry-2, a murine homolog of Drosophila sprouty, which negatively modulates respiratory organogenesis."
Tefft J.D., Lee M., Smith S., Leinwand M., Zhao J., Bringas P. Jr., Crowe D.L., Warburton D.
Curr. Biol. 9:219-222(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss Webster.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF176905 mRNA. Translation: AAD56006.1.
AF153084 mRNA. Translation: AAD34167.1.
CCDSCCDS27321.1.
RefSeqNP_036027.1. NM_011897.3.
XP_006519079.1. XM_006519016.1.
UniGeneMm.489870.
Mm.89982.

3D structure databases

ProteinModelPortalQ9QXV8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204877. 11 interactions.
IntActQ9QXV8. 1 interaction.
MINTMINT-1591537.

PTM databases

PhosphoSiteQ9QXV8.

Proteomic databases

PRIDEQ9QXV8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022709; ENSMUSP00000022709; ENSMUSG00000022114.
GeneID24064.
KEGGmmu:24064.
UCSCuc007uxy.2. mouse.

Organism-specific databases

CTD10253.
MGIMGI:1345138. Spry2.

Phylogenomic databases

eggNOGNOG83905.
GeneTreeENSGT00390000003535.
HOGENOMHOG000233079.
HOVERGENHBG003544.
InParanoidQ9QXV8.
KOK17383.
OMAPHGEPDL.
OrthoDBEOG7G4QDV.
PhylomeDBQ9QXV8.
TreeFamTF325070.

Gene expression databases

ArrayExpressQ9QXV8.
BgeeQ9QXV8.
CleanExMM_SPRY2.
GenevestigatorQ9QXV8.

Family and domain databases

InterProIPR007875. Sprouty.
[Graphical view]
PfamPF05210. Sprouty. 1 hit.
[Graphical view]
PROSITEPS51227. SPR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio304033.
PROQ9QXV8.
SOURCESearch...

Entry information

Entry nameSPY2_MOUSE
AccessionPrimary (citable) accession number: Q9QXV8
Secondary accession number(s): Q9WUQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot