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Q9QXV8

- SPY2_MOUSE

UniProt

Q9QXV8 - SPY2_MOUSE

Protein

Protein sprouty homolog 2

Gene

Spry2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    May function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis.

    GO - Molecular functioni

    1. protein binding Source: MGI
    2. protein serine/threonine kinase activator activity Source: Ensembl

    GO - Biological processi

    1. branching morphogenesis of an epithelial tube Source: MGI
    2. bud elongation involved in lung branching Source: MGI
    3. cell fate commitment Source: MGI
    4. establishment of mitotic spindle orientation Source: MGI
    5. inner ear morphogenesis Source: MGI
    6. lung development Source: MGI
    7. lung growth Source: MGI
    8. lung morphogenesis Source: MGI
    9. negative regulation of apoptotic process Source: UniProtKB
    10. negative regulation of cell proliferation Source: MGI
    11. negative regulation of ERK1 and ERK2 cascade Source: MGI
    12. negative regulation of fibroblast growth factor receptor signaling pathway Source: MGI
    13. negative regulation of MAP kinase activity Source: MGI
    14. negative regulation of neurotrophin TRK receptor signaling pathway Source: MGI
    15. negative regulation of peptidyl-threonine phosphorylation Source: Ensembl
    16. negative regulation of Ras GTPase activity Source: MGI
    17. negative regulation of Ras protein signal transduction Source: MGI
    18. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
    19. positive regulation of gene expression Source: Ensembl
    20. positive regulation of peptidyl-serine phosphorylation Source: Ensembl
    21. positive regulation of protein kinase B signaling Source: Ensembl
    22. respiratory system development Source: MGI
    23. sensory perception of sound Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Enzyme and pathway databases

    ReactomeiREACT_198526. Spry regulation of FGF signaling.
    REACT_203917. EGFR downregulation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein sprouty homolog 2
    Short name:
    Spry-2
    Gene namesi
    Name:Spry2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:1345138. Spry2.

    Subcellular locationi

    Cytoplasmcytoskeleton. Cell projectionruffle membrane
    Note: Associated with microtubules in unstimulated cells but is translocated to the membrane ruffles in cells stimulated ith EGF (epidermal growth factor).

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. microtubule Source: UniProtKB-KW
    3. ruffle membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 315315Protein sprouty homolog 2PRO_0000076902Add
    BLAST

    Proteomic databases

    PRIDEiQ9QXV8.

    PTM databases

    PhosphoSiteiQ9QXV8.

    Expressioni

    Tissue specificityi

    In adult, highly expressed in brain, lung, heart and at lower levels in skeletal muscle and kidney. In embryo, highly expressed in lung epithelial cells, primarily in the distal airways.

    Developmental stagei

    At E8.5 expressed in the primitive streak, rostral forebrain, cells lateral to the posterior hindbrain, anterior hindbrain and developing midbrain. At E9.5 continues to be expressed in the rostral forebrain and primitive streak, and is also detected in the branchial arches and the forelimb bud. At E10.5 expressed in the somites, frontonasal processes, tailbud, and hindlimb bud.

    Inductioni

    By FGF signaling.

    Gene expression databases

    ArrayExpressiQ9QXV8.
    BgeeiQ9QXV8.
    CleanExiMM_SPRY2.
    GenevestigatoriQ9QXV8.

    Interactioni

    Subunit structurei

    Interacts with RAF1.By similarity

    Protein-protein interaction databases

    BioGridi204877. 11 interactions.
    IntActiQ9QXV8. 1 interaction.
    MINTiMINT-1591537.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9QXV8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini177 – 291115SPRPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi124 – 1307Poly-Ser
    Compositional biasi178 – 301124Cys-richAdd
    BLAST

    Domaini

    The Cys-rich domain is responsible for the localization of the protein to the membrane ruffles.

    Sequence similaritiesi

    Belongs to the sprouty family.Curated
    Contains 1 SPR (sprouty) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG83905.
    GeneTreeiENSGT00390000003535.
    HOGENOMiHOG000233079.
    HOVERGENiHBG003544.
    InParanoidiQ9QXV8.
    KOiK17383.
    OMAiPHGEPDL.
    OrthoDBiEOG7G4QDV.
    PhylomeDBiQ9QXV8.
    TreeFamiTF325070.

    Family and domain databases

    InterProiIPR007875. Sprouty.
    [Graphical view]
    PfamiPF05210. Sprouty. 1 hit.
    [Graphical view]
    PROSITEiPS51227. SPR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9QXV8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEARAQSGNG SQPLLQTAHD SGRQRGEPDP RDALTQQVHV LSLDQIRAIR    50
    NTNEYTEGPT VVPRPGLKPA PRPSTQHKHE RLHGLPEHRQ PPRLQPSQVH 100
    SSRAPLSRSI STVSSGSRSS TRTSTSSSSS EQRLLGPSFS HGPAAADGII 150
    RVQPKSELKP GDVKPLSKDD LGLHAYRCED CGKCKCKECT YPRPLPSDWI 200
    CDKQCLCSAQ NVIDYGTCVC CVKGLFYHCS NDDEDNCADN PCSCSQSHCC 250
    TRWSAMGVMS LFLPCLWCYL PAKGCLKLCQ GCYDRVNRPG CRCKNSNTVC 300
    CKVPTVPPRN FEKPT 315
    Length:315
    Mass (Da):34,623
    Last modified:May 1, 2000 - v1
    Checksum:i81514698EAD809A7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti163 – 1631V → I in AAD34167. (PubMed:10074434)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF176905 mRNA. Translation: AAD56006.1.
    AF153084 mRNA. Translation: AAD34167.1.
    CCDSiCCDS27321.1.
    RefSeqiNP_036027.1. NM_011897.3.
    XP_006519079.1. XM_006519016.1.
    UniGeneiMm.489870.
    Mm.89982.

    Genome annotation databases

    EnsembliENSMUST00000022709; ENSMUSP00000022709; ENSMUSG00000022114.
    GeneIDi24064.
    KEGGimmu:24064.
    UCSCiuc007uxy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF176905 mRNA. Translation: AAD56006.1 .
    AF153084 mRNA. Translation: AAD34167.1 .
    CCDSi CCDS27321.1.
    RefSeqi NP_036027.1. NM_011897.3.
    XP_006519079.1. XM_006519016.1.
    UniGenei Mm.489870.
    Mm.89982.

    3D structure databases

    ProteinModelPortali Q9QXV8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204877. 11 interactions.
    IntActi Q9QXV8. 1 interaction.
    MINTi MINT-1591537.

    PTM databases

    PhosphoSitei Q9QXV8.

    Proteomic databases

    PRIDEi Q9QXV8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022709 ; ENSMUSP00000022709 ; ENSMUSG00000022114 .
    GeneIDi 24064.
    KEGGi mmu:24064.
    UCSCi uc007uxy.2. mouse.

    Organism-specific databases

    CTDi 10253.
    MGIi MGI:1345138. Spry2.

    Phylogenomic databases

    eggNOGi NOG83905.
    GeneTreei ENSGT00390000003535.
    HOGENOMi HOG000233079.
    HOVERGENi HBG003544.
    InParanoidi Q9QXV8.
    KOi K17383.
    OMAi PHGEPDL.
    OrthoDBi EOG7G4QDV.
    PhylomeDBi Q9QXV8.
    TreeFami TF325070.

    Enzyme and pathway databases

    Reactomei REACT_198526. Spry regulation of FGF signaling.
    REACT_203917. EGFR downregulation.

    Miscellaneous databases

    NextBioi 304033.
    PROi Q9QXV8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9QXV8.
    Bgeei Q9QXV8.
    CleanExi MM_SPRY2.
    Genevestigatori Q9QXV8.

    Family and domain databases

    InterProi IPR007875. Sprouty.
    [Graphical view ]
    Pfami PF05210. Sprouty. 1 hit.
    [Graphical view ]
    PROSITEi PS51227. SPR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Vertebrate sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed."
      Minowada G., Jarvis L.A., Chi C.L., Neubueser A., Sun X., Hacohen N., Krasnow M.A., Martin G.R.
      Development 126:4465-4475(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Conserved function of mSpry-2, a murine homolog of Drosophila sprouty, which negatively modulates respiratory organogenesis."
      Tefft J.D., Lee M., Smith S., Leinwand M., Zhao J., Bringas P. Jr., Crowe D.L., Warburton D.
      Curr. Biol. 9:219-222(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss Webster.

    Entry informationi

    Entry nameiSPY2_MOUSE
    AccessioniPrimary (citable) accession number: Q9QXV8
    Secondary accession number(s): Q9WUQ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3