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Protein

Chromobox protein homolog 8

Gene

Cbx8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (By similarity).By similarity

GO - Molecular functioni

  • methylated histone binding Source: MGI
  • single-stranded RNA binding Source: UniProtKB
  • ubiquitin-protein transferase activator activity Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-2559580. Oxidative Stress Induced Senescence.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-4570464. SUMOylation of RNA binding proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 8
Alternative name(s):
Polycomb 3 homolog
Short name:
Pc3
Short name:
mPc3
Gene namesi
Name:Cbx8
Synonyms:Pc3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1353589. Cbx8.

Subcellular locationi

GO - Cellular componenti

  • heterochromatin Source: UniProtKB
  • nuclear chromatin Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • PcG protein complex Source: MGI
  • PRC1 complex Source: UniProtKB
  • ubiquitin ligase complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 362362Chromobox protein homolog 8PRO_0000080216Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei110 – 1101PhosphoserineCombined sources
Modified residuei164 – 1641PhosphoserineBy similarity
Modified residuei229 – 2291PhosphoserineCombined sources
Modified residuei234 – 2341PhosphotyrosineCombined sources
Modified residuei238 – 2381PhosphoserineBy similarity
Modified residuei284 – 2841PhosphoserineCombined sources
Modified residuei305 – 3051PhosphoserineBy similarity
Modified residuei325 – 3251PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9QXV1.
MaxQBiQ9QXV1.
PaxDbiQ9QXV1.
PeptideAtlasiQ9QXV1.
PRIDEiQ9QXV1.

PTM databases

iPTMnetiQ9QXV1.
PhosphoSiteiQ9QXV1.

Expressioni

Gene expression databases

BgeeiQ9QXV1.
CleanExiMM_CBX8.
GenevisibleiQ9QXV1. MM.

Interactioni

Subunit structurei

Component of a PRC1-like complex. Interacts with RING1, RNF2, PCGF1, PCGF2, PCGF3, BMI1, PCGF5, PCGF6 and PHC2. Interacts with histone H3 (By similarity). Interacts with MLLT3. Interacts with PHC2.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Bmi1P259163EBI-1216641,EBI-927401
Ring1O357302EBI-1216641,EBI-929310
Rnf2Q9CQJ45EBI-1216641,EBI-927321

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206027. 7 interactions.
IntActiQ9QXV1. 5 interactions.
STRINGi10090.ENSMUSP00000026663.

Structurei

Secondary structure

1
362
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 3015Combined sources
Beta strandi42 – 443Combined sources
Turni45 – 473Combined sources
Helixi51 – 588Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DNVNMR-A8-58[»]
ProteinModelPortaliQ9QXV1.
SMRiQ9QXV1. Positions 9-60.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QXV1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 6959ChromoPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 chromo domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IPQ7. Eukaryota.
ENOG410XRRX. LUCA.
GeneTreeiENSGT00530000063056.
HOGENOMiHOG000233642.
HOVERGENiHBG003608.
InParanoidiQ9QXV1.
KOiK11455.
OMAiTGKFPAG.
OrthoDBiEOG77T15B.
PhylomeDBiQ9QXV1.
TreeFamiTF106456.

Family and domain databases

InterProiIPR000953. Chromo/shadow_dom.
IPR023780. Chromo_domain.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamiPF00385. Chromo. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
PROSITEiPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QXV1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSAVGERV FAAEALLKRR IRKGRMEYLV KWKGWSQKYS TWEPEENILD
60 70 80 90 100
ARLLAAFEER EREMELYGPK KRGPKPKTFL LKAQAKAKAK TYEFRSDSTR
110 120 130 140 150
GIRIPYPGRS PQDLASTSRA REGLRNTGLP PPGSSTSTCR ADPPRDRDRE
160 170 180 190 200
RDRGTSRVDD KPSSPGDSSK KRGPKPRKEP LDPSQRPLGE PSAGLGEYLK
210 220 230 240 250
GRKLDETSSG TGKFPAGHSV IQLARRQDSD LVQYGVTSPS SAEASSKLAV
260 270 280 290 300
DTFPARVIKH RAAFLEAKGQ GALDPGGARV RHSSGTPASV GSLYRDMGAQ
310 320 330 340 350
GGRPSLIARI PVARILGDPE EESWSPSLTN LEKVVVTDVT SNFLTVTIKE
360
SNTDQGFFKE KR
Length:362
Mass (Da):39,860
Last modified:May 1, 2000 - v1
Checksum:iEE047F57483741B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180370 mRNA. Translation: AAF25615.1.
AF202116 Genomic DNA. Translation: AAF26713.1.
BC014815 mRNA. Translation: AAH14815.1.
CCDSiCCDS25709.1.
RefSeqiNP_038954.1. NM_013926.1.
UniGeneiMm.99953.

Genome annotation databases

EnsembliENSMUST00000026663; ENSMUSP00000026663; ENSMUSG00000025578.
GeneIDi30951.
KEGGimmu:30951.
UCSCiuc007mpu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF180370 mRNA. Translation: AAF25615.1.
AF202116 Genomic DNA. Translation: AAF26713.1.
BC014815 mRNA. Translation: AAH14815.1.
CCDSiCCDS25709.1.
RefSeqiNP_038954.1. NM_013926.1.
UniGeneiMm.99953.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DNVNMR-A8-58[»]
ProteinModelPortaliQ9QXV1.
SMRiQ9QXV1. Positions 9-60.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206027. 7 interactions.
IntActiQ9QXV1. 5 interactions.
STRINGi10090.ENSMUSP00000026663.

PTM databases

iPTMnetiQ9QXV1.
PhosphoSiteiQ9QXV1.

Proteomic databases

EPDiQ9QXV1.
MaxQBiQ9QXV1.
PaxDbiQ9QXV1.
PeptideAtlasiQ9QXV1.
PRIDEiQ9QXV1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026663; ENSMUSP00000026663; ENSMUSG00000025578.
GeneIDi30951.
KEGGimmu:30951.
UCSCiuc007mpu.1. mouse.

Organism-specific databases

CTDi57332.
MGIiMGI:1353589. Cbx8.

Phylogenomic databases

eggNOGiENOG410IPQ7. Eukaryota.
ENOG410XRRX. LUCA.
GeneTreeiENSGT00530000063056.
HOGENOMiHOG000233642.
HOVERGENiHBG003608.
InParanoidiQ9QXV1.
KOiK11455.
OMAiTGKFPAG.
OrthoDBiEOG77T15B.
PhylomeDBiQ9QXV1.
TreeFamiTF106456.

Enzyme and pathway databases

ReactomeiR-MMU-2559580. Oxidative Stress Induced Senescence.
R-MMU-3108214. SUMOylation of DNA damage response and repair proteins.
R-MMU-4570464. SUMOylation of RNA binding proteins.

Miscellaneous databases

EvolutionaryTraceiQ9QXV1.
PROiQ9QXV1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QXV1.
CleanExiMM_CBX8.
GenevisibleiQ9QXV1. MM.

Family and domain databases

InterProiIPR000953. Chromo/shadow_dom.
IPR023780. Chromo_domain.
IPR016197. Chromodomain-like.
IPR023779. Chromodomain_CS.
[Graphical view]
PfamiPF00385. Chromo. 1 hit.
[Graphical view]
SMARTiSM00298. CHROMO. 1 hit.
[Graphical view]
SUPFAMiSSF54160. SSF54160. 1 hit.
PROSITEiPS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and analysis of a third mouse Polycomb gene, MPc3."
    Hemenway C.S., Halligan B.W., Gould G.C.D., Levy L.S.
    Gene 242:31-40(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: 129/SvJ.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. "Role of Bmi-1 and Ring1A in H2A ubiquitylation and Hox gene silencing."
    Cao R., Tsukada Y., Zhang Y.
    Mol. Cell 20:845-854(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: RECONSTITUTION OF A PRC1-LIKE COMPLEX, INTERACTION WITH RING1; RNF2; BMI1 AND PHC2.
  4. "The polycomb protein MPc3 interacts with AF9, an MLL fusion partner in t(9;11)(p22;q23) acute leukemias."
    Hemenway C.S., de Erkenez A.C., Gould G.C.D.
    Oncogene 20:3798-3805(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MLLT3.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-229 AND SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-110; SER-229; TYR-234 AND SER-284, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Lung, Pancreas and Spleen.
  7. "Solution structure of RSGI RUH-055, a chromo domain from Mus musculus cDNA."
    RIKEN structural genomics initiative (RSGI)
    Submitted (OCT-2006) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 7-58.

Entry informationi

Entry nameiCBX8_MOUSE
AccessioniPrimary (citable) accession number: Q9QXV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 1, 2000
Last modified: July 6, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.