Q9QXU7 (PROK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Prokineticin-2
Short name=PK2

Alternative name(s):
Protein Bv8 homolog

Gene names

Name:	Prok2
Synonyms:	Bv8

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	128 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function as an output molecule from the suprachiasmatic nucleus (SCN) that transmits behavioral circadian rhythm. May also function locally within the SCN to synchronize output. Potently contracts gastrointestinal (GI) smooth muscle By similarity. Ref.3
Subcellular location	Secreted.
Tissue specificity	Expressed in the SCN and among a few other discrete brain areas, including the islands of Calleja, media l preoptic area of the hypothalamus and the shell of the nucleus accumbens. Highly expressed in testis. In the SCN, expression subjected to high amplitude of circadian oscillation.
Developmental stage	Expressed in mid-late pachytene spermatocytes at the stages VII, VIII and IX of the semiferous epithelial cycle.
Induction	Activated by CLOCK and BMAL1 heterodimers and light; inhibited by period genes (PER1, PER2 and PER3) and cryptochrome genes (CRY1 and CRY2).
Sequence similarities	Belongs to the AVIT (prokineticin) family.

Ontologies

Keywords
Biological process	Biological rhythms
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Molecular function	Neuropeptide
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	angiogenesis Inferred from sequence or structural similarity. Source: UniProtKB cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB chemotaxis Inferred from sequence or structural similarity. Source: UniProtKB circadian rhythm Inferred from direct assay Ref.3. Source: MGI epicardium-derived cardiac endothelial cell differentiation Traceable author statement PubMed 20299672. Source: DFLAT epicardium-derived cardiac vascular smooth muscle cell differentiation Traceable author statement PubMed 20299672. Source: DFLAT negative regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of smooth muscle contraction Inferred from sequence or structural similarity. Source: UniProtKB spermatogenesis Inferred from sequence or structural similarity. Source: UniProtKB vasculogenesis involved in coronary vascular morphogenesis Traceable author statement PubMed 20299672. Source: DFLAT
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor binding Traceable author statement PubMed 20299672. Source: DFLAT
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q9QXU7-1)

Also known as: Bv8-a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q9QXU7-2)

Also known as: Bv8-b;

The sequence of this isoform differs from the canonical sequence as follows:
     74-94: Missing.

Isoform 3 (identifier: Q9QXU7-3)

The sequence of this isoform differs from the canonical sequence as follows:
     74-128: SHVANGRQERRRAKRRKRKKEVPFWGRRMHHTCPCLPGLACLRTSFNRFICLARK → VSVCTGILGVPSH

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 128	102	Prokineticin-2		PRO_0000025810
Amino acid modifications
	Disulfide bond	33 ↔ 45		By similarity
	Disulfide bond	39 ↔ 57		By similarity
	Disulfide bond	44 ↔ 106		By similarity
	Disulfide bond	67 ↔ 114		By similarity
	Disulfide bond	108 ↔ 124		By similarity
Natural variations
	Alternative sequence	74 – 128	55	SHVAN…CLARK → VSVCTGILGVPSH in isoform 3.		VSP_005221
	Alternative sequence	74 – 94	21	Missing in isoform 2.		VSP_005220

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Bv8-a) [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 5F08BA177FDDB58C	FASTA	128	14,185
	10 20 30 40 50 60 MGDPRCAPLL LLLLLPLLFT PPAGDAAVIT GACDKDSQCG GGMCCAVSIW VKSIRICTPM 70 80 90 100 110 120 GQVGDSCHPL TRKSHVANGR QERRRAKRRK RKKEVPFWGR RMHHTCPCLP GLACLRTSFN RFICLARK
	Isoform 2 (Bv8-b) [UniParc]. Checksum: 8FB79BE4D4357C70	FASTA	107	11,556
	10 20 30 40 50 60 MGDPRCAPLL LLLLLPLLFT PPAGDAAVIT GACDKDSQCG GGMCCAVSIW VKSIRICTPM 70 80 90 100 GQVGDSCHPL TRKVPFWGRR MHHTCPCLPG LACLRTSFNR FICLARK
	Isoform 3 [UniParc]. Checksum: 28EACBC5E6F7706F	FASTA	86	8,836
	10 20 30 40 50 60 MGDPRCAPLL LLLLLPLLFT PPAGDAAVIT GACDKDSQCG GGMCCAVSIW VKSIRICTPM 70 80 GQVGDSCHPL TRKVSVCTGI LGVPSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mammalian homologues of frog Bv8 are mainly expressed in spermatocytes." Wechselberger C., Puglisi R., Lepperdinger G., Boitani C., Kreil G. FEBS Lett. 462:177-181(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2). Strain: 129/Sv.
[2]	"Murine Bv8 gene maps near a synteny breakpoint of mouse chromosome 6 and human 3p21." Jilek A., Engel E., Beier D., Lepperdinger G. Gene 256:189-195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2 AND 3). Strain: 129/SvJ.
[3]	"Prokineticin 2 transmits the behavioural circadian rhythm of the suprachiasmatic nucleus." Cheng M.Y., Bullock C.M., Li C., Lee A.G., Bermak J.C., Belluzzi J., Weaver D.R., Leslie F.M., Zhou Q.-Y. Nature 417:405-410(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION. Strain: C57BL/6.
[4]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: C57BL/6J. Tissue: Testis.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF182064 mRNA. Translation: AAF15259.1. AF182065 mRNA. Translation: AAF15260.1. AF182066 mRNA. Translation: AAF15261.1. AF182068, AF182067 Genomic DNA. Translation: AAG09439.1. AF487280 mRNA. Translation: AAM49572.1. AK015462 mRNA. Translation: BAB29857.1. BC144863 mRNA. Translation: AAI44864.1.
IPI	IPI00135577. IPI00230605. IPI00230606.
RefSeq	NP_001032628.1. NM_001037539.2. NP_056583.1. NM_015768.2.
UniGene	Mm.87365.
3D structure databases
ProteinModelPortal	Q9QXU7.
SMR	Q9QXU7. Positions 27-127.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000032152.
Proteomic databases
PRIDE	Q9QXU7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032152; ENSMUSP00000032152; ENSMUSG00000030069. ENSMUST00000101120; ENSMUSP00000098678; ENSMUSG00000030069.
GeneID	50501.
KEGG	mmu:50501.
UCSC	uc009dbu.1. mouse. uc009dbv.1. mouse.
Organism-specific databases
CTD	60675.
MGI	MGI:1354178. Prok2.
Phylogenomic databases
eggNOG	NOG39081.
GeneTree	ENSGT00390000014799.
HOGENOM	HOG000004848.
HOVERGEN	HBG031845.
InParanoid	Q9QXU7.
OMA	PLTRKNH.
OrthoDB	EOG42NJ21.
Gene expression databases
ArrayExpress	Q9QXU7.
Bgee	Q9QXU7.
CleanEx	MM_PROK2.
Genevestigator	Q9QXU7.
GermOnline	ENSMUSG00000030069. Mus musculus.
Family and domain databases
InterPro	IPR009523. Prokineticin. IPR023569. Prokineticin_domain. [Graphical view]
PANTHER	PTHR18821. PTHR18821. 1 hit.
Pfam	PF06607. Prokineticin. 1 hit. [Graphical view]
ProDom	PD059788. Prokineticin. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...
Other
NextBio	307502.
SOURCE	Search...

Entry information

Entry name

PROK2_MOUSE

Accession

Primary (citable) accession number: Q9QXU7
Secondary accession number(s): B7ZMX7, Q9QXU5, Q9QXU6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program