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Q9QXT5 (EGFL7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Epidermal growth factor-like protein 7

Short name=EGF-like protein 7
Alternative name(s):
Multiple epidermal growth factor-like domains protein 7
Short name=Multiple EGF-like domains protein 7
NOTCH4-like protein
Vascular endothelial statin
Short name=VE-statin
Zneu1
Gene names
Name:Egfl7
Synonyms:Megf7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length275 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Regulates vascular tubulogenesis in vivo. Inhibits platelet-derived growth factor (PDGF)-BB-induced smooth muscle cell migration and promotes endothelial cell adhesion to the extracellular matrix and angiogenesis. Ref.1 Ref.6

Subunit structure

Interacts with ITGAV/ITGB3 in an RGD-dependent manner, increasing endothelial cell's motility By similarity.

Subcellular location

Secretedextracellular space Ref.1 Ref.2.

Tissue specificity

Expressed specifically by endothelial cells of the highly vascularized organs heart, lung and kidney. Ref.1 Ref.2

Developmental stage

Expressed early during mouse embryogenesis in the yolk sac mesoderm and in the developing vascular system. At E7.5, it is expressed in the primitive blood islands where the first endothelial cells differentiate. At E10.5 and E13.5 expression is restricted to endothelial cells. Ref.1 Ref.2

Sequence similarities

Contains 2 EGF-like domains.

Contains 1 EMI domain.

Sequence caution

The sequence AAF01322.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAP74732.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAB22222.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9QXT5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9QXT5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     257-269: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 275254Epidermal growth factor-like protein 7
PRO_0000007529

Regions

Domain28 – 10578EMI
Domain104 – 13633EGF-like 1
Domain138 – 17841EGF-like 2; calcium-binding Potential
Coiled coil196 – 22025 Potential
Motif131 – 1333Cell attachment site By similarity

Amino acid modifications

Disulfide bond32 ↔ 90 By similarity
Disulfide bond57 ↔ 63 By similarity
Disulfide bond89 ↔ 103 By similarity
Disulfide bond108 ↔ 118 By similarity
Disulfide bond112 ↔ 124 By similarity
Disulfide bond126 ↔ 135 By similarity
Disulfide bond142 ↔ 153 By similarity
Disulfide bond149 ↔ 162 By similarity

Natural variations

Alternative sequence257 – 26913Missing in isoform 2.
VSP_011765

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 4, 2005. Version 2.
Checksum: 54A888CB51CFAA13

FASTA27529,765
        10         20         30         40         50         60 
MWGSGELLVA WFLVLAADGT TEHVYRPSRR VCTVGISGGS ISETFVQRVY QPYLTTCDGH 

        70         80         90        100        110        120 
RACSTYRTIY RTAYRRSPGV TPARPRYACC PGWKRTSGLP GACGAAICQP PCGNGGSCIR 

       130        140        150        160        170        180 
PGHCRCPVGW QGDTCQTDVD ECSTGEASCP QRCVNTVGSY WCQGWEGQSP SADGTRCLSK 

       190        200        210        220        230        240 
EGPSPVAPNP TAGVDSMARE EVYRLQARVD VLEQKLQLVL APLHSLASRS TEHGLQDPGS 

       250        260        270 
LLAHSFQQLD RIDSLSEQVS FLEEHLGSCS CKKDL 

« Hide

Isoform 2 [UniParc].

Checksum: 7AB272F68CB7D4A4
Show »

FASTA26228,305

References

« Hide 'large scale' references
[1]"VE-statin, an endothelial repressor of smooth muscle cell migration."
Soncin F., Mattot V., Lionneton F., Spruyt N., Lepretre F., Begue A., Stehelin D.
EMBO J. 22:5700-5711(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: B6SJL.
[2]"Egfl7, a novel epidermal growth factor-domain gene expressed in endothelial cells."
Fitch M.J., Campagnolo L., Kuhnert F., Stuhlmann H.
Dev. Dyn. 230:316-324(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: CD-1.
[3]Sheppard P., Jelinek L., Whitmore T., Blumberg H., Lehner J., O'Hara P.
Submitted (OCT-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Kidney.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Liver.
[6]"The endothelial-cell-derived secreted factor Egfl7 regulates vascular tube formation."
Parker L.H., Schmidt M., Jin S.-W., Gray A.M., Beis D., Pham T., Frantz G., Palmieri S., Hillan K., Stainier D.Y.R., De Sauvage F.J., Ye W.
Nature 428:754-758(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY239289 mRNA. Translation: AAP69825.1.
AY239290 mRNA. Translation: AAP69826.1.
AY309459 mRNA. Translation: AAP74732.1. Different initiation.
AF184973 mRNA. Translation: AAF01322.1. Different initiation.
AK002601 mRNA. Translation: BAB22222.1. Different initiation.
BC024610 mRNA. Translation: AAH24610.2.
RefSeqNP_001158036.1. NM_001164564.1.
NP_848538.3. NM_178444.4.
NP_942017.2. NM_198724.2.
NP_942018.2. NM_198725.2.
UniGeneMm.268933.

3D structure databases

ProteinModelPortalQ9QXT5.
SMRQ9QXT5. Positions 87-180.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid237257. 4 interactions.
IntActQ9QXT5. 5 interactions.
STRING10090.ENSMUSP00000099970.

PTM databases

PhosphoSiteQ9QXT5.

Proteomic databases

PRIDEQ9QXT5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000100290; ENSMUSP00000097863; ENSMUSG00000026921.
GeneID353156.
KEGGmmu:353156.

Organism-specific databases

CTD51162.
MGIMGI:2449923. Egfl7.

Phylogenomic databases

eggNOGNOG292943.
GeneTreeENSGT00730000110993.
HOGENOMHOG000231494.
HOVERGENHBG051453.
InParanoidQ9QXT5.

Gene expression databases

BgeeQ9QXT5.
CleanExMM_EGFL7.
GenevestigatorQ9QXT5.

Family and domain databases

InterProIPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd_dom.
IPR013032. EGF-like_CS.
IPR018097. EGF_Ca-bd_CS.
IPR011489. EMI_domain.
[Graphical view]
PfamPF07645. EGF_CA. 1 hit.
PF07546. EMI. 1 hit.
[Graphical view]
SMARTSM00181. EGF. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS01187. EGF_CA. 1 hit.
PS51041. EMI. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio400295.
PROQ9QXT5.
SOURCESearch...

Entry information

Entry nameEGFL7_MOUSE
AccessionPrimary (citable) accession number: Q9QXT5
Secondary accession number(s): Q6XD35, Q9DCP5
Entry history
Integrated into UniProtKB/Swiss-Prot: October 25, 2004
Last sequence update: January 4, 2005
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot