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Protein

Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide

Gene

Dapp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.

GO - Molecular functioni

Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide
Short name:
mDAPP1
Alternative name(s):
B lymphocyte adapter protein Bam32
B-cell adapter molecule of 32 kDa
Gene namesi
Name:Dapp1
Synonyms:Bam32
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1347063. Dapp1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 280280Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositidePRO_0000079786Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei139 – 1391PhosphotyrosineCombined sources
Modified residuei141 – 1411PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated on tyrosine residues.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9QXT1.
MaxQBiQ9QXT1.
PaxDbiQ9QXT1.
PRIDEiQ9QXT1.

PTM databases

iPTMnetiQ9QXT1.
PhosphoSiteiQ9QXT1.

Expressioni

Inductioni

Upon B-cell activation.By similarity

Gene expression databases

BgeeiQ9QXT1.
ExpressionAtlasiQ9QXT1. baseline and differential.
GenevisibleiQ9QXT1. MM.

Interactioni

Subunit structurei

Interacts with PtdIns(3,4,5)P3 and PLCG2.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029806.

Structurei

3D structure databases

ProteinModelPortaliQ9QXT1.
SMRiQ9QXT1. Positions 35-109, 157-262.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 12995SH2PROSITE-ProRule annotationAdd
BLAST
Domaini164 – 25996PHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiENOG410IF6C. Eukaryota.
ENOG410XSW5. LUCA.
GeneTreeiENSGT00820000127100.
HOGENOMiHOG000112147.
HOVERGENiHBG051297.
InParanoidiQ9QXT1.
KOiK12229.
OMAiKVNCFCL.
OrthoDBiEOG75TMCS.
PhylomeDBiQ9QXT1.
TreeFamiTF105418.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000980. SH2.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QXT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRAELLGGN MSTQDPSELW GRADGGTDLL QDLGWYHGNL TRHAAEALLL
60 70 80 90 100
SNGRDGSYLL RDSNEQTGLY SLSVRAKDSV KHFHVEYTGY SFKFGFNEYS
110 120 130 140 150
SLKDFVKHFA NQPLIGSETG TLMVLKHPYP REVEEPCIYE SVRVHTAMQT
160 170 180 190 200
GRTENDLVPT APSLGTKEGY LTKQGGLVKT WKTRWFTLQR NELKYFKDQM
210 220 230 240 250
SPEPIRILDL TECSAVQFDY SQERVNCFCL VFPFRTFYLC AKTGVEADEW
260 270 280
IKILRWKLSK IRKQLDQGED TVRSRSFIFK
Length:280
Mass (Da):32,167
Last modified:May 1, 2000 - v1
Checksum:iD15CF545A710F7F0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti90 – 901Y → C in AAD49698 (PubMed:10432293).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163255 mRNA. Translation: AAD49698.1.
AF186023 mRNA. Translation: AAF14579.1.
BC014759 mRNA. Translation: AAH14759.1.
CCDSiCCDS17864.1.
RefSeqiNP_036062.1. NM_011932.3.
UniGeneiMm.254835.
Mm.470427.

Genome annotation databases

EnsembliENSMUST00000029806; ENSMUSP00000029806; ENSMUSG00000028159.
GeneIDi26377.
KEGGimmu:26377.
UCSCiuc008rmt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF163255 mRNA. Translation: AAD49698.1.
AF186023 mRNA. Translation: AAF14579.1.
BC014759 mRNA. Translation: AAH14759.1.
CCDSiCCDS17864.1.
RefSeqiNP_036062.1. NM_011932.3.
UniGeneiMm.254835.
Mm.470427.

3D structure databases

ProteinModelPortaliQ9QXT1.
SMRiQ9QXT1. Positions 35-109, 157-262.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000029806.

PTM databases

iPTMnetiQ9QXT1.
PhosphoSiteiQ9QXT1.

Proteomic databases

EPDiQ9QXT1.
MaxQBiQ9QXT1.
PaxDbiQ9QXT1.
PRIDEiQ9QXT1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029806; ENSMUSP00000029806; ENSMUSG00000028159.
GeneIDi26377.
KEGGimmu:26377.
UCSCiuc008rmt.1. mouse.

Organism-specific databases

CTDi27071.
MGIiMGI:1347063. Dapp1.

Phylogenomic databases

eggNOGiENOG410IF6C. Eukaryota.
ENOG410XSW5. LUCA.
GeneTreeiENSGT00820000127100.
HOGENOMiHOG000112147.
HOVERGENiHBG051297.
InParanoidiQ9QXT1.
KOiK12229.
OMAiKVNCFCL.
OrthoDBiEOG75TMCS.
PhylomeDBiQ9QXT1.
TreeFamiTF105418.

Enzyme and pathway databases

ReactomeiR-MMU-983695. Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.

Miscellaneous databases

PROiQ9QXT1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9QXT1.
ExpressionAtlasiQ9QXT1. baseline and differential.
GenevisibleiQ9QXT1. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000980. SH2.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00017. SH2. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
SMARTiSM00233. PH. 1 hit.
SM00252. SH2. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "DAPP1: a dual adaptor for phosphotyrosine and 3-phosphoinositides."
    Dowler S., Currie R.A., Downes C.P., Alessi D.R.
    Biochem. J. 342:7-12(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "A novel B lymphocyte-associated adaptor protein, Bam32, regulates antigen receptor signaling downstream of phosphatidylinositol 3-kinase."
    Marshall A.J., Niiro H., Lerner C.G., Yun T.J., Thomas S., Disteche C.M., Clark E.A.
    J. Exp. Med. 191:1319-1332(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/cJ.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling."
    Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R.
    J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-139 AND SER-141, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Mast cell.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-139, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDAPP1_MOUSE
AccessioniPrimary (citable) accession number: Q9QXT1
Secondary accession number(s): Q9R178
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: May 1, 2000
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.