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Protein

Protein Wnt-5a

Gene

Wnt5a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells (By similarity). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cartilage development Source: UniProtKB-KW
  • cell differentiation Source: RGD
  • cell fate commitment Source: GO_Central
  • estrous cycle Source: RGD
  • lung alveolus development Source: RGD
  • neuron differentiation Source: RGD
  • positive regulation of cell-cell adhesion Source: RGD
  • positive regulation of neuron projection arborization Source: ARUK-UCL
  • prostate gland development Source: RGD
  • response to estradiol Source: RGD
  • response to glucocorticoid Source: RGD
  • response to hyperoxia Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
  • response to testosterone Source: RGD
  • Wnt signaling pathway, planar cell polarity pathway Source: MGI

Keywordsi

Molecular functionDevelopmental protein
Biological processChondrogenesis, Differentiation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-5a
Gene namesi
Name:Wnt5a
Synonyms:Wnt-5a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69250 Wnt5a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 37Sequence analysisAdd BLAST37
PropeptideiPRO_000035279838 – 61By similarityAdd BLAST24
ChainiPRO_000004142962 – 380Protein Wnt-5aAdd BLAST319

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi104 ↔ 115By similarity
Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi120N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi154 ↔ 162By similarity
Disulfide bondi164 ↔ 182By similarity
Disulfide bondi238 ↔ 252By similarity
Disulfide bondi240 ↔ 247By similarity
Lipidationi244O-palmitoleoyl serine; by PORCNBy similarity1
Glycosylationi312N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi325 ↔ 340By similarity
Glycosylationi326N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi355 ↔ 370By similarity
Disulfide bondi357 ↔ 367By similarity
Disulfide bondi362 ↔ 363By similarity

Post-translational modificationi

Glycosylation is necessary for secretion but not for activity.By similarity
Palmitoleoylation is required for efficient binding to frizzled receptors. Depalmitoleoylation leads to Wnt signaling pathway inhibition.By similarity
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiQ9QXQ7
PRIDEiQ9QXQ7

Interactioni

Subunit structurei

Forms a soluble 1:1 complex with AFM; this prevents oligomerization and is required for prolonged biological activity. The complex with AFM may represent the physiological form in body fluids (By similarity). Homooligomer; disulfide-linked, leading to inactivation. Interacts with PORCN. Interacts with WLS (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021164

Structurei

3D structure databases

SMRiQ9QXQ7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913 Eukaryota
ENOG410XQZ1 LUCA
HOGENOMiHOG000039529
HOVERGENiHBG001595
InParanoidiQ9QXQ7
PhylomeDBiQ9QXQ7

Family and domain databases

InterProiView protein in InterPro
IPR005817 Wnt
IPR026538 Wnt5a
IPR018161 Wnt_CS
PANTHERiPTHR12027 PTHR12027, 1 hit
PTHR12027:SF33 PTHR12027:SF33, 1 hit
PfamiView protein in Pfam
PF00110 wnt, 1 hit
PRINTSiPR01349 WNTPROTEIN
SMARTiView protein in SMART
SM00097 WNT1, 1 hit
PROSITEiView protein in PROSITE
PS00246 WNT1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QXQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPIGILSP GVALGTAGGA MSSKFFLMAL ATFFSFAQVV IEANSWWSLG
60 70 80 90 100
MNNPVQMSEV HIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG
110 120 130 140 150
IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM
160 170 180 190 200
SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR
210 220 230 240 250
ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK
260 270 280 290 300
TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV
310 320 330 340 350
YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV
360 370 380
QTERCHCKFH WCCYVKCKKC TEIVDQFVCK
Length:380
Mass (Da):42,283
Last modified:November 4, 2008 - v2
Checksum:i9716401010072224
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti42E → K in AAF15588 (Ref. 2) Curated1
Sequence conflicti50G → S in AAF15588 (Ref. 2) Curated1
Sequence conflicti61H → Y in AAF15588 (Ref. 2) Curated1
Sequence conflicti168Missing in AAF15588 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY819646 mRNA Translation: AAV69750.1
AF188333 mRNA Translation: AAF15588.1
UniGeneiRn.48749

Genome annotation databases

UCSCiRGD:69250 rat

Similar proteinsi

Entry informationi

Entry nameiWNT5A_RAT
AccessioniPrimary (citable) accession number: Q9QXQ7
Secondary accession number(s): Q5PY99
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 4, 2008
Last modified: May 23, 2018
This is version 116 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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