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Protein

Protein Wnt-5a

Gene

Wnt5a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression. Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor. Mediates motility of melanoma cells. Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • cartilage development Source: UniProtKB-KW
  • cell differentiation Source: RGD
  • cell fate commitment Source: GO_Central
  • estrous cycle Source: RGD
  • lung alveolus development Source: RGD
  • neuron differentiation Source: RGD
  • positive regulation of cell-cell adhesion Source: RGD
  • prostate gland development Source: RGD
  • response to estradiol Source: RGD
  • response to glucocorticoid Source: RGD
  • response to hyperoxia Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
  • response to testosterone Source: RGD
  • Wnt signaling pathway, planar cell polarity pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Chondrogenesis, Differentiation, Wnt signaling pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Protein Wnt-5a
Gene namesi
Name:Wnt5a
Synonyms:Wnt-5a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69250. Wnt5a.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • extracellular space Source: RGD
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3737Sequence analysisAdd
BLAST
Propeptidei38 – 6124By similarityPRO_0000352798Add
BLAST
Chaini62 – 380319Protein Wnt-5aPRO_0000041429Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi104 ↔ 115By similarity
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence analysis
Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence analysis
Disulfide bondi154 ↔ 162By similarity
Disulfide bondi164 ↔ 182By similarity
Disulfide bondi238 ↔ 252By similarity
Disulfide bondi240 ↔ 247By similarity
Lipidationi244 – 2441O-palmitoleyl serine; by PORCNBy similarity
Glycosylationi312 – 3121N-linked (GlcNAc...)Sequence analysis
Disulfide bondi325 ↔ 340By similarity
Glycosylationi326 – 3261N-linked (GlcNAc...)Sequence analysis
Disulfide bondi355 ↔ 370By similarity
Disulfide bondi357 ↔ 367By similarity
Disulfide bondi362 ↔ 363By similarity

Post-translational modificationi

Glycosylation is necessary for secretion but not for activity.By similarity
Palmitoleylation is required for efficient binding to frizzled receptors. Depalmitoleylation leads to Wnt signaling pathway inhibition.By similarity
Proteolytic processing by TIKI1 and TIKI2 promotes oxidation and formation of large disulfide-bond oligomers, leading to inactivation of WNT5A.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein

Proteomic databases

PaxDbiQ9QXQ7.

Interactioni

Subunit structurei

Homooligomer; disulfide-linked, leading to inactivation. Interacts with PORCN. Interacts with WLS (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021164.

Family & Domainsi

Sequence similaritiesi

Belongs to the Wnt family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3913. Eukaryota.
ENOG410XQZ1. LUCA.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiQ9QXQ7.
PhylomeDBiQ9QXQ7.

Family and domain databases

InterProiIPR005817. Wnt.
IPR026538. Wnt5a.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF33. PTHR12027:SF33. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9QXQ7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPIGILSP GVALGTAGGA MSSKFFLMAL ATFFSFAQVV IEANSWWSLG
60 70 80 90 100
MNNPVQMSEV HIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG
110 120 130 140 150
IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM
160 170 180 190 200
SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR
210 220 230 240 250
ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK
260 270 280 290 300
TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV
310 320 330 340 350
YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV
360 370 380
QTERCHCKFH WCCYVKCKKC TEIVDQFVCK
Length:380
Mass (Da):42,283
Last modified:November 4, 2008 - v2
Checksum:i9716401010072224
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti42 – 421E → K in AAF15588 (Ref. 2) Curated
Sequence conflicti50 – 501G → S in AAF15588 (Ref. 2) Curated
Sequence conflicti61 – 611H → Y in AAF15588 (Ref. 2) Curated
Sequence conflicti168 – 1681Missing in AAF15588 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY819646 mRNA. Translation: AAV69750.1.
AF188333 mRNA. Translation: AAF15588.1.
UniGeneiRn.48749.

Genome annotation databases

UCSCiRGD:69250. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY819646 mRNA. Translation: AAV69750.1.
AF188333 mRNA. Translation: AAF15588.1.
UniGeneiRn.48749.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000021164.

Proteomic databases

PaxDbiQ9QXQ7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:69250. rat.

Organism-specific databases

RGDi69250. Wnt5a.

Phylogenomic databases

eggNOGiKOG3913. Eukaryota.
ENOG410XQZ1. LUCA.
HOGENOMiHOG000039529.
HOVERGENiHBG001595.
InParanoidiQ9QXQ7.
PhylomeDBiQ9QXQ7.

Miscellaneous databases

PROiQ9QXQ7.

Family and domain databases

InterProiIPR005817. Wnt.
IPR026538. Wnt5a.
IPR018161. Wnt_CS.
[Graphical view]
PANTHERiPTHR12027. PTHR12027. 1 hit.
PTHR12027:SF33. PTHR12027:SF33. 1 hit.
PfamiPF00110. wnt. 1 hit.
[Graphical view]
PRINTSiPR01349. WNTPROTEIN.
SMARTiSM00097. WNT1. 1 hit.
[Graphical view]
PROSITEiPS00246. WNT1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWNT5A_RAT
AccessioniPrimary (citable) accession number: Q9QXQ7
Secondary accession number(s): Q5PY99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 4, 2008
Last modified: July 6, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.