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Protein

Inositol oxygenase

Gene

Miox

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Myo-inositol + O2 = D-glucuronate + H2O.

Cofactori

Fe cation2 PublicationsNote: Binds 2 iron ions per subunit.2 Publications

Pathwayi: myo-inositol degradation into D-glucuronate

This protein is involved in step 1 of the subpathway that synthesizes D-glucuronate from myo-inositol.
Proteins known to be involved in this subpathway in this organism are:
  1. Inositol oxygenase (Miox)
This subpathway is part of the pathway myo-inositol degradation into D-glucuronate, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glucuronate from myo-inositol, the pathway myo-inositol degradation into D-glucuronate and in Polyol metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29Substrate1
Metal bindingi98Iron 12 Publications1
Metal bindingi123Iron 12 Publications1
Metal bindingi124Iron 12 Publications1
Metal bindingi124Iron 22 Publications1
Binding sitei127Substrate1
Metal bindingi194Iron 22 Publications1
Metal bindingi220Iron 22 Publications1
Metal bindingi253Iron 12 Publications1

GO - Molecular functioni

  • aldo-keto reductase (NADP) activity Source: UniProtKB
  • ferric iron binding Source: UniProtKB
  • inositol oxygenase activity Source: UniProtKB
  • oxidoreductase activity Source: MGI
  • oxidoreductase activity, acting on NAD(P)H Source: UniProtKB
  • oxidoreductase activity, acting on single donors with incorporation of molecular oxygen Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.99.1. 3474.
SABIO-RKQ9QXN5.
UniPathwayiUPA00111; UER00527.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol oxygenase (EC:1.13.99.1)
Alternative name(s):
Aldehyde reductase-like 6
Myo-inositol oxygenase
Short name:
MI oxygenase
Renal-specific oxidoreductase
Gene namesi
Name:Miox
Synonyms:Aldrl6, Rsor
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1891725. Miox.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: MGI
  • inclusion body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000791491 – 285Inositol oxygenaseAdd BLAST285

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei33PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9QXN5.
PaxDbiQ9QXN5.
PeptideAtlasiQ9QXN5.
PRIDEiQ9QXN5.

2D gel databases

REPRODUCTION-2DPAGEQ9QXN5.

PTM databases

iPTMnetiQ9QXN5.
PhosphoSitePlusiQ9QXN5.

Expressioni

Tissue specificityi

Kidney specific. Renal proximal tubules.1 Publication

Gene expression databases

BgeeiENSMUSG00000022613.
CleanExiMM_MIOX.
ExpressionAtlasiQ9QXN5. baseline and differential.
GenevisibleiQ9QXN5. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9QXN5. 1 interactor.
MINTiMINT-4102022.
STRINGi10090.ENSMUSP00000023282.

Structurei

Secondary structure

1285
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi31 – 33Combined sources3
Helixi37 – 50Combined sources14
Helixi53 – 63Combined sources11
Helixi73 – 79Combined sources7
Helixi80 – 82Combined sources3
Helixi95 – 109Combined sources15
Helixi114 – 122Combined sources9
Helixi125 – 132Combined sources8
Helixi136 – 138Combined sources3
Beta strandi145 – 148Combined sources4
Turni155 – 159Combined sources5
Helixi165 – 168Combined sources4
Turni170 – 172Combined sources3
Beta strandi173 – 176Combined sources4
Helixi185 – 187Combined sources3
Helixi194 – 204Combined sources11
Helixi211 – 219Combined sources9
Helixi223 – 226Combined sources4
Turni232 – 234Combined sources3
Helixi237 – 255Combined sources19
Helixi264 – 278Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HUOX-ray2.00A1-285[»]
3BXDX-ray2.00A1-285[»]
ProteinModelPortaliQ9QXN5.
SMRiQ9QXN5.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9QXN5.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 88Substrate binding4
Regioni141 – 142Substrate binding2
Regioni220 – 221Substrate binding2

Sequence similaritiesi

Belongs to the myo-inositol oxygenase family.Curated

Phylogenomic databases

eggNOGiKOG1573. Eukaryota.
ENOG410XQ4J. LUCA.
GeneTreeiENSGT00390000016211.
HOGENOMiHOG000163182.
HOVERGENiHBG039556.
InParanoidiQ9QXN5.
KOiK00469.
OMAiNFYREQH.
OrthoDBiEOG091G0FIX.
PhylomeDBiQ9QXN5.
TreeFamiTF300089.

Family and domain databases

InterProiIPR018170. Aldo/ket_reductase_CS.
IPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. MIOX. 1 hit.
[Graphical view]
PROSITEiPS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QXN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVDVGPDPS LVYRPDVDPE MAKSKDSFRN YTSGPLLDRV FTTYKLMHTH
60 70 80 90 100
QTVDFVSRKR IQYGSFSYKK MTIMEAVGML DDLVDESDPD VDFPNSFHAF
110 120 130 140 150
QTAEGIRKAH PDKDWFHLVG LLHDLGKIMA LWGEPQWAVV GDTFPVGCRP
160 170 180 190 200
QASVVFCDST FQDNPDLQDP RYSTELGMYQ PHCGLENVLM SWGHDEYLYQ
210 220 230 240 250
MMKFNKFSLP SEAFYMIRFH SFYPWHTGGD YRQLCSQQDL DMLPWVQEFN
260 270 280
KFDLYTKCPD LPDVESLRPY YQGLIDKYCP GTLSW
Length:285
Mass (Da):33,164
Last modified:November 8, 2005 - v2
Checksum:iC6877BFF2B60D1A7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti65S → G in AAF25202 (PubMed:10944187).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197127 mRNA. Translation: AAF25202.1.
AY738257 mRNA. Translation: AAV65815.1.
BC013543 mRNA. Translation: AAH13543.1.
CCDSiCCDS27745.1.
RefSeqiNP_064361.2. NM_019977.2.
UniGeneiMm.158200.

Genome annotation databases

EnsembliENSMUST00000023282; ENSMUSP00000023282; ENSMUSG00000022613.
GeneIDi56727.
KEGGimmu:56727.
UCSCiuc007xgc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197127 mRNA. Translation: AAF25202.1.
AY738257 mRNA. Translation: AAV65815.1.
BC013543 mRNA. Translation: AAH13543.1.
CCDSiCCDS27745.1.
RefSeqiNP_064361.2. NM_019977.2.
UniGeneiMm.158200.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HUOX-ray2.00A1-285[»]
3BXDX-ray2.00A1-285[»]
ProteinModelPortaliQ9QXN5.
SMRiQ9QXN5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9QXN5. 1 interactor.
MINTiMINT-4102022.
STRINGi10090.ENSMUSP00000023282.

PTM databases

iPTMnetiQ9QXN5.
PhosphoSitePlusiQ9QXN5.

2D gel databases

REPRODUCTION-2DPAGEQ9QXN5.

Proteomic databases

MaxQBiQ9QXN5.
PaxDbiQ9QXN5.
PeptideAtlasiQ9QXN5.
PRIDEiQ9QXN5.

Protocols and materials databases

DNASUi56727.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023282; ENSMUSP00000023282; ENSMUSG00000022613.
GeneIDi56727.
KEGGimmu:56727.
UCSCiuc007xgc.1. mouse.

Organism-specific databases

CTDi55586.
MGIiMGI:1891725. Miox.

Phylogenomic databases

eggNOGiKOG1573. Eukaryota.
ENOG410XQ4J. LUCA.
GeneTreeiENSGT00390000016211.
HOGENOMiHOG000163182.
HOVERGENiHBG039556.
InParanoidiQ9QXN5.
KOiK00469.
OMAiNFYREQH.
OrthoDBiEOG091G0FIX.
PhylomeDBiQ9QXN5.
TreeFamiTF300089.

Enzyme and pathway databases

UniPathwayiUPA00111; UER00527.
BRENDAi1.13.99.1. 3474.
SABIO-RKQ9QXN5.

Miscellaneous databases

EvolutionaryTraceiQ9QXN5.
PROiQ9QXN5.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022613.
CleanExiMM_MIOX.
ExpressionAtlasiQ9QXN5. baseline and differential.
GenevisibleiQ9QXN5. MM.

Family and domain databases

InterProiIPR018170. Aldo/ket_reductase_CS.
IPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. MIOX. 1 hit.
[Graphical view]
PROSITEiPS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMIOX_MOUSE
AccessioniPrimary (citable) accession number: Q9QXN5
Secondary accession number(s): Q5S8D0, Q91WQ8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: November 8, 2005
Last modified: November 2, 2016
This is version 129 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.