Inositol oxygenase - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot Q9QXN5 (MIOX_MOUSE)

Last modified June 16, 2009. Version 70. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Inositol oxygenase
    EC=1.13.99.1
Alternative name(s):
    Myo-inositol oxygenase
      Short name=MI oxygenase
    Aldehyde reductase-like 6
    Renal-specific oxidoreductase

Gene names

Name:	Miox
Synonyms:	Aldrl6, Rsor

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	Myo-inositol + O₂ = D-glucuronate + H₂O.
Cofactor	Binds 2 iron ions per subunit.
Pathway	Polyol metabolism; myo-inositol degradation into D-glucuronic acid; D-glucuronic acid from myo-inositol: step 1/1.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Kidney specific. Renal proximal tubules. Ref.1
Sequence similarities	Belongs to the myo-inositol oxygenase family.

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Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Ref.1 Inferred from direct assay. Source: UniProtKB inclusion body Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	aldo-keto reductase activity Ref.1 Inferred from direct assay. Source: UniProtKB inositol oxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on NADH or NADPH Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 285

285

Inositol oxygenase

PRO_0000079149

Sites

Metal binding

Iron 1

Metal binding

123

Iron 1

Metal binding

124

Iron 1

Metal binding

124

Iron 2

Metal binding

194

Iron 2

Metal binding

220

Iron 2

Metal binding

253

Iron 1

Experimental info

Sequence conflict

S → G in AAF25202. Ref.1

Secondary structure

285

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9QXN5-1 [UniParc].

Last modified November 8, 2005. Version 2.
Checksum: C6877BFF2B60D1A7
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FASTA

285

33,164

        10         20         30         40         50         60 
MKVDVGPDPS LVYRPDVDPE MAKSKDSFRN YTSGPLLDRV FTTYKLMHTH QTVDFVSRKR 

        70         80         90        100        110        120 
IQYGSFSYKK MTIMEAVGML DDLVDESDPD VDFPNSFHAF QTAEGIRKAH PDKDWFHLVG 

       130        140        150        160        170        180 
LLHDLGKIMA LWGEPQWAVV GDTFPVGCRP QASVVFCDST FQDNPDLQDP RYSTELGMYQ 

       190        200        210        220        230        240 
PHCGLENVLM SWGHDEYLYQ MMKFNKFSLP SEAFYMIRFH SFYPWHTGGD YRQLCSQQDL 

       250        260        270        280 
DMLPWVQEFN KFDLYTKCPD LPDVESLRPY YQGLIDKYCP GTLSW

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a renal-specific oxido-reductase in newborn diabetic mice." Yang Q., Dixit B., Wada J., Tian Y., Wallner E.I., Srivastva S.K., Kanwar Y.S. Proc. Natl. Acad. Sci. U.S.A. 97:9896-9901(2000) [PubMed: 10944187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Kidney.
[2]	"Molecular cloning, expression, and characterization of myo-inositol oxygenase from mouse, rat, and human kidney." Arner R.J., Prabhu K.S., Reddy C.C. Biochem. Biophys. Res. Commun. 324:1386-1392(2004) [PubMed: 15504367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Kidney.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[4]	"Crystal structure of a substrate complex of myo-inositol oxygenase, a di-iron oxygenase with a key role in inositol metabolism." Brown P.M., Caradoc-Davies T.T., Dickson J.M., Cooper G.J., Loomes K.M., Baker E.N. Proc. Natl. Acad. Sci. U.S.A. 103:15032-15037(2006) [PubMed: 17012379] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+	Additional computationally mapped references.

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Cross-references

Sequence databases

AF197127 mRNA. Translation: AAF25202.1.
AY738257 mRNA. Translation: AAV65815.1.
BC013543 mRNA. Translation: AAH13543.1.

IPI

IPI00128313.

RefSeq

NP_064361.2.

UniGene

Mm.158200

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HUO	X-ray	2.00	A	1-285	[»]
3BXD	X-ray	2.00	A	1-285	[»]

ModBase

Search...

2-D gel databases

REPRODUCTION-2DPAGE

Q9QXN5.

Proteomic databases

PRIDE

Q9QXN5.

Genome annotation databases

Ensembl

ENSMUSG00000022613. Mus musculus. [Contig view]

GeneID

56727.

KEGG

mmu:56727.

NMPDR

fig|10090.3.peg.30445.

Organism-specific databases

MGI

MGI:1891725. Miox.

Phylogenomic databases

HOGENOM

Q9QXN5.

HOVERGEN

Q9QXN5.

OMA

Q9QXN5. YQVMKFN.

Enzyme and pathway databases

BRENDA

1.13.99.1. 244.

Gene expression databases

ArrayExpress

Q9QXN5.

Bgee

Q9QXN5.

CleanEx

MM_MIOX.

GermOnline

ENSMUSG00000022613. Mus musculus.

Family and domain databases

InterPro

IPR018170. Aldo/ket_reductase_CS.
IPR007828. DUF706.
[Graphical view]

PANTHER

PTHR12588. DUF706. 1 hit.

Pfam

PF05153. DUF706. 1 hit.
[Graphical view]

PROSITE

PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

313210.

SOURCE

Search...

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Entry information

Entry name

MIOX_MOUSE

Accession

Primary (citable) accession number: Q9QXN5
Secondary accession number(s): Q5S8D0, Q91WQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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