Reviewed, UniProtKB/Swiss-Prot Q9QXN4 (MIOX_RAT)

Names and origin

Protein names

Recommended name:
    Inositol oxygenase
    EC=1.13.99.1
Alternative name(s):
    Myo-inositol oxygenase
      Short name=MI oxygenase
    Aldehyde reductase-like 6
    Renal-specific oxidoreductase
    Kidney-specific protein 32

Gene names

Name:	Miox
Synonyms:	Aldrl6, Ksp32, Rsor

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Myo-inositol + O2 = D-glucuronate + H2O.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Polyol metabolism; myo-inositol degradation into D-glucuronic acid; D-glucuronic acid from myo-inositol: step 1/1.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Kidney specific.
Sequence similarities	Belongs to the myo-inositol oxygenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	inositol catabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB inclusion body Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	NADP or NADPH binding Ref.1 Inferred from direct assay. Source: RGD aldo-keto reductase activity Inferred from sequence or structural similarity. Source: UniProtKB inositol oxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Inositol oxygenase		PRO_0000079152
Sites
	Metal binding	98	1	Iron 1 By similarity
	Metal binding	123	1	Iron 1 By similarity
	Metal binding	124	1	Iron 1 By similarity
	Metal binding	124	1	Iron 2 By similarity
	Metal binding	194	1	Iron 2 By similarity
	Metal binding	220	1	Iron 2 By similarity
	Metal binding	253	1	Iron 1 By similarity
Experimental info
	Sequence conflict	4 – 5	2	DL → TV in AAK00767. Ref.2
	Sequence conflict	21	1	M → V in AAK00767. Ref.2
	Sequence conflict	24 – 26	3	SKG → DKA in AAK00767. Ref.2
	Sequence conflict	57	1	M → R Ref.2
	Sequence conflict	57	1	M → V in AAV65817. Ref.3
	Sequence conflict	58 – 61	4	RKRI → SKHA Ref.2
	Sequence conflict	282	1	I → T Ref.1
	Sequence conflict	282	1	I → T Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9QXN4-1 [UniParc]. Last modified November 8, 2005. Version 2. Checksum: F34A26521979AE89	FASTA	285	33,185
	10 20 30 40 50 60 MKVDLGPDPS LVYRPDVDPE MAKSKGSFRN YTSGPLLDRV FTTYKLMHTH QTVDFVMRKR 70 80 90 100 110 120 IQFGSFSYKK MTVMEAVDML DDLVDESDPD VDFPNSFHAF QTAEGIRKAH PDKDWFHLVG 130 140 150 160 170 180 LLHDLGKILA LWGEPQWAVV GDTFPVGCRP QASVVFCDST FQDNPDLQDP RYSTELGMYQ 190 200 210 220 230 240 PHCGLENVLM SWGHDEYLYQ MMKFNKFSLP SEAFYMVRFH SFYPWHTGGD YRQLCSQQDL 250 260 270 280 DMLPWVQEFN KFDLYTKCPD LPEVKSLRPY YQGLIDKYCP GILSW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a renal-specific oxido-reductase in newborn diabetic mice." Yang Q., Dixit B., Wada J., Tian Y., Wallner E.I., Srivastva S.K., Kanwar Y.S. Proc. Natl. Acad. Sci. U.S.A. 97:9896-9901(2000) [PubMed: 10944187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[2]	"Identification of a novel kidney specific gene, KSP32, that is down regulated in acute ischemic renal failure." Hu E., Chen Z., Fredrickson T., Gellai M., Jugus M., Contino L., Spurr N., Sims M., Halsey W., Van Horn S., Mao J., Sathe G., Brooks D. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Molecular cloning, expression, and characterization of myo-inositol oxygenase from mouse, rat, and human kidney." Arner R.J., Prabhu K.S., Reddy C.C. Biochem. Biophys. Res. Commun. 324:1386-1392(2004) [PubMed: 15504367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Long Evans. Tissue: Kidney.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF197128 mRNA. Translation: AAF25203.1. AF230096 mRNA. Translation: AAK00767.1. AY738259 mRNA. Translation: AAV65817.1. BC078840 mRNA. Translation: AAH78840.1.
IPI	IPI00213380.
RefSeq	NP_665714.2.
UniGene	Rn.19959
3D structure databases
SMR	Q9QXN4. Positions 28-285.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000008694. Rattus norvegicus. [Contig view]
GeneID	252899.
KEGG	rno:252899.
Organism-specific databases
RGD	628739. Miox.
Phylogenomic databases
HOVERGEN	Q9QXN4.
OMA	Q9QXN4. YQVMKFN.
Enzyme and pathway databases
BRENDA	1.13.99.1. 248.
Gene expression databases
ArrayExpress	Q9QXN4.
GermOnline	ENSRNOG00000008694. Rattus norvegicus.
Family and domain databases
InterPro	IPR018170. Aldo/ket_reductase_CS. IPR007828. DUF706. [Graphical view]
PANTHER	PTHR12588. DUF706. 1 hit.
Pfam	PF05153. DUF706. 1 hit. [Graphical view]
PROSITE	PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	624027.

Entry information

Entry name

MIOX_RAT

Accession

Primary (citable) accession number: Q9QXN4
Secondary accession number(s): Q5S8C8, Q68G06, Q99PN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 12, 2003
Last sequence update:	November 8, 2005
Last modified:	June 16, 2009
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)