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Protein

Inositol oxygenase

Gene

Miox

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Myo-inositol + O2 = D-glucuronate + H2O.

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei29 – 291SubstrateBy similarity
Metal bindingi98 – 981Iron 1By similarity
Metal bindingi123 – 1231Iron 1By similarity
Metal bindingi124 – 1241Iron 1By similarity
Metal bindingi124 – 1241Iron 2By similarity
Binding sitei127 – 1271SubstrateBy similarity
Metal bindingi194 – 1941Iron 2By similarity
Metal bindingi220 – 2201Iron 2By similarity
Metal bindingi253 – 2531Iron 1By similarity

GO - Molecular functioni

  1. aldo-keto reductase (NADP) activity Source: UniProtKB
  2. ferric iron binding Source: UniProtKB
  3. inositol oxygenase activity Source: UniProtKB
  4. NADP binding Source: RGD
  5. oxidoreductase activity, acting on NAD(P)H Source: Ensembl
  6. oxidoreductase activity, acting on single donors with incorporation of molecular oxygen Source: UniProtKB

GO - Biological processi

  1. inositol catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.99.1. 5301.
SABIO-RKQ9QXN4.
UniPathwayiUPA00111; UER00527.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol oxygenase (EC:1.13.99.1)
Alternative name(s):
Aldehyde reductase-like 6
Kidney-specific protein 32
Myo-inositol oxygenase
Short name:
MI oxygenase
Renal-specific oxidoreductase
Gene namesi
Name:Miox
Synonyms:Aldrl6, Ksp32, Rsor
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi628739. Miox.

Subcellular locationi

  1. Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. extracellular vesicular exosome Source: Ensembl
  3. inclusion body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285Inositol oxygenasePRO_0000079152Add
BLAST

Proteomic databases

PRIDEiQ9QXN4.

PTM databases

PhosphoSiteiQ9QXN4.

Expressioni

Tissue specificityi

Kidney specific.

Gene expression databases

GenevestigatoriQ9QXN4.

Structurei

3D structure databases

ProteinModelPortaliQ9QXN4.
SMRiQ9QXN4. Positions 28-285.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni85 – 873Substrate bindingBy similarity
Regioni141 – 1422Substrate bindingBy similarity
Regioni220 – 2212Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the myo-inositol oxygenase family.Curated

Phylogenomic databases

eggNOGiNOG135479.
GeneTreeiENSGT00390000016211.
HOGENOMiHOG000163182.
HOVERGENiHBG039556.
InParanoidiQ9QXN4.
KOiK00469.
OMAiPEAFYMI.
OrthoDBiEOG7DZ8KD.
PhylomeDBiQ9QXN4.
TreeFamiTF300089.

Family and domain databases

InterProiIPR018170. Aldo/ket_reductase_CS.
IPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. DUF706. 1 hit.
[Graphical view]
PROSITEiPS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9QXN4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVDLGPDPS LVYRPDVDPE MAKSKGSFRN YTSGPLLDRV FTTYKLMHTH
60 70 80 90 100
QTVDFVMRKR IQFGSFSYKK MTVMEAVDML DDLVDESDPD VDFPNSFHAF
110 120 130 140 150
QTAEGIRKAH PDKDWFHLVG LLHDLGKILA LWGEPQWAVV GDTFPVGCRP
160 170 180 190 200
QASVVFCDST FQDNPDLQDP RYSTELGMYQ PHCGLENVLM SWGHDEYLYQ
210 220 230 240 250
MMKFNKFSLP SEAFYMVRFH SFYPWHTGGD YRQLCSQQDL DMLPWVQEFN
260 270 280
KFDLYTKCPD LPEVKSLRPY YQGLIDKYCP GILSW
Length:285
Mass (Da):33,185
Last modified:November 8, 2005 - v2
Checksum:iF34A26521979AE89
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 52DL → TV in AAK00767 (Ref. 2) Curated
Sequence conflicti21 – 211M → V in AAK00767 (Ref. 2) Curated
Sequence conflicti24 – 263SKG → DKA in AAK00767 (Ref. 2) Curated
Sequence conflicti57 – 571M → R (Ref. 2) Curated
Sequence conflicti57 – 571M → V in AAV65817 (PubMed:15504367).Curated
Sequence conflicti58 – 614RKRI → SKHA (Ref. 2) Curated
Sequence conflicti282 – 2821I → T (PubMed:10944187).Curated
Sequence conflicti282 – 2821I → T (PubMed:15504367).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197128 mRNA. Translation: AAF25203.1.
AF230096 mRNA. Translation: AAK00767.1.
AY738259 mRNA. Translation: AAV65817.1.
BC078840 mRNA. Translation: AAH78840.1.
RefSeqiNP_665714.2. NM_145771.2.
UniGeneiRn.19959.

Genome annotation databases

EnsembliENSRNOT00000011941; ENSRNOP00000011941; ENSRNOG00000008694.
GeneIDi252899.
KEGGirno:252899.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197128 mRNA. Translation: AAF25203.1.
AF230096 mRNA. Translation: AAK00767.1.
AY738259 mRNA. Translation: AAV65817.1.
BC078840 mRNA. Translation: AAH78840.1.
RefSeqiNP_665714.2. NM_145771.2.
UniGeneiRn.19959.

3D structure databases

ProteinModelPortaliQ9QXN4.
SMRiQ9QXN4. Positions 28-285.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9QXN4.

Proteomic databases

PRIDEiQ9QXN4.

Protocols and materials databases

DNASUi252899.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011941; ENSRNOP00000011941; ENSRNOG00000008694.
GeneIDi252899.
KEGGirno:252899.

Organism-specific databases

CTDi55586.
RGDi628739. Miox.

Phylogenomic databases

eggNOGiNOG135479.
GeneTreeiENSGT00390000016211.
HOGENOMiHOG000163182.
HOVERGENiHBG039556.
InParanoidiQ9QXN4.
KOiK00469.
OMAiPEAFYMI.
OrthoDBiEOG7DZ8KD.
PhylomeDBiQ9QXN4.
TreeFamiTF300089.

Enzyme and pathway databases

UniPathwayiUPA00111; UER00527.
BRENDAi1.13.99.1. 5301.
SABIO-RKQ9QXN4.

Miscellaneous databases

NextBioi624027.
PROiQ9QXN4.

Gene expression databases

GenevestigatoriQ9QXN4.

Family and domain databases

InterProiIPR018170. Aldo/ket_reductase_CS.
IPR007828. Inositol_oxygenase.
[Graphical view]
PANTHERiPTHR12588. PTHR12588. 1 hit.
PfamiPF05153. DUF706. 1 hit.
[Graphical view]
PROSITEiPS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a renal-specific oxido-reductase in newborn diabetic mice."
    Yang Q., Dixit B., Wada J., Tian Y., Wallner E.I., Srivastva S.K., Kanwar Y.S.
    Proc. Natl. Acad. Sci. U.S.A. 97:9896-9901(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Identification of a novel kidney specific gene, KSP32, that is down regulated in acute ischemic renal failure."
    Hu E., Chen Z., Fredrickson T., Gellai M., Jugus M., Contino L., Spurr N., Sims M., Halsey W., Van Horn S., Mao J., Sathe G., Brooks D.
    Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Molecular cloning, expression, and characterization of myo-inositol oxygenase from mouse, rat, and human kidney."
    Arner R.J., Prabhu K.S., Reddy C.C.
    Biochem. Biophys. Res. Commun. 324:1386-1392(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Long Evans.
    Tissue: Kidney.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiMIOX_RAT
AccessioniPrimary (citable) accession number: Q9QXN4
Secondary accession number(s): Q5S8C8, Q68G06, Q99PN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 12, 2003
Last sequence update: November 8, 2005
Last modified: January 7, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.