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Protein

Activating signal cointegrator 1

Gene

Trip4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription coactivator which associates with nuclear receptors, transcriptional coactivators including EP300, CREBBP and NCOA1, and basal transcription factors like TBP and TFIIA to facilitate nuclear receptors-mediated transcription. May thereby play an important role in establishing distinct coactivator complexes under different cellular conditions. Plays a role in thyroid hormone receptor and estrogen receptor transactivation (By similarity). Also involved in androgen receptor transactivation (PubMed:12077347). Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. May play a role in the development of neuromuscular junction (By similarity).By similarity2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri167 – 219C4-typeAdd BLAST53

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Activating signal cointegrator 11 Publication
Short name:
ASC-11 Publication
Alternative name(s):
Thyroid receptor-interacting protein 4Curated
Short name:
TR-interacting protein 4Curated
Short name:
TRIP-4Curated
Gene namesi
Name:Trip4Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1928469. Trip4.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasmcytosol By similarity
  • Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity

  • Note: Cytoplasmic under conditions of serum deprivation. Colocalizes with NEK6 in the centrosome.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000656322 – 581Activating signal cointegrator 1Add BLAST580

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei276PhosphoserineBy similarity1
Modified residuei289PhosphotyrosineCombined sources1
Cross-linki324Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1)By similarity
Cross-linki334Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in UFM1)By similarity

Post-translational modificationi

Phosphorylated by NEK6.By similarity
Polyufmylated by the UFM1-conjugating system composed of the enzymes UBA5, UFC1 and UFL1. Deufmylated by the protease UFSP2. Ufmylation of TRIP4 is promoted by ligand-bound nuclear receptors that compete with UFSP2 for interaction with TRIP4. Nuclear receptors-induced ufmylation promotes the recruitment of additional transcriptional coactivators like EP300 and NCOA1 and therefore the assembly of a coactivator complex facilitating nuclear receptor-mediated transcription.By similarity1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9QXN3.
PRIDEiQ9QXN3.

PTM databases

iPTMnetiQ9QXN3.
PhosphoSitePlusiQ9QXN3.

Expressioni

Tissue specificityi

Ubiquitously expressed (PubMed:12390891). Expressed in the spinal cord, brain, paraspinal ganglia, thyroid, and submandibular glands (PubMed:26924529).2 Publications

Developmental stagei

Expressed in 17.5-day-old embryos.1 Publication

Gene expression databases

BgeeiENSMUSG00000032386.
CleanExiMM_TRIP4.
ExpressionAtlasiQ9QXN3. baseline and differential.
GenevisibleiQ9QXN3. MM.

Interactioni

Subunit structurei

Interacts with the thyroid hormone receptor/TR (via the ligand-binding domain); this interaction requires the presence of thyroid hormone (By similarity). Interacts with the androgen receptor/AR; in an androgen, testosterone and dihydrotestosterone-dependent manner (By similarity). Interacts with ESR1 (estrogen ligand-bound); competes with UFSP2 (By similarity). Interacts with UFSP2; competes with ligand-bound ESR1 (By similarity). Interacts with DDRGK1 and UFL1; the interaction with DDRGK1 is direct (By similarity). Interacts with NCOA1 (By similarity). Interacts with EP300 (By similarity). Part of the ASC-1 complex, that contains TRIP4, ASCC1, ASCC2 and ASCC3 (PubMed:12077347). Interacts with NEK6 (By similarity). Interacts with CSRP1.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207956. 3 interactors.
STRINGi10090.ENSMUSP00000112385.

Structurei

3D structure databases

ProteinModelPortaliQ9QXN3.
SMRiQ9QXN3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni200 – 300Mediates interaction with DDRGK1By similarityAdd BLAST101
Regioni300 – 400Mediates interaction with UFL1By similarityAdd BLAST101

Domaini

The C4-type zinc finger mediates a competitive interaction with UFSP2 and ligand-bound nuclear receptors. It also mediates interaction with the transcriptional coactivators and the basal transcription machinery.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri167 – 219C4-typeAdd BLAST53

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2845. Eukaryota.
ENOG410XTTP. LUCA.
GeneTreeiENSGT00390000005300.
HOGENOMiHOG000006873.
HOVERGENiHBG061618.
InParanoidiQ9QXN3.
OMAiQWVDHTG.
OrthoDBiEOG091G096V.
TreeFamiTF314842.

Family and domain databases

InterProiIPR007374. ASCH_domain.
IPR015947. PUA-like_domain.
IPR009349. Znf_C2HC5.
[Graphical view]
PfamiPF04266. ASCH. 1 hit.
PF06221. zf-C2HC5. 1 hit.
[Graphical view]
SMARTiSM01022. ASCH. 1 hit.
[Graphical view]
SUPFAMiSSF88697. SSF88697. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9QXN3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVAGAAYRE PLVHWCTQQL QKTFALDVSE EIIQYVLSIE NAEEIREYVT
60 70 80 90 100
DLLQGNEGKK GQFIEDLITK WQKNDQEFIS DSFQQCLRKD EILDGQRSVD
110 120 130 140 150
QLKRSRRKGR NKQEVPAFPE PDVAVEVKTP LDLAKAQESN NSVKKKTRFV
160 170 180 190 200
NLYTREGQDK LAVLLPGRHP CDCLGQKHKL INNCLVCGRI VCEQEGSGPC
210 220 230 240 250
LFCGSLVCTN EEQDILQRDS NKSQKLLKKL MSGAETSGKV DVSTKDLLPH
260 270 280 290 300
QESRMKSGLE KAIKHKEKLL EFDRTSIRRT QVIDDESDYF ASDSNQWLSK
310 320 330 340 350
VEREMLQKRE EELRELRHAS RLSKKVTIDF AGRKILEDEN PLAEYHSRLD
360 370 380 390 400
ETIQAIASGT LNQSLVTLDR SCEEPLGVLV NPNMYQASPQ WVDNTGSTPQ
410 420 430 440 450
KKTSLSAGPR LEPSLHQHQL RIQDQEFQEG FDGGWCLSMH QPWASLLVRG
460 470 480 490 500
IKRVEGRSWY TPHRGRLWIA ATGKRPSPQE VSELQATYRL LRGKDVEFPN
510 520 530 540 550
DYPSGCLLGC VDLIDCLSQK QFQEQFPDIS QESDSSFVFI CKNPQEMVVK
560 570 580
FPIKGNPKIW KLDSKIHQGA KKGLMKQNKA V
Length:581
Mass (Da):66,197
Last modified:July 27, 2011 - v2
Checksum:i2CAB3512E3CEDDA7
GO
Isoform 2 (identifier: Q9QXN3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     526-539: FPDISQESDSSFVF → GNWIPRSIKEQRRG
     540-581: Missing.

Note: No experimental confirmation available.
Show »
Length:539
Mass (Da):61,533
Checksum:iFF965B4855F43891
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7A → G in BAC30209 (PubMed:16141072).Curated1
Sequence conflicti388S → P in AAF18440 (PubMed:12077347).Curated1
Sequence conflicti388S → P in AAN23117 (PubMed:12390891).Curated1
Sequence conflicti388S → P in AAH21316 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011109526 – 539FPDIS…SSFVF → GNWIPRSIKEQRRG in isoform 2. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_011110540 – 581Missing in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197574 mRNA. Translation: AAF18440.1.
AF539614 mRNA. Translation: AAN23117.1.
AK039024 mRNA. Translation: BAC30209.1.
AC151906 Genomic DNA. No translation available.
BC021316 mRNA. Translation: AAH21316.1.
CCDSiCCDS23298.1. [Q9QXN3-1]
CCDS52838.1. [Q9QXN3-2]
RefSeqiNP_001164378.1. NM_001170907.1. [Q9QXN3-2]
NP_062771.2. NM_019797.4. [Q9QXN3-1]
XP_006511359.1. XM_006511296.3. [Q9QXN3-1]
XP_006511361.1. XM_006511298.3. [Q9QXN3-1]
XP_006511362.1. XM_006511299.3. [Q9QXN3-1]
XP_017168981.1. XM_017313492.1. [Q9QXN3-1]
XP_017168982.1. XM_017313493.1. [Q9QXN3-2]
UniGeneiMm.208379.

Genome annotation databases

EnsembliENSMUST00000117083; ENSMUSP00000113949; ENSMUSG00000032386. [Q9QXN3-1]
ENSMUST00000119245; ENSMUSP00000112385; ENSMUSG00000032386. [Q9QXN3-1]
ENSMUST00000122410; ENSMUSP00000112866; ENSMUSG00000032386. [Q9QXN3-2]
ENSMUST00000179395; ENSMUSP00000137304; ENSMUSG00000032386. [Q9QXN3-2]
GeneIDi56404.
KEGGimmu:56404.
UCSCiuc009qdz.2. mouse. [Q9QXN3-2]
uc012gvn.1. mouse. [Q9QXN3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF197574 mRNA. Translation: AAF18440.1.
AF539614 mRNA. Translation: AAN23117.1.
AK039024 mRNA. Translation: BAC30209.1.
AC151906 Genomic DNA. No translation available.
BC021316 mRNA. Translation: AAH21316.1.
CCDSiCCDS23298.1. [Q9QXN3-1]
CCDS52838.1. [Q9QXN3-2]
RefSeqiNP_001164378.1. NM_001170907.1. [Q9QXN3-2]
NP_062771.2. NM_019797.4. [Q9QXN3-1]
XP_006511359.1. XM_006511296.3. [Q9QXN3-1]
XP_006511361.1. XM_006511298.3. [Q9QXN3-1]
XP_006511362.1. XM_006511299.3. [Q9QXN3-1]
XP_017168981.1. XM_017313492.1. [Q9QXN3-1]
XP_017168982.1. XM_017313493.1. [Q9QXN3-2]
UniGeneiMm.208379.

3D structure databases

ProteinModelPortaliQ9QXN3.
SMRiQ9QXN3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207956. 3 interactors.
STRINGi10090.ENSMUSP00000112385.

PTM databases

iPTMnetiQ9QXN3.
PhosphoSitePlusiQ9QXN3.

Proteomic databases

PaxDbiQ9QXN3.
PRIDEiQ9QXN3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117083; ENSMUSP00000113949; ENSMUSG00000032386. [Q9QXN3-1]
ENSMUST00000119245; ENSMUSP00000112385; ENSMUSG00000032386. [Q9QXN3-1]
ENSMUST00000122410; ENSMUSP00000112866; ENSMUSG00000032386. [Q9QXN3-2]
ENSMUST00000179395; ENSMUSP00000137304; ENSMUSG00000032386. [Q9QXN3-2]
GeneIDi56404.
KEGGimmu:56404.
UCSCiuc009qdz.2. mouse. [Q9QXN3-2]
uc012gvn.1. mouse. [Q9QXN3-1]

Organism-specific databases

CTDi9325.
MGIiMGI:1928469. Trip4.

Phylogenomic databases

eggNOGiKOG2845. Eukaryota.
ENOG410XTTP. LUCA.
GeneTreeiENSGT00390000005300.
HOGENOMiHOG000006873.
HOVERGENiHBG061618.
InParanoidiQ9QXN3.
OMAiQWVDHTG.
OrthoDBiEOG091G096V.
TreeFamiTF314842.

Miscellaneous databases

ChiTaRSiTrip4. mouse.
PROiQ9QXN3.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032386.
CleanExiMM_TRIP4.
ExpressionAtlasiQ9QXN3. baseline and differential.
GenevisibleiQ9QXN3. MM.

Family and domain databases

InterProiIPR007374. ASCH_domain.
IPR015947. PUA-like_domain.
IPR009349. Znf_C2HC5.
[Graphical view]
PfamiPF04266. ASCH. 1 hit.
PF06221. zf-C2HC5. 1 hit.
[Graphical view]
SMARTiSM01022. ASCH. 1 hit.
[Graphical view]
SUPFAMiSSF88697. SSF88697. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiTRIP4_MOUSE
AccessioniPrimary (citable) accession number: Q9QXN3
Secondary accession number(s): E9QK64, Q8CAD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.